scholarly journals Effect of Ethanol Extracts of Musa paradisiaca Fruit Pulp and Peels on Haematological Indices and Liver Enzymes of Experimental Rats

2021 ◽  
Vol 10 (1) ◽  
pp. 67-71
Author(s):  
Emuesiri Goodies Moke ◽  
Emuesiri Kohworho Umukoro ◽  
Evelyn Tarela Ojugbeli ◽  
Theresa Ezedom ◽  
Tarela Melish Elias Daubry ◽  
...  
Keyword(s):  
Liver Enzymes ◽  
Liver Toxicity ◽  
Haemoglobin Concentration ◽  
Experimental Period ◽  
Haematocrit Level ◽  
Fruit Pulp ◽  
Musa Paradisiaca ◽  
Red Cell Count ◽  
Ethanol Extracts ◽  
Haematological Indices

Medicinal plants usage in traditional medicine has risen over the years. Musa paradisiaca has been reported that it possesses various therapeutic efficacies. This study is aimed at evaluating the effect of parts of the ethanol fruit extracts of Musa paradisiaca on haematological indices and serum liver enzymes. Thirty Wistar rats were grouped into five groups of six animals each which were administered 200 mg/kg and 400 mg/kg of Musa paradisiac fruit (MPF) pulp extract or peel extract. Haematological indices and liver enzymes were assayed for at the end of the 14-days experimental period. MPF pulp and MPF peel at 400 mg/kg showed a significant (P<0.05) increase in red cell count and haematocrit level as compared to the control. There was a non-significant (P>0.05) increase in haemoglobin concentration in the treated rats as compared to the control. There was also a non-significant (P>0.05) change in AST, ALT, and ALP level of the treated rats as compared to the control. Fruit pulp and peel of Musa paradisiaca improve erythrocytes count and haematocrit level, and they may not be associated with liver toxicity.

Hepatoprotective effect of methanol fruit pulp extract of Musa paradisiaca on carbon tetrachloride-induced liver toxicity in Wistar rats

2018 ◽  
Vol 17 (1) ◽  
pp. 1
Author(s):  
AbelNosereme Agbon ◽  
MustaphaTosin Issa ◽  
SadiyaUfeli Balogun ◽  
Onesimus Mahdi ◽  
KhadijatAbubakar Bobbo ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Wistar Rats ◽  
Liver Toxicity ◽  
Hepatoprotective Effect ◽  
Fruit Pulp ◽  
Musa Paradisiaca

scholarly journals IN-VITRO ANTIOXIDANT AND IN-VIVO HEPATO PROTECTIVE ACTIVITY OF ETHANOL EXTRACTS FROM THE BARK OF SHOREA ROBUSTA (DIPTEROCARPACEAE) AGAINST CARBON TETRA CHLORIDE INDUCED LIVER TOXICITY IN RATS

2016 ◽  
Vol 9 (6) ◽  
pp. 225
Author(s):  
Diptanu Biswas
Keyword(s):  
Liver Toxicity ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Protective Effects ◽  
Shorea Robusta ◽  
Carbon Tetra ◽  
Anti Oxidant ◽  
Ethanol Extracts

ABSTRACT: The study is designed for the evaluation of in-vivo Hepato protective and in-vitro Anti oxidant activity of ethanol extracts from the bark of Shorea robusta (Dipterocarpaceae) by CCl4 induced hepatotoxicity in rats. Ethanol extracts from the bark Shorea robusta (EESR) was evaluated for hepatoprotective activity in rats by inducing liver damage by CCl4. The anti oxidant activity of EESR was assayed by various in-vitro antioxidant methods and activities were compared to standard ascorbic acid. Ethanol extracts at an oral dose 200mg/kg and 400mg/kg exhibited a significant (*p<0.005) protective effects by lowering the level of SGOT, SGPT, ALP, Serum bilirubin, total cholesterol and increasing the level of total proteins as compared to Silymarin (50mg/kg) used as positive control. The extracts exhibit significant anti oxidant activity in various in vitro anti oxidant models.  From these studies we are concluding that, the ethanolic extracts of S.robusta have potent hepatoprotective effects and have anti oxidant properties, hence can be used as a natural product against liver damage.KEY WORDS: Anti oxidant, Carbon tetra chloride,  Hepatoprotective,  Shorea robusta

scholarly journals Dietary vitamin D supplementation improves haematological status following consumption of an iron-fortified cereal: an 8-week randomised controlled trial

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Sohail Mushtaq ◽  
Salma F. Ahmed Fuzi
Keyword(s):  
Vitamin D ◽  
Placebo Group ◽  
Haemoglobin Concentration ◽  
Vitamin D Supplementation ◽  
Dietary Vitamin ◽  
Haematocrit Level ◽  
Post Intervention ◽  
Ferritin Concentration ◽  
Iron Deficient ◽  
Fortified Cereal

AbstractVitamin D, a secosteroid, has recently been implicated in the stimulation of erythroid precursors and ultimately the rate of erythropoiesis. However, there are a paucity of randomised controlled trials (RCT), investigating the effect of vitamin D supplementation iron status, especially in populations at risk of iron deficiency. An eight-week, double-blind RCT was carried out in 50 female (mean age (± SD): 27 ± 9 years), iron-deficient (plasma ferritin concentration < 20 μg/L) participants, randomised to consume an iron-fortified cereal containing 9 mg of iron, with either a vitamin D supplement (1,500 international units (IU)/day, 38 μg/day) or placebo. The effect of dietary vitamin D supplementation on haematological indicators was investigated. Blood samples were collected at baseline, 4-weeks and 8-week timepoints for measurement of iron and vitamin D status biomarkers. The effect of intervention was analysed with a mixed-model repeated measures ANOVA using IBM SPSS statistical software (Version 21, IBM Corporation, New York, USA). Significant increases were observed in two haematological parameters: haemoglobin concentration and haematocrit level from baseline to post-intervention in the vitamin D group, but not in the placebo group. The increase from baseline to post-intervention in haemoglobin concentration in the vitamin D group (135 ± 11 to 138 ± 10 g/L) was significantly higher than in the placebo group (131 ± 15 to 128 ± 13 g/L) (P ≤ 0.05). The increase in haematocrit level from baseline to post-intervention was also significantly higher in the vitamin D group (42.0 ± 3.0 to 43.8 49 ± 3.4%) compared to the placebo group (41.2 ± 4.3 to 40.7 ± 3.6%) (P ≤ 0.05). Despite non-significant changes in plasma ferritin concentration, this study demonstrates that dietary supplementation with 1,500IU vitamin D, consumed daily with an iron-fortified cereal led to improvement in haemoglobin concentration and haematocrit levels in women with low iron stores. Further long-term studies are required, however, these findings suggest a potential role for improvement of vitamin D status as an adjunct therapy for recovery of iron status in iron-deficient populations.

TLC‐bioautography identification and GC‐MS analysis of antimicrobial and antioxidant active compounds in Musa × paradisiaca L. fruit pulp essential oil

2019 ◽  
Vol 30 (3) ◽  
pp. 332-345 ◽  
Author(s):  
Mohd. Fahim ◽  
Mohammad Ibrahim ◽  
Sultan Zahiruddin ◽  
Rabea Parveen ◽  
Washim Khan ◽  
...  
Keyword(s):  
Essential Oil ◽  
Fruit Pulp ◽  
Active Compounds ◽  
Musa Paradisiaca ◽  
Tlc Bioautography ◽  
Ms Analysis

For how long should antibiotics be given in acute paediatric septic arthritis? A prospective audit of 96 cases

2007 ◽  
Vol 37 (4) ◽  
pp. 195-197 ◽  
Author(s):  
C B D Lavy ◽  
M Thyoka
Keyword(s):  
Septic Arthritis ◽  
Cell Count ◽  
Sedimentation Rate ◽  
White Cell Count ◽  
Haemoglobin Concentration ◽  
Prospective Audit ◽  
Erythrocyte Sedimentation ◽  
Radiological Changes ◽  
Haematological Indices ◽  
Infective Activity

Ninety-six cases of uncomplicated acute septic arthritis in children aged 12 years and under were treated by arthrotomy under general anaesthesia, saline washout and antibiotics for six weeks. They were prospectively studied for 24 weeks to assess clinical, haematological and radiological changes. Clinical improvement was most marked in the first two weeks and did not change significantly after six weeks. Haematological indices (haemoglobin concentration, serum white cell count and erythrocyte sedimentation rate) all improved from the start of treatment and continued to improve throughout the study, even after antibiotics were finished. Radiological changes in the bone adjacent to the infected joint were noted to be present in 21 cases by two weeks after presentation, and in a further 10 cases by six weeks after presentation, suggesting some continued infective activity in the bone adjacent to the septic joint even after two weeks of antibiotics. No new radiological changes were noted after six weeks. It is therefore suggested that antibiotics in septic arthritis should be continued for six weeks.

scholarly journals Effect of iron supplementation on mild to moderate anaemia in pulmonary tuberculosis

2003 ◽  
Vol 90 (3) ◽  
pp. 541-550 ◽  
Author(s):  
Uma Devi ◽  
C. Mohan Rao ◽  
Vinod K. Srivastava ◽  
Pramod K. Rath ◽  
Bhabani S. Das
Keyword(s):  
Placebo Group ◽  
Pulmonary Tuberculosis ◽  
Binding Capacity ◽  
Haemoglobin Concentration ◽  
Disease Process ◽  
Fe Deficiency ◽  
Adult Males ◽  
Blood Haemoglobin ◽  
Haematological Indices ◽  
Moderate Anaemia

Anaemia is a common complication of pulmonary tuberculosis. The precise mechanism of anaemia in pulmonary tuberculosis is not clearly known, but anaemia of inflammation as well as of Fe deficiency has been implicated. Both are common in developing countries. It is extremely difficult to distinguish anaemia of Fe deficiency from anaemia of inflammation with the haematological indices used routinely. Therefore, Fe preparations are usually prescribed for all anaemic patients irrespective of the aetiology. This approach has been questioned. The present study aimed to assess the effect of Fe supplementation on anaemic patients with pulmonary tuberculosis. Adult male patients 15–60 years of age with pulmonary tuberculosis and a blood haemoglobin concentration 80–110 g/l were included in the study; healthy adult males matched for age and socio-economic status were taken as controls. Blood haemoglobin concentration, total erythrocyte count (TEC), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin and serum Fe, total Fe-binding capacity and ferritin were estimated before treatment and 1, 2 and 6 months after treatment. The patients were divided randomly into three groups and during the initial 2 months of treatment were provided with one of three supplementary regimens consisting of placebo, Fe alone or Fe with other haematinics. Significant improvements in haematological indices and Fe status were noticed in all three groups. Blood haemoglobin concentration, MCV and PCV were significantly higher at 1 month in both Fe-supplemented groups than the placebo group. This difference, however, disappeared at 2 and 6 months with similar values in all three groups. The increase of other haematological indices was similar in all groups. Serum Fe and Fe saturation of transferrin were significantly higher in both Fe-supplemented groups than the placebo group up to 2 months; this effect, however, disappeared at 6 months. There was a consistent increase in TEC and decrease in ferritin values up to 6 months in all groups. Radiological and clinical improvement was similar in all three groups. These observations suggest that Fe supplementation in mild to moderate anaemia associated with pulmonary tuberculosis accelerated the normal resumption of haematopoiesis in the initial phases by increasing Fe saturation of transferrin. However, consistent improvement of haematological status was dependent only on the improvement of the disease process.

Genetic and Environmental Effects on Blood Cells

1993 ◽  
Vol 42 (3-4) ◽  
pp. 245-252 ◽  
Author(s):  
G.M.D. Dal Colletto ◽  
D.W. Fulker ◽  
O.C. de O. Barretto ◽  
M. Kolya
Keyword(s):  
Cell Count ◽  
Cell Volume ◽  
White Cell Count ◽  
Haemoglobin Concentration ◽  
Red Cell ◽  
Heritable Variation ◽  
Mean Cell Volume ◽  
Twin Analysis ◽  
Sources Of Variation ◽  
Red Cell Count

AbstractIn a sample of 105 concordant sex MZ and DZ twin pairs, the following characteristics were measured: red cell count, haemoglobin concentration, package cell volume, mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, reticulocytes, platelets, white cell count and the six types of leucocytes, lymphocytes, monocytes, band and segmented neutrophils, eosinophils and basophils. The statistical model employed in the univariate twin analysis allows for three sources of variation: genetic (h2), shared environmental (c2) and specific environmental influences (e2). A genetic component was significant for red cell count, haemoglobin and mean cell haemoglobin (0.64, 0.60 and 0.46 respectively), with heritable variation suggested for package cell volume, mean cell volume, mean cell haemoglobin, lymphocytes and monocytes. Shared environmental variation was only present for neutrophils.

scholarly journals Haematology and serum biochemistry of rabbits fed acha offal (Digitaria exilis) meal-based diets supplemented with Maxigrains® enzyme

2021 ◽  
Vol 48 (1) ◽  
pp. 102-113
Author(s):  
J. A. Azi ◽  
S. E. Alu ◽  
D. M. Ogah ◽  
M. K. Baba
Keyword(s):  
Biochemical Parameters ◽  
Crude Protein ◽  
Management Practices ◽  
Interactive Effect ◽  
Haemoglobin Concentration ◽  
Live Weight ◽  
Experimental Period ◽  
Serum Biochemistry ◽  
Randomized Design ◽  
Serum Biochemical

Food-feed competition between human and animals has necessitated farmers to turn to alternative feed resources such as Acha Offal. Forty eight rabbits of mixed breeds and similar live weight were utilized in an experiment to evaluate the effect of feeding acha offal meal- based diets supplemented with Maxigrain enzyme on their haematology and serum biochemistry. The experiment had two phases (weaner and grower) each lasting for 42 days. Six treatments diets namely T1, T2, T3, T4, T5 and T6 were compounded to be iso-caloric (2700kcal/kg) and iso-nitrogenous (18% crude protein) for the weaner phase while (15% crude protein) and isocaloric (2500kcal/kg) for the grower phase. The Acha Offal (AO) was included at 0, 15 and 30% while the enzyme was supplemented at 0 and 200ppm. The experiment was arranged in a 2x3 factorial fitted into a Completely Randomized Design. Each treatment was replicated 4 times having two rabbits per replicate. Feed and water were provided to the animals daily and all standard routine management practices were strictly observed throughout the experimental period. At the end of the feeding trial, blood samples were collected from each animal for blood analyses. The results show that there was no significant (P>0.05) variation on the haematological and biochemical parameters due to enzyme or AO effect. In the growing phase, main effect of AO was not significant (P>0.05) on the haematological parameters except on white blood cell (WBC) and mean cell haemoglobin concentration (MCHC). Effect of enzymes on growing rabbit was not significantly different (P>0.05) except on WBC at 0ppm (5.75 x109/L) and 200ppm (6.60 x109/L) and on MCHC at 0ppm (33.27 x109/L) and 200ppm (33.20 x109/L). The Interactive effect of AO and enzyme showed that T2 had the highest WBC (8.80 x109/L) while T4 had the lowest MCHC (33.10 x109/L). Serum biochemical parameters were not affected by Acha offal except phosphorus where there was a significant (P<0.05) reduction in 30% AO (1.35g/dL). Enzymes effect showed that animals on 0ppm had significantly (P<0.05) higher phosphorus (1.83g/dl) than 200ppm (1.38g/dL). Acha offal supplemented with Maxigrain enzyme can be included in the diets of grower rabbits without adverse effect on haematological and serum biochemical indices.     La concurrence entre les humains et les animaux en matière d'aliments pour animaux a obligé les agriculteurs à se tourner vers d'autres ressources alimentaires comme 'acha'. Quarante-huit lapins de races mixtes et de poids réel similaire ont été utilisés dans une expérience pour évaluer l'effet de l'alimentation d'acha complétés par l'enzyme 'Maxigrain' sur leur hématologie et biochimie sérique. L'expérience a eu deux phases (sevreur et producteur) chacune durant 42 jours. Six régimes de traitements, à savoir T1, T2, T3, T4, T5 et T6, ont été composés pour être iso-caloriques (2700kcal/kg) et iso-azotés (18% de protéines brutes) pour la phase de sevrage tandis que (15% de protéines brutes) et isocaloriques (2500 kcal/kg) pour la phase de croissance. L'alimentation d'acha (AO) a été inclus à 0, 15 et 30% tandis que l'enzyme a été complétée à 0 et 200ppm. L'expérience a été organisée dans un factorial 2x3 intégré dans une conception complètement randomisée. Chaque traitement a été reproduit 4 fois ayant deux lapins par réplique. Des aliments et de l'eau ont été fournis quotidiennement aux animaux et toutes les pratiques de gestion de routine standard ont été strictement observées tout au long de la période expérimentale. À la fin de l'essai d'alimentation, des échantillons de sang ont été prélevés sur chaque animal pour des analyses sanguines. Les résultats montrent qu'il n'y avait aucune variation significative (P>0.05) sur les paramètres hématologiques et biochimiques dus à l'effet d'enzyme ou d' 'AO'. Dans la phase de croissance, l'effet principal de l' 'AO' n'était pas significatif (P>0,05) sur les paramètres hématologiques, sauf sur les globules blancs (le 'WBC') et la concentration moyenne d'hémoglobine cellulaire (le 'MCHC'). L'effet des enzymes sur la croissance du lapin n'était pas significativement différent (P>0,05) excepté sur WBC à 0ppm (5.75 x109/L) et 200ppm (6.0ppm 60 x109/L) et sur le 'MCHC' à 0ppm (33.27 x109/L) et 200ppm (33.20 x109/L). L'effet interactif de l'AO et de l'enzyme a montré que T2 avait le 'WBC' le plus élevé (8,80 x109/L) tandis que T4 avait le 'MCHC' le plus bas (33,10 x109/L). Les paramètres biochimiques du sérum n'ont pas été affectés par le phosphore d'alimentation d'acha sauf où il y avait une réduction significative (P<0.05) de 30%AO (1.35g/dL). L'effet d'enzymes a prouvé que les animaux sur 0ppm ont eu sensiblement (P<0.05) le phosphore plus élevé (1.83g/dl) que 200ppm (1.38g/dL). L'alimentation d'Acha complété avec l'enzyme 'Maxigrain' peut être inclus dans les régimes des lapins 'grower' sans effet défavorable sur les indices biochimiques hématologiques et sériques.

scholarly journals The effects of low levels of aflatoxin B1 on health, growth performance and reproductivity in male rabbits

2018 ◽  
Vol 26 (2) ◽  
pp. 123 ◽  
Author(s):  
Yawang Sun ◽  
Guozhong Dong ◽  
Guangxin E ◽  
Min Liao ◽  
Lin Tao ◽  
...  
Keyword(s):  
Liver Function ◽  
Immune Function ◽  
Growth Performance ◽  
Aflatoxin B1 ◽  
Semen Quality ◽  
Block Design ◽  
Average Daily Gain ◽  
Haemoglobin Concentration ◽  
Testis Weight ◽  
Haematological Indices

This study investigated the chronic effects of relatively low exposure to aflatoxin B1 (AFB1) on the growth performance, immune situation and reproduction in male rabbits. Bucks (n=32, 4.82±0.22 kg) were individually assigned to 4 treatments (8 replicates each) using a randomised complete block design. Four diets containing 0, 0.02, 0.05, and 0.1 mg AFB1/kg, were provided to bucks for 8 wk. Growth performance and semen quality were measured. Blood, organ and tissue samples were collected to measure haematological indices, liver function, organ weights and immune parameters. Compared to control, AFB1-contaminated diets reduced body weight and average daily gain (<em>P</em>&lt;0.05), altered certain haematological indices and liver function with decreased monocytes percentage and mean corpuscular haemoglobin concentration, and increased plateletcrit and albumin (<em>P</em>&lt;0.05), slightly impaired reproductive parameters with enhanced ratio of morphologically abnormal sperm cells at early stage and reduced post-stage acrosome integrity, testis weight and serum testosterone concentration (<em>P</em>&lt;0.05), decreased immune function with reduced relative liver weight (%) and tumour necrosis factor-α levels in serum and liver tissue, and increased serum 8-hydroxy-2’-deoxyguanosine levels (<em>P</em>&lt;0.05). Furthermore, bucks fed diets with relatively high AFB1 (0.05 and 0.1 mg AFB1/kg) had reduced red blood cell and haematocrit (<em>P</em>&lt;0.05) in contrast with the low AFB1 group (0.02 mg AFB1/kg). In conclusion, diets containing 0.05 and 0.1 mg AFB1/kg had negative effects on bucks’ growth performance, haematology, reproductivity and immune function, whereas diet containing 0.02 mg AFB1/kg had only minor effects on the parameters measured.

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