Evaluation of hemodialysis patients and hemodialysis health workers with COVID-19 IgM and IgG antibody test; a multicenter study from Eskisehir, Turkey

Abstract Background Vaccination programs are essential for the containment of the COVID-19 pandemic, which has affected hemodialysis populations especially hard. Early reports suggest a reduced immunologic response to SARS-Cov-2 vaccines in dialysis patients, in spite of a high degree of seroconversion. We aimed to identify risk factors for a reduced efficacy of an mRNA vaccine in a cohort of hemodialysis patients. Method In a multicenter study, including 294 Portuguese hemodialysis patients who had received 2 doses of BNT162b2 with a three week interval, IgG-class antibodies against the SARS-CoV-2 spike protein were determined 3 weeks after the first dose (M1) and 6 weeks after the second dose (M2). The threshold for seroconversion was 10UR/mL. Demographic and clinical data was retrieved from a quality registry. Adverse events were registered using a questionnaire. Results At M2, seroconversion was 93.1% with a median antibody level of 197.5U/mL (1.2–3237.0) and a median increase of 180.0U/mL (-82.9–2244.6) from M1. Age (beta -8.9; 95%CI: -12.88 to -4.91; P < 0.0001), ferritin > 600ng/mL (beta 183.93; 95%CI: 74.75 to 293.10; P = 0.001) and physical activity (beta 265.79; 95%CI: 30.7 to 500.88; P = 0.03) were independent predictors of SARS-Cov-2 antibody levels after two vaccine doses. Plasma albumin > 3.5g/dL independently predicted the increase of antibody levels between both doses (OR 14.72; 95%CI: 1.38 to 157.45; P = 0.03). Only mild adverse reactions were observed in 10.9% of patients. Conclusions The SARS-Cov-2 vaccine BNT162b2 is safe and effective in hemodialysis patients. Besides age, iron status and nutrition are possible modifiable modulators of the immunologic response to SARS-Cov-2 mRNA vaccines. This data suggests the need for an early identification of populations at higher risk for diminished antibody production and the potential advantage of the implementation of oriented strategies to maximize the immune response to vaccination in these patients.

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Risk factors for the mortality of hemodialysis patients with COVID‐19: A multicenter study from the overall hemodialysis population in Wuhan

Seminars in Dialysis ◽

10.1111/sdi.12995 ◽

2021 ◽

Author(s):

Hui Tang ◽

Can Tu ◽

Fei Xiong ◽

Xin Sun ◽

Jian‐Bo Tian ◽

...

Keyword(s):

Risk Factors ◽

Multicenter Study ◽

Hemodialysis Patients

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Evaluation of diagnostic accuracy of developed rapid SARS-COV-2 IgG antibody test kit using novel diluent system

VirusDisease ◽

10.1007/s13337-021-00669-4 ◽

2021 ◽

Author(s):

Vani Maya ◽

Jyothi Embekkat Kaviyil ◽

Dinoop Koral Ponnambath ◽

Renjith P. Nair ◽

Anugya Bhatt ◽

...

Keyword(s):

Diagnostic Accuracy ◽

Antibody Test ◽

Igg Antibody ◽

Test Kit

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Validation of a new automated chemiluminescent anti-SARS-CoV-2 IgM and IgG antibody assay system detecting both N and S proteins in Japan

10.1101/2020.07.16.20155796 ◽

2020 ◽

Author(s):

RIn Yokoyama ◽

Makoto Kurano ◽

Yoshifumi Morita ◽

Takuya Shimura ◽

Yuki Nakano ◽

...

Keyword(s):

Measurement System ◽

False Positive ◽

Laboratory Testing ◽

Antibody Test ◽

Measurement Range ◽

Antibody Assay ◽

Serum Samples ◽

Igg Antibody ◽

Antibody Titers ◽

Pcr Test

PCR methods are presently the standard for the diagnosis of Coronavirus disease 2019 (COVID-19), but additional methodologies are needed to complement PCR methods, which have some limitations. Here, we validated and investigated the usefulness of measuring serum antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the iFlash3000 CLIA analyzer. We measured IgM and IgG titers against SARS-CoV-2 in sera collected from 26 PCR-positive COVID-19 patients, 53 COVID-19-suspected but PCR-negative patients, and 20 and 100 randomly selected non-COVID-19 patients who visited our hospital in 2020 and 2017, respectively. The within-day and between-day precisions were regarded as good, since the coefficient variations were below 5%. Linearity was also considered good between 0.6 AU/mL and 112.7 AU/mL for SARS-CoV-2 IgM and between 3.2 AU/mL and 55.3 AU/mL for SARS-CoV-2 IgG, while the linearity curves plateaued above the upper measurement range. We also confirmed that the seroconversion and no-antibody titers were over the cutoff values in all 100 serum samples collected in 2017. These results indicate that this measurement system successfully detects SARS-CoV-2 IgM/IgG. We observed four false-positive cases in the IgM assay and no false-positive cases in the IgG assay when 111 serum samples known to contain autoantibodies were evaluated. The concordance rates of the antibody test with the PCR test were 98.1% for SARS-CoV-2 IgM and 100% for IgG among PCR-negative cases and 30.8% for SARS-CoV-2 IgM and 73.1% for SARS-CoV-2 IgG among PCR-positive cases. In conclusion, the performance of this measurement system is sufficient for use in laboratory testing.

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SARS-CoV-2 Immunoglobulin g (IgG) Kinetics in Healthcare Workers and Their Close Contacts Reduced Risk of Re-infection: South-East Asian Region

10.21203/rs.3.rs-540165/v1 ◽

2021 ◽

Author(s):

Mradul Kumar Daga ◽

Govind Mawari ◽

Vijay Kumar Karra ◽

Meghachandra Singh ◽

Siddharth Chand ◽

...

Keyword(s):

Immunoglobulin G ◽

Healthcare Workers ◽

Close Contact ◽

Antibody Test ◽

New Delhi ◽

Igg Antibody ◽

Symptomatic Infection ◽

Medical College ◽

Reduced Risk ◽

Close Contacts

Abstract BackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for global pandemic, and it has caused more than 2.3 million deaths. Persistence and stability of immunoglobulin G (IgG) response after recovery from COVID-19 infection is still uncertain. MethodsWe performed a longitudinal cohort study in healthcare workers (HCW) and their close contacts (Non-HCW) with known resolved SARS-CoV-2 infection and undiagnosed infection at Maulana Azad Medical College and associated Lok Nayak hospital, New Delhi. Baseline IgG antibody titers was determined and the participants were followed over a period of six months. We also examined relationship between SARS-CoV-2 immunoglobulin G (IgG) response and new symptomatic infection in HCW and Non-HCW over time. Results176 (70.9%) healthcare workers and 72 (29.0%) non-healthcare workers were recruited from two cohorts. 82 subjects recovered from SARS-CoV-2 infection and 166 undiagnosed for the infection having history of close contact with COVID-19 patients were followed up for a median of 227 days (interquartile range, 166 to 202) after a positive IgG antibody test. In the recovered subjects 70.7% (58) were seropositive for first anti-spike IgG assay at baseline, followed by 80.0%, 90.6% and 82.6% at three visits respectively. In undiagnosed subjects 37.3% (62) were seropositive at baseline, followed by 70.9%, 75.8% and 82.2% respectively. Also, 46.8% (29) were asymptomatic with no symptoms of COVID-19 and were seropositive at baseline. However, presence of IgG antibodies was associated with substantial reduced risk of re-infection over the follow up duration.ConclusionOur data showed that the antibodies levels measured increased over the first three months and decreased slightly after that and remained at a plateau and relatively stable for at least a period of six months.

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Performance verification of anti-SARS-CoV-2-specific antibody detection by using four chemiluminescence immunoassay systems

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/0004563220963847 ◽

2020 ◽

Vol 57 (6) ◽

pp. 429-434

Author(s):

Yafang Wan ◽

Zhijie Li ◽

Kun Wang ◽

Tian Li ◽

Pu Liao

Keyword(s):

False Positive ◽

Detection System ◽

False Positive Rate ◽

Antibody Test ◽

The Other ◽

Antibody Detection ◽

Igg Antibody ◽

Chi Square ◽

Chemiluminescence Immunoassay ◽

Chi Square Test

Objectives The purpose of the current study was to evaluate the analytical performance of seven kits for detecting IgM/IgG antibodies against coronavirus (SARS-CoV-2) by using four chemiluminescence immunoassay systems. Methods Fifty patients diagnosed with SARS-CoV-2 infection and 130 controls without coronavirus infection from the General Hospital of Chongqing were enrolled in the current retrospective study. Four chemiluminescence immunoassay systems, including seven IgM/IgG antibody detection kits for SARS-CoV-2 (A_IgM, A_IgG, B_IgM, B_IgG, C_IgM, C_IgG and D_Ab), were employed to detect antibody concentrations. The chi-square test, the receiver operating characteristic (ROC) curve and Youden’s index were determined to verify the cut-off value of each detection system. Results The repeatability verification results of the A, B, C and D systems are all qualified. D_Ab performed best (92% sensitivity and 99.23% specificity), and B_IgM performed worse than the other systems. Except for the A_IgM and C_IgG systems, the optimal diagnostic thresholds and cut-off values of the other kits and their recommendations are inconsistent with each other. B_IgM had the worst AUC, and C_IgG had the best diagnostic accuracy. More importantly, the B_IgG system had the highest false-positive rate for testing patients with AIDS, tumours and pregnancies. The A_IgM system test showed the highest false-positive rates among elderly individuals over 90 years old. COVID-2019 IgM/IgG antibody test systems exhibit performance differences. Conclusions The Innodx Biotech Total Antibody serum diagnosis kit is the most reliable detection system for anti-SARS-CoV-2 antibodies, which can be used together with nucleic acid tests as an alternative method for SARS-CoV-2 detecting.

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SP400UTILITY OF NEW ANTHROPOMETRIC INDEXES IN HEMODIALYSIS PATIENTS: CROSS-SECTIONAL MULTICENTER STUDY

Nephrology Dialysis Transplantation ◽

10.1093/ndt/gfz103.sp400 ◽

2019 ◽

Vol 34 (Supplement_1) ◽

Author(s):

Tania Monzon ◽

Francisco Valga ◽

Magnolia Fleitas ◽

Ana Yurena Sanchez ◽

Noa Diaz ◽

...

Keyword(s):

Multicenter Study ◽

Hemodialysis Patients ◽

Cross Sectional ◽

Anthropometric Indexes

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Possible Early Vertical Transmission of COVID-19 from an Infected Pregnant Female to Her Neonate: A Case Report

Journal of Tropical Pediatrics ◽

10.1093/tropej/fmaa094 ◽

2020 ◽

Author(s):

Tapas Bandyopadhyay ◽

Abhinav Sharma ◽

Pratima Kumari ◽

Arti Maria ◽

Rahul Choudhary

Keyword(s):

Public Health ◽

Vertical Transmission ◽

Antibody Test ◽

Public Health Emergency ◽

Pregnant Female ◽

Rare Entity ◽

Rt Pcr ◽

Igg Antibody ◽

Mode Of Transmission ◽

Reported Data

Abstract Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-19) has emerged as a public health emergency in recent times. The reported data on the mode of transmission of coronavirus disease 2019 (COVID-19) are largely through contact, droplet, airborne and fomite transmission methods with vertical transmission being a rare entity. We hereby report a case of a probable vertical transmission of SARS-CoV-19 from an infected pregnant female to her neonate. The transmission has been confirmed by a positive RT-PCR at 16 h of life along with a positive IgG antibody test for SARS-CoV-19 in the baby and after excluding the possible environmental contamination of the sample. The baby was asymptomatic during the course of hospital stay and was discharged from the facility on Day 9 of life.

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The role of Chlamydia pneumoniae in severe acute tonsillitis

The Journal of Laryngology & Otology ◽

10.1017/s002221510012609x ◽

1994 ◽

Vol 108 (2) ◽

pp. 135-137 ◽

Cited By ~ 4

Author(s):

S. W. Hone ◽

J. Moore ◽

J. Fenton ◽

P. K. Gormley ◽

R. Hone

Keyword(s):

Chlamydia Pneumoniae ◽

Antibody Test ◽

Control Group ◽

Direct Immunofluorescence ◽

Serological Evidence ◽

Igg Antibody ◽

Acute Tonsillitis ◽

Recent Infection ◽

College Hospital

AbstractChlamydia pneumoniae has been implicated as a cause of tonsillitis and pharyngitis, but the incidence has varied from one to 19 per cent in various studies. We investigated 51 patients admitted to University College Hospital, Galway, with severe tonsillitis. Throat swabs were examined for evidence of Chlamydia pneumoniae using a direct monoclonal antibody test. Blood was taken for serology from 45 patients. A further specimen was taken at six weeks. A control group of 32 blood bank sera was used. Mean hospital stay was three days (one to eight). Five patients (10 per cent) were monospot positive. Chlamydia pneumoniae was identified by direct immunofluorescence on a tonsillar swab from one patient who did not seroconvert. IgG antibody was identified in 13 cases (29 per cent) and in seven of the control group (22 per cent). No serological evidence of recent infection was found. Chlamydia pneumoniae was not found to be a cause of severe acute tonsillitis in our study group.

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