scholarly journals PECAM-1 gene polymorphism (rs668) and subclinical markers of carotid atherosclerosis in patients with type 2 diabetes mellitus

2016 ◽  
Vol 19 (1) ◽  
pp. 63-70 ◽  
Author(s):  
D Popović ◽  
J Nikolajević Starčević ◽  
M Šantl Letonja ◽  
J Makuc ◽  
A Cokan Vujkovac ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Gene Polymorphism ◽  
Carotid Atherosclerosis ◽  
Control Group ◽  
Minor Effect ◽  
Initial Examination ◽  
Increased Risk

ABSTRACTThe platelet endothelial cell adhesion molecule 1 (PECAM-1) plays an important role in many inflammatory processes, including the development of atherosclerosis. Polymorphism rs668 of the PECAM-1 gene (373C/G) is functional, and it was reported to be associated with increased serum levels of PECAM-1. We investigated the association between the rs668 polymorphism of PECAM-1 and subclinical markers of carotid atherosclerosis in subjects with type 2 diabetes mellitus (T2DM). Five hundred and ninety-five T2DM subjects and 200 control subjects were enrolled. The carotid intima-media thickness (CIMT) and plaque characteristics (presence and structure) were assessed ultrasonographically. Biochemical analyses were performed using standard biochemical methods. Geno-typing of the PECAM-1 gene polymorphism (rs668) was performed using KASPar assays. The control examinations were performed 3.8 ± 0.5 years after the initial examination. Higher CIMT was found in patients with T2DM in comparison with subjects without T2DM. Statistically sig-nificantly faster progression of the atherosclerotic markers was shown in subjects with T2DM in comparison with the control group. When adjusted to other risk factors, the rs668 GG genotype was associated with an increased risk of carotid plaques in subjects with T2DM. We concluded that our study demonstrated a minor effect of the rs668 PECAM-1 on markers of carotid atherosclerosis in subjects with T2DM.

Matrix metalloproteinase-3 gene polymorphism (rs3025058) affects markers atherosclerosis in type 2 diabetes mellitus

VASA ◽  
2017 ◽  
Vol 46 (5) ◽  
pp. 363-369 ◽  
Author(s):  
Aleš Pleskovič ◽  
Marija Šantl Letonja ◽  
Andreja Cokan Vujkovac ◽  
Jovana Nikolajević Starčević ◽  
Martin Caprnda ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Gene Polymorphism ◽  
Matrix Metalloproteinase ◽  
Coronary Atherosclerosis ◽  
Carotid Atherosclerosis ◽  
Control Group

Abstract. Background: The study was designed to test the possible association between either polymorphisms of the matrix metalloproteinase-9 (MMP-9) gene (rs17576, rs3918242) or the MMP-3 5A/6A gene polymorphism (rs3025058) with markers of carotid atherosclerosis in patients with type 2 diabetes mellitus (T2DM). The second aim of the study was to demonstrate an association between either the rs17576, rs3918242 or rs3025058 and subclinical markers of coronary artery disease in the same subset of patients with T2DM. Patients and methods: A total of 595 subjects with T2DM and 200 subjects without T2DM (control group) were enrolled in the prospective study. Subclinical markers of carotid atherosclerosis were assessed ultrasonographically. Additionally, in a subset of subjects with T2DM a coronary computed tomography angiography (CCTA) was performed for diagnostic purposes. Genotyping of all three polymorphisms (rs17576, rs3918242, rs3025058) was performed with real-time PCR systems. Results: The comparison of atherosclerosis parameters was performed with regard to different genotypes of MMP-9 rs17576, rs3918242, and MMP-3 rs3025058 polymorphisms upon enrolment and during follow-up. In our study, we found an association between the MMP-3 rs3025058 and CIMT at the time of recruitment. Multiple linear regression analysis revealed the association of either the A- allele or the A- genotypes of the rs3025058 (MMP-3) with carotid intima media thickness (CIMT) progression in a 3.8-year follow-up. We demonstrated the effect of the rs3025058 on subclinical markers of coronary atherosclerosis (coronary calcium score, number of coronary arteries with more than 50 % stenosis, and presence of at least one vessel with more than 50 % stenosis). Conclusions: We found an association between the MMP-3 rs3025058 and subclinical markers of carotid (CIMT) and coronary atherosclerosis at the time of recruitment. Moreover, we demonstrated the effect of the MMP-3 rs3025058 on CIMT progression in the 3.8-year follow-up in patients with T2DM.

scholarly journals Nephrolithiasis against type 2 diabetes mellitus: on the effect of hypoglycemic therapy on lithogenesis

2018 ◽  
Vol 90 (10) ◽  
pp. 60-64
Author(s):  
S K Yarovoy ◽  
E N Kareva ◽  
O V Djalilov
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Negative Impact ◽  
Stone Formation ◽  
Hypoglycemic Agents ◽  
Control Group ◽  
City Hospital ◽  
Increased Risk

Aim. To study the effects of oral hypoglycemic agents that can affect the probability of recurrence of nephrolithiasis. Materials and methods. The article is based on the results of examination and treatment of 315 patients suffering from recurrent nephrolithiasis and medically compensated type 2 diabetes mellitus treated at the N.A. Lopatkin Institute of Urology and Interventional Radiology - the branch of the SMRC of Radiology, Ministry of Health of Russia and D.D. Pletnev City Hospital Moscow Healthcare Department in 2012-2017. The patients were divided into three groups according to the applied tool antidiabetic: metformin, glibenclamide, canagliflozin. The control group consisted of patients receiving insulin therapy. Results and discussion. The propensity of Metformin to reduce the pH of urine, which has a negative impact in the conditions of urate nephrolithiasis, which is most common in the population of patients with type 2 diabetes mellitus. Glibenclamide, on the contrary, somewhat latches urine. But changes in the reaction of urine under the influence of the drug do not go beyond normal values and are not clinically significant. Canagliflozin increases diuresis due to medication induced glycosuria and stimulates renal excretion of uric acid and its salts. However canagliflozin does not cause significant shifts in the pH of urine that may somewhat negates the increased risk of recurrence of urate stone formation in the background of the uricosuric effect of the drug. Conclusion. Drug therapy of type 2 diabetes mellitus significantly affects the properties of urine from patients with nephrolithiasis.

scholarly journals Vascular Endothelial Growth Factor Gene Polymorphism (rs2010963) and Its Receptor, Kinase Insert Domain-Containing Receptor Gene Polymorphism (rs2071559), and Markers of Carotid Atherosclerosis in Patients with Type 2 Diabetes Mellitus

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Sebastjan Merlo ◽  
Jovana Nikolajević Starčević ◽  
Sara Mankoč ◽  
Marija Šantl Letonja ◽  
Andreja Cokan Vujkovac ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Vascular Endothelial Growth Factor ◽  
Type 2 Diabetes Mellitus ◽  
Gene Polymorphism ◽  
Carotid Atherosclerosis ◽  
Vascular Endothelial ◽  
Receptor Kinase ◽  
Endothelial Growth Factor

Background. The current study was designed to reveal possible associations between the polymorphisms of the vascular endothelial growth factor (VEGF) gene (rs2010963) and its receptor, kinase insert domain-containing receptor (KDR) gene polymorphism (rs2071559), and markers of carotid atherosclerosis in patients with type 2 diabetes mellitus (T2DM).Patients and Methods. 595 T2DM subjects and 200 control subjects were enrolled. The carotid intima-media thickness (CIMT) and plaque characteristics (presence and structure) were assessed ultrasonographically. Biochemical analyses were performed using standard biochemical methods. Genotyping of VEGF/KDR polymorphisms (rs2010963, rs2071559) was performed using KASPar assays.Results. Genotype distributions and allele frequencies of the VEGF/KDR polymorphisms (rs2010963, rs2071559) were not statistically significantly different between diabetic patients and controls. In our study, we demonstrated an association between the rs2071559 of KDR and either CIMT or the sum of plaque thickness in subjects with T2DM. We did not, however, demonstrate any association between the tested polymorphism of VEGF (rs2010963) and either CIMT, the sum of plaque thickness, the number of involved segments, hsCRP, the presence of carotid plaques, or the presence of unstable carotid plaques.Conclusions. In the present study, we demonstrated minor effect of the rs2071559 of KDR on markers of carotid atherosclerosis in subjects with T2DM.

SLC30A8 gene polymorphism rs13266634 associated with increased risk for developing type 2 diabetes mellitus in Jordanian population

Gene ◽  
2021 ◽  
Vol 768 ◽  
pp. 145279
Author(s):  
Safaa Mashal ◽  
Mariam Khanfar ◽  
Sawsan Al-Khalayfa ◽  
Luma Srour ◽  
Lina Mustafa ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Gene Polymorphism ◽  
Jordanian Population ◽  
Increased Risk

scholarly journals Association between Toll-Like Receptor 4 and Occurrence of Type 2 Diabetes Mellitus Susceptible to Pulmonary Tuberculosis in Northeast China

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Yuze Li ◽  
Dianzhong Li ◽  
Jinfeng Zhang ◽  
Shurui Liu ◽  
Haijun Chen ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Pulmonary Tuberculosis ◽  
Gene Polymorphism ◽  
Future Research ◽  
Control Group ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4

The purpose of this study is to explore why type 2 diabetes mellitus (T2DM) patients are susceptible to pulmonary tuberculosis through detection of serum Toll-like receptor 4 (TLR4), an important immune-related receptor, especially in terms of content and TLR4gene polymorphism. Patients with T2DM complicated by pulmonary tuberculosis (T2DMTB) were selected as the case group and T2DM patients without tuberculosis were selected as the control group. Forty patients in each group were randomly selected and their serum TLR4levels were detected and compared. Determination of six sites of TLR4gene polymorphism was carried out in 238 T2DMTB patients and 310 patients with T2DM, and results showed that the serum TLR4content of the T2DMTB group was significantly lower than that of the T2DM group (p<0.05). The six sites of TLR4gene polymorphism did not show significant associations with T2DMTB risk. No statistically significant differences in genotype distributions were observed between T2DMTB patients and patients with T2DM when studied using the recessive and dominant genetic models. How two diseases with contradictory nutritional statuses can occur in the same person is difficult to explain from environmental factors perspective alone. Future research should study the causes of T2DMTB from the perspective of genetics.

scholarly journals Chemerin rs17173608 Gene Polymorphism is not Associated with Type 2 Diabetes Mellitus: a Cross-sectional Study

Folia Medica ◽  
2019 ◽  
Vol 61 (1) ◽  
pp. 69-75
Author(s):  
Serdar Olt ◽  
Orhan Öznas ◽  
Haydar Bağış ◽  
Eda Tahir Turanlı
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Gene Polymorphism ◽  
Healthy Control Group ◽  
Control Group ◽  
Arms Pcr ◽  
Significant Difference ◽  
Healthy Control

Abstract Background: Previous studies have shown that chemerin has important roles in the development of obesity, insulin resistance, metabolic syndrome, polycystic ovary syndrome (PCOS) and T2DM. The main goal of our study was to investigate the role of Chemerin rs17173608 gene polymorphism in T2DM (type 2 diabetes mellitus). Materials and methods: 100 patients with T2DM and 50 healthy volunteers were included in the present study. DNA isolation from blood samples was performed with K1820-02 DNA Mini Kit. Chemerin gene polymorphism was detected by Tetra- Amplification Refractory mutation system polymerase chain reaction (T-ARMS-PCR). At the end of T-ARMS-PCR, samples were run using gel electrophoresis. Some samples were validated by sequence analysis. Results: In the genotype analysis, 18.0% of patients had TT genotype and 81.0% of TG genotype was detected. GG genotype was not detected in any patient. Genotype of 1 patient was unidentified. Genotype distribution of healthy control group was 12.0% TT genotype and 88.0% TG genotype. Similar to the T2DM group, the GG genotype was not detected in the control group. There was no statistically significant difference between T2DM group and healthy control group for TG and TT genotypes. Conclusion: To our knowledge, chemerin rs17173608 gene polymorphism has been investigated in T2DM for the first time herein. In the present study, the TT genotype ratios were higher in the T2DM subjects than in healthy subjects. G allele frequency in the T2DM group was lower than that in the control group. However, there was no statistically significant difference between the groups.

scholarly journals The Risk Factors of Severe Hypoglycemia in Older Patients with Dementia and Type 2 Diabetes Mellitus

2022 ◽  
Vol 12 (1) ◽  
pp. 67
Author(s):  
Nai-Ching Chen ◽  
Chien-Liang Chen ◽  
Feng-Chih Shen
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factors ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Glycemic Control ◽  
Older Patients ◽  
Control Group ◽  
Research Database ◽  
Increased Risk

Background: The adequate glycemic control and risk factors for hypoglycemia in older patients with dementia and type 2 diabetes mellitus (T2DM) remain unclear. This study aimed to analyze the status of glycemic control and determine the risk of hypoglycemia among these groups. Methods: A hospital admission record due to hypoglycemia through an emergency room with glucose supplementation in the Chang Gung Memorial Hospital was identified as a hypoglycemic event. Patients with dementia and T2DM without hypoglycemic events throughout the study period were defined as the control group. We gathered patients aged ≥65 years with a diagnosis of Alzheimer’s dementia (AD) and T2DM between 2001 and 2018 in the Chang Gung Research Database (CGRD). We extracted data included medication use, diagnoses, and biochemistry data from hospital records. Results: A total of 3877 older patients with dementia and T2DM with regular visits to the outpatient department were enrolled in this study. During the two-year follow-up period, 494 participants (12.7%) experienced hypoglycemia. Multivariable logistic multivariable regression models for hypoglycemic events showed that metformin had a protective effect (odds ratio (OR) = 0.75, p = 0.023), insulin had the highest risk (OR = 4.64, p < 0.001). Hemoglobin A1c (HbA1c) levels were not correlated with hypoglycemic events (OR = 0.95, p = 0.140). Patients with hypoglycemic episodes had a significantly higher proportion of ≥2 Charlson Comorbidity Index scores than those without hypoglycemic episodes (83.2% versus 56.4%, p < 0.001). Conclusions: Drug regimen affects hypoglycemic episodes but not HbA1c in older patients with dementia and T2DM. In addition, patients with more comorbidities experience an increased risk of hypoglycemia.

scholarly journals POLYMORPHISMS OF INSULIN RECEPTOR SUBSTRATE 1 AS A RISK FACTOR FOR TYPE 2 DIABETES MELLITUS, OBESITY AND CHRONIC PANCREATITIS AMONG POPULATION OF TERNOPIL REGION

2021 ◽  
Vol 6 (2) ◽  
pp. 30-36
Author(s):  
U. P. Hevko ◽  
M. I. Marushchak
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Chronic Pancreatitis ◽  
Gene Polymorphism ◽  
University Hospital ◽  
Gene Localization ◽  
Control Group ◽  
Hardy Weinberg Equilibrium

Background. The course of type 2 diabetes mellitus (T2DM), obesity and chronic pancreatitis (CP) in most cases is not isolated, so it requires broadening the knowledge about the pathogenetic links at their combined course. Despite significant advances in genome research, most of the genetic factors that cause development of T2DM are still unclear. Objective. The aim of the study was to establish the prevalence of IRS1 gene polymorphism in the patients with T2DM as well as obesity and CP. Methods. The study involved 34 patients with T2DM who were hospitalized in the endocrinology department of Ternopil University Hospital in 2019-2020 and 10 apparently healthy patients. Analysis of IRS1 gene polymorphism (SNP in the promoter region - rs2943640; gene localization 2q36.3) was performed on the basis of polymerase chain reaction data using specific primers. Results. It was found that the frequencies of the genotype responsible for C/A polymorphism of IRS1 gene in T2DM, T2DM with obesity and in the combined course of T2DM with obesity and CP did not deviate significantly from the Hardy-Weinberg equilibrium (p>0.05). The patients with combined course of T2DM, obesity and chronic pancreatitis experienced a probable influence of genotypes C/C and C/A of IRS1 gene on the development of the studied comorbidity (p<0.05), which is confirmed by a probable difference in the dominant model of IRS1 gene inheritance only when T2DM was combined with obesity and CP compared to the control group (p<0.001). Conclusions. The presence of the C allele in both homozygous and heterozygous states may increase the risk of T2DM comorbidity, obesity and CP in the population of Ternopil region.

scholarly journals PARAMETERS OF BONE METABOLISM IN PRE-AND POSTMENOPAUSAL WOMEN WITH TYPE 2 DIABETES MELLITUS IN THE RATIONALE FOR THE CHOICE OF ANTIOSTEOPOROTIC THERAPY

2013 ◽  
Vol 16 (3) ◽  
pp. 8-12
Author(s):  
L A Ruyatkina ◽  
A V Lomova ◽  
D S Ruyatkin ◽  
V V Romanov
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Bone Remodeling ◽  
Negative Relationship ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
C Peptide ◽  
Increased Risk

Introduction. Bone health in type 2 diabetes mellitus (T2DM) is discussed for a long time as a known fact for increased risk of fractures. Purpose. To estimate the parameters of bone remodeling in pre-andpostmenopausal women with T2DM. Materials and Methods. In a cross-sectional pilot study which included 80 women: 40 with T2DM and 40 without disturbances of glucose metabolism (K), divided into subgroups of premenopausal (pre) and natural menopause (post) — 20 patients each. Results and conclusions. We found a tendency toward a decrease in bone turnover in patients T2DM, a significant negative relationship with C-peptide and ALP in T2DM, a positive correlation with tartrate — resistant acid phosphatase in the control group, although the levels of C-peptide did not differ in patients with T2DM and in the control group . Identified characteristics of decreased bone remodeling in patients with T2DM pathogenically justify the use of anabolic antiosteoporotic drugs for treatment of osteoporosis in such patients.

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