Estrus and pregnancy rate of Simmental-Ongole Crossbred and Ongole Grade heifer after being synchronized and inseminated

The aim of the study was to examine the synchronization of estrus and artificial insemination in heifers of Ongole grade and Simental-Ongole grade. The research used 19 Ongole Grade (OG) and 18 Simental-Ongole Crossbred (SOC) heifers. This research applied purposive sampling to determine the research population. The criteria to select the population were heifers which have in mature body. The normality of heifer’s reproductive organs were identified by performing rectal palpation, then 50 mg of medroxy progesterone acetate on vaginal sponge was used to synchronized estrus. The estrus heifer was inseminated using frozen semen. The observed parameters were the number of heifers with normal and abnormal reproductive organs, estrus sign and pregnancy. Data were analyzed descriptively. This study showed that OG and SOC with abnormal reproductive organs was 1 heifer (5.56%) and 7 heifers (36.84%), respectively. The responses of estrus synchronization in heifers with normal and abnormal reproductive organs were 94.17% and 100% in OG, and 100% and 42.86% in SOC. Meanwhile, the pregnancy rate was 70.59 and 0% in OG, and 50.00 and 0% in SOC. In conclusion, the number of SOC heifers with abnormal reproductive organs is higher than OG. Estrus synchronization using 50 mg medroxy progesterone acetate increase the amount of estrus and pregnancy in heifers with normal reproductive organs.

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The influence of purified porcine relaxin on the reproductive performance of sows following artificial insemination with fresh or frozen semen

Canadian Journal of Animal Science ◽

10.4141/cjas91-027 ◽

1991 ◽

Vol 71 (1) ◽

pp. 237-239 ◽

Cited By ~ 1

Author(s):

P. A. Thacker ◽

A. D. Gooneratne ◽

R. N. Kirkwood

Keyword(s):

Key Words ◽

Pregnancy Rate ◽

Litter Size ◽

Reproductive Performance ◽

Artificial Insemination ◽

Pregnancy Rates ◽

Frozen Semen

Pregnancy rates and litter sizes following insemination of sows with fresh-extended and frozen-thawed semen averaged 71 vs. 53% and 10.6 vs. 4.4, respectively, and was not affected by the addition of 12.5 μg relaxin to semen. These results indicate no relaxin mediated effect on either pregnancy rate or litter size when sows are artificially inseminated with relaxin-supplemented semen. Key words: Artificial insemination, relaxin, sows

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Study on the effect of insemination time on pregnancy rate of Bangladeshi buffalo in intensive farming

SAARC Journal of Agriculture ◽

10.3329/sja.v16i2.40266 ◽

2019 ◽

Vol 16 (2) ◽

pp. 143-152

Author(s):

MA Hamid

Keyword(s):

Pregnancy Rate ◽

Artificial Insemination ◽

Conception Rate ◽

Transrectal Ultrasonography ◽

Livestock Breeding ◽

Intensive Farming ◽

Rectal Palpation

The study was designed to evaluate the insemination time and pregnancy rate of Bangladeshi buffalo at Lal Teer Livestock Breeding and Research Farm, Bhaluka, Mymensingh, Bangladesh during January 2017 to June 2018. A total of 30 cyclic buffaloes were included in this study. The animals were inseminated under four different times, i.e. inseminated i) between 0-6 hours after seeing first sign of estrous, ii) between 6-12 hours after seeing first sign of estrous, iii) between 12-18 hours after seeing first sign of estrous and v) between 18-24 hours after seeing first sign of estrous. The pregnancies (positive or negative) were confirmed by rectal palpation and transrectal ultrasonography after 60 days of artificial insemination (AI). The total pregnant animals in this study were 40%. The highest pregnancy rate (60%) was observed in insemination between 12-18 hours after seeing first sign of estrous and the lowest pregnancy rate (0%) was observed in insemination between 0-6 hours after seeing first sign of estrous. The findings of the study suggest that, the conception rate of buffalo depend on the time of AI, detection of proper estrus symptoms and site of semen deposition. SAARC J. Agri., 16(2): 143-152 (2018)

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19 FACTORS AFFECTING PREGNANCY RATE AFTER ARTIFICIAL INSEMINATION WITH EQUINE SEMEN: PRODUCTION OF THE FIRST FOAL USING FROZEN SEMEN IN KOREA

Reproduction Fertility and Development ◽

10.1071/rdv23n1ab19 ◽

2011 ◽

Vol 23 (1) ◽

pp. 116

Author(s):

Y. S. Park ◽

Y. G. Cho ◽

K. H. Cho ◽

G. J. Cho

Keyword(s):

Pregnancy Rate ◽

Artificial Insemination ◽

Technology Development ◽

Skim Milk ◽

Development Program ◽

Field Trials ◽

Pregnancy Rates ◽

Chi Square ◽

Semen Collection ◽

Frozen Semen

Research in the area of equine artificial insemination (AI) has led to its increased application in field trials (Loomis and Graham, 2008). However, procedures for equine semen collection, cooling and freezing of semen and artificial insemination need further improvement. This study examined the effect of semen preparation and ovulation on equine AI success. Stallion semen was collected via artificial vagina, diluted with skim-milk extender, and preserved by either cooling to 4°C or cryopreservation and storage in liquid nitrogen. Mares were examined by ultrasound for follicle development with every 12 h during oestrus. During AI, semen was inserted into uterine horn adjacent to the ovulating follicle sized over 4 cm and irregular shape. In experiment 1, mares were inseminated with either cooled, cooled-transported, or frozen–thawed semen. Cooled semen was used immediately after collection and cooling. Cooled-transported semen was used 4 h after semen collection and dilution. Semen freezing procedure was followed by Moore et al. (2006). In experiment 2, we examined the effect of buffer (skim-milk extender), which was infused into the uterus at the time of AI with frozen–thawed semen. In experiment 3, we compared AI pregnancy rates for mares ovulating spontaneously v. after treatment with hCG. Pregnancy data were analysed Chi square test where P-values < 0.05 were considered significantly different. In experiment 1, the pregnancy rates after AI using cooled, cooled-transported, and frozen–thawed semen were 60 (10/6), 50 (8/4), and 37.5% (8/3), respectively, and similar among treatments. In experiment 2, the pregnancy rate of mares infused with buffer at AI was 37.5% (8/3) which was higher than that with no buffer (0%; 6/0). In experiment 3, the pregnancy rates of mares were similar between ovulated spontaneously (25%; 8/2) and ovulated with hCG (50%; 8/4). The results suggest that equine semen that has been cooled, cooled and transported or frozen can be successfully used to establish AI pregnancy. Also, the pregnancy rates after AI can be increased by infusing buffer into the uterus at AI or by inducing ovulation with hCG. These experiments resulted in the first foal produced in Korea with frozen–thawed equine semen. This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

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Inbreeding Depression and Alternative Solution in Buffaloes

Indonesian Bulletin of Animal and Veterinary Sciences ◽

10.14334/wartazoa.v28i1.1744 ◽

2018 ◽

Vol 28 (1) ◽

pp. 1

Author(s):

Lisa Praharani ◽

Riasari Gail Sianturi ◽

Chalid Talib

Keyword(s):

Growth Performance ◽

Pregnancy Rate ◽

Inbreeding Depression ◽

Artificial Insemination ◽

Fixed Time ◽

Farming System ◽

Alternative Solution ◽

Selling Price ◽

Estrus Synchronization ◽

Natural Breeding

Buff and dairy buffaloes have an important role in farming system to produce meat, milk, and fertilizer. Their productivity and population have been decreasing due to several reasons such as inbreeding depression and lack of sires. This paper presents alternative efforts to improve genetics of buffaloes through outbreeding fascilitated by artificial insemination (AI) and estrus synchronization to decrease inbreeding rates. Effort to reduce inbreeding depression is conducted by introducing new buffalo genes from distant populations known as outbreeding. Indonesian Research Institute for Animal Production has initiated an outbreeding program in buffalo through AI. The pregnancy rate varies between 40-80% using estrus synchronization with prostaglandin and fixed time AI. Outbred offsprings have higher growth performance and selling price than offsprings of natural breeding. The outbreeding program through AI in buffaloes need to be carried out sustainability to increase availability of qualified breeding stocks.

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ANGKA KEBUNTINGAN SAPI LOKAL SETELAH DIINDUKSI DENGAN PROTOKOL OVSYNCH (Conception Rates of Local Cows after Induction with Ovsynch Protocols)

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v9i2.3804 ◽

2015 ◽

Vol 9 (2) ◽

Author(s):

Mefrianti Efendi ◽

Tongku Nizwan Siregar ◽

Hamdan Hamdan ◽

Dasrul Dasrul ◽

Cut Nila Thasmi ◽

...

Keyword(s):

Pregnancy Rate ◽

Artificial Insemination ◽

Estrus Synchronization ◽

Multiple Injection ◽

Treatment Groups ◽

Group I ◽

Conception Rates ◽

Group Ii

This study aims to know the effect of estrus synchronization using ovsynch method on local cow pregnancy rate. This study used 10 local cows without pregnant status and clinically healthy. All cows were divided into 2 treatment groups. Cows in group I (K1) were synchronized estrus using 5 mg/ml PgF2α intramuscularly with multiple injection patterns with intervals of 10 days. Group II (KII) was synchronized by ovsynch protocol, initiated with injection of 1ml GnRH on day-0. On the 7th day, the whole cows in K II were injected with 2 ml PgF2α then followed by re-injected with 1 ml of GnRH on the 9th day. Artificial insemination was performed 24 hours after GnRH last injection. The data were analyzed descriptively. The results showed that 3 cows in group I were diagnosed pregnant (60%), whereas in group II, 4 cows werediagnosed pregnant (80%). In conclusion, the estrus synchronization with ovsynch protocols provide a higher pregnancy rate than the conventional estrus synchronization.Key words: synchronization, ovsynch, GnRH, PgF2α

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Factors Affecting Pregnancy Rate in Artificial Insemination with Frozen Semen During Non-Breeding Season in Murciano-Granadina Goats: a Field Assay

Reproduction in Domestic Animals ◽

10.1111/j.1439-0531.2005.00624.x ◽

2005 ◽

Vol 40 (6) ◽

pp. 526-529 ◽

Cited By ~ 22

Author(s):

I Salvador ◽

MP Viudes-de-Castro ◽

J Bernacer ◽

EA Gomez ◽

MA Silvestre

Keyword(s):

Pregnancy Rate ◽

Breeding Season ◽

Artificial Insemination ◽

Factors Affecting ◽

Frozen Semen ◽

Field Assay

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Artificial insemination by donor: a review of 12 years' experience

Journal of Biosocial Science ◽

10.1017/s0021932000014115 ◽

1982 ◽

Vol 14 (3) ◽

pp. 253-262 ◽

Cited By ~ 4

Author(s):

G. L. Foss

Keyword(s):

Pregnancy Rate ◽

Artificial Insemination ◽

Live Births ◽

Future Developments ◽

Frozen Semen

SummaryTwelve years' experience of AID in a non-NHS clinic is reviewed. Of 381 women treated, 230 became pregnant at least once; 308 pregnancies were achieved in 450 courses of treatment (68%) resulting in 263 live births (85%). For women aged over 35 years the pregnancy rate was 47%. Timing of insemination and treatment with clomiphene are described. The use of fresh or frozen semen and future developments are discussed.

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PSII-21 Vaginitis incidence and effects on fertility of beef females enrolled on estrus synchronization protocols using a controlled internal drug release (CIDR) device

Journal of Animal Science ◽

10.1093/jas/skz258.491 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 242-242 ◽

Cited By ~ 1

Author(s):

Nicholas W Dias ◽

Alvaro Sales ◽

Claire Timlin ◽

Stefania Pancini ◽

John Currin ◽

...

Keyword(s):

Drug Release ◽

Pregnancy Rate ◽

Artificial Insemination ◽

Pregnancy Rates ◽

Estrus Synchronization ◽

Pregnancy Status ◽

Timed Artificial Insemination ◽

System 1 ◽

Synchronization Protocol

Abstract Our objectives were to characterize the incidence of vaginitis caused by a controlled internal drug release (CIDR) device and its effects on fertility of beef females enrolled on estrus synchronization followed by timed-artificial insemination (TAI). A total of 1,097 mature cows and 215 heifers from 9 different locations were enrolled in the 7-d CO-Synch + CIDR synchronization protocol. At CIDR insertion, all females were inspected for any previous signs of vaginitis prior to enrollment in the experiment. As evidence of vaginitis, a CIDR score was determined at CIDR removal, and an AI plastic sheath score was also performed at TAI following a 1 to 4 system: 1=clean presenting no secretion; 2=clean presenting clear secretion; 3=presence of purulent secretion; 4=presence of purulent secretion and blood. Pregnancy status was determined by rectal ultrasonography approximately 40 days after TAI. The scores for CIDR and AI plastic sheath did not differ between cows and heifers. Of all females, 6% had a CIDR score of 1, 31% score 2, 56% score 3, and 7% score 4. Plastic sheath score was 1 for 16%, 2 for 62%, 3 for 15%, and 4 for 7% of females. Pregnancy rate to TAI differed between cows and heifers (P < 0.01), among locations (P < 0.001) and ranged from 36 to 66%; however, it was not influenced by CIDR score (P = 0.259) and plastic sheath score (P = 0.785). Pregnancy rate of females by different CIDR scores was 60%, 57%, 52%, and 46% for scores 1, 2, 3, and 4, respectively. Pregnancy rate of females by different AI plastic sheath score was 60%, 55%, 45%, and 48%, for scores 1, 2, 3, and 4, respectively. The presence of vaginitis caused by a CIDR does not influence pregnancy rates of TAI in beef females.

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PSXIII-22 Modeling the optimal time point at which to perform timed artificial insemination

Journal of Animal Science ◽

10.1093/jas/skz258.739 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 371-371

Author(s):

Jordan M Thomas ◽

William R Lamberson ◽

John B Hall

Keyword(s):

Mathematical Model ◽

Pregnancy Rate ◽

Artificial Insemination ◽

Pregnancy Rates ◽

Optimal Time ◽

Estrus Synchronization ◽

Time Points ◽

Optimal Time Point ◽

Split Time ◽

Time Point

Abstract Timing of artificial insemination (AI) critically impacts likelihood of pregnancy success. In modern timed AI protocols for cattle, AI is performed at a predetermined time point following estrus synchronization. As a result, there may be significant variation among cows or heifers in estrous status and in timing of insemination relative to estrus onset. Recommendations as to when timed AI should be performed are formulated based on research trials evaluating alternative time points. However, available data are limited, as such trials are costly and require large numbers to detect significant differences. As an alternative approach, a mathematical model was developed to predict the anticipated aggregate pregnancy rate to timed AI based on the hour at which timed AI is performed. Previously published distributions of estrus onset were compiled separately for two long-term progestin-based protocols for beef heifers (MGA-PG and 14-d CIDR-PG). Probability of pregnancy was modeled using a regression equation for fertility based on timing of AI in relation to timing of estrus, with heifers grouped in one hour time blocks. Based on a previously published meta-analysis that compiled pregnancy rates when timed AI was performed prior to onset of estrus, a reduced pregnancy rate was assigned to heifers for which timed AI would be performed prior to anticipated estrus expression. Modeled pregnancy rates based on timing of AI were plotted for each protocol. Additionally, use of a split-time AI versus fixed-time AI approach was modeled, as well as predicted impact of varying intervals between the two time points used for split-time AI. Modeled cumulative estrous response and aggregate pregnancy rates were compared to published data for the MGA-PG and 14-d CIDR-PG protocols. Results suggest a mathematical model may be useful to determine the optimal time point at which to perform timed AI following estrus synchronization.

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