scholarly journals Haematology and serum biochemistry of rabbits fed acha offal (Digitaria exilis) meal-based diets supplemented with Maxigrains® enzyme

2021 ◽  
Vol 48 (1) ◽  
pp. 102-113
Author(s):  
J. A. Azi ◽  
S. E. Alu ◽  
D. M. Ogah ◽  
M. K. Baba
Keyword(s):  
Biochemical Parameters ◽  
Crude Protein ◽  
Management Practices ◽  
Interactive Effect ◽  
Haemoglobin Concentration ◽  
Live Weight ◽  
Experimental Period ◽  
Serum Biochemistry ◽  
Randomized Design ◽  
Serum Biochemical

Food-feed competition between human and animals has necessitated farmers to turn to alternative feed resources such as Acha Offal. Forty eight rabbits of mixed breeds and similar live weight were utilized in an experiment to evaluate the effect of feeding acha offal meal- based diets supplemented with Maxigrain enzyme on their haematology and serum biochemistry. The experiment had two phases (weaner and grower) each lasting for 42 days. Six treatments diets namely T1, T2, T3, T4, T5 and T6 were compounded to be iso-caloric (2700kcal/kg) and iso-nitrogenous (18% crude protein) for the weaner phase while (15% crude protein) and isocaloric (2500kcal/kg) for the grower phase. The Acha Offal (AO) was included at 0, 15 and 30% while the enzyme was supplemented at 0 and 200ppm. The experiment was arranged in a 2x3 factorial fitted into a Completely Randomized Design. Each treatment was replicated 4 times having two rabbits per replicate. Feed and water were provided to the animals daily and all standard routine management practices were strictly observed throughout the experimental period. At the end of the feeding trial, blood samples were collected from each animal for blood analyses. The results show that there was no significant (P>0.05) variation on the haematological and biochemical parameters due to enzyme or AO effect. In the growing phase, main effect of AO was not significant (P>0.05) on the haematological parameters except on white blood cell (WBC) and mean cell haemoglobin concentration (MCHC). Effect of enzymes on growing rabbit was not significantly different (P>0.05) except on WBC at 0ppm (5.75 x109/L) and 200ppm (6.60 x109/L) and on MCHC at 0ppm (33.27 x109/L) and 200ppm (33.20 x109/L). The Interactive effect of AO and enzyme showed that T2 had the highest WBC (8.80 x109/L) while T4 had the lowest MCHC (33.10 x109/L). Serum biochemical parameters were not affected by Acha offal except phosphorus where there was a significant (P<0.05) reduction in 30% AO (1.35g/dL). Enzymes effect showed that animals on 0ppm had significantly (P<0.05) higher phosphorus (1.83g/dl) than 200ppm (1.38g/dL). Acha offal supplemented with Maxigrain enzyme can be included in the diets of grower rabbits without adverse effect on haematological and serum biochemical indices.     La concurrence entre les humains et les animaux en matière d'aliments pour animaux a obligé les agriculteurs à se tourner vers d'autres ressources alimentaires comme 'acha'. Quarante-huit lapins de races mixtes et de poids réel similaire ont été utilisés dans une expérience pour évaluer l'effet de l'alimentation d'acha complétés par l'enzyme 'Maxigrain' sur leur hématologie et biochimie sérique. L'expérience a eu deux phases (sevreur et producteur) chacune durant 42 jours. Six régimes de traitements, à savoir T1, T2, T3, T4, T5 et T6, ont été composés pour être iso-caloriques (2700kcal/kg) et iso-azotés (18% de protéines brutes) pour la phase de sevrage tandis que (15% de protéines brutes) et isocaloriques (2500 kcal/kg) pour la phase de croissance. L'alimentation d'acha (AO) a été inclus à 0, 15 et 30% tandis que l'enzyme a été complétée à 0 et 200ppm. L'expérience a été organisée dans un factorial 2x3 intégré dans une conception complètement randomisée. Chaque traitement a été reproduit 4 fois ayant deux lapins par réplique. Des aliments et de l'eau ont été fournis quotidiennement aux animaux et toutes les pratiques de gestion de routine standard ont été strictement observées tout au long de la période expérimentale. À la fin de l'essai d'alimentation, des échantillons de sang ont été prélevés sur chaque animal pour des analyses sanguines. Les résultats montrent qu'il n'y avait aucune variation significative (P>0.05) sur les paramètres hématologiques et biochimiques dus à l'effet d'enzyme ou d' 'AO'. Dans la phase de croissance, l'effet principal de l' 'AO' n'était pas significatif (P>0,05) sur les paramètres hématologiques, sauf sur les globules blancs (le 'WBC') et la concentration moyenne d'hémoglobine cellulaire (le 'MCHC'). L'effet des enzymes sur la croissance du lapin n'était pas significativement différent (P>0,05) excepté sur WBC à 0ppm (5.75 x109/L) et 200ppm (6.0ppm 60 x109/L) et sur le 'MCHC' à 0ppm (33.27 x109/L) et 200ppm (33.20 x109/L). L'effet interactif de l'AO et de l'enzyme a montré que T2 avait le 'WBC' le plus élevé (8,80 x109/L) tandis que T4 avait le 'MCHC' le plus bas (33,10 x109/L). Les paramètres biochimiques du sérum n'ont pas été affectés par le phosphore d'alimentation d'acha sauf où il y avait une réduction significative (P<0.05) de 30%AO (1.35g/dL). L'effet d'enzymes a prouvé que les animaux sur 0ppm ont eu sensiblement (P<0.05) le phosphore plus élevé (1.83g/dl) que 200ppm (1.38g/dL). L'alimentation d'Acha complété avec l'enzyme 'Maxigrain' peut être inclus dans les régimes des lapins 'grower' sans effet défavorable sur les indices biochimiques hématologiques et sériques.

scholarly journals Haematological and Serum Biochemical Profile of Growing Yankasa Ram Lambs Fed Diets Containing Graded Levels of Sesame Residue

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
Y. Garba ◽  
E. A. Adeola
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Serum Biochemical Parameters ◽  
Corpuscular Haemoglobin ◽  
Randomized Design ◽  
Serum Biochemical ◽  
Mean Corpuscular Haemoglobin ◽  
Ram Lambs

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.

Haematological and serum biochemical indices of growing rabbits fed graded levels of Yam peel meal as replacement for maize

2020 ◽  
Vol 47 (4) ◽  
pp. 167-175
Author(s):  
I. B. Amaza ◽  
A. Maidala ◽  
C. E. Isidahomen
Keyword(s):  
Biochemical Analysis ◽  
Experimental Period ◽  
Blood Chemistry ◽  
Feeding Trial ◽  
Randomized Design ◽  
Serum Biochemical ◽  
Inclusion Level ◽  
Completely Randomized Design ◽  
Biochemical Indices ◽  
Dietary Treatments

Haematological and serum biochemical parameters are indicators of physiological, nutritional and pathological status of animals. Investigation of these parameters provide information for diagnostic purposes. The study was conducted to determine the influence of feeding graded levels of yam peel meal (YPM) as a replacement for maize on haematology; blood chemistry was evaluated in rabbits. Thirty- six (New Zealand white X Chinchilla) were randomly assigned to four dietary treatments in a completely randomized design with nine rabbits per treatment. The rabbits were fed diets containing 0, 12.5, 25 and 37.5% of Yam peel meal (YPM) as a replacement for maize, designated as T1, T2, T3 and T4, respectively. The experimental diets and clean drinking water were supplied ad libitum throughout the experimental period of nine weeks. At the end of the feeding trial, three rabbits per treatment were randomly selected for hematological and serum biochemical analysis. All the haematological and serum biochemical indices except AST were affected (P<0.05) by dietary inclusion of YPM. Although most of the parameters investigated were affected by rabbits fed test ingredient, the values fell within the normal literature reference values for rabbits. It can therefore be concluded that up to 37.5% inclusion level of YPM can be fed to growing rabbits without deleterious effects on health status.

scholarly journals Effect of Low Level Acute of Aflatoxin on Performance in Faw- Bro Broiler

2008 ◽  
Vol 5 (3) ◽  
pp. 357-361
Author(s):  
Baghdad Science Journal
Keyword(s):  
White Blood Cells ◽  
Relative Weight ◽  
Live Weight ◽  
Serum Biochemistry ◽  
Feed Conversion ◽  
Broiler Breeder ◽  
Volume Percentage ◽  
Low Level ◽  
Randomized Design ◽  
Long Time

This study was conducted in the Poultry farm of the animal during the Production department, Iraqi during the (Ministry of Science and Technology) period from 3-9-2001 to 8-4-2002. The objectives of this study were to evaluate the effect of low – level chronic aflatoxicosis on performance (body weight, feed conversion efficiency and mortality), Serum biochemistry and activity of some enzymes (GOT,GPT, ALKP, LDH). A total of 300 male chicks of broiler breeder (Faw–Bro) were used. Chicks at day 1 of age were fed diets contaminated with aflatoxine at levels of 0, 0.3, 0.6, 0.9, 1.2, and 1.5 the feeding period were extended to 8 weeks. The data were subjected to analysis of variance by the completely randomized design. The results showed the presence of a number of fungi especially the type of Aspergillus flavus genus that reaches 40%. All aflatoxine dose levels significantly (P < 0.05) decreased live weight, it was also noticed that there were higher significant decrease in feed conversion when aflatoxine level was elevated in the feed. Increasing mortality with each increasing in aflatoxine level, the low level of chronic aflatoxine in the diets caused increased in the relative weight of internal organs (Liver, Pancreases, spleen, heart, gizzard, fibresia gland) as a results of aflatoxine presence in the diet, the low level of chronic aflatoxicosis for long time decreased in packed cell volume percentage, heamoglbulin concentration and red blood cell cont., with increase in white blood cells and (neutrophil, Hetrophil, Lymphocyte). The aflatoxine increased the serum concentration of protein, albumin, Cholesterol, Phosphor the activity of LDH, GOT, GPT, AIKP.

scholarly journals Age – Related changes in serum biochemical parameters in the domestic rabbit (Oryctolagus cuniculus)

2020 ◽  
Vol 45 (5) ◽  
pp. 67-71
Author(s):  
K. A. Adebisi
Keyword(s):  
Total Protein ◽  
Biochemical Parameters ◽  
Serum Biochemistry ◽  
Serum Biochemical Parameters ◽  
Serum Total Protein ◽  
Domestic Rabbit ◽  
Age Related ◽  
Protein 4.2 ◽  
Pubertal Stages ◽  
Serum Biochemical

The influence of age on serum biochemical components in the domestic rabbit was investigated. The serum total protein (g/dL), albumin (g/dL), globulin (g/dL), creatinine (mg/dL), urea mg/dL, cholesterol (mg/dL), Alkaline phosphatase (ALP; iu/l), Aspartate aminotranferase (AST; iu/l) and Alanine amimotransferase (ALT; iu/l) were assessed in 24 male rabbits at 7, 14 and 21 weeks of age to represent weaners, growers and pubertal stages. Data were analysed using descriptive statistics and analysis of variance at á . Results of 0.05 serum biochemistry revealed a steady significant increase in total protein (4.2±0.3; 5.3±0.5; 6.9±0.2), albumin (2.2±0.7; 2.8±0.4; 4.0±0.2), globulin (1.9±0.3; 2.5±0.4, 2.9±0.2), cholesterol (49.5±1.1; 56.4±4.2; 62.4±5.4) and creatinine (0.8±0.1; 1.2±0.1; 1.9±0.3) at 7, 14 and 21 weeks respectively. Serum urea levels were similar across the ages. The values recorded for ALP and AST at 7 weeks (39.6±7.5; 101.1±6.7) and 14 weeks (38.7±6.4; 104.5±8.7) were significantly higher than the 21 weeks (33.5±2.7; 95.8±5.9) while ALT at 7 weeks (27.33±5.6) was significantly lower than 14 (38.2±3.9) and 21 weeks (37.4±3.0). The age of the rabbits had a significant effect on their serum biochemical parameters. They should therefore be grouped according to age when setting serum reference values for them.

Effects of Dietary Soybean (Glycine max) on Growth Performance, Haematology and Serum Biochemistry of African Carp (Labeo Cubie Ruppell 1832)

2021 ◽  
Vol 3 (1) ◽  
pp. 72-90
Author(s):  
B. T Adeyemo
Keyword(s):  
Growth Performance ◽  
Soybean Meal ◽  
Fish Meal ◽  
Haemoglobin Concentration ◽  
Serum Biochemistry ◽  
Uric Acid Concentration ◽  
Performance Indices ◽  
Serum Biochemical ◽  
African Carp ◽  
Apparently Healthy

The effects of dietary replacements of fish meal with soybean meal on growth performance, heamatology and serum biochemistry in juvenile Labeo cubie were evaluated. Four hundred and fifty (450) apparently healthy juvenile Laboe cubie were distributed into one of fifteen 1000 L tanks divided into five groups and fed any of diets A (0% soybean meal); B (30% soybean meal); C (40% soybean meal); D (45% soybean meal) and diet E (75% soybean meal) for 56 days. At set time points day 7, 14, 28 and day 56, five fish were randomly selected per tank for growth assessments, heamatology and serum biochemical evaluations. Growth performance indices decreased significantly (p < 0.05) along the gradient of soybean meal inclusion and the duration of feeding. The erythrocytes count increased significantly (p < 0.05) at day 14 in fish fed soybean meal diets and decreased significantly (p < 0.05) beginning at day 28 in fish fed soybean meal-based diets. The haematocrit of fish fed diets D and E were significantly (p < 0.05) lower at day 28 compared to day 56. There were no significant variations in haemoglobin concentration and the leucocytes counts (p > 0.05). The proteins, blood glucose and the transaminases increased significantly (p < 0.05) along gradient of dietary inclusion and duration of feeding in the first 28 days while creatinine kinase activity, blood urea nitrogen and uric acid concentration remained significantly elevated (p < 0.05) in fish fed the soybean meal-based diets. The results of the study show juvenile L. cubie may tolerate up to 40% dietary inclusion of soybean meal as replacement for fish meal.

Effect of graded levels of nucleotide supplementation on certain serum biochemical parameters in Japanese quails

2016 ◽  
Author(s):  
S. Prakash ◽  
Jyoti Palod ◽  
R. K. Sharma ◽  
S. K. Singh
Keyword(s):  
Biochemical Parameters ◽  
Protein Profile ◽  
Hdl Cholesterol ◽  
Serum Glucose ◽  
Serum Biochemical Parameters ◽  
Treatment Groups ◽  
Japanese Quails ◽  
Randomized Design ◽  
Serum Biochemical ◽  
G Ratio

A trial was conducted for a period of 6 weeks to evaluate the effect of graded levels of nucleotide supplementation on serum biochemical parameters in Japanese quails. In the trial, 3 day old Japanese quail chicks were randomly distributed in completely randomized design into 4 treatment groups each with 3 replicates of 10 Japanese quails. The Japanese quails of treatment groups T1, T2, T3 and T4 were provided feed containing 0, 0.5, 1.0 and 1.5 per cent nucleotide respectively for a period of six weeks. At the end of experiment blood samples were collected for study of serum-biochemical parameters. The results indicated that decreased serum glucose, serum cholesterol and LDL cholesterol and increased HDL cholesterol was noticed in nucleotide supplemented groups at higher levels. Protein profile showed significant improvement in total protein, albumin and globulin while A/G ratio revealed no effect of supplementation. Serum creatinine and SGPT contents showed non-significance whereas, serum uric acid and SGOT contents were significantly (P<0.05) reduced in nucleotide supplemented groups of quails. It is concluded that nucleotide supplementation improves serum biochemical parameters without affecting liver and kidney functions.

A study of protein requirements of housed Scottish Blackface ewes during late pregnancy

1971 ◽  
Vol 13 (4) ◽  
pp. 643-651 ◽  
Author(s):  
T. H. McClelland ◽  
T. J. Forbes
Keyword(s):  
Weight Gain ◽  
Protein Intake ◽  
Crude Protein ◽  
Nitrogen Retention ◽  
Live Weight ◽  
Late Pregnancy ◽  
Experimental Period ◽  
Apparent Digestibility ◽  
Live Weight Gain ◽  
Digestible Crude Protein

SUMMARYIn-lamb ewes were given 38, 58 and 82 g of digestible crude protein daily at a constant energy intake of 1600 kcal ME per head daily during the last 6 weeks of gestation. There were no significant differences between treatments in live-weight gain of the ewes, lamb birth weight or ewe net body-weight change (the difference between live-weight gain over the experimental period and live-weight loss at lambing).Digestibility coefficients for the diet components and nitrogen balances were determined on three ewes per treatment during weeks 15-16 and 19·20 of gestation. No differences in the apparent digestibility of the diet components were observed between the two periods. The apparent digestibility of crude protein increased significantly (P > 0·001) with increase in crude protein intake. A significant linear relationship (P<0·01) was found between digestible crude protein intake and nitrogen retention; daily N retention increased by 0·19 g per kg W0·73 for every 1 g per kg W0·73 of apparent digested nitrogen intake per day.

scholarly journals Effects of concentrate mixture level on nutrient digestibility, growth, production and reproduction performance of Jamunapari goat under semi intensive condition

2021 ◽  
Vol 8 (2) ◽  
pp. 223-230
Author(s):  
Nusrat Zahan Shoshe ◽  
Md Abdullah Al Mahmud ◽  
Piash Kumer Ghosh
Keyword(s):  
Body Weight ◽  
Crude Protein ◽  
Dry Matter ◽  
Live Weight ◽  
Nutrient Digestibility ◽  
Feed Digestibility ◽  
Randomized Design ◽  
Reproduction Performance ◽  
Concentrate Mixture ◽  
Growth Production

Twenty female Jamunapari goats of live weight of 10.5 ± 1.2 kg at 08± 0.56 months of age were used for 240 days feeding trial. Goats were assigned to four dietary treatments in a completely randomized design with five (05) goats in each group. All animals were supplied ad libitum green grass and water. They were supplied 150, 200, 250, 300 gm concentrate mixture per day with roughage for group T1, T2, T3, and T4 respectively. Total dry matter (TDM) intake (413.2, 456.67, 489.2 and 495.7g/day) in groups T1, T2, T3, and T4, respectively, was significantly (p≤0.01) influenced by the level of concentrate in the diet. Average total digestible crude protein (DCP) intake (3.1, 3.5, 3.9, 4.0 g/kg body weight and average total digestible nutrient (TDN) intake 21.1, 22.3, 24.7, 25.1 g/kg body weight was significantly (p≤0.01) higher in group T4 and lower in group T1. The nutrient intake and digestibility were also significantly higher (p≤0.01) in T4 group. The percent digestibility of dry matter (DM), organic matter (OM), crude protein (CP), crude fiber (CF), ether extract (EE) and nitrogen free extract (NFE) were 70.1, 75.99, 73.50, 67.24, 62.13 and 49.60%, 73.14, 76.97,76.84, 68.32, 64.04 and 53.25%, 75.45,78.44,78.67,70.43,68.43 and 59.77%, 79.56, 80.16,81.69,72.45,70.82 and 63.09% in T1, T2, T3 and T4 group, respectively. Reproductive performances, birth weight of kids and litter size did not differ significantly between groups, but had significant effect on feed digestibility and milk production. Daily 300 gm concentrate mixture along with roughage is necessary for better growth, production and reproduction performance of Jamunapari doe in semi-intensive system. Res. Agric., Livest. Fish.8(2): 223-230, August 2021

scholarly journals Effect of multienzymes + probiotic supplementation in fermented shea butter cake based diets on the carcass traits and the biochemistry of broiler chickens

2020 ◽  
Vol 12 (3) ◽  
pp. 227-233
Author(s):  
P.C. Aguihe ◽  
A.S. Kehinde ◽  
S.K. Halidu ◽  
A. Sulyman ◽  
J. Chikezie ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Live Weight ◽  
Serum Biochemistry ◽  
Positive Control ◽  
Serum Glucose ◽  
Negative Control ◽  
Shea Butter ◽  
Broiler Chicks ◽  
Breast Meat ◽  
Randomized Design

Abstract. This study aimed to evaluate the effect of multienzyme+probiotic (MEP) supplementation on carcass characteristics and serum biochemistry of broiler chickens fed fermented shea butter cake meal (FSBCM) based diets. A total of two hundred and sixty (260), 28-day-old broiler chicks were distributed into four treatments, with five replicates of 13 birds each using a completely randomized design and reared on deep litter system from 28 to 56 days of age. The four treatments were: positive control – T1 (corn-soybean based diet); negative control – T2 (20% FSBCM replacement for corn in T1); diet 3 – T3 (T2 diet + 0.05% MEP) and diet 4 – T4 (T2 diet + 0.10% MEP). On day 56, two birds per each pen were selected for carcass and organ evaluation and other two birds per replicate were selected for serum biochemical analysis. The results indicated that addition of MEP to FSBCM diet caused a significant (p<0.05) increase in live weight, dressing percentage and breast meat yield than FSBCM diet without MEP and did not differ from the T1 diet. The abdominal fat in birds fed MEP treated diets were significantly (p<0.05) lower than those groups fed PC and FSBCM diets. Serum glucose was similar (p>0.05) between birds fed PC and MEP treated diets but significantly (p<0.05) higher than those on FSBCM diet, whereas serum cholesterol, alanine aminotransferase and aspartate aminotransferase concentrations reduced significantly (p<0.05) in groups fed MEP supplemented FSBCM diets. All other parameters were not significantly (p>0.05) affected by the MEP supplementation. Therefore, MEP supplementation in FSBCM based diet improved live weights and carcass attributes without any detrimental effect on blood composition of the broiler chickens.

scholarly journals Changes in serum biochemistry of fish, Catla catla in response to metal toxicity

2020 ◽  
Vol 3 (1) ◽  
pp. 41-45
Author(s):  
Wardah Hassan ◽  
Sajid Abdullah ◽  
Naila Hadayat ◽  
Huma Naz ◽  
Shakeela Parveen
Keyword(s):  
Heavy Metals ◽  
Metal Toxicity ◽  
Biochemical Parameters ◽  
Serum Biochemistry ◽  
Catla Catla ◽  
Target Tissue ◽  
Serum Biochemical Parameters ◽  
Exposed Fish ◽  
Treated Fish ◽  
Serum Biochemical

In the present study an attempt has been made to evaluate the effect of heavy metals i.e. nickel (Ni) and zinc (Zn) on the serum biochemical parameters of Catla catla. The fish were exposed to lethal concentrations (96-hr LC50) of selected metals. The studied serum biochemical parameters were sodium (Na), potassium (K), chloride (Cl), albumin (Alb), urea (U), glucose (Glu), aspartate aminotransferase (Ast) and alanine aminotransferase (Alt). Results revealed a decrease in Na, Cl and Alb levels of metal exposed fish at all exposure periods as compared to control. However, an increase in K, U, Glu, Ast and Alt levels was observed in the metal exposed fish. All the biochemical parameters showed significant dependence on exposure durations in both treated fish except Na and Alb in Zn treated fish. This study indicated that the alterations in serum biochemical parameters may be the result of the target tissue damage and dysfunction induced by the metal and that these parameters can thus be used to assess the toxic effects of metals on organisms.

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