scholarly journals Phytochemical analysis and antimicrobial and antioxidant activities of Celosia argentea leaves

2021 ◽  
Vol 17 (9) ◽  
Author(s):  
Genésio José da Silva Neto ◽  
Eronildo Joaquim de Santana ◽  
Amanda Reges de Sena ◽  
Bruno Anderson de Morais ◽  
Maria Isabela Ferreira de Araújo ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Micrococcus Luteus ◽  
Reducing Power ◽  
Spectrophotometric Analysis ◽  
Phytochemical Analysis ◽  
Antioxidant Power ◽  
Diffusion Technique ◽  
Celosia Argentea ◽  
Total Antioxidant ◽  
Layer Chromatography

This study performed the phytochemical screening and bioactivity of Celosia argentea leaves. The phytochemical profile of hexane (Hex), ethyl acetate (AcOEt) and methanolic (MeOH) extracts of C. argentea leaves was observed by Thin Layer Chromatography and spectrophotometric analysis. In the susceptibility test, the disk-diffusion technique was performed. Minimum Inhibitory Concentration (MIC) was assessed by microdilution. Minimum Microbic Concentration (MMC) was determined in the extracts which presented MIC. Antioxidant activities were measured using 2,2-diphenyl-1-picril-hidrazil (DPPH), determination of reducing power and total antioxidant capacity (TAC). The results showed a higher content of phenolic compounds (252.02 ± 0.02 mg GAE/g) in the extract AcOEt, and of tannins (103.72 ± 0.004 mg/EAT/g) in MeOH; this was effective against Micrococcus luteus (inhibition of 24.7 ± 0.6 mm). MIC and CMM of the AcOEt for M. luteus were 0.06 and 0.25 mg/mL, respectively. The MeOH extract eliminated 70.4 ± 0.03% of DPPH radical, whereas AcOEt had greater reducing power (289.23 ± 0.05 mg AA/g) and TAC at a concentration of 250 µg/mL (89.6%). This study revealed that C. argentea has phytochemicals with bactericidal and fungicidal potential, in addition to elevated antioxidant power. Key words: antimicrobial, antioxidant, bioactivity.

scholarly journals Phytochemical analysis and antimicrobial and antioxidant activities of Hen-riettea succosa (Aubl.) DC. leaves

2021 ◽  
Vol 9 (4) ◽  
pp. 340-349
Author(s):  
Izaqueu Rodrigues Da Silva ◽  
Bruno Anderson de Morais ◽  
Maria Isabela Ferreira De Araújo ◽  
Paula Perazzo de Souza Barbosa ◽  
Amanda Reges de Sena ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Micrococcus Luteus ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Spectrophotometric Analysis ◽  
Aureus Strain ◽  
Phytochemical Analysis ◽  
Meoh Extract ◽  
Minimum Concentration

Henriettea succosa is a tree species consumed in abundance by birds, however, there is no report on its phytochemical profile and biological activity. This study performed the phytochemical screening and the antimicrobial and antioxidant potential of H. succosa leaves. The hexane (Hex), ethyl acetate (AcOEt) and methanol (MeOH) extracts of the leaves were evaluated for chemical composition by Thin Layer Chromatography and spectrophotometric analysis; the antimicrobial activity was determined by the Minimum Inhibitory Concentration (MIC) and Minimum Microbicide Concentration (MMC); antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, determination of the reducing power and the phosphomolybdenum complex reduction assay. The photoprotor action of the extracts was also evaluated. The results showed a higher content of phenolic compounds (444.08 ± 0.020 mg EAG/g) and tannins (414.37 ± 0.16 mg EAG/g) in the MeOH extract, which was effective against Staphylococcus aureus and Serratia marcescens, with MIC of 1 mg/ ml and CMM of 2 mg/ml. The MIC and MMC of AcOEt for Micrococcus luteus was 1 mg/mL, this was also considered the minimum concentration necessary for the Hex extract to act on the S. aureus strain. The MeOH extract showed greater antioxidant activity by the DPPH (79.09%) and reducing power (327.2 ± 0.00 mg EAA/g) methods, while the AcOEt extract showed greater activity by the phosphomolybdenum method (40.5%). However, none of the extracts showed a photoprotective effect against UV radiation. In summary, this study revealed that the leaves of H. succosa have secondary metabolites with bactericidal potential, in addition to antioxidant action.

scholarly journals PHYTOCHEMICAL SCREENING AND EVALUATION OF LEAF PARTS OF HYPOESTES PHYLLOSTACHYA ROSEA FOR IN VITRO ANTIOXIDANT ACTIVITIES

2020 ◽  
pp. 54-58
Author(s):  
JENSON JACOB ◽  
MEENU JOHN
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Standard Procedure ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Soxhlet Apparatus ◽  
Total Antioxidant ◽  
Phytochemical Compounds ◽  
Reducing Power Assay

Objective: The aim of this study was focussed on phytochemical analysis, in vitro antioxidant activities of different solvent extracts of Hypoestes phyllostachya leaf. Methods: The leaves were washed efficiently, shade dried and coarsely powdered. The powdered leaf material of Hypoestes phyllostachya was successively extracted with hexane, chloroform, methanol and water using Soxhlet apparatus. Preliminary phytochemical screenings for polyphenolics, proteins, phytosteroids, flavonoids, tannins, glycosides, alkaloids carbohydrates, polyphenolics, saponins were done by following standard procedure. In vitro antioxidant activities of various solvent extracts were assessed using DPPH, ABTS, Hydroxy radical, total antioxidant capacity and reducing power assay. Polyphenolics and flavonoids were also estimated. Results: The results showed that most of the phytochemical compounds such as carbohydrates, proteins, steroids, fixed oils and fatty acids, flavonoids, alkaloids, saponins, tannins, polyphenolics and glycosides present in the methanolic and aqueous extract of Hypoestes phyllostachya. The in vitro antioxidant activity of Hypoestes phyllostachya leaf clearly showed that it has better antioxidant activity, which was confirmed by different assays. Conclusion: From this work, it can be concluded that Hypoestes phyllostachya has the potential to be a good antioxidant but have only a lesser amount of flavonoids and polyphenolic contents. This was the first report on the pharmacological activity of the Hypoestes phyllostachya.

scholarly journals Phytochemistry, Antioxidant Activity and Heavy Metal Content on Precooked and Raw of Celosia argentea L., Launaea taraxacifolia Wild. and Ocimum gratissimum L., Three Leafy Vegetables Eaten in Southern Benin

2019 ◽  
Vol 8 (1) ◽  
pp. 71
Author(s):  
Kizito Thimoléon AGBESSY ◽  
Camel Lagnika ◽  
Abdou Madjid O. Amoussa ◽  
John Nsor-Atindana ◽  
Ambaliou SANNI ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Leafy Vegetables ◽  
World Health ◽  
Phytochemical Analysis ◽  
Antioxidant Power ◽  
Ocimum Gratissimum ◽  
Celosia Argentea

This study aimed to estimate and compare the antioxidant potential as well as the levels of heavy metal contamination of Celosia argentea (Amaranthaceae), Launaea taraxacifolia (Asteracea) and Ocimum gratissimum (Lamiaceae) collected in four municipalities of South-Benin by taking into accounts the treatment prior to consumption (precooked and raw). A low extraction yield was obtained during the extraction of leafy vegetables subjected to precooking. The phytochemical screening of Launaea taraxacifolia revealed the presence of triterpenes, steroids, flavonoids and tannins. The phytochemical analysis of Ocimum gratissimum revealed the presence of triterpenes, the steroids, tannins, anthraquinones, coumarins and alkaloids. The analysis of Celosia argentea revealed the presence of triterpenes, steroids, flavonoids, tannins, anthraquinones and alkaloids. The antioxidant activity of the various extracts was found to be dose-dependent. The extracts showed significant antioxidant activities with IC50 ranging from 4.03 to 11.42 µg/ml. Ocimum gratissimum had the strongest antioxidant power followed by Celosia argentea and Launaea taraxacifolia respectively. Pre-cooking of leafy vegetables would have an effect on antioxidant activity, which resulted in a slight increase in the DPPH inhibition power of pre-cooked vegetables compared to raw one. The analysis of heavy metals showed the presence of arsenic, mercury and manganese in the different plant extracts. The highest levels were observed in leafy vegetables collect at Cotonou (Houeyiho) followed by those from Sèmè-Podji but all below the thresholds defined by the World Health Organization. The precooking promoted a decrease of the quantity in heavy metals by leaching, causing low concentration in metals of vegetables precooked in contrast with raw ones.

scholarly journals Phytochemical analysis, mineral composition and in vitro antioxidant activities of Celosia argentea leaves

2016 ◽  
Vol 4 (1) ◽  
pp. 19
Author(s):  
Usunomena Usunobun ◽  
Ekpemupolo I. Samuel
Keyword(s):  
Mineral Composition ◽  
Therapeutic Potential ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Mineral Analysis ◽  
Celosia Argentea

Background: Celosia argentea, a traditional vegetable in West and Central Africa is widely grown for ornamental purpose in the tropics and subtropics such as in Malaysia. The aim of this study is to determine phytochemicals, mineral composition and in vitro antioxidant activities of Celosia argentea leaves.Methods: Qualitative phytochemical screening was carried out using standard procedures, Mineral analysis was carried out using Atomic Absorption Spectrophotometer (AAS) while antioxidant potential, free radical scavenging and reducing power scavenging activity were also carried out.Results: The phytochemical composition revealed the presence of active ingredients such as glycosides, steroids, Saponins, Phenols, Flavonoids, and alkaloids while mineral analysis showed higher concentration in calcium (295mg/100g) and least concentration in chromium (0.85mg/100g). Other minerals and their concentrations includes magnesium (122.53mg/100g), potassium (128.33mg/100g), sodium (71.32mg/100g), iron (35.16mg/100g), zinc (5.42mg/100g), copper (2.18mg/100g), manganese (1.86mg/100g). Moreover, DPPH (2, 2-diphenyl-1-picrylhydrazyl) scavenging activity and reducing power activity of Celosia argentea increased as the concentration increases.Conclusion: The present study reveals therapeutic potential of Celosia argentea leaves due to its bioactive compounds and minerals. Also, Celosia argentea possess powerful antioxidant activity and can therefore offer good protection against oxidative damage.

scholarly journals In vitro Anticoccidial, Antioxidant Activities and Biochemical Screening of Methanolic and Aqueous Leaves Extracts of Selected Plants

2021 ◽  
Vol 41 (01) ◽  
pp. 57-63
Author(s):  
Wajiha .
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Eimeria Species ◽  
Reducing Power ◽  
Antioxidant Compounds ◽  
Biochemical Screening ◽  
Antioxidant Power ◽  
Methanolic Extracts ◽  
Total Antioxidant

Avian coccidiosis, a protozoan parasitic disease caused by genus Eimeria. Due to emergence of drug resistant Eimeria species, this study was aimed to evaluate anticoccidial potentials of Ficus racemosa, Cassia fistula and Syzygium cumini leaves extracts. In vitro anticoccidial efficacy of extracts was evaluated by oocysts sporulation inhibition and sporozoites viability inhibition assays of mixed Eimeria species oocysts. The set up was examined after 48hrs of incubation. DPPH radical scavenging activity, ferric reducing antioxidant power and total antioxidant capacity were used for the evaluation of antioxidant potential of extracts. Among tested extracts maximum oocysts sporulation inhibition 86.81±2.35% and sporozoites viability inhibition was 86.73±1.67% at concentration 30 mg/ml of C. fistula methanolic leaves extract against E. mitis and E. tenella respectively. Highest radical scavenging capacity 67.82±0.00 and reducing power 2.17±0.01 was shown by F. racemosa and C. fistula methanolic leaves extract respectively. Maximum total antioxidant power was observed in C. fistula 30.95±0.35 and F. racemosa 21.93±1.41µg/mg methanolic leaves extracts. Antioxidant compounds including phenols, flavonoids, alkaloids, saponins, carbohydrates etc. were detected through biochemical screening of selected plants extracts. The maximum amount of phenols 32.50±0.00µg/ml and flavonoids 40.00±1.00µg/ml were recorded in C. fistula methanolic extracts. It is concluded that selected plants methanolic extracts possess best anticoccidial and antioxidant activities due to presence of medicinally important phytochemicals. Further research is needed for identification and isolation of anticoccidial active compounds from these plants that can be used in the formulation of drugs against coccidiosis

scholarly journals Phytochemicals, Antioxidant and Antidiabetic Activities of Extracts from Miliusa velutina Flowers

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 555
Author(s):  
Vo Thi Tu Anh ◽  
Dai Thi Xuan Trang ◽  
Kaeko Kamei ◽  
Tran Chi Linh ◽  
Nguyen Huan Pham-Khanh ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Inhibitory Effect ◽  
Chemical Components ◽  
Antioxidant Power ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power ◽  
Reducing Power Assay

The flowers of M. velutina were extracted with ethanol to obtain a crude extract that was consecutively extracted using n-hexane, dichloromethane, ethyl acetate and water. The crude extract and fractions were studied for the chemical composition and antioxidant and antidiabetic activities. The extracts had various phytoconstituents, namely steroids, flavonoids, tannins, saponins, alkaloids and glycosides. The aqueous extract had the highest total polyphenol (12.6 mg GAE/g extract) and total flavonoid (205.6 mg QE/g extract) content. The aqueous extract exhibited the strongest antioxidant activities in the ferric reducing antioxidant power assay (EC50 = 4.0 µg/mL), reducing power assay (EC50 = 78.1 µg/mL), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid radical cation assay (EC50 = 48.2 µg/mL), total antioxidant capacity assay (EC50 = 8.7 µg/mL) and 1,1-diphenyl-2-picrylhydrazyl assay (EC50 = 9.3 µg/mL). The aqueous extract showed the strongest inhibitory effect on the activity of α-amylase (IC50 = 376.6 μg/mL) and α-glucosidase (IC50 = 69.7 μg/mL). The results showed that the aqueous extract of M. velutina flowers can be a promising candidate for the control of diabetes and oxidative stress. This is the first report about the chemical components and antioxidant and antidiabetic activities of M. velutina flower extracts.

scholarly journals Comparative Study on Phytochemical Profile and Antioxidant Activity of an Epiphyte, Viscum album L. (White Berry Mistletoe), Derived from Different Host Trees

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1191
Author(s):  
Mahak Majeed ◽  
Tanveer Bilal Pirzadah ◽  
Manzoor Ahmad Mir ◽  
Khalid Rehman Hakeem ◽  
Hesham F. Alharby ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Pearson Correlation ◽  
Juglans Regia ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Viscum Album ◽  
Total Phenol Content ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

The study aimed at evaluating the antioxidant profile of a medicinal epiphyte Viscum album L. harvested from three tree species, namely, Populus ciliata L, Ulmus villosa L., and Juglans regia L. The crude extracts were obtained with ethanol, methanol, and water and were evaluated for the total phenol content (TPC), total flavonoid content (TFC), and antioxidant activities using total reducing power (TRP), ferric reducing antioxidant power (FRAP), 1, 1-diphenyl 1-2-picryl-hydrazyl (DPPH), superoxide radical scavenging (SOR), and hydroxyl radical scavenging (•OH) assays. Our results showed that crude leaf extracts of plants harvested from the host Juglans regia L. exhibited higher yields of phytochemical constituents and noticeable antioxidative properties. The ethanolic leaf samples reported the highest phenols (13.46 ± 0.87 mg/g), flavonoids (2.38 ± 0.04 mg/g), FRAP (500.63 ± 12.58 μM Fe II/g DW), and DPPH (87.26% ± 0.30 mg/mL). Moreover, the highest values for TRP (4.24 ± 0.26 μg/mL), SOR (89.79% ± 0.73 mg/mL), and OH (67.16% ± 1.15 mg/mL) were obtained from aqueous leaf extracts. Further, Pearson correlation was used for quantifying the relationship between TPC, TFC, and antioxidant (FRAP, DPPH, SOR, OH) activities in Viscum album L. compared to their hosts. It was revealed that the epiphyte showed variation with the type of host plant and extracting solvent.

scholarly journals Phytochemical Screening, Polyphenols Content, Antioxidant Power, and Antibacterial Activity of Herniaria hirsuta from Morocco

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Kenza Ammor ◽  
Dalila Bousta ◽  
Sanae Jennan ◽  
Bahia Bennani ◽  
Abdellah Chaqroune ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Antioxidant Power ◽  
Negative Effect ◽  
Hydroethanolic Extract

The aim of this study is to investigate in vitro antioxidant and antibacterial activities of the aqueous and hydroethanolic extracts for aerial parts of Herniaria hirsuta. Extracts were screened for their possible antioxidant activities by three tests: DPPH free radical-scavenging, reducing power, and molybdenum systems. The screening of antibacterial activity of extracts was individually evaluated against sixteen bacteria species using a disc diffusion method. Flavonoids, total phenols, and tannins content were performed for both extracts. It shows higher content in the hydroethanolic extract. The hydroethanolic extract showed a significant antioxidant activity for the three methods studies to the aqueous extract, but nonsignificant results compared to the reference (BHT). However, both extracts have negative effect on the strains studies for the antibacterial activity.

scholarly journals PRELIMINARY SCREENING OF PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT, AND ANTI-BACTERIAL ACTIVITIES IN THE METHANOLIC LEAF EXTRACT OF MELIOSMA SIMPLICIFOLIA (L.)

2021 ◽  
pp. 100-104
Author(s):  
PAVITHRA S ◽  
SEKAR T
Keyword(s):  
Leaf Extract ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Hot Water ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Dpph Scavenging

Objective: In the present study is investigated of phytochemicals and antioxidant activities of the leaf extracts from Meliosma simplicifolia (L.). Methods: The seaweed sample was subjected to extraction with solvents of different polarities (hot water, methanol, acetone, chloroform, and petroleum ether) and screened for phytochemicals according to standard methods. The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, and superoxide radical (O2•) scavenging activity. The ICPMS and GC-MS analysis of the methanol leaf extract of M. simplicifolia was revealed the presence of antibacterial. Results: Phytochemical analysis revealed the presence of Preliminary metabolites Quantitative studies of estimated phenol, flavonoid and tannin, as for the methanol extract of stem showed the highest content of phenolic compounds (40.71±0.94 GAE mg/100). Antioxidant activities were concluded the estimation M. simplicifolia leaf for as followed the studies. In leaf the highest DPPH scavenging activity (132.3 μg/ml), ICP-MS analysis of the leaf extract showed the presence minerals such as Mg, Fe, Cu, Na, and Zn in excess. The leaf extract of the plant was also tested for its antibacterial activity and results confirmed that it has potential activity. Conclusion: The preliminary studies in the methanol extract of the leaf of M. simplicifolia are suggestive of the antibacterial potentials of the methanol extract of leaves of M. simplicifolia.

scholarly journals Antimicrobial and Antioxidant Activities of Different Extracts from Different Parts of Zilla spinosa (L.) Prantl

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Mohamed H. A. Suleiman ◽  
Ali A. Ateeg
Keyword(s):  
Traditional Medicine ◽  
Aerial Part ◽  
Antioxidant Activities ◽  
Shigella Flexneri ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Agar Disc

Zilla spinosa is commonly used in traditional medicine to treat gastrointestinal disorders and diabetes. In this study, aqueous ethanol (AE) and aqueous methanol (AM) extracts from aerial parts and roots of Z. spinosa were investigated. The total phenolic (TPC) and flavonoid (TFC) contents and antioxidant capacities in terms of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging, hydrogen peroxide (H2O2), and ferric reducing antioxidant power (FRAP) assays were determined, and the correlations among the results were assessed using Pearson’s correlation. The antimicrobial activity was assessed through agar disc diffusion and broth microdilution assays. Phytochemical screening showed that Z. spinosa extracts had alkaloids, glycosides, saponins, triterpenoids, phenols, and flavonoids in different abundances. The aerial part-AE extract contained low TPC (30.17 ± 4.24 mg GAE/g) and TFC (7.40 ± 1.02 mg QE/g) and displayed significant antioxidant capacity in the DPPH (IC50 = 52.17 ± 7.30 μg/mL), H2O2 (91.22 ± 2.60 μg/mL), and FRAP (EC50 = 98.70 ± 2.21 μg/mL) assays. By contrast, the root-AM extract contained high amounts of TPC (87.72 ± 7.75 mg GAE/g) and TFC (25.60 ± 1.57 mg QE/g). It showed significantly high antioxidant activity with IC50 values of 12.33 ± 1.88 μg/mL in the DPPH and 39.37 ± 2.59 μg/mL in the H2O2 assays, as well as reducing power capacity with an EC50 value of 20.82 ± 1.14 μg/mL in the FRAP assay. Both TPC and TFC were exhibited negative correlations ( p < 0.01 ) with the IC50 and EC50 values obtained in the applied antioxidant assays. The aerial part-AM extract showed the highest inhibitory activity against Staphylococcus aureus (26.5 ± 0.20 mm), followed by Shigella flexneri (19.4 ± 0.40 mm) and Proteus mirabilis (17.7 ± 0.49 mm). S. aureus was the most affected microorganism with a minimum inhibitory concentration of 128 μg/mL against the aerial part-AM extract. Interestingly, all evaluated extracts showed potent antifungal activity against Candida albicans. However, aerial part-AM was the most effective, with an inhibition zone of 12.6 ± 0.17 mm. The results concluded that Z. spinosa possesses different phytochemicals displaying significant antioxidant and antimicrobial activities, thus lending credence to its use in traditional medicine.

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