scholarly journals Effect of Cow Urine and IBA on the Growth of Sweet Leaf (Sauropus androgynus (L.) Merr.) Cuttings

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Fawziyah Nurshabrina ◽  
Arifah Rahayu ◽  
Oktavianus Lt

Sweet leaf (Sauropus androgynus (L.) Merr.), a leaf vegetable that is generally propagated vegetatively with cuttings. This study aims to determine the effect of accession and administration of natural and synthetic auxin growth regulators on the growth of sweet leaf cuttings. This study uses factorial Randomized Complete Design consisting of two factors, namely accession (Banten from Pandeglang, Bogor from Tajur, and Cianjur from Cugenang) and auksin combination (IBA 100%R, urine 25%R + IBA 75%R, urine 50%R + IBA 50%R, urine 75%R + IBA 25%R, and urine 100%R). The results showed that the growth of Cianjur accession had the best results on the percentage of growth cuttings, the percentage of shoot buds, shoot height, number of shoots, number of leaves, percentage of rooted cuttings, and number of roots compared to Banten and Bogor accessions. Giving urine 100%R significantly increases plant height, shoot height, number of shoots, and number of sweet leaf plant roots compared with other auxin combinations. The combination treatment of auxin and accession showed results that were not significantly different from the variable root length and weight of the harvest. In sweet leaf plants, cow urine can replace the use of IBA (Indole Butyric Acid). Keywords: Sauropus androgynus, vegetative, urine, IBA

2021 ◽  
Vol 905 (1) ◽  
pp. 012036
Author(s):  
Nandariyah ◽  
L S Mahmudah ◽  
R B Arniputri ◽  
A T Sakya

Abstract Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.


2018 ◽  
Vol 6 (2) ◽  
pp. 23
Author(s):  
Arisah Hasanah ◽  
Fauziyah Harahap ◽  
Ramlan Silaban

Background: Rooting of pineapple from Sipahutar in vitro is an alternative to obtain superior seeds in large quantities to meet the needs of farmers and consumers.Objective: The purpose of this study was to investigate the effect of Myo-inositol and IBA on pineapple rooting (Ananas comosus L.) from Sipahutar in vitro.Methods: The research method used a completely randomized factorial design with two factors: Factor I: Myo-inositol consisted of [4] treatment concentrations: M0 = 0 g/l, M0.02 = 0.02 g/l, M0.04 = 0.04 gr/l, M0.06 = 0.06 gr/l. Factor II: IBA growth regulators consisted of [3] treatment concentrations: IB0 = 0 ppm, IB1.5 = 1.5 ppm, IB3 = 3 ppm. The parameters observed were time of root emergence, number of roots, number of leaves, time of leaf emergence, number of tillers, and time of its emergence observed each week, while the height of tillers, shoots, and whole shoots, root length, leaf width and leaf length were observed 12 weeks after planting (WAP).Results: The result of the research showed that root time appeared on [3] WAP. The increasing number of leaves and timing of the puppiesin 1 WAP. Myo-inositol had significant effects on root counts in the 0.06 gr/l and root length on Myo-inositol treatment in the 0.04 g/l. IBA did not have a real effect on the entire observation parameters. The interactions of Myo-inositol and IBA had significant effects on root number, number of leaves, leaf length and tiller height.Conclusions: Myo-inositol and IBA had significant effects on root number, leaf number, leaf length and shoot height of pineapple’s explant from Sipahutar. 


Jurnal Agro ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 142-152
Author(s):  
Suseno Amien ◽  
Arini Zahra Azhari ◽  
Citra Bakti ◽  
Haris Maulana

Explants play an important role in thepropagation system of stevia (Stevia rebaudiana Bertoni). A Completely Randomized Design (CRD) was used in this experiment with factorial pattern consisting of two factors, namely three types of explants (shoot tip, first node, and second node) and eleven accessions of stevia namely a1 (Bogor), a2 (Garut), a3 (Canada), a4 (Tawangmangu), a5 (STG1), a6 (SBG 4), a7 (SBG 10), a8 (SGB 2), a9 (BR5), a10 (SGR 7.5), a11 (TR 3.5). The results showed that shoot tip explant was the best explant than first node and second node for the number of shoots (18.11 shoots), number of leaves (93.49 leaves) and wet weight (3.56 grams). The best accession of shoot height was a10(SGR 7.5) (19.95 cm), the highest number of shoots wasa7(SBG 10) (21.87 shoots), the highest number of leaves wasa7(SBG 10) (138.00 leaves), the heaviest wet weight wasa7(SBG 10) (3.56 grams), the highest leaf chlorophyll content was a10 (SGR 7.5) (0.63 µg mL-1). Accessions with the fastest root initiation time at the rooting stage was a11 (TR 3.5) (4.00 DAC), the highest number of roots wasa10 (TR 3.5) (27.11 roots), the best root length wasa2(Garut) (4.51 cm). Information on the best explant types and stevia accessions in the in-vitro multiplication stage can be used as the basis for stevia breeding programs in Indonesia.AbstractEksplan berperan penting dalam sistem perbanyakan Stevia (Stevia rebaudiana Bertoni). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan pola faktorial yang terdiri dari dua faktor yaitu tiga jenis eksplan (ujung pucuk, ruas pertama, dan ruas kedua) dan sebelas aksesi stevia yaitu a1 (Bogor), a2 (Garut), a3 (Canada), a4 (Tawangmangu), a5 (STG1), a6 (SBG 4), a7 (SBG 10), a8 (SGB 2), a9 (BR5), a10 (SGR 7.5), a11 (TR 3.5). Hasil penelitian menunjukkan bahwa eksplan ujung pucuk merupakan eksplan terbaik dibandingkan buku pertama dan kedua untuk jumlah tunas (18,11 tunas), jumlah daun (93,49 daun) dan berat basah (3,56 gram). Aksesi terbaik pada tinggi pucuk adalah a10(SGR 7.5) (19,95 cm), jumlah pucuk tertinggi a7 (SBG 10) (21,87 pucuk), jumlah daun terbanyak a7 (SBG 10) (138.00 helai daun), bobot basah terberat a7 (SBG 10) (3,56 gram), kandungan klorofil daun tertinggi adalah GR 7,5 (0,63 µg mL-1). Aksesi dengan waktu inisiasi akar tercepat pada tahap perakaran adalah TR 3,5 (4,00 HST), jumlah akar terbanyak adalah TR 3,5 (27,11 akar), panjang akar terbaik adalah a2(Garut) (4,51 cm). Informasi jenis eksplan dan aksesi stevia terbaik pada tahap multiplikasi in-vitro dapat dijadikan dasar program pemuliaan stevia di Indonesia.


1970 ◽  
Vol 35 (3) ◽  
pp. 507-514 ◽  
Author(s):  
H Khatun ◽  
MM Khatun ◽  
MS Biswas ◽  
MR Kabir ◽  
M Al-Amin

The experiment was conducted to investigate the combined effect of different plant growth regulators and charcoal supplementation in MS medium on growth and development of plantlets regenerated from protocorm like bodies (PLBs) of hybrid orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations with charcoal supplementation was studied. The result revealed that the use of different growth regulators had significant effect on different parameters studied. The maximum weight of PLBs (5.123 g) was obtained from the combination of BAP + IBA at 1.0 mg/l each. The highest shoot height (3.239 cm) and maximum number of rooted plantlets (4.473) was obtained from 1.0 mg/l each of BAP + NAA combination. The maximum number of leaves (3.490) and the maximum length of leaves (1.946 cm) were obtained from 1.0 mg/l each of BAP + IBA and the highest leaf width (1.166 cm) was obtained from 0.5 mg/l BAP +1.0 mg/l IBA combination. The highest root length was obtained from 0.5 mg/l each of BAP + IAA and the maximum number of regenerated plantlets (20) was obtained from 0.5 mg/l IAA + 1.0 mg/l IBA combination. However, the maximum fresh weight of single shoot (0.219 g) and the maximum number of roots per plantlet (6.300) was obtained from 1.0 mg/I each of IAA + IBA combination. Keywords: Dedrobium; orchid; hybrid; In vitro growth. DOI: 10.3329/bjar.v35i3.6457Bangladesh J. Agril. Res. 35(3) : 507-514


Author(s):  
Enik Akhiriana ◽  
Samanhudi ◽  
Ahmad Yunus

Tribulus (Tribulus terrestrisL.) is a medicinal plant with considerable implementation such as aphrodisiac and anti‑inflammation drugs. This research was conducted to study the effectivity of coconut water and IAA (Indole‑3‑acetic acid) application on various concentration as the growth regulator for tribulus growth on thein vitroand its effect toward observation variables. The material used were cotyledon from tribulus embryo and MS (Murashige and Skoog) medium. The research method used a Completely Randomized Design with first factor of coconut water concentration of 0 mL L-1, 50 mL L-1, 100 mL L-1, and 150 mL L-1and IAA concentration of 0 ppm, 0.15 ppm, 0.20 ppm, and 0.25 ppm as the second factor. Observed variables were shoots emergence time, number of shoots, shoot height, number of leaves, roots emergence time, number of roots and regression between observation variables. The result showed that the combination of 150 mL L-1coconut water and 0.25 ppm IAA gave the highest shoot height, roots emergence time, and number of roots. Coconut water treatment on 150 mL L-1concentration alone gave the best result on shoots emergence time, number of shoots, and number of leaves, while IAA concentration of 0.25 ppm independently gave the highest number of leaves. Regression analysis result indicate that the number of roots has a positive correlation with shoots height, number of shoots and number of leaves.


2016 ◽  
Vol 22 (2) ◽  
pp. 202
Author(s):  
Roseane Rodrigues De Souza ◽  
Ângela Maria Pereira Nascimento ◽  
Patrícia Duarte de Oliveira Paiva ◽  
Elka Fabiana Aparecida Almeida ◽  
Paulo Roberto Correa Landgraft

Intensity and spectral quality of the radiation can be manipulated with the use of neutral or color shading screens in growing plants, and it can promote physiological and morphological changes. Therefore, the purpose was evaluating the effects of different shading levels, screens spectral quality, and spacing in the development of cv. Jungle King and cv. Jungle Queen gingers. Rhizomes were planted in 5 cultivation environments (full sun, black screen 50%, black screen 30%, blue screen 50% and red screen 50% shading) and 2 spacing (0.8 x 1.0 m and 0.8 x 1.5 m). The experimental design was completely randomized in a factorial 5x2x2x7, with 5 environments of cultivation, 2 cultivars, 2 spacing between plants and 7 times of evaluation (55, 120, 200, 255, 285, 335 e 370 days after planting (DAP)). The evaluations were shooting index, number of shoots per plant, shoot height and number of leaves per shoot. Shooting of cv. Jungle Queen was earlier compared to cv. Jungle King. Spacing did not influence in the initial development of plants. Screens with 50% shading, in blue or red colors, enhanced alpinia development.


2020 ◽  
Vol 12 (2) ◽  
pp. 67
Author(s):  
ENDJO DJAUHARIYA ◽  
MONO RAHARDJO ◽  
AGUS SUDIMAN ◽  
SUKARMAN SUKARMAN

ABSTRAK<br />Tanaman kemukus (Piper cubeba LINN.) sudah dikenal sejak<br />jaman dahulu sebagai tanaman obat, rempah, pengharum dan penyedap<br />masakan. Di Jawa Tengah perbanyakan tanaman kemukus pada umumnya<br />dilakukan melalui setek panjang yang terdiri dari 8 - 14 ruas. Perbanyakan<br />dengan cara demikian dianggap tidak ekonomis, oleh karena itu perlu<br />dicari cara perbanyakan yang efisien dan efektif. Percobaan pengaruh<br />macam setek dan komposisi media tumbuh terhadap daya tumbuh dan<br />vigor bibit dilakukan dengan tujuan untuk mendapatkan teknologi<br />perbanyakan kemukus. Percobaan dilakukan di Kebun Percobaan<br />Cimanggu, Balai Penelitian Tanaman Rempah dan Obat Bogor dari bulan<br />September sampai dengan Desember 2003. Percobaan menggunakan<br />rancangan acak kelompok yang disusun secara faktorial dengan 2 faktor<br />dan 3 ulangan. Sebagai faktor pertama adalah 3 macam setek pendek 3<br />ruas yaitu : (1) setek bertapak, (2) setek sulur panjat dan (3) setek cabang<br />buah. Faktor kedua adalah tiga perlakuan komposisi media tumbuh terdiri<br />dari (tanah + pupuk kandang + pasir) dengan perbandingan: (a) 1:1:1, (b)<br />2:1:1, dan (c) 3:1:1. Media dimasukkan ke dalam polibag ukuran 10 x 12<br />cm. Variabel yang diamati meliputi persentase daya tumbuh, panjang<br />tunas, jumlah daun, bobot kering tunas, jumlah akar, panjang akar dan<br />bobot kering akar. Hasil percobaan menunjukkan bahwa vigor bibit yang<br />diekspresikan oleh persentase daya tumbuh, pertumbuhan tunas dan akar<br />tidak nyata dipengaruhi oleh interaksi jenis setek dan komposisi media<br />tumbuh. Jenis setek berpangaruh nyata terhadap semua variabel yang<br />diamati, kecuali terhadap jumlah daun. Jenis setek yang berasal dari setek<br />bertapak dan sulur panjat manghasilkan persentase daya tumbuh 68,40%<br />dan 62,00%, panjang tunas 2,87 cm dan 4,70 cm, bobot kering tunas 0,13<br />g dan 0,14 g, jumlah akar 5,95 dan 5,76 dan bobot kering akar 0,05 g dan<br />0.05 g, lebih baik dibandingkan setek cabang buah. Jenis media tumbuh<br />hanya berpengaruh nyata terhadap bobot kering tunas tapi tidak<br />berpengruh nyata terhadap variabel lainnya. Bobot kering tunas yang<br />terbaik didapat pada komposisi media tumbuh tanah + pupuk kandang +<br />pasir (1 : 1 : 1) (0,14 g) dan terendah pada komposisi media tumbuh tanah<br />+ pupuk kandang + pasir (3 : 1 : 1) (0.11 g).<br />Kata kunci : Kemukus, Piper cubeba LINN, bahan tanaman, macam<br />setek, media tumbuh, daya tumbuh, Jawa Barat<br />ABSTRACT<br />Effect of cutting materials and growth media on the<br />growth of cubeba cuttings<br />In Indonesia, cubeba pepper plant (Piper cubeba LINN) has been<br />known for years as a traditional medicine, spice, fragrant, and seasonings.<br />In Central of Java, it is usually propagated by using eight or fourteen node<br />cuttings which is not an economical practice. The research on cutting<br />materials and growth media was conducted in Cimanggu Experimental<br />Garden of the Indonesian Spice and Medicinal Crops Research Institute<br />from September to December 2003. The objective of the research was to<br />find out an appropriate propagation technology of cubeba. The research<br />used two factors and three replications which was arranged in a<br />randomized completely block design. The first factor was three kinds of<br />cutting nodes, i.e. (1) attached-rooted cuttings (2) vegetative branch and<br />(3) generative branch. The second factor was three kinds of media<br />compositions of soil, dung manure and sand (1) 1:1:1, (2) 2:1:1 and (3)<br />3:1:1. Observations were conducted on the percentage of budding, length<br />of bud, number of leaves, number of roots, length of root, dry weight of<br />the roots, and the shoot. The results of the research indicated that the vigor<br />of seedlings which was expressed by germination percentage, growth of<br />seedlings, and growth of root, did not significantly affected by the<br />interaction between kinds of cuttings and media composition. However,<br />the kinds of cuttings significantly affected all variables, except the number<br />of leaves. Cubeba seedlings originated from attached-rooted cuttings and<br />vegetative branch had higher germination percentage i.e. 68.40% and<br />62.00%, length of shoot 2.87 cm and 4.70 cm, dry weight of shoot 0.13 g<br />and 0.14 g, number of roots 5.95 and 5.76, length of root 7.32 cm and 7.27<br />cm, and dry weight of root 0.05 g and 0.05 g, compared to the cubeba<br />seedlings originated from generative branch. Media composition was<br />significantly effected only on dry weight of shoots. The highest dry weight<br />of shoot was resulted from composition of soil, dung manure and sand<br />1:1;1 (0.14 g), while the lowest was found on ratio media composition of<br />soil, dung manure and sand 3:1:1 (0.11g).<br />Key words : Cubeba, Piper cubeba LINN, plant material, cutting<br />materials, growth media, growth, West Java


2020 ◽  
Vol 12 (4) ◽  
pp. 135
Author(s):  
MELATI MELATI ◽  
DEVI RUSMIN ◽  
SUKARMAN SUKARMAN

ABSTRAK<br />Dalam upaya pengembangan nilam (Pogostemon cablin) di daerah<br />yang jaraknya jauh dari kebun induk, pengadaan benih nilam yang berkua-<br />litas menjadi masalah yang serius, karena bibit akan cepat mengalami<br />penurunan kualitas selama transportasi. Untuk itu dilaksanakan penelitian<br />yang bertujuan untuk mengetahui pengaruh lama simpan setek berakar<br />nilam terhadap pertumbuhan. Percobaan dilaksanakan di rumah kaca Balai<br />Penelitian Tanaman Rempah dan Obat (Balittro) dari bulan April –<br />Agustus 2004. Percobaan disusun dalam rancangan petak terbagi (RPT)<br />dengan 3 ulangan. Petak utama (main plot) adalah 2 jenis setek nilam<br />yaitu: (1) setek berdaun dan (2) setek tidak berdaun. Anak petak (sub plot)<br />adalah lama penyimpanan setek yaitu: (1) setek langsung ditanam<br />(kontrol), (2) setek disimpan 1 hari, (3) setek disimpan 3 hari, (4)setek<br />disimpan 5 hari dan, (5) setek disimpan 7 hari. Pengamatan dilakukan<br />sejak tanaman berumur 2 minggu sampai tanaman berumur 8 minggu.<br />Parameter yang diamati meliputi pertumbuhan tanaman (tinggi, jumlah<br />daun dan jumlah tunas), bobot kering (batang, daun, akar). Hasil perco-<br />baan menunjukkan bahwa persentase hidup setek nilam berakar (setek<br />berdaun dan setek tidak berdaun) masih 100% setelah disimpan selama 7<br />hari. Hampir dari seluruh parameter pertumbuhan (tinggi tanaman, jumlah<br />cabang, jumlah daun) yang diamati menunjukkan bahwa pertumbuhan<br />bibit setek berdaun lebih baik dibandingkan dengan setek yang tidak<br />berdaun.<br />Kata kunci : Nilam, Pogostemon cablin Benth, bibit, setek berakar,<br />penyimpanan, pertumbuhan, Jawa Barat<br />ABSTRACT<br />Effect of storage periods of rooted cutting on the growth<br />of patchouli (Pogostemon cablin Benth)<br />Providing high quality of patchouli (Pogostemon cablin Benth)<br />seedlings is necessary to support the development of patchouli plants. In<br />the new developing area transportation become serious problems (high<br />cost transportation), therefore some alternative solution is reducing the<br />transportation cost without reducing the quality of the seedlings. Base on<br />the problems, this experiment was conducted. The objective of this<br />experiment was to study the effect of storage periods of rooted cuttings on<br />the growth of patchouli plant. The experiment was conducted in the green<br />house of Indonesian Spice and Medicinal Crops Research Institute<br />(ISMECRI), from April to August 2004. The experiment was arranged in<br />a split-plot design with 3 replications. The main plot was 2 kinds of cutting<br />there were: (1) leaf cutting and (2) non leaf cutting. The sub plot was 5<br />different storage periods, there were: (1) control (no storage), (2)1 day<br />storage, (3) 3 day storage, (4) 5 day storage, (5) 7 day storage. The<br />variables observed were plant growth (plant height, number of leaves and<br />number of shoots), dry weight of stems, leaves and roots. The results of<br />experiment indicated that after 7 day storage, rooted cuttings of patchouli<br />were still 100% viable. The growth of patchouli from leaf cutting was<br />significantly different from non leaf cutting. Patchouli plant from leaf<br />cutting produced higher plant height, number of leaves, number of shoots<br />and dry weight of plants compared to those of patchouli plants from non<br />leaf cuttings. Storage period significantly affected the height of plants<br />however it did not significantly affected the number of leaves, number of<br />shoots and dry weight of plants.<br />Key words: Pacthouli, Pogostemon cablin, seedlings, rooted cutting,<br />storage, growth, West JavE


Author(s):  
Yuliatul Muslimah ◽  
Sumeinika Fitria Lizmah ◽  
Nur Fayanti

Propagation of jasmine plants by cuttings often experience obstacles, especially to grow roots. Proper planting media and application of growth regulators (PGR) have an important role in the growth and development of plant cuttings roots. The purpose of this study was to determine the effect of planting media types and PGR on the growth of jasmine cuttings. This research was conducted in the experimental garden of the Faculty of Agriculture, Teuku Umar University, in March - May 2019. This study used a completely randomized design (CRD) with a factorial pattern of 3x3 with three replications. The treatment factors studied were (1) the type of planting media consisted of 3 levels namely alluvial soil, sand and husk charcoal, and (2) the type of growth regulator (ZPT) consisted of 3 levels namely control (without zpt), chemical (growtone) and organic (shallot extract). F test results on the analysis of variance showed that the planting medium had a very significant effect on the number of shoots 35 and 45 HST, number of leaves 15, 35 and 45 HST number of roots, root length, percentage of live cuttings. But the planting media had no significant effect on the number of shoots of 15 DAP. Growth regulator substances have a very significant effect on the number of shoots 35 HST, number of leaves 15, 35 and 45 HST, number of roots and root length. Significantly affect the number of shoots 45 HST and percentage of live cuttings, but no significant effect on the number of shoots 15 HST. This shows that the difference in growth of jasmine plant cuttings due to different planting media does not depend on growth regulators, and vice versa.


2020 ◽  
Vol 20 (2) ◽  
pp. 158-169
Author(s):  
Teguh Supriyadi ◽  
Tyas Soemarah K.D ◽  
Endang Suprapti ◽  
Agus Budiyono

The research entitled "The Effect of Concentration and Soaking Time of Pepper Cutters (Piper nigrum) in a Solution of Growth Regulatory Substances (auxin)" was carried out. Research was held on April – July 2020 in Green House of Agriculture Faculty, Tunas Pembangunan University, Jl. Balekambang Lor No. 1 Manahan, Surakarta. This study used a factorial experimental method based on a completely randomized design (CRD) consisting of 2 factors as treatment and 3 replications. The first factor, the concentration of growth regulators (auxin) (K), is divided into 3 levels, namely: 100 ppm (K1), 200 ppm (K2) and 300 ppm (K3); while the second factor, immersion time (P), also consists of 4 levels, namely: 2 hours (P1), 2.5 hours (P2), 3 hours (P3) and 3.5 hours (P4). The parameters observed in this study amounted to 9, with details as follows: Number of shoots, number of leaves, length of shoots, fresh and dry weight of shoots, number and length of roots, fresh weight and root dry weight. Based on the results of research on pepper cuttings due to the concentration of growth regulators (auxin) and the duration of immersion, the results showed that: Soaking time had a significant effect on the observed parameters, namely plant length in P2 treatment (2.5 hours). And it did not affect the parameters of the number of shoots, number of leaves, fresh and dry weight of shoots, number and length of roots, fresh weight and root dry weight.


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