Insufficient decontamination in Sewage treatment plants induce the risk of artificial selection of extended-spectrum β-lactamase producing Escherichia coli

ABSTRACTTo determine the distribution and relationship of antimicrobial resistance determinants among extended-spectrum-cephalosporin (ESC)-resistant or carbapenem-resistantEscherichia coliisolates from the aquatic environment in India, water samples were collected from rivers or sewage treatment plants in five Indian states. A total of 446E. coliisolates were randomly obtained. Resistance to ESC and/or carbapenem was observed in 169 (37.9%)E. coliisolates, which were further analyzed. These isolates showed resistance to numerous antimicrobials; more than half of the isolates exhibited resistance to eight or more antimicrobials. TheblaNDMgene was detected in 14/21 carbapenem-resistantE. coliisolates:blaNDM-1in 2 isolates,blaNDM-5in 7 isolates, andblaNDM-7in 5 isolates. TheblaCTX-Mgene was detected in 112 isolates (66.3%):blaCTX-M-15in 108 isolates andblaCTX-M-55in 4 isolates. We extracted 49 plasmids from selected isolates, and their whole-genome sequences were determined. Fifty resistance genes were detected, and 11 different combinations of replicon types were observed among the 49 plasmids. The network analysis results suggested that the plasmids sharing replicon types tended to form a community, which is based on the predicted gene similarity among the plasmids. Four communities each containing from 4 to 17 plasmids were observed. Three of the four communities contained plasmids detected in different Indian states, suggesting that the interstate dissemination of ancestor plasmids has already occurred. Comparison of the DNA sequences of theblaNDM-positive plasmids detected in this study with known sequences of related plasmids suggested that various mutation events facilitated the evolution of the plasmids and that plasmids with similar genetic backgrounds have widely disseminated in India.

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Extended-spectrum β-lactamase producing Escherichia coli in hospital wastewaters and sewage treatment plants in Queensland, Australia

Canadian Journal of Microbiology ◽

10.1139/cjm-2013-0515 ◽

2013 ◽

Vol 59 (11) ◽

pp. 737-745 ◽

Cited By ~ 19

Author(s):

Aycan Gündoğdu ◽

Amy V. Jennison ◽

Helen V. Smith ◽

Helen Stratton ◽

Mohammad Katouli

Keyword(s):

Escherichia Coli ◽

Antimicrobial Agents ◽

Sewage Treatment ◽

Point Of View ◽

Hospital Wastewater ◽

Sewage Treatment Plants ◽

E Coli ◽

Extended Spectrum ◽

Inti Gene ◽

Hospital Wastewaters

We investigated the prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in untreated hospital wastewaters and 2 sewage treatment plants (STPs). A collection of 252 ESBL-producing E. coli isolates from hospital wastewater and STPs were typed and tested for resistance to 17 antimicrobial agents and for the presence of integron-associated integrases (intI gene) and ESBL genes. Eighty-nine percent (n = 176) of the ESBL-producing E. coli strains from hospital wastewater were found in more than 1 sample (common types), with 1 common type accounting for 35% of isolates, found in all samples. These strains were also resistant to up to 9 non-β-lactam antibiotics and showed the same pattern of resistance in all samples. More than 73% of the hospital wastewater isolates possessed SHV-type ESBL as opposed to isolates from STPs that carried only CTX-M-type ESBL genes. The prevalence of the intI gene did not differ between the sources of the isolates. Certain ESBL-producing E. coli were dominant in hospital wastewaters. These strains possessed β-lactamase genes that were different from isolates found in STPs. From a public health point of view, the presence of such a high level of ESBL-producing E. coli strains in hospital wastewaters is of great importance.

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Application of Multi-Criteria Analysis in the Selection of Individual Sewage Treatment Plants

Environmental Sciences Proceedings ◽

10.3390/environsciproc2021009027 ◽

2021 ◽

Vol 9 (1) ◽

pp. 27

Author(s):

Natalia Taraszkiewicz

Keyword(s):

Sewage Treatment ◽

Sewage Treatment Plant ◽

Treatment Plant ◽

Good Alternative ◽

Living Standards ◽

Biological Wastewater Treatment ◽

Daily Lives ◽

Sewage Treatment Plants ◽

Sewage Systems ◽

Selection Of

The development of sewage systems leads to an increase in people’s living standards and an improvement in the comfort of their daily lives. In 2021, the use of septic tanks is still a big issue; many of them are not properly sealed and can be harmful to the environment because of leakage. A good alternative for them is an individual sewage treatment plant. There are many types of such investment. This paper focuses on the selection between three types of sewage treatment plants (a biological wastewater treatment plant with activated sludge and a constructed wasteland) using MCDA–AHP and TOPSIS methods.

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Survival of Escherichia coli in two sewage treatment plants using UV irradiation and chlorination for disinfection

Water Research ◽

10.1016/j.watres.2013.09.008 ◽

2013 ◽

Vol 47 (17) ◽

pp. 6670-6679 ◽

Cited By ~ 40

Author(s):

E.M. Anastasi ◽

T.D. Wohlsen ◽

H.M. Stratton ◽

M. Katouli

Keyword(s):

Escherichia Coli ◽

Uv Irradiation ◽

Sewage Treatment ◽

Sewage Treatment Plants

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Pathogenic Escherichia coli Found in Sewage Treatment Plants and Environmental Waters

Applied and Environmental Microbiology ◽

10.1128/aem.00657-12 ◽

2012 ◽

Vol 78 (16) ◽

pp. 5536-5541 ◽

Cited By ~ 49

Author(s):

E. M. Anastasi ◽

B. Matthews ◽

H. M. Stratton ◽

M. Katouli

Keyword(s):

Escherichia Coli ◽

Animal Species ◽

Sewage Treatment ◽

Environmental Waters ◽

Phylogenetic Groups ◽

Content Type ◽

Sewage Treatment Plants ◽

E Coli ◽

Nonpoint Sources ◽

Pathogenic Escherichia Coli

ABSTRACTWe previously demonstrated that someEscherichia colistrains with uropathogenic properties survived treatment stages of sewage treatment plants (STPs), suggesting that they may be released into the environment. We investigated the presence of such strains in the surrounding environmental waters of four STPs from which these persistent strains were isolated. In all, 264E. coliisolates were collected from 129 receiving water sites in a 20-km radius surrounding STPs. We also included 93E. colistrains collected from 18 animal species for comparison. Isolates were typed using a high-resolution biochemical fingerprinting method (the PhPlate system), and grouped into common (C) types. One hundred forty-seven (56%) environmental isolates were identical to strains found in STPs' final effluents. Of these, 140 (95%) carried virulence genes (VGs) associated with intestinal pathogenicE. coli(IPEC) or uropathogenicE. coli(UPEC) and were found in a variety of sites within areas sampled. Of the remaining 117 environmental strains not identical to STP strains, 105 belonged to 18 C types and 102 of them carried VGs found among IPEC or UPEC strains. These strains belonged mainly to phylogenetic groups A (A0 and A1) and B1 and to a lesser extent B22, B23, D1, and D2. Eight of 18 environmental C types, comprising 50 isolates, were also identical to bird strains. The presence of a high percentage of environmentalE. coliin waters near STPs carrying VGs associated with IPEC and UPEC suggests that they may have derived from STP effluents and other nonpoint sources.

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Occurrence of antimicrobial-resistant Escherichia coli in sewage treatment plants of South India

Journal of Water Sanitation and Hygiene for Development ◽

10.2166/washdev.2020.051 ◽

2020 ◽

Vol 10 (1) ◽

pp. 48-55 ◽

Cited By ~ 3

Author(s):

Yerabham Praveenkumarreddy ◽

Masato Akiba ◽

Keerthi Siri Guruge ◽

Keshava Balakrishna ◽

Kalwaje Eshwara Vandana ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

South India ◽

Sewage Treatment ◽

Domestic Wastewater ◽

Global Network ◽

Resistance Pattern ◽

Sewage Treatment Plants ◽

E Coli ◽

Significant Difference

Abstract Antibiotics received by sewage treatment plants may be the causative factor in spreading antibiotic resistance bacteria in the aquatic environment. The current study investigates the distribution of antimicrobial-resistant Escherichia coli (E. coli) in four sewage treatment plants (STPs) in South India receiving hospital and domestic wastewater in different proportions. A total of 221 E. coli isolates were checked for antimicrobial resistance against 16 antimicrobials. Among the antimicrobials tested, ampicillin (AMP) and cefazolin (CFZ) showed resistance between 20% and 90%, nalidixic acid (NAL) and ciprofloxacin (CIP) showed resistance between 15% and 75% and chloramphenicol (CHL) showed resistance between 2% and 20%. Based on the observations, there is no significant difference between the wastewater inlet and outlet, suggesting that treatment process was not effective in reducing the resistance. In conclusion, the trends of antimicrobial resistance pattern show that the levels of resistance were slightly higher in hospital wastewater than domestic wastewater. This article has been made Open Access thanks to the generous support of a global network of libraries as part of the Knowledge Unlatched Select initiative.

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Sanitary sewage

Periódico Eletrônico Fórum Ambiental da Alta Paulista ◽

10.17271/1980082717520213069 ◽

2021 ◽

Vol 17 (5) ◽

Author(s):

Isadora Vitali Lobo ◽

Juliana Heloisa Pinê Américo-Pinheiro

Keyword(s):

Emerging Contaminants ◽

Sewage Treatment ◽

Domestic Sewage ◽

Municipal Sewage ◽

Sewage Treatment Plants ◽

Physical Chemical ◽

Treatment And Disposal ◽

Domestic Effluent ◽

Research Period ◽

Selection Of

The treatment and disposal of domestic sewage is one of Brazil's main challenges. Sewage composition varies with the habits of the population and the frequency with which new contaminants are released into the environment. This study is a bibliographical review of the main aspects related to the characterization and composition of sanitary sewage, types of sewage treatment systems and pertinent legislation; and toxicity of domestic effluents. The review was based on publications available on Science Direct, Google Scholar and Scielo, as well as on printed publications, relevant legislation, and normative instructions. The research period adopted for the selection of publications was from 2005 to 2021. We found out that, in addition to the levels of organic matter from domestic sewage, residues from products used in daily life, such as pharmaceuticals and cleaning products, can be found in effluents in concentrations harmful to the environment. Often, the types of treatment used in municipal sewage treatment plants do not efficiently remove these contaminants. Thus, even if sewage is treated to meet the limits required for the physical-chemical and biological parameters established by law, domestic effluent can present a high potential for toxicity to various aquatic species such as microcrustaceans, mollusks and fish. Thus, ecotoxicological analyses represent a remarkable mechanism for indicating the efficiency of removal of emerging contaminants present in treated sanitary effluent, in addition to indicating the deleterious effects caused by these residues to the environment and ecosystems associated with the receiving water body.

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