Dirofilaria repens infection as a cause of intensive peripheral microfilariemia in a Polish patient: process description and cases review

2018 ◽  
Vol 63 (3) ◽  
pp. 657-663 ◽  
Author(s):  
Matylda Kłudkowska ◽  
Łukasz Pielok ◽  
Krystyna Frąckowiak ◽  
Aleksander Masny ◽  
Elżbieta Gołąb ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Molecular Level ◽  
Gravid Female ◽  
Dirofilaria Repens ◽  
Parasitic Disease ◽  
Species Determination ◽  
Concentration Technique ◽  
Full Life Cycle ◽  
Morphological Findings ◽  
Polish Patient

Abstract Dirofilariasis is a parasitic disease of dogs and other carnivores transmitted mainly by the mosquitoes of the genera Culex, Aedes, Anopheles. Full life cycle of the Dirofilaria nematodes in humans is extremely rarely observed, usually lacking species determination at the molecular level. We report fully documented unusual clinical manifestation of subcutaneous dirofilariasis with intensive microfilariemia in peripheral blood revealed by the Knott’s concentration technique. The identification of the Dirofilaria repens nematode was based on typical morphological findings for adult gravid female nematode found in the histopathological preparations. The morphology of microfilariae obtained from patient’s peripheral blood was also typical for D. repens. The final identification was confirmed by the molecular analysis of microfilariae collected from the blood.

Concurrent subcutaneous and ocular infections with Dirofilaria repens in a polish patient: A case report in the light of epidemiological data

2021 ◽  
pp. 102481
Author(s):  
Beata Szostakowska ◽  
Agnieszka Ćwikłowska ◽  
Luiza Marek-Józefowicz ◽  
Artur Czaplewski ◽  
Dariusz Grzanka ◽  
...  
Keyword(s):  
Case Report ◽  
Epidemiological Data ◽  
Dirofilaria Repens ◽  
Ocular Infections ◽  
Polish Patient

scholarly journals Eosinophilic Gastroenteritis with a Relapsing and Remitting Course with Presence of Autoimmune Antibodies

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Chathuranga Lakmal Fonseka ◽  
Sunali Nanayakkara ◽  
S. D. A. L. Singhapura ◽  
H. M. M. Herath ◽  
C. K. Bodinayake
Keyword(s):  
Autoimmune Disease ◽  
Ascitic Fluid ◽  
Peripheral Blood ◽  
Elimination Diet ◽  
Eosinophilic Gastroenteritis ◽  
Normal Diet ◽  
Parasitic Disease ◽  
Case Presentation ◽  
Autoimmune Antibodies ◽  
Uncommon Disease

Background. Eosinophilic gastroenteritis (EGE) is an uncommon disease characterized by eosinophilic infiltration of the digestive tract, which occurs due to an uncertain aetiology. Although autoimmune diseases can later present as EGE, it is unusual for EGE to have positive autoimmune antibodies without the presence of an overt autoimmune disease. Case presentation. We report a 38-year-old previously healthy man who presented with abdominal discomfort and loose stools with pleural and peritoneal effusions progressing over several weeks. His investigations revealed severe eosinophilia in peripheral blood and ascitic fluid, and a laparoscopic full-thickness biopsy from the ileum demonstrated infiltration of eosinophils in all three layers of the intestine. There were no clinical features or investigations suggestive of parasitic disease, other diseases associated with eosinophilia, or autoimmune disease. Further investigations showed a highly positive ANA, positive p-ANCA, but did not meet the criteria to diagnose a specific autoimmune disease. The eosinophilia responded to an elimination diet with gradual resolution of eosinophilia and effusions, and once it reappeared after introduction of a normal diet. Conclusion. EGE presenting as peripheral blood and ascitic fluid eosinophilia with the presence of pleural and/or peritoneal effusions is uncommon. Eosinophilic gastroenteritis can be associated with autoantibody positivity without any evidence of overt autoimmune disease manifestations. Elimination diet can be used as a potential option to prevent recurrences of EGE.

scholarly journals Usual or unusual presentations of Dirofilaria repens in two sibling dogs: a case report

2020 ◽  
Author(s):  
Giulia Barlozzari ◽  
Tiziana Felice ◽  
Laura Salvato ◽  
Raffaella Conti ◽  
Claudio De Liberato ◽  
...  
Keyword(s):  
Gravid Female ◽  
Dirofilaria Repens ◽  
Tunica Vaginalis ◽  
Blood Samples ◽  
Subcutaneous Tissues ◽  
Gross Lesions ◽  
Classical Localization ◽  
Treatment Observation ◽  
Knott’S Test ◽  
Observation Period

Abstract This study describes two different manifestations of Dirofilaria repens infection in sibling dogs with microfilaremia. Dog 1, asymptomatic, harbored a gravid female of D. repens on the parietal layer of tunica vaginalis of one testicle and showed a marked circulating eosinophilia (3.3·103/μL). Both testicles were normal in shape and size without any gross lesions. Dog 2 had a pyotraumatic dermatitis. The cases were confirmed by PCR and sequencing. The sequences obtained showed 100% identity with those of D. repens isolated from human scrotum in Croatia. The treatment with moxidectin 2.5% and imidacloprid 10%/kg was effective in eliminating microfilariae after just one application, as demonstrated by negative modified Knott’s tests and PCR analyses of blood samples. This status was maintained during the post-treatment observation period. The classical localization of D. repens in dogs is in subcutaneous tissues, within nodules or free; however, it can also occur with some frequency in testicles, as described in humans. The infection can be associated with circulating eosinophilia or pyotraumatic dermatitis, as reported in this study. Thus, in endemic areas, it is advisable to carefully inspect the removed testicles at neutering since parasite localization can take place without any macroscopic changes. Moreover, in the case of circulating eosinophilia or pyotraumatic dermatitis, investigations should include modified Knott’s test and PCR to ensure that D. repens is not the cause of these alterations. Rapid and sensitive tests for the early detection of infected animals would help to prevent or limit the spread of this zoonosis.

scholarly journals Human Dirofilariasis repens - migratory cutaneous and visceral helminthiasis: new data of "the old" disease. Analysis of cases and review

2016 ◽  
Vol 21 (3) ◽  
pp. 157-165
Author(s):  
Alexander M. Bronshteyn ◽  
L. V Fedyanina ◽  
N. A Malyshev ◽  
N. G Kochergin ◽  
I. V Davydova ◽  
...  
Keyword(s):  
Clinical Presentation ◽  
Human Medicine ◽  
Dirofilaria Repens ◽  
Parasitic Disease ◽  
Human Being ◽  
Visceral Organs ◽  
Human Dirofilariasis ◽  
Pulmonary Dirofilariasis ◽  
Almost All ◽  
Disease Analysis

Dirofilaria repens is an emergent parasitic disease of dogs and man in Russiania. It infects humans accidentally via vectors. Occurrence D.repens in humans is most often in subcutaneous locations. Herein, we present autochtonous cases In Russiania of human dirofilariasis repens in almost all visceral organs with special attention to pulmonary dirofilariasis. Our data confirms the concept that the human being is a facultative host of Dirofilaria repens. Dirofilariasis prevalence is probably underestimated because of its non-specific clinical presentation. A national Russian prevalence study on dirofilariasis in animals and humans is called for in order to improve the management of the disease, both in veterinary and human medicine.

scholarly journals Theoretical and applied significance of systematics, its methodologies in the study of aphids (Homoptera, Aphidoidea)

2016 ◽  
pp. 316-339
Author(s):  
V.A. Mamontova
Keyword(s):  
Molecular Level ◽  
Phylogenetic Systematics ◽  
Species Determination ◽  
Genealogical Tree ◽  
Full Compliance ◽  
School Teaching ◽  
The Family ◽  
Main Thing ◽  
Evolutionary Taxonomy ◽  
Morphological Patterns

The literature data and the author’s own research, have exhaustively proved that taxonomy provides a framework for all other studies in entomology. The main thing in the taxonomy is full compliance with the genealogical tree of the study group, i.e., its evolutionary path determining the phylogeny of the group. There are two accepted methods for compiling the system and species determination: “evolutionary taxonomy”, which the author of the article is based on and economical computer Hennig’s “phylogenetic systematics” (Hennig, 1954, 1956) or cladistics. Specific examples show that the preference to cladistics among foreign aphidologists leads to by no means reliable conclusions, inconsistent with the systems under study. Even carried out at the high molecular level (Normark, 2000), it does not save the family tree from errors and conventions. Thus, cladistic methodology in the study of aphids (due to their complex biology, and polymorphism in particular) is completely unacceptable. Only evolutionary taxonomy, based on A. N. Severtsov’s and his school teaching «Morphological Patterns of Evolution» is allowable.

scholarly journals ASSOCIATION BETWEEN ATYPICAL DEPOLARIZATION IN CELLDYN 00 AND THE PRESENCE OF PLASMODIUM SPP IN BLOOD IN Dr. SOETOMO HOSPITAL SURABAYA

2015 ◽  
Vol 2 (1) ◽  
pp. 15
Author(s):  
Jusak Nugraha
Keyword(s):  
Peripheral Blood ◽  
Malaria Infection ◽  
Blood Smear ◽  
Screening Method ◽  
Peripheral Blood Smear ◽  
Accurate Method ◽  
High Morbidity ◽  
Parasitic Disease ◽  
Malaria Parasites ◽  
Cross Sectional

Background: Malaria is a parasitic disease worldwide with a high morbidity and mortality. A rapid and accurate method is needed to detect the presence of malaria parasites in blood. A flagging system atypical depolarization (atypdep) in CBC results from Cell-Dyn 3200 has been related with malaria infection. Materials and Methods: An observational cross sectional approach with 48 samples obtained from inpatients of the Dr.Soetomo Hospital, Surabaya. Samples were screened by Cell-Dyn 3200 analyzer for atypdep flagging in CBC. Positive samples were later confirmed by microscope to detect malaria parasites. results: From 48 samples with atypdep flagging, 7 samples were malaria positive on peripheral blood smear (13.1%). Most frequent atypdep flagging was seen in malignancy (18.7%), and approximately 54.6% of the samples were not accompanied by fever symptoms. Lekositosis and anemia each were found in 20 samples (41.6%) and thrombocytopenia in 33.3%. conclusion: The presence of atypdep flagging in Cell-Dyn 3200 does not necessarily indicate the existence of malaria or it could be said that atypdep flagging is not always associated with presence of malaria infection. The usage of an atypdep flagging in non-endemic areas such as Surabaya is just an alert sign to evaluate malaria infection rather than a screening method to detect malaria.

scholarly journals The "Morular Cell" and the "Grape Cell" in Bone Marrow and Peripheral Blood

Blood ◽  
1956 ◽  
Vol 11 (12) ◽  
pp. 1140-1147 ◽  
Author(s):  
AVINOAM ZLOTNICK
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Plasma Cells ◽  
Buffy Coat ◽  
Parasitic Disease ◽  
Reticulum Cells ◽  
Protein Nature ◽  
Grape Cell

Abstract Bone marrow aspirates of 39 patients with different diseases were examined. In 8 of these patients, who showed hyperglobulinemia of nonmyelomatous origin, three kinds of cells were found: 1) Plasmocytoid reticulum cells whose cytoplasm was filled with crystal-like configurations. 2) Plasmocytoid reticulum cells with hyaline transparent vesicles of different sizes in the cytoplasm. These cells resembled the "morular cell" of Mott. 3) Plasma cells the cytoplasm of which contained opaque bluish staining globular bodies varying in number and size. These cells were identical with the "grape cell." The "grape cell" was also found in the buffy coat of the blood from 2 patients with hyperglobulinemia, and the "morular cell" in the buffy coat from a patient suffering from an unusual parasitic disease. The protein nature of these inclusions is assumed, since they stained with the M.G.G. combination, but did not stain with Sudan black nor with P.A.S.

scholarly journals HUBUNGAN ANTARA FLAGGING ATYPDEP DI ALAT CELL-DYN 3200 DAN KEBERADAAN PLASMODIUM Spp DI DALAM DARAH PENDERITA DI RSUD DR.SOETOMO SURABAYA

2018 ◽  
Vol 17 (2) ◽  
pp. 97
Author(s):  
Esti Rohani ◽  
J Nugraha
Keyword(s):  
Peripheral Blood ◽  
Malaria Infection ◽  
Blood Smear ◽  
Malignant Disease ◽  
Screening Method ◽  
Peripheral Blood Smear ◽  
High Morbidity ◽  
Parasitic Disease ◽  
Malaria Parasites ◽  
Cross Sectional

Malaria is a parasitic disease worldwide with a high morbidity and mortality. A rapid and accurate methods is needed to detectthe presence of malaria parasites in blood. A flagging system atypical depolarization (atypdep) on CBC result from Cell-Dyn 3200instrument has been related with malaria infection. An observational cross sectional approach with a total of 48 samples were obtainedfrom inpatients in the Dr. Soetomo Hospital Surabaya. Samples were screened with Cell-Dyn 3200 analyzer for CBC found atypdepflagging. The positive samples were later confirmed by microscopic to detect malaria parasites. From 48 samples with atypdep flagging,seven samples were positive of malaria in peripheral blood smear (13.1%). Most frequent atypdep flagging was seen in malignant disease(18.7), an approximately 54.6% of the sample is not accompanied by symptoms of fever. Lekositosis and anemia were found in each of20 samples (41.6%) and thrombocytopenia in 33.3% of the samples. The presence of atypdep flagging does not necessarily indicate theexistence of malaria infection or it could be said that atypdep flagging is not always associated with the presence of malaria infection.The usage of an atypdep flagging on Cell-Dyn instrument in non-endemic areas such as Surabaya is just an alert sign to evaluate themalaria infection rather than a screening method to detect malaria.

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