scholarly journals Effect of seed storage on seed germination and seedling quality of Festulolium in comparison with related forage grasses

2020 ◽  
Vol 8 (2) ◽  
pp. 125-132
Author(s):  
Rade Stanisavljevic ◽  
Dobrivoj Poštić ◽  
Ratibor Štrbanović ◽  
Marijenka Tabaković ◽  
Snežana Jovanović ◽  
...  

Tests of seed germination, seed dormancy and seedling growth were performed on 0-, 6-, 20- and 30-months-old seed lots of Festulolium in comparison with Italian ryegrass (Lolium multiflorum) and meadow fescue (Festuca pratensis). Tests were performed on seeds harvested in 2 different years (2014 and 2015) resulting in no major difference between the years. Seed storage affected seed viability and dormancy and seedling growth in all 3 grasses. The maximum germination of Festulolium seeds was achieved 6 months after harvest (95% normal seedlings); germination decreased significantly thereafter. While maximum germination of L. multiflorum and F. pratensis seeds was also achieved following storage for 6 months, these germination rates (93 and 90%, respectively) were retained until at least 20 months in storage. After storage for 30 months, seed germination of Festulolium, L. multiflorum and F. pratensis had declined to 72, 79 and 83%, respectively. High germination in all species was associated with higher rates of seedling growth. In an artificial seed ageing test, a temperature of 41 °C (during 48 and 72 hours) was found to effectively rank seed lots for germination performance in all 3 grasses. This test seems to have application for use in the seed trade to identify seed lots which could deteriorate more rapidly in storage. Further studies are needed to verify this hypothesis.

Author(s):  
O.A. Zadorozhna ◽  
M.V. Herasimov ◽  
T.P. Shyianova

The seed viability of 86 acessions of fodder grasses: awnless brome (Bromopsis inermis (Leyss.) Holub.), orchardgrass (Dactylis glomerata L.), Timothy grass (Phleum pretense L.), crested wheat grass (Agropyron cristatum (L.) Gaertn.), clustered wheat grass (Agropyron desertorum (Fisch. ex Link) Schult. et Scult.f.), annual ryegrass (Lolium multiflorum Lam.), perennial ryegrass (L. perenne L.), red fescue (Festuca rubra L.), meadow fescue (Festuca pratensis Huds.), gigantea fescue (Festuca gigantea (L.) Vill.), blue fescue (Festuca inarmata Schur. (Festuca amethystina (Hack. ex Boiss.) St.-Yves)), tall fescue (Festuca arundinacea Schreb.), tall oat-grass (Arrhenatherum elatius (L.) P. Beauv. ex J. S. et K. B. Presl.), slender wheat grass (Roеgneria trachycaulon (Link) Nevski), creeping bent grass (Agrostis alba L.), redtop (Agrostis gigantea Roth.), which were stored under controlled temperature and moisture content 3 – 6 % was investigated. It has been determined that the seeds of these species have different longevity at different storage temperatures under the genebank conditions. Phleum pretense, Phleum pretense, Agropyron cristatum, Lolium multiflorum Festuca rubra seed viability was without changing after storage at temperature 4oC for 10 years and longer. The best seed longevity of grasses were achieved when the seeds are kept at –20oC. Accessions features of different grasses species seed storage are discussed.


Author(s):  
Afonso Henrique Schaeffer ◽  
Diógenes Cecchin Silveira ◽  
Otávio Augusto Schaeffer ◽  
Nadia Canali Lângaro ◽  
Leandro Vargas

2022 ◽  
Vol 354 (11-12) ◽  
pp. 97-99
Author(s):  
M. A. Starostina ◽  
N. G. Lapenko

Relevance. Biological features (morphometric indicators, viability and germinating power) of Poterium polygamum seeds found in natural communites of the Stavropol Territory have been studied. The conservation of wild-growing plant species in the natural population and the rational use of their potential are relevant.Methods. Years of study — 2013–2021. The subject of the study is Poterium polygamum seeds. Seeds were collected in 2013. Studies were carried out according to the methods of studying seed material. Freshly harvested seeds and seeds of different shelf life were germinated in petri dishes at a temperature of 18–20 °C.Results. The collected seeds of Poterium polygamum are quite large. The length of the seeds is 4–5 mm, the width is about 2.5 mm. The mass of 1000 pieces of seeds on average was 10.59 grams. Complete absence of viability of freshly harvested Poterium polygamum seeds was revealed. After three months, seed viability was 97%. This is a shallow physiological rest associated with the post-harvest maturation period. During the storage of seeds, their high germination was established — from 70 to 100%. The duration of seed storage is 8 years. The largest number of germinated seeds and their germinating power were noted in the third year of their storage (100%). Seeds germinated on the third or fourth day. The differences between the viability of seeds and their germinating power are not great. In the eighth year of storage of Poterium polygamum seeds, viability and germinating power were slightly reduced. The storage period of seeds, in which they retain the ability to germinate and the germinating power, is long. It has been found that the wild species Poterium polygamum belongs to plants with a high (70 to 100%) viability. This species is regularly resumed in the natural population. The revealed biological features of Poterium polygamum seeds can become the scientific basis during the reconstruction of degraded natural grass stand and in selection work.


2005 ◽  
Vol 15 (1) ◽  
pp. 29-42 ◽  
Author(s):  
R.C. Thapliyal ◽  
S.S. Phartyal

This paper describes the dispersal–germination characteristics of seeds of 77 native tree species in a seasonal monsoon forest in Uttaranchal state, northern India. Results indicate that 50% of the species dispersed in the hot, dry summer months, 18% during the rainy season, 23% during the cold season and the remainder in late spring. Germination tests on fresh and laboratory-stored seeds revealed a relationship between morphological features of the fruit and both germination percentage and mean germination time (MGT). Highest mean germination (50%) was for dry-dehiscent fruits with winged wind-dispersed seeds, followed by dry-dehiscent fruits with non-winged seeds (38%) and seeds of dry-indehiscent fruits (37%). Lowest germination (29%) was for seeds from fleshy or pulpy fruits. MGT followed the reverse course. Germination data for seeds stored dry in the laboratory during one seeding cycle indicated six patterns of seed germination: (1) average germination percentage of fresh seeds lower than that of stored seeds, indicating an after-ripening requirement; (2) initial high germination percentage followed by low values, indicating a steep to moderate decline in viability following harvest; (3) no germination after 1 or 2 months of seed storage, due to complete loss of viability, indicating short seed longevity; (4) fresh seed germination in some species equalled the average germination value of stored seeds, indicating constant germination for one whole seeding cycle; (5) germination of both fresh and stored seeds remained consistently low throughout the season, indicating a requirement for some kind of pre-treatment or having poor quality of seeds; (6) initial high germination followed by decline and again increase, showing a seasonal rhythm of germination.


2019 ◽  
Vol 49 ◽  
Author(s):  
Ángel Cabello ◽  
Nicolás Espinoza ◽  
Sergio Espinoza ◽  
Antonio Cabrera ◽  
Rómulo Santelices

Background: Nothofagus glauca (Phil.) Krasser (Nothofagaceae, “Hualo”) is an endemic tree of the Mediterranean zone of Chile. The natural forests in this area have been severely fragmented as a result of human causes such as replacement by agricultural crops and fast-growing tree species. From 1975, these forests have declined from 900,000 ha to 145,000 ha, so it is categorised on the IUCN Red List as ‘vulnerable’. In restoring this ecosystem, efforts should focus, in part, on the propagation of quality stock. However, information on propagation systems is still insufficient. Methods: We aimed to analyse the effect of different pre-germinative treatments and sowing times on seed germination, and seedling growth and quality. The pre-germinative treatments were: (i) cold stratification; (ii) soaking in gibberellic acid (GA3) and thiourea solution; and (iii) nursery cultivation, while the sowing times were July, August and September. Results: A high germination capacity was achieved by: soaking the seeds in GA3 solution irrespective of concentration; stratifying, irrespective of period; or soaking in 7.5 mg L-1 thiourea solution, values significantly varied from that of the control treatments. The sowing time was not relevant in terms of the percentage of germination or seedling development. Stratification at 5°C for 60 days produced the best quality indices for N. glauca seedlings but no significant differences were found in any of the morphological attributes tested as a result of the pre-germinative treatments. Conclusions: The pre-germinative treatments significantly improved the germination and seedlings growth of N. glauca. Cold stratification at 5°C for 60 days is recommended as it produced suitable seedlings for field establishment. Gibberellic acid and thiourea did not produce important effects on seedling growth. Our results suggest the presence of endogenous physiological dormancy of the N. glauca seeds. The results of this study provide important information on propagation and nursery techniques of N. glauca, which can be used in restoration programmes.


2021 ◽  
Vol 182 (1) ◽  
pp. 157-167
Author(s):  
M. A. Nikolaeva ◽  
E. Yu. Varentsova ◽  
G. F. Safina

Relevance of the study. One of the ways to maintain the genetic diversity of forest-forming species is to preserve highquality seed material ex situ. However, the relationship between the diversity of pathogenic mycobiota and the duration and methods of forest tree seed storage remains underexplored. The results of research into this problem can be used in forest seed production and forest phytopathology.Materials and methods. For our study we used seeds ofРinus sylvestris L. and Picea abies (L.) Karst. of the orthodox type, harvested in the period of 1996–2011 and stored under different temperatures: +20°С, +4°С, –18°С, and in liquid nitrogen vapor (–182°С) since 2011. Prior to their storage, seed samples were dried to a moisture content of 4.2–4.4% and hermetically packed. Seed germination was tested before and after three, five and eight years of storage, following GOST 13056.6-97 standards. The level of seed infection and the composition of pathogenic fungi were assessed.Results. After eight years of storage at +20°С, pine and spruce seed germination capacity decreased by 13–60%, depending on the year of harvesting. Seed storage at –18°С and –182°С allowed us to prevent seed infection and preserve seed viability. In most cases, the germination energy and germination capacity were negatively correlated with the level of seed infection. The diversity of pathogenic (mold) fungi on the surface of seeds was represented by ten genera; the most common were saprotrophs: Aspergillus P. Micheli, Penicillium Link, Rhizopus Ehrenb., Scopulariopsis Bainier.Conclusion. At the present stage of research, the success of seed storage at low and ultralow temperatures was demonstrated. We recommend cryopreservation for the long-term storage of improved and valuable seeds.


Author(s):  
Pudji Rahardjo

Rice husk ash as desiccation material can be used to maintain seed viability in storage through its ability to absorb humidity during its storage. High relative humidity caused seed moisture content to increase so the respiration rate of seed increases and uses faster food stock. Finally the viability of seed is lost. A research on use of rice husk ash as desiccation material of cocoa seed storage was conducted in Agronomy Laboratory and Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institute. Completely Randomized Design was used in this research with treatment of rice husk ash application on cocoa seed as follows: 0 g/100 seeds (A), 5 g/100 seeds (B), 10 g/100 seeds (C), 15 g/100 seeds (D), and 20 g/100 seeds (E). This experiment used four replications. Cocoa seeds were stored in plastic bag within carton box in ambient temperature. The storage periods were 1, 2, and 3 weeks, and parameters of observation consisted of electrical conductivity of dipped water of cocoa seeds, percentage of seed germination, percentage of seed emergence, early growth parameters at one month old including seedlings height of seedling, diameter, leaf number, root length, and dry weight. The result of the experiment showed that the use of rice husk ash at 5-10 g/100 seeds could maintain electrical conductivity of dipped water at low level, percentage of seed germination at 99-100 % and percentage of seed emergence at 79-91% after two weeks storage. The use of rice husk ash at 5-10 g/100 seeds after two weeks storage affected height of cacao seedling, but did not affected stem diameters, leaf numbers, root lengths, and dry weights


1996 ◽  
Vol 127 (1) ◽  
pp. 57-65 ◽  
Author(s):  
D. Wilman ◽  
Y. Gao

SUMMARYFour grass species, three hybrids and three mixtures were grown in field swards near Aberystwyth. All swards were amply supplied with nutrients and were cut at 5-week intervals during the year of sowing (1989) and during the following 4 years. The order of the grasses in rate of establishment was: Westerwolds ryegrass > Italian ryegrass (Lolium multiflorum) > Italian ryegrass × perennial ryegrass, Italian ryegrass × meadow fescue, perennial ryegrass (Lolium perenne) > perennial ryegrass × meadow fescue, meadow fescue (Festuca pratensis) > tall fescue (Festuca arundinacea). During the sowing year as a whole, Italian ryegrass was the highest yielding grass, followed by Westerwolds ryegrass. During the remaining period (1990–93), as a whole, the highest yields were obtained from perennial ryegrass sown alone or in a mixture with tall fescue. Tall fescue sown alone was one of the lowest yielding grasses in the year of sowing, but developed to be the highest yielding in 1992 and 1993. Westerwolds ryegrass persisted least well, although some plants did survive until 1992. Italian ryegrass persisted better than Westerwolds and Italian ryegrass × meadow fescue persisted better than Italian ryegrass. Hybrid ryegrass and perennial ryegrass × meadow fescue persisted satisfactorily but with fewer tillers/m2 than perennial ryegrass or tall fescue. The yield of tall fescue in March was as high as that of Italian ryegrass in 1990 and 1991 and higher than that of any of the other grasses in 1992 and 1993; the tiller density of tall fescue was particularly high in March. The yield of mixtures (Italian ryegrass with perennial ryegrass, Italian ryegrass with tall fescue and perennial ryegrass with tall fescue) was, on average, 2·5% more than the mean of the component species when sown alone. When grown with ryegrass, tall fescue was not prominent initially but its proportion in the sward gradually increased.


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