Effects of Seed Storage Methods and Shading on Seed Germination and Seedling Growth of Endangered Species, Iris dichotoma and Iris setosa

Manglietia crassipes, a critically endangered species narrowly distributed on Mount Dayao in Guangxi, China, is also a species of ornamental interest whose variability has not been explored. Key factors leading to its endangerment have also not been studied. Here, two experiments were conducted to test the effects of different plant growth regulators and different storage conditions on germination characteristics of M. crassipes seeds. Fruit morphology was measured, and germination characteristics of fresh mature seeds were tested in order to assess natural seed vigor. Seeds were soaked in distilled water (control), or gibberellic acid (GA3), 6-benzylaminopurine (6-BA) or indoleacetic acid (IAA) solutions of different concentrations, for 48 h to determine their effects on seed germination. In addition, the effects of different seed storage conditions (constant 4 °C, −7 °C, −20 °C, or 25 °C for 100 days, wet stratification at 4 °C for 100 days) on seed germination were investigated. Results showed that the abortive rate of the mature fruits was high (28.9%) and fresh natural seeds had a low germination rate (G) and germination index (GI). Seed germination was inhibited with 6-BA at all concentrations, but only at less than 100 m·L−1 IAA; otherwise, IAA slightly promoted seed germination. GA3 broke seed dormancy and significantly accelerated seed germination by improving G, GI and initial germination time (IGT), especially over 2500 mg·L−1. The viability of seeds declined no matter how they were stored. However, 4 °C wet stratification storage was preferable for seed vigor and germination power. Our results suggest that the high abortive rate of fruits, low germination of the natural seed, seed dormancy and its intolerance to storage, contributed to the endangerment of M. crassipes. GA3 can break M. crassipes seed dormancy, which can be a benefit for future ornamental breeding and further protection or conservation.

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Effects of ultra-dry storage on seed germination and seedling growth of Handeliondendron bodinieri

Silva Fennica ◽

10.14214/sf.10509 ◽

2021 ◽

Vol 55 (3) ◽

Author(s):

Chuan Xie ◽

Tianfeng Liu ◽

Song Guo ◽

Jian Peng ◽

Zailiu Li

Keyword(s):

Polyethylene Glycol ◽

Seed Germination ◽

Moisture Content ◽

Seedling Growth ◽

Germination Rate ◽

Soluble Sugar ◽

Seed Storage ◽

Seed Vigor ◽

Protein Accumulation ◽

Dry Storage

(H. LÃ©v.) Rehder is a rare, endangered, and therefore, protected tree species native to China. However, there are serious limitations to the effective protection of the species, including a low seed germination-rate and difficult storage due to a high seed oil-content. Here, we evaluated the feasibility of ultra-dry seed storage and its effects on seedling growth. We used the silica gel method to prepare ultra-dry seeds with different moisture contents to find an optimal moisture content range (2.54%â4.77%). Ultra-dry treatment improved storability of . Furthermore, seeds with a moisture content of 4.77% stored at room temperature, and seeds with a moisture content of 3.97% stored at 4 Â°C yielded the best results. Priming with an appropriate concentration of polyethylene glycol had a certain repairing effect on ultra-dry stored seeds and improved seed vigor, with a two-day priming treatment with 20% polyethylene glycol having the best effect. Finally, compared with sand storage at 4 Â°C, ultra-dry storage promoted seedling growth and root development; furthermore, it alleviated storage damage to seeds, promoted soluble sugar and soluble protein accumulation, and increased seedling nitrogen, phosphorus, and potassium uptake. Therefore, ultra-dry storage can be effectively used to preserve seeds. Specifically, low-temperature storage of ultra-dry seeds with a moisture content of 3.97% enhanced seed vigor, and seedling growth and development.Handeliodendron bodinieriH. bodinieri seedsH. bodinieriH. bodinieriH. bodinieri

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Effect of seed storage on seed germination and seedling quality of Festulolium in comparison with related forage grasses

Tropical Grasslands - Forrajes Tropicales ◽

10.17138/tgft(8)125-132 ◽

2020 ◽

Vol 8 (2) ◽

pp. 125-132

Author(s):

Rade Stanisavljevic ◽

Dobrivoj Poštić ◽

Ratibor Štrbanović ◽

Marijenka Tabaković ◽

Snežana Jovanović ◽

...

Keyword(s):

Seed Germination ◽

Seedling Growth ◽

Lolium Multiflorum ◽

Seed Viability ◽

Seed Storage ◽

Italian Ryegrass ◽

Meadow Fescue ◽

High Germination ◽

Artificial Seed ◽

Seed Ageing

Tests of seed germination, seed dormancy and seedling growth were performed on 0-, 6-, 20- and 30-months-old seed lots of Festulolium in comparison with Italian ryegrass (Lolium multiflorum) and meadow fescue (Festuca pratensis). Tests were performed on seeds harvested in 2 different years (2014 and 2015) resulting in no major difference between the years. Seed storage affected seed viability and dormancy and seedling growth in all 3 grasses. The maximum germination of Festulolium seeds was achieved 6 months after harvest (95% normal seedlings); germination decreased significantly thereafter. While maximum germination of L. multiflorum and F. pratensis seeds was also achieved following storage for 6 months, these germination rates (93 and 90%, respectively) were retained until at least 20 months in storage. After storage for 30 months, seed germination of Festulolium, L. multiflorum and F. pratensis had declined to 72, 79 and 83%, respectively. High germination in all species was associated with higher rates of seedling growth. In an artificial seed ageing test, a temperature of 41 °C (during 48 and 72 hours) was found to effectively rank seed lots for germination performance in all 3 grasses. This test seems to have application for use in the seed trade to identify seed lots which could deteriorate more rapidly in storage. Further studies are needed to verify this hypothesis.

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IMPACT OF FERTILIZERS APPLICATION ON SEED GERMINATION AND SEEDLING GROWTH OF VIGNA RADIATA

FLORA AND FAUNA ◽

10.33451/florafauna.v24i2pp323-329 ◽

2018 ◽

Vol 24 (2) ◽

Author(s):

SUPRIYA DIXIT ◽

R. K. GUPTA

Keyword(s):

Seed Germination ◽

Leaf Area ◽

Seedling Growth ◽

Vigna Radiata ◽

Agricultural Research ◽

Dry Weight ◽

Fertilizer Application ◽

Research Field ◽

Chemical Fertilizer ◽

Seedling Length

Currently, a real challenge for the workers in the agricultural research field is to stop or reduce the use of expensive agrochemicals/ chemical fertilizers which are hazardous to the environment as well as human health. Present study was aimed to improve the growth and obtain optimum yield of Vigna crop with eco-friendly, non-toxic way and to reduce the use of agrochemical/chemical fertilizer application in agricultural activities. A pot experiment was conducted to study the effect of chemical fertilizer (DAP) and biofertilizer ( Rhizobium strain) separately and in combination on seed germination and seedling growth (at 30 days) based on morphological parameters such as seedling length (cm), fresh weight (g), dry weight (g) and leaf area (cm)2 of Vigna radiata (L.) Wilczek. After one month (30 Days) observations, it was found that seedling length, fresh and dry weights and leaf area were maximum in T4 and minimum in T15, T7 and T8 favored improved seedling length and leaf area whereas T7, T8, and T9 favored improved fresh and dry weights as compared to control.

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Allelopathic effects of Bermuda grass (Cynodon dactylon L.) root exudates on seed germination and seedling growth of Tall fescue (Festuca arundinacea Schreb)

Allelopathy Journal ◽

10.26651/allelo.j./2018-44-1-1150 ◽

2018 ◽

Vol 44 (1) ◽

pp. 25-34

Author(s):

Z.Y. Liang ◽

X.Y. Li ◽

H. Zhang ◽

J. Li ◽

X.J. Bian ◽

...

Keyword(s):

Seed Germination ◽

Seedling Growth ◽

Root Exudates ◽

Tall Fescue ◽

Festuca Arundinacea ◽

Cynodon Dactylon ◽

Bermuda Grass ◽

Allelopathic Effects

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ALLELOPATHIC EFFECTS OF VOLATILE COMPOUDS FROM CULLEN CORYLIFOLIA MEDIK. ON SEED GERMINATION AND SEEDLING GROWTH OF CUCUMIS SATIVUS L.

10.29369/ijrbat.2018.01.i.0053 ◽

2018 ◽

Author(s):

B. K. Avchar B. K. Avchar ◽

Keyword(s):

Seed Germination ◽

Cucumis Sativus ◽

Seedling Growth ◽

Cucumis Sativus L ◽

Allelopathic Effects

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Storage of primed pelleted sugar beet seed with minimal loss of seed vigour and active ingredients

Sugar Industry ◽

10.36961/si20315 ◽

2019 ◽

pp. 89-92

Author(s):

Martijn van Overveld ◽

Martijn Leijdekkers ◽

Noud van Swaaij

Keyword(s):

Sugar Beet ◽

Storage Temperature ◽

Seed Storage ◽

Cold Test ◽

Test Plant ◽

Active Ingredients ◽

Seed Vigour ◽

Plastic Bag ◽

Commercial Sugar ◽

Storage Methods

Different seed storage methods, varying in storage temperature, moisture and/or oxygen content, were applied to commercial sugar beet seed lots from four breeding companies. After storage for 10–11 months, germination of the seed was tested in the laboratory (cold test, 10°C). In addition, the contents of active ingredients (fungicides and insecticide) were analyzed and compared with the initial contents before storage. Based on these results, a selection of the most promising storage methods was made to test plant emergence in a field experiment. This research was performed in 2015/16 and in 2016/17. In both years, two storage treatments outperformed the others: these were storage in a closed jar with the addition of moisture absorber (i.e. silica gel) at room temperature and storage at –18°C in a closed plastic bag. Using these two storage methods, seed vigour and contents of active ingredients were comparable to those in seed that had not been stored for one year. Based on the results from this study, the advice to growers for a successful storage of residual sugar beet seed was adjusted in 2017, after including some practical guidelines and considerations.

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