PHENOTYPES OF EOSINOPHILS IN THE DIAGNOSIS OF ATOPIC BRONCHIAL ASTHMA IN CHILDREN

The aim of the research was to study the phenotype of eosinophils in children with atopic bronchial asthma and their role as diagnostic criteria of the disease. Materials and methods. We have examined 130 children aged from 6 to 18 years old with atopic bronchial asthma. The control group consisted of 40 healthy children from 6 to 18 years old, who didn’t have allergic diseases. During the research the relative and absolute levels of eosinophils, the level of eosinophils carrying FcεRI and FcεRII (CD23) – receptors were established. Results. The relative level of eosinophils in asthmatic children was 6.00% [3.00; 8.00], the absolute level – 417.00 cells/μL [232.00; 636,00], which is significantly higher compared to control group, where the relative level was 2.50% [1.00; 3.00%] and the absolute level was 166.50 cells/μl [86.00; 213.00] (p<0.001). The level of eosinophils carrying the FcεRI receptor was 420,00 cells/μl [250,00; 660,00], which is significantly higher than in the control group – 200,00 cells/μl [140,00; 240.00] (p<0.001). The relative level of eosinophils carrying CD23 receptor on their surface was 62.20% [35.40; 76.60] and the absolute level –223.37 cells/μl [105.30; 375.24]. The results are significantly higher than those of the control group: 25.45% [14.30; 30,60] (p<0,001), and 30,88 cells/μl [25,63; 42.84] (p <0.0001) respectively. It was found in the ROC-analysis that there is high evidence of presence of atopic bronchial asthma if absolute levels of eosinophils carrying CD23 receptor was 73.008 cells/μl or more or the relative index – 35.10% and more. Conclusions. The relative and absolute levels of eosinophils, as well as eosinophils carrying FcεRI and CD23 receptors are statistically significantly higher in atopic bronchial asthma than in control group patients. Increased number or relative index (73.008 cells/μl or ≥ 35.10% respectively) of eosinophils carrying CD 23 receptors can be considered as a diagnostic criterion of atopic bronchial asthma.

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ОЦЕНКА ЦИТОКИНОВОГО ПРОФИЛЯ СЫВОРОТКИ КРОВИ И СУБПОПУЛЯЦИЙ Т-ЛИМФОЦИТОВ У ДЕТЕЙ С АЛЛЕРГИЧЕСКИМИ ЗАБОЛЕВАНИЯМИ ОРГАНОВ ДЫХАНИЯ

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/raj.2019.3.40502 ◽

2019 ◽

pp. 52-60

Author(s):

E.V. Prosekova ◽

A.I. Turyanskaya ◽

N.G. Plekhova ◽

M.S. Dolgopolov ◽

V.A. Sabynych

Keyword(s):

Allergic Rhinitis ◽

Bronchial Asthma ◽

Allergic Inflammation ◽

Allergic Diseases ◽

Control Group ◽

Healthy Children ◽

Serum Cytokine ◽

Immunological Parameters ◽

Low Level ◽

Level Of Significance

Расширение спектра изучаемых клонов Тхелперов определило более сложные иммунные механизмы реализации аллергического воспаления. Цель. Характеристика показателей и взаимосвязей цитокинового профиля сыворотки и субпопуляционного состава Тлимфоцитов периферической крови у детей с бронхиальной астмой и аллергическим ринитом. Материалы и методы. Проведено комплексное обследование 150 детей в возрасте 311 лет с верифицированным диагнозом бронхиальной астмы, аллергического ринита и 30 здоровых сверстников. Иммунологические параметры крови оценивали методом проточной цитометрии, концентрации интерлейкинов и IgE в сыворотке крови определяли методом твердофазного иммуноферментного анализа. При статистической обработке использовали программы Statistica 10 с критическим уровнем значимости р0,05. Результаты. У детей с аллергическими заболеваниями в сыворотке крови определены высокие уровни содержания интерлейкинов4, 8, 13, 17А, сопоставимый с показателями группы контроля уровень IL17F и низкое содержание IFNy. При бронхиальной астме и аллергическом рините у детей выявлено увеличение количества CD3CD8CD45RO, CD3CD8CD45RACD45RO Тлимфоцитов и CD3CD4 Тхелперов и повышение количество Th17 при снижении CD3CD4CD45RO клеток памяти. В группе здоровых детей популяция Th17 составляла 9,491,6, у детей с аллергическими заболеваниями количество данных клеток было значимо выше 14,50,77 (р0,001). Анализ сывороточного содержания цитокинов у детей с изолированным течением БА и в сочетании с аллергическим ринитом выявил разнонаправленные корреляции, отличающиеся по силе и направленности от таковых в группе здоровых детей. Заключение. У детей при изолированном течении бронхиальной астмы и в сочетании с аллергическим ринитом выявлены: сопоставимое с показателями здоровых детей количество CD3CD4 Тклеток, дисбаланс в субпопуляционном составе Тхелперов за счет преобладания Th2 и Th17, активация синтеза IL17A, IL4, IL8, IL13, низкий уровень сывороточного IFNy, изменения силы и направленности взаимосвязей цитокинового профиля и спектра субпопуляций Тлимфоцитов.Expansion of the range of examined Thelper clones has determined more complex immune mechanisms for the implementation of allergic inflammation. Objective. To characterize the parameters and relationships between the serum cytokine profile and Tlymphocyte subpopulation in peripheral blood of children with bronchial asthma and allergic rhinitis. Materials and methods. 150 children aged between 311 years old with bronchial asthma, and allergic rhinitis and 30 healthy volunteers were examined. Immunological parameters were assessed by flow cytometry, the concentration of serum interleukins and IgE were determined by means of enzymelinked immunosorbent assay. Statistical analysis was performed with Statistica 10 program with a critical level of significance p0.05. Results. High levels of interleukins 4, 8, 13, 17A were determined, IL7F level was not significantly different from that in control group and low level of IFNy was found in the serum of children with allergic diseases. The number of CD3CD8CD45RO, CD3CD8CD45RACD45RO Tlymphocytes, CD3CD4 Thelper cells and Th17 were increased and at the same time CD3CD4CD45RO memory cells were decreased In bronchial asthma and allergic rhinitis children. Number of Th17 cells in healthy children was 9.491.6, in allergic children it was significantly higher 14.50.77 (p0.001). Analyses of serum cytokine count in children with isolated BA and in association with allergic rhinitis revealed multidirectional correlations differing in strength and direction from those in the group of healthy children. Conclusion. In children with isolated bronchial asthma and associated with allergic rhinitis the following parameters were found: CD3CD4 Tcells count was comparable to that in healthy children, the imbalance of Thelper subpopulation: prevalence of Th2 and Th17, activation of IL17A, IL4, IL8, IL13 synthesis and low level of serum IFNy.

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Indicators phenotype lymphocytes and basophils in children with asthma

Immunopathology Allergology Infectology ◽

10.14427/jipai.2016.4.20 ◽

2016 ◽

pp. 20-24

Author(s):

E. Asiryn ◽

P. Novikov

Keyword(s):

Reference Values ◽

Atopic Asthma ◽

Control Group ◽

Group Level ◽

Absolute Level ◽

Relative Level ◽

Lymphocyte Phenotype ◽

Study Results ◽

Children With Asthma ◽

The Absolute

The aim of our research to examine the phenotype of lymphocytes and basophils in children with atopic asthma. Materials and methods. A total of 150 children aged 5 to 18 years: 130 patients with asthma, 20 children in the control group. Determines the level of the following indicators of lymphocytes and basophils: СD3+, CD4+, СD4+CD25+, CD19+CD23+, CD203с+, СD203с+CD63+, CD203с+IgE. Results. In the study of lymphocyte phenotype determined that the level of CD4+, СD4+CD25+, CD19+ lymphocytes had no statistically significant differences from the control group of children (p>0,05). The relative level of CD3+ lymphocytes was significantly different from the control group level (p<0,05), but was within the reference values. The level of CD203с+CD63+ basophils, CD203с+IgE was significantly higher in children with asthma than children in the control group (p<0,01), while the absolute level CD203c+ basophils had no significant differences. Conclusion. The findings of the study results suggest that children with asthma the level of activated basophils statistically higher, indicating their participation in the development of the disease.

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THE EFFECT OF MAGNETO-LASER THERAPY ON THE PHENOTYPE OF BASOPHILS IN CHILDREN WITH ATOPIC ASTHMA

Visuomenės sveikata ◽

10.5200/sm-hs.2018.014 ◽

2019 ◽

Vol 29 (2) ◽

pp. 35-38

Author(s):

Elena Asiryn ◽

Pavel Novikov ◽

Volha Matsiushchanka ◽

Nadiezhda Titova ◽

Lukas Vaidelys ◽

...

Keyword(s):

Laser Therapy ◽

Bronchial Asthma ◽

Inhaled Corticosteroids ◽

Atopic Asthma ◽

Relative Level ◽

Atopic Bronchial Asthma ◽

Significant Difference ◽

Group A ◽

The Absolute ◽

Group B

In recent years there has been growing evidence to suggest a major role of basophils alongside eosinophils and mast cells in allergic inflammation. The aim of this study was to analyze the dynamics of the basophil phenotypes after the use of magneto-la-ser therapy in children with atopic bronchial asthma. Materials and methods. A total of 66 children with mild persistent atopic bronchial asthma (aged 6 to 18 years old) were examined. Group A included 34 children who received magneto-laser therapy together with basic asthma treatment (low dose of inhaled corticosteroids). Group B included 32 children who received only basic asthma therapy. The level of CD203с + , CD203с + CD63 + , CD203с + IgE + basophils was determined in peripheral blood in the beginning of the study, after 2 weeks and after 3 months. Results. A statistically significant decrease in the absolute levels of CD203с+CD63+ and CD203с + IgE + basophils and in the relative level of CD203с + IgE + among all CD203с + basophils was determined in group A after magneto-laser therapy. The comparison of group A and group B indices revealed a significant difference between the relative level of CD203с + IgE + basophils after 12-15 days from the beginning of the study. This indicator was significantly lower in group A than in group B (p<0.05). The absolute level of CD203с + IgE + basophils was significantly lower in group A in comparison with group B after 82-90 days (p<0.05). Conclusions. Magneto-laser therapy can change the phenotype of basophils in children with atopic bronchial asthma, causing suppression of proallergic pa-rameters. Considering these results there is reason to believe, that it is possible to use this method as an additional immunocorrective treatment in patients with basophilic phenotype of atopic asthma.

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Initial vegetative tone in mild atopic asthma in children

Pediatrician (St Petersburg) ◽

10.17816/ped12213-18 ◽

2021 ◽

Vol 12 (2) ◽

pp. 13-18

Author(s):

Vladimir N. Buryak ◽

Tatyana I. Antonova ◽

Maria V. Dudko ◽

Inna V. Malysheva ◽

Kirill K. Shepelenko

Keyword(s):

Nervous System ◽

Autonomic Nervous System ◽

Bronchial Asthma ◽

The Body ◽

Atopic Asthma ◽

World Health ◽

Control Group ◽

Healthy Children ◽

Autonomic Nervous ◽

Atopic Bronchial Asthma

Background. In most industrialized countries, allergic diseases affect up to 20% of the population. This pathology belongs to the most common in children: according to the World Health Organization, more than 15% of the world's child population suffers from it. In recent years, there has been a significant increase in the frequency and more severe course of these diseases, in connection with which they are considered in modern society as a major medical and social problem. Thus, the prevalence of bronchial asthma, according to domestic and foreign authors, ranges from 0.2 to 8.1%. Purpose. In order to clarify the role of the autonomic nervous system in the genesis of the mild course of atopic bronchial asthma in childhood, the features of the interaction of the sympathetic and parasympathetic divisions of the autonomic nervous system in the examined children were clarified. Materials and methods. 126 children aged 10 to 14 years were examined. Atopic bronchial asthma was diagnosed in 91 children. At the same time, 61 of them were diagnosed with an intermittent course, 30 a mild persistent course of the disease. The control group consisted of 35 healthy children also aged 10 to 14 years. Complaints, anamnesis data were studied in all children, an objective and generally accepted laboratory and instrumental examination was carried out. All examined children underwent daily monitoring of the electrocardiogram, according to the results of which, based on the analysis of time and frequency indicators of heart rate variability, a variant of the initial autonomic tone was established. Results. In children with atopic bronchial asthma, both with intermittent and mild persistent course, an absolute or relative dominance of sympathetic influences was revealed against the background of varying degrees of decrease in parasympathetic activity, which was interpreted as a compensatory reaction of the body in response to chronic allergic inflammation.

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Polymorphism of the cytokine genes (H4, ИМ) and peculiarities of immune response depending on the control over atopic bronchial asthma in children

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/rja608 ◽

2013 ◽

Vol 10 (2) ◽

pp. 23-28

Author(s):

M V Smolnikova ◽

S V Smirnova ◽

O S Tyutina ◽

S V Bychkovskaya

Keyword(s):

Bronchial Asthma ◽

Cellular Immunity ◽

Control Group ◽

Healthy Children ◽

Nucleotide Polymorphisms ◽

Promoter Regions ◽

Cytokine Genes ◽

Humoral And Cellular Immunity ◽

Atopic Bronchial Asthma ◽

Cell Counts

Background. The aim of this study was to investigate the main indices of humoral and cellular immunity, the levels of cytokines (IL-2, IL-4, IL-10, TNFa) and polymorphism of promoter regions of cytokine genes (IL4 and IL10) in children with atopic bronchial asthma (ABA) with different levels of disease control. Methods. We have analyzed 110 children with ABA - controlled (CABA, n=59) and uncontrolled (UABA, n=51) and healthy children (control group, n=52). Parameters of cellular immunity were determined by fluorescence microscopy using monoclonal antibodies to surface receptors. Parameters of humoral immunity and cytokine levels in the samples of serum were measured by ELISA. Genotyping of single-nucleotide polymorphisms in the IL4 (С-590Т) and IL10 (С597А) genes was performed by PCR. Results. The level of TNFa and the relative amount of CD8+ cells was increased, while the counts of CD3+ cells and the relative amount of CD4+ cells was decreased in UABA as compared to CABA. In CABA, lower concentration of the IL-10 in serum associated with the IL10 A-597 allele was observed. The IL4 T-590 allele tends to be associated with non-controlled ABA. Conclusion. The level of TNFa, and the CD3+, CD4+ and CD8+ cell counts were identified as markers of uncontrolled course of ABA in children. Polymorphic variants of IL4 and IL10 genes can be considered as candidate markers of uncontrolled ABA.

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Neonatal Jaundice: The Other Side of the Coin in the Development of Allergy

American Journal of Perinatology ◽

10.1055/s-0039-1693697 ◽

2019 ◽

Vol 37 (13) ◽

pp. 1357-1363

Author(s):

Heba Safar ◽

Asmaa Y. Elsary

Keyword(s):

Allergic Rhinitis ◽

Bronchial Asthma ◽

Neonatal Jaundice ◽

Allergic Diseases ◽

University Hospital ◽

Control Group ◽

Healthy Children ◽

Maternal Characteristics ◽

Acute Urticaria ◽

Different Characteristics

Abstract Objective The current study aims to assess the association between different characteristics of neonatal jaundice and common types of allergic diseases in childhood (as bronchial asthma, acute urticaria, and allergic rhinitis). Study Design A case-control study is conducted on 300 allergic children and 300 healthy children as a control group at Fayoum University Hospital. The study was conducted over a span of 2 years, from May 2016 to May 2018. Bronchial asthma, allergic rhinitis, and acute urticaria diagnoses were based on physician clinical examination using specific guidelines for each. For the data regarding children's demographic and maternal characteristics, a structured questionnaire was used. Regarding neonatal jaundice, data were collected from the patients' hospital records. Results Children with neonatal jaundice are more likely to develop allergy with 57% higher than neonates without jaundice. Early onset jaundice and treatment by phototherapy have statistically significant association with the development of allergic diseases. Conclusion Different aspects of neonatal jaundice are associated with the development of common allergic diseases in children.

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The Potential Role of The Long non-coding RNA MEG3 in Bronchial Asthma in children

QJM ◽

10.1093/qjmed/hcab088.002 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

Basma Tarek Rezk ◽

Fayda Ibrahim Abdel Motaleb ◽

Terez Boshra Kamel ◽

Enas Samir Nabih ◽

Marwa Ali Abd El-Khalek

Keyword(s):

Bronchial Asthma ◽

Airflow Obstruction ◽

Response To Therapy ◽

Control Group ◽

Healthy Children ◽

Asthmatic Children ◽

Clinical Biomarkers ◽

Asthma In Children ◽

Reversible Airflow Obstruction

Abstract Aim and Background Asthma is an inflammatory disease affecting the airways of the lungs being characterized by reversible airflow obstruction and bronchospasm. Environmental and genetic factors play a role in the pathogenesis of the disease; these factors are controlled by epigenetic mechanisms. There is a demand of clinical biomarkers for diagnosis of asthma and monitoring the response to therapy. In this study, we aimed to evaluate the role of lnc-MEG3 expression and its downstream effector in the pathogenesis of bronchial asthma disease. Methods Based on bioinformatics analysis using online databases, the current work has been designed to study the expression status of lnc-MEG3 and GDF15 mRNA in serum and sputum of asthmatic children aiming to find its clinical significance. This study was conducted on 30 children, 15 children newly diagnosed with bronchial asthma as patient group (A) and 15 age and sex matched healthy children as control group (B). Quantitative PCR was carried out to evaluate the expression pattern of selected biomarkers in serum and sputum of asthmatic children. Results The expression of circulating lnc-MEG3 was highly significantly up-regulated in asthmatic children than in control group (p ≤ 0.01), while the expression of circulating GDF15 mRNA was highly significantly down-regulated in asthmatic children than in control group (p ≤ 0.01). The findings showed a negative significant correlation between both investigated biomarkers. Conclusion This study supports the role of lnc-MEG3 and GDF15 protein as potential biomarkers in diagnosis of bronchial asthma in children.

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Populational composition of CD4+5RA/ CD4+5RO positive t lymphocytes and cytokine profile in children with allergic respiratory diseases

Medical Immunology (Russia) ◽

10.15789/1563-0625-pco-2009 ◽

2021 ◽

Vol 23 (1) ◽

pp. 149-156

Author(s):

A. I. Turyanskaya ◽

N. G. Plekhova ◽

V. A. Sabynych ◽

E. V. Procekova

Keyword(s):

T Lymphocytes ◽

Bronchial Asthma ◽

Peripheral Blood ◽

Respiratory Diseases ◽

Allergic Inflammation ◽

Allergic Diseases ◽

Control Group ◽

Healthy Children ◽

Allergic Disorders ◽

T Helpers

The changing states of T cell populations responsible for the chronic course of allergic inflammation and diseases, including allergic bronchial asthma, are not yet sufficiently characterized. The aim of this study was to detect phenotypic changes in the CD45RA/CD45RO positive T lymphocytes and the level of regulatory cytokines (TNFα, IFNγ, IL-4, IL-6, IL-8, IL-10, IL-13, IL-17А, IL-17F) in allergic respiratory diseases (ARD) in children. In blood of 90 children aged 3-11 (60 children with ARD and 30 healthy peers) were studied of the immune cellular populations and cytokine indices. The levels of IL-4, IL-8, IL-10, IL-13, IL-17A and IL-17F in blood serum of children with bronchial asthma and allergic rhinitis differed from appropriate indices in control group (p = 0.001). The quantity of CD3+CD8+CD45RACD45RO+ cells, T helpers (p < 0.05) and Th effectors simultaneously expressing both isoforms of the CD45RA+ and CD45RO receptor in peripheral blood of children with ARD significantly exceeded those in control group (p < 0.001). In healthy children, Th17 population (CD3+CD4+CD196 lymphocytes) comprised 9.49±1.6% of CD3+CD4+ of cells, the number of such lymphocytes was significantly increased to 14.5±0.77 in children with allergic diseases (p < 0.001). Absolute numbers of Th17+ cells were 93.0±9.30 and 127,0±72.0 cells/µl respectively (p = 0.002). Indicators of CD4CD45RO positive memory cells in children with ARD was determined as significantly lower (p < 0.001), whereas quantity of CD3+CD19+ proved to be higher (p < 0.05) than in healthy peers. Absolute counts of these cells did not differ between the groups. The number of CD8+CD45RO+T lymphocytes was significantly higher in children with allergic diseases (p < 0.025). This research shows that the quantitative ratio of CD3+CD8+CD45RA+ and CD3+CD8+CD45RO+ populations of T cells, and increased levels of cytokines, synthesizable via Th2 and Th17, in peripheral blood may be helpful for understanding genesis of allergic respiratory diseases, and extends our knowledge on immune mechanisms of allergic disorders for individualization of therapeutic programs.

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THE EFFECT OF MAGNETO-LASER THERAPY ON THE PHENOTYPE OF BASOPHILS IN CHILDREN WITH ATOPIC ASTHMA

Visuomenės sveikata ◽

10.5200/sm-hs.2019.014 ◽

2019 ◽

Vol 29 (2) ◽

pp. 35-38

Author(s):

Elena Asiryn ◽

Pavel Novikov ◽

Volha Matsiushchanka ◽

Nadiezhda Titova ◽

Lukas Vaidelys ◽

...

Keyword(s):

Laser Therapy ◽

Bronchial Asthma ◽

Inhaled Corticosteroids ◽

Atopic Asthma ◽

Relative Level ◽

Atopic Bronchial Asthma ◽

Significant Difference ◽

Group A ◽

The Absolute ◽

Group B

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THE IMPACT OF ASTHMAAND ITS MEDICATIONS ON DENTAL CARIES STATUS AND STREPTOCOCCUS MUTANS COUNT IN CHILDREN: A CROSS-SECTIONAL STUDY

10.36106/ijsr/7515919 ◽

2020 ◽

pp. 20-23

Author(s):

Sneha Upadhyay ◽

Jyoti Bhavthankar ◽

Mandakini Mandale ◽

Nivedita Kaorey

Keyword(s):

Dental Caries ◽

Streptococcus Mutans ◽

Cross Sectional Study ◽

Control Group ◽

Healthy Children ◽

Sectional Study ◽

Cross Sectional ◽

Asthmatic Children ◽

Significant Difference ◽

The Impact

Background: Asthma and its medications have been linked to oral diseases in asthmatic children. Aim: Assessment of the dental caries status, salivary Streptococcus mutans count and S. mutans colony score in children receiving inhaled anti asthmatic medications and their comparison in healthy children Material and Method: A cross-sectional study was performed on 40 asthmatic children and 40 healthy children in the age group of 6-14 years. DMFT/deft indices were calculated and saliva samples were collected. Diluted saliva was inoculated on MSB agar plates. S. mutans count and colony score were analysed after 24-48 hours of inoculation. Results: Statistically significant difference was observed in the mean DMFT/deft index, salivary S. mutans load and S. mutans colony score in children of the asthmatic group and control group. Conclusion: Prevalence of dental caries and cariogenic bacteria is higher in asthmatic children.

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