scholarly journals THE GROWTH OF “PISANG RAJA BULU” PLANLETS IN THE LIGHTING OF INCUBATION ROOMS AND GROWTH REGULATOR AGENTS OF BROWNING PREVENTION BY IN VITRO

Agrivet ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 8
Author(s):  
Utami Setyawati ◽  
Ari Wijayani ◽  
Endah Wahyurini
Keyword(s):  
Vitamin C ◽  
Root Length ◽  
Fresh Weight ◽  
Experiment Method ◽  
Two Factors ◽  
Main Plot ◽  
Number Of Leaves ◽  
Plot Design ◽  
Number Of Shoots

The aims of this research were to determine the lighting of incubation rooms and browning prevention agents on the growth of “Pisang Raja Bulu” planlets by in vitro and determine whether there is a best interaction between the incubation rooms lighting and the type of browning prevention agents in the growth of “Pisang Raja Bulu” planlets that plants in vitro. This research was conducted in the Biotechnology laboratory of the Faculty of Agriculture, UPN "Veteran" Yogyakarta on June - August 2018. The research method was a laboratory experiment method compiled with the Split Plot Design of two factors. The first factor as a main plot is the incubation room lighting, which is with light for 90 days, without light for 90 days, without light in the first 45 days, and without light in the last 45 days. The second factor as a sub plot is browning prevention agents, named thidiazuron, activated charcoal, and vitamin C. Each combination of treatments was repeated 3 times. The result showed that there was the best combinations of treatments that is all combination of lighting and vitamin C 0,88 mg / l in terms of browning. The first 45 days lighting treatment gave the best result on the percentage of life, plantlet height, number of shoots, number of leaves, root length, and fresh weight. The treatment of vitamin C 0.88 mg / l gave the best result on the percentage of life, plantlet height, number of shoots, root length, number of roots, fresh weight, and dry weight.Keyword: in vitro, lighting, browning, raja bulu banana.

scholarly journals PERTUMBUHAN DAN DAYA HASIL BAWANG MERAH (Allium ascolanicum L.) DENGAN PEMBERIAN PUPUK KALIUM DAN PUPUK KANDANG AYAM PADA UKURAN BIBIT YANG BERBEDA

2021 ◽  
Vol 12 (1) ◽  
pp. 41
Author(s):  
Armaini Armaini ◽  
Tri Hardianti ◽  
Irfandri Irfandri
Keyword(s):  
Seed Size ◽  
Dry Weight ◽  
Growth And Yield ◽  
Fresh Weight ◽  
Tuber Fresh Weight ◽  
Main Plot ◽  
Number Of Leaves ◽  
The Difference ◽  
The Right ◽  
Plot Design

Shallot productivity can be increased by taking into account the right fertilizer factor and the potential of the seeds used. The study of potassium and manure dosages and seed size were carried out to get the best treatment in increasing growth and yield. The study was conducted in the Experimental Garden of the Faculty of Agriculture, University of Riau, from February to April 2019, using a divided plot design. The main plot is the difference in seed size (> 5.0 - 7.5 and 2.5 - 5.0) g, subplots of doses of manure (0, 15, 30) ton.ha-1 and children of subplots (0 , 200, 400) kg.ha-1. The parameters observed were plant height, number of leaves, age of harvest, number of tubers per clump, diameter of tuber, tuber fresh weight and tuber dry weight, further tests used BNJ level of 5%. The results showed that the highest dry weight of tubers was obtained in the treatment of large seedlings (> 5.0 - 7.5 g) which were given 30 tons. ha-1 of manure and 400 kg.ha-1 of KCl, with an achievement of 4.1 tons.ha-1.

scholarly journals The Effect of Giving Bio Fertilizer and Foliar Fertilizer on the Growth and Yield of Celery (Apium graviolens)

2020 ◽  
Vol 2 (4) ◽  
pp. 482-491
Author(s):  
Wiwik Yunidawati ◽  
Riyanti Riyanti ◽  
Mazlina Mazlina
Keyword(s):  
Plant Height ◽  
Root Length ◽  
Block Design ◽  
Growth And Yield ◽  
Fresh Weight ◽  
Water Parameters ◽  
Foliar Fertilizer ◽  
Randomized Block Design ◽  
Two Factors ◽  
Number Of Leaves

This study aims to determine the effect of bio fertilizers and foliar fertilizers on growth and yield of celery (Apium graviolens). This research was conducted at the Experimental Garden of the Faculty of Agriculture, Amir Hamzah University, Medan, Medan Estate Village, Percut Sei Tuan District with a height of ± 25 meters above sea level, which was conducted from May to July 2020. This study used a factorial Randomized Block Design with two factors studied, namely the first factor was the concentration of bio fertilizer Impro Tanotec (H), which consisted of 4 levels, namely H0 (0 ml/l water), H1 (1,5 ml/l water), H2 (2,5 ml/l water), H3 (3,5 ml/l water), and the second factor is the concentration of foliar fertilizer Growmore (D) which consists of 4 levels, namely D0 (0 gr/lwater), D1 (1 gr/l water), D2 (2 gr/l water), and D3 (3 gr/l water). Parameters observed included plant height (cm), number of leaves, number of stalks, root length (cm), and fresh weight per plant. (gr). The results showed that the concentration of bio fertilizer had a very significant effect on plant height at the age of 20, 40, and 60 days after planting, the number of leaves at the age of 20, 40, and 60 days after planting, the number of stalks at the age of 40 and 60 days after planting, root length, and fresh weight per plant, but did not significantly affect the number of stalks at the age of 20 days after planting. The best bio-fertilizer concentration was obtained in the H3 treatment (3.5 ml/l water). The concentration of foliar fertilizer had a very significant effect on plant height at the age of 20, 40, and 60 days after planting, the number of leaves at the age of 20, 40, and 60 days after planting, the number of stalks at the age of 40 and 60 days after planting, and the fresh weight per plant, had a significant effect on root length, and had no significant effect on the number of stalks at the age of 20 days after planting. The best foliar fertilizer concentration was obtained in D3 treatment (3 gr/l water). The interaction between the concentration of bio fertilizer and foliar fertilizers had a significant effect on the number of leaves at the age of 40 and 60 days after planting, where the best treatment combination was obtained in the H3D3 treatment (bio fertilizer concentration 3.5 ml/l water and foliar fertilizer concentration 3 gr/l water).

scholarly journals The Effects of MYO-inositol and Indole Butyric Acid (IBA) on the Formation of Pineapples Root (Ananas comosus L.) from Sipahutar North Sumatera In Vitro

2018 ◽  
Vol 6 (2) ◽  
pp. 23
Author(s):  
Arisah Hasanah ◽  
Fauziyah Harahap ◽  
Ramlan Silaban
Keyword(s):  
Root Length ◽  
Leaf Length ◽  
Ananas Comosus ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Shoot Height ◽  
Leaf Emergence ◽  
Two Factors ◽  
Number Of Leaves

Background: Rooting of pineapple from Sipahutar in vitro is an alternative to obtain superior seeds in large quantities to meet the needs of farmers and consumers.Objective: The purpose of this study was to investigate the effect of Myo-inositol and IBA on pineapple rooting (Ananas comosus L.) from Sipahutar in vitro.Methods: The research method used a completely randomized factorial design with two factors: Factor I: Myo-inositol consisted of [4] treatment concentrations: M0 = 0 g/l, M0.02 = 0.02 g/l, M0.04 = 0.04 gr/l, M0.06 = 0.06 gr/l. Factor II: IBA growth regulators consisted of [3] treatment concentrations: IB0 = 0 ppm, IB1.5 = 1.5 ppm, IB3 = 3 ppm. The parameters observed were time of root emergence, number of roots, number of leaves, time of leaf emergence, number of tillers, and time of its emergence observed each week, while the height of tillers, shoots, and whole shoots, root length, leaf width and leaf length were observed 12 weeks after planting (WAP).Results: The result of the research showed that root time appeared on [3] WAP. The increasing number of leaves and timing of the puppiesin 1 WAP. Myo-inositol had significant effects on root counts in the 0.06 gr/l and root length on Myo-inositol treatment in the 0.04 g/l. IBA did not have a real effect on the entire observation parameters. The interactions of Myo-inositol and IBA had significant effects on root number, number of leaves, leaf length and tiller height.Conclusions: Myo-inositol and IBA had significant effects on root number, leaf number, leaf length and shoot height of pineapple’s explant from Sipahutar. 

scholarly journals Keragaan tiga jenis planlet anggrek Phalaenopsis asal Protocorm yang diinduksi Ethyl Methyl Sulfonate (EMS) secara in vitro

Kultivasi ◽  
2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Romiyadi Romiyadi ◽  
Ai Komariah ◽  
Suseno Amien
Keyword(s):  
Root Length ◽  
Variable Number ◽  
Leaf Length ◽  
Ethyl Methyl ◽  
Randomized Design ◽  
Methyl Sulfonate ◽  
Two Factors ◽  
Number Of Leaves ◽  
Ethyl Methyl Sulfonate

Abstract. This research was conducted to determine the effect of concentration of Ethyl Methyl Sulfonate (EMS) to performance of three types of Phalaenopsis Planlets and to know the optimum concentration of EMS on any type of Phalaenopsis orchids. The experiment was conduc-ted at Tissue Culture Laboratory of the Faculty of Agriculture, Winaya Mukti University, Sumedang. It used a completely randomized design (CRD) with factorial pattern, that consisted of two factors and replicated twice.The first factor was the type of Phalaenopsis that resulted form hybridization which consisted of v1 (Phalaenopsis 717 X Phalae-nopsis Fire Bird), v2 (Phalaenopsis Tianong Rose X Sibling), and v3 (Phalaenopsis Luchia Pink X Phalae-nopsis Chain Xen Mammon). The second factor was the concentration of EMS that consisted of e0 (0% EMS/control), e1 (0,05% EMS), e2 (0,10% EMS), e3 (0,15% EMS), e4 (0,20% EMS), and e5 (0,25% EMS). Explant protocorm of three types of Phalae-nopsis soaked in a solution of EMS by each treat-ment for 3 hours, and cultured on MS medium Basal Modified Multiplication Shoot for 10 weeks. The experimental results showed that there are interaction between the three types of Phalae-nopsis result of a cross with a concentration of EMS to variable number of roots. Orchids P. 717 X P. Fire Bird had higher  number of leaves, number of roots, leaf leang, and root length than the other. The result showed that there were interaction between three species of Phalaenopsis orchid from the crossing with EMS concentration on root variables. Orchid P. 717 X P. Fire Bird has the number of leaves, the number of roots, the number of shoots, leaf length and root length better than other types. EMS concentrations independently at all levels of treatment can not increase the number of leaves, the number of roots, fresh weight of planlet, leaf length, and root length.Keywords: Phalaenopsis, the concentration of EMS, in vitro cultureSari. Penelitian ini mempelajari dan mengetahui pengaruh konsentrasi Ethyl Methyl Sulfonate (EMS) terhadap keragaan planlet tiga jenis anggrek Phalaenopsis asal protocorm dan mencari konsentrasi optimum EMS untuk setiap jenis anggrek Phalae-nopsis secara in vitro.Percobaan dilaksanakan di Laboratorium Kultur Jaringan Fakultas Pertanian Universitas Winaya Mukti Sumedang. Eksperimen menggunakan Rancangan Acak Lengkap (RAL) Pola Faktorial, yang terdiri atas dua faktor dan diulang sebanyak dua kali.Faktor pertama adalah jenis anggrek Phalaenopsis hasil persilangan yang terdiri atas tiga jenis, yaitu v1 (Phalaenopsis 717 X Phalaenopsis Fire Bird), v2 (Phalaenopsis Tianong Rose X Sibling), dan v3 (Phalaenopsis Luchia Pink X Phalaenopsis Chain Xen Mammon). Faktor kedua adalah EMS yang terdiri atas enam taraf perlakuan, yaitu e0 (0% EMS/kontrol), e1 (0,05% EMS), e2 (0,10% EMS), e3 (0,15% EMS), e4 (0,20% EMS), dan e5 (0,25% EMS). Eksplan berupa protocorm dari tiga jenis anggrek Phalaenopsis hasil persilangan yang direndam dalam larutan EMS berdasarkan masing-masing perlakuan selama 3 jam, dan dikulturkan pada media MS Modified Multiplication Shoot Basal selama 10 minggu.Hasil penelitian menunjukkan terjadi interaksi antara tiga jenis anggrek Phalaenopsis hasil persilangan dengan konsentrasi EMS terhadap variabel jumlah akar.Anggrek P. 717 X P.Fire Bird memiliki jumlah daun, jumlah akar, jumlah tunas, panjang daun dan panjang akar yang lebih baik dibandingkan jenis lainnya. Konsentrasi EMS secara mandiri pada semua taraf perlakuan tidak dapat meningkatkan jumlah daun, jumlah akar, bobot segar planlet, panjang daun, dan panjang akar.Kata Kunci: Phalaenopsis, Konsentrasi EMS, dan Budidaya In Vitro

scholarly journals THE EFFECT OF GIVING GIBBERELLIN ON THE GROWTH AND YIELD OF CHERRY TOMATOES (Lycopersicon esculentum var. cerasiforme) IN VARIOUS OF PLANTING MEDIA WITH SUBSTRATE HYDROPONIC SYSTEMS

Agrivet ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 25
Author(s):  
Mochammad Febrianto ◽  
Supono Budi Sutoto ◽  
Suwardi Suwardi
Keyword(s):  
Plant Growth ◽  
Vitamin C ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Root Length ◽  
Growth And Yield ◽  
Observation Data ◽  
Fresh Weight ◽  
Cherry Tomatoes ◽  
Number Of Shoots

The efforts to increase cherry tomatoes production can be done by increasing the forming of ovaries. This effort can be helped by utilizing Zat Pengatur Tumbuh (ZPT) or Plant Growth Regulators. The Gibberellin is a growth regulator which has the function of flowering initiation and preventing the flowers from falling off. In addition to PGR, planting media is important for the growth of each plant. The planting media also has function as the root container, and the media which can absorb nutrient solution when it is doused or dripped. This research was conducted at The Greenhouse, Sukun Street, Karangbendo, Jaranan, Banguntapan, Bantul, Daerah Istimewa Yogyakarta. The study was conducted in May to August 2018. The experiment method uses polybag with Split Plot Design. In this experiment, there are two factors, and it repeated three times. The first factor as the main plot is the Plant Growth Regulators concentration of Gibberellin GA3 with the level (G0 : 0 ppm, G1 : 50 ppm, G2 : 75 ppm, G3 100 ppm). The second factor as the sub-plot is the type of planting media (M1: Husk Charcoal, M2: Malang Sand, M3: Husk Charcoal + Cocopeat (1:1)). The observation data is analyzed for its diversity at level of 5%. To find out the differences between the treatments, the method which is used is Duncan Multiple Range Test (DMRT) at level of 5%. The result shows that there is a significant effect of Gibberellin Plant Growth Regulators utilizing and types of planting media to the growth and yield of cherry tomatoes. The best Gibberellin concentration is 100 ppm, and the best media is the Husk Charcoal + Cocopeat (1:1).in the last 45 days. The second factor as a sub plot is browning prevention agents, named thidiazuron, activated charcoal, and vitamin C. Each combination of treatments was repeated 3 times. The result showed that there was the best combinations of treatments that is all combination of lighting and vitamin C 0,88 mg / l in terms of browning. The first 45 days lighting treatment gave the best result on the percentage of life, plantlet height, number of shoots, number of leaves, root length, and fresh weight. The treatment of vitamin C 0.88 mg / l gave the best result on the percentage of life, plantlet height, number of shoots, root length, number of roots, fresh weight, and dry weight.Keyword: Gherry tomatoes, substrate hydroponic, gibberellin, planting media.

scholarly journals Light in intermediate acclimatization of in vitro germinated seedlings of Dendrobium phalaenopsis Deang Suree

2015 ◽  
Vol 45 (2) ◽  
pp. 231-237 ◽  
Author(s):  
José Carlos Sorgato ◽  
Yara Brito Chaim Jardim Rosa ◽  
Jackeline Schultz Soares ◽  
Camila Soares Rosa Lemes ◽  
Gisele Garcia de Sousa
Keyword(s):  
White Light ◽  
Root Length ◽  
Red Light ◽  
Fluorescent Light ◽  
Fresh Weight ◽  
Survival Percentage ◽  
Number Of Leaves ◽  
Weight Number ◽  
Better Than

The success in micropropagation of Dendrobium phalaenopsis Deang Suree is high, but when transplanted into the greenhouse, their survival is minimal. To increase survival in production in the present study it was evaluated the effect of intermediate acclimatization for 30 days in a grow room utilizing the following luminosity conditions: 1- white fluorescent light (B) (18.9µmol m-2 s-1); 2- white fluorescent light + red fluorescent light (GRO-LUX(r)) (BV) (14.85µmol m-2 s-1); 3- red fluorescent light (GRO-LUX(r)) (V) (9.45µmol m-2 s-1) and the control plants were accommodated directly in a greenhouse (162.0µmol m-2 s-1). After this the leaves were characterized anatomically and the plants transferred to the control greenhouse. It was evaluated survival percentage and final number of roots, and calculated the relations between the final and initial values of fresh weight, number of leaves, length and diameter of the largest pseudo bulb, number of pseudo bulbs and longest root length. Only plants submitted to red light, were statistically better than the control in relation to the survival percentage and in relation to fresh weight, while the control showed a higher number of roots that plants acclimatized in this luminosity conditions. Intermediate acclimatization, using red light or red + white light, is recommended for D. phalaenopsis Deang Suree.

scholarly journals The shoot explant types effect of eleven stevia (Stevia rebaudiana Bertoni)

Jurnal Agro ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 142-152
Author(s):  
Suseno Amien ◽  
Arini Zahra Azhari ◽  
Citra Bakti ◽  
Haris Maulana
Keyword(s):  
Stevia Rebaudiana ◽  
Shoot Tip ◽  
Shoot Height ◽  
Stevia Rebaudiana Bertoni ◽  
Randomized Design ◽  
Two Factors ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Number Of Shoots

Explants play an important role in thepropagation system of stevia (Stevia rebaudiana Bertoni). A Completely Randomized Design (CRD) was used in this experiment with factorial pattern consisting of two factors, namely three types of explants (shoot tip, first node, and second node) and eleven accessions of stevia namely a1 (Bogor), a2 (Garut), a3 (Canada), a4 (Tawangmangu), a5 (STG1), a6 (SBG 4), a7 (SBG 10), a8 (SGB 2), a9 (BR5), a10 (SGR 7.5), a11 (TR 3.5). The results showed that shoot tip explant was the best explant than first node and second node for the number of shoots (18.11 shoots), number of leaves (93.49 leaves) and wet weight (3.56 grams). The best accession of shoot height was a10(SGR 7.5) (19.95 cm), the highest number of shoots wasa7(SBG 10) (21.87 shoots), the highest number of leaves wasa7(SBG 10) (138.00 leaves), the heaviest wet weight wasa7(SBG 10) (3.56 grams), the highest leaf chlorophyll content was a10 (SGR 7.5) (0.63 µg mL-1). Accessions with the fastest root initiation time at the rooting stage was a11 (TR 3.5) (4.00 DAC), the highest number of roots wasa10 (TR 3.5) (27.11 roots), the best root length wasa2(Garut) (4.51 cm). Information on the best explant types and stevia accessions in the in-vitro multiplication stage can be used as the basis for stevia breeding programs in Indonesia.AbstractEksplan berperan penting dalam sistem perbanyakan Stevia (Stevia rebaudiana Bertoni). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan pola faktorial yang terdiri dari dua faktor yaitu tiga jenis eksplan (ujung pucuk, ruas pertama, dan ruas kedua) dan sebelas aksesi stevia yaitu a1 (Bogor), a2 (Garut), a3 (Canada), a4 (Tawangmangu), a5 (STG1), a6 (SBG 4), a7 (SBG 10), a8 (SGB 2), a9 (BR5), a10 (SGR 7.5), a11 (TR 3.5). Hasil penelitian menunjukkan bahwa eksplan ujung pucuk merupakan eksplan terbaik dibandingkan buku pertama dan kedua untuk jumlah tunas (18,11 tunas), jumlah daun (93,49 daun) dan berat basah (3,56 gram). Aksesi terbaik pada tinggi pucuk adalah a10(SGR 7.5) (19,95 cm), jumlah pucuk tertinggi a7 (SBG 10) (21,87 pucuk), jumlah daun terbanyak a7 (SBG 10) (138.00 helai daun), bobot basah terberat a7 (SBG 10) (3,56 gram), kandungan klorofil daun tertinggi adalah GR 7,5 (0,63 µg mL-1). Aksesi dengan waktu inisiasi akar tercepat pada tahap perakaran adalah TR 3,5 (4,00 HST), jumlah akar terbanyak adalah TR 3,5 (27,11 akar), panjang akar terbaik adalah a2(Garut) (4,51 cm). Informasi jenis eksplan dan aksesi stevia terbaik pada tahap multiplikasi in-vitro dapat dijadikan dasar program pemuliaan stevia di Indonesia.

scholarly journals Coverage effect on hardening of arrow cane (Gynerium sagitatum Aubl.) micropropagated plants

2021 ◽  
Vol 74 (2) ◽  
Author(s):  
Isidro Elias Suarez Padron ◽  
Diego Pico Vellojin ◽  
Claudia Marcela Lopez Diaz
Keyword(s):  
The Other ◽  
Plastic Film ◽  
Ex Vitro ◽  
Tukey Test ◽  
Split Plot ◽  
Micropropagated Plants ◽  
Main Plot ◽  
Plot Design ◽  
Number Of Shoots

To evaluate the effect of plastic film coverage on ex vitro acclimatization of arrow cane (Gynerium sagitatum Aubl.), shoots of “Criolla”,  Martinera” and “Costera” cultivars were in vitro micropropagated in a medium supplied with 6-Benzylaminopurine and half of them were rooted in a medium with 1-Naphtalene acetic acid. Rooted and unrooted shoots were transplanted in a shade house with fog irrigation, into plastic trays (72 clusters per tray), using peat as substrate and half of them was covered with translucent plastic film during 5 days after transplant while the other half was maintained uncovered. The experiment consisted of a three-way factorial arrangement with 12 treatments distributed with a split-plot design where tray coverage was the main plot, cultivars were the split, and rooting condition was the split-plot. Each treatment (36 clusters) was repeated three times for a total of 1296 experimental units. After 40 days in the shade house, the survival rate was calculated, and plant heigth, number of shoots and number of roots data were analyzed by ANOVA (P<0.05) and means were separated by Tukey test (P<0.05). Plant survival was complete (100%) regardless of genotype, rooting, or coverage condition. Transferring plant into uncovered trays statistically resulted in higher levels for plant height, number of shoots, and number of roots. Ex vitro adaptation of micropropagated arrow cane plants without plastic film covers increased plant growth and reduced labor.

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

scholarly journals PERTUMBUHAN BIBIT JERUK ASAL KUOK HASIL OKULASI PADA BERBAGAI TINGKAT NAUNGAN DAN UMUR BATANG BAWAH

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Gusriani Gusriani ◽  
Tiara Septirosya ◽  
Ahmad Darmawi
Keyword(s):  
Bud Burst ◽  
State University ◽  
Time Length ◽  
Experimental Field ◽  
Split Plot ◽  
Two Factors ◽  
Number Of Leaves ◽  
Burst Time ◽  
Plot Design

Citrus that comes from Kuok is one of famous local comodity in Riau Province with limited production. To improve the production of citrus it can be done by serving the superior seed throught budding. Rootstock aged and shading levels give effect to the success of budding process. The aims of the study were to obtain the best shade level, suitable rootstock aged and the interaction of rootstock aged with shade level on budding success. This study was conducted on December 2018 to March 2019 at the Experimental Field, Faculty of Agriculture and Animals Science, Islam State University of Sultan Syarif Kasim Riau. This study used split plot design with two factors, shading levels (0%, 50%, 70%) and rootstock aged (4, 8, 12 month) with three replications. The parameters this measured in this study were growth percentage of buds, percentage of sprout, percentage of dormancy, bud burst time, length of buds and number of leaves. The results showed that shade level of 0% and the age of rootstock 4 months produced the best growth of citrus seedlings. There is interaction between the shade level 0% and rootstock aged 12 month to the growth of the budding citrus seedlings. 

Sign in / Sign up

Export Citation Format

Share Document