scholarly journals Within-subject and between-subject biological variation estimates of 21 hematological parameters in 30 healthy subjects

2018 ◽  
Vol 56 (8) ◽  
pp. 1309-1318 ◽  
Author(s):  
Abdurrahman Coşkun ◽  
Anna Carobene ◽  
Meltem Kilercik ◽  
Mustafa Serteser ◽  
Sverre Sandberg ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Biological Variation ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Values ◽  
Performance Specifications ◽  
The Mean ◽  
Variance Homogeneity

Abstract Background: The complete blood count (CBC) is used to evaluate health status in the contexts of various clinical situations such as anemia, infection, inflammation, trauma, malignancies, etc. To ensure safe clinical application of the CBC, reliable biological variation (BV) data are required. The study aim was to define the BVs of CBC parameters employing a strict protocol. Methods: Blood samples, drawn from 30 healthy subjects (17 females, 13 males) once weekly for 10 weeks, were analyzed using a Sysmex XN 3000 instrument. The data were assessed for normality, trends, outliers and variance homogeneity prior to coefficient of variation (CV)-analysis of variance (ANOVA). Sex-stratified within-subject (CVI) and between-subjects (CVG) BV estimates were determined for 21 CBC parameters. Results: For leukocyte parameters, with the exception of lymphocytes and basophils, significant differences were found between female/male CVI estimates. The mean values of all erythrocyte-, reticulocyte- and platelet parameters differed significantly between the sexes, except for mean corpuscular hemoglobin concentration, mean corpuscular volume and platelet numbers. Most CVI and CVG estimates appear to be lower than those previously published. Conclusions: Our study, based on a rigorous protocol, provides updated and more stringent BV estimates for CBC parameters. Sex stratification of data is necessary when exploring the significance of changes in consecutive results and when setting analytical performance specifications.

scholarly journals Development and health status of Centropomus undecimalisparasitized by Rhabdosynochus rhabdosynochus (Monogenea) under different salinity and temperature conditions

2015 ◽  
Vol 24 (3) ◽  
pp. 350-356 ◽  
Author(s):  
Giovanni Lemos de Mello ◽  
Gabriela Tomas Jerônimo ◽  
Karen Roberta Tancredo ◽  
Jéssica Brol ◽  
Evelyn Jacques de Almeida ◽  
...  
Keyword(s):  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Economic Losses ◽  
Mean Corpuscular Hemoglobin ◽  
Experimental Conditions ◽  
Different Temperatures ◽  
The Mean ◽  
Positive Correlations ◽  
C 32 ◽  
Wbc Count

This study evaluated the correlation of hematological parameters with the mean abundance of the monogenean helminth Rhabdosynochus rhabdosynochus in Centropomus undecimalis reared at different temperatures and salinities. The experimental conditions were: 28 °C/0 ppt (parts per thousand); 28 °C/15 ppt; 28 °C/32 ppt; 25 °C/0 ppt; 25 °C/15 ppt; and 25 °C/32 ppt. The prevalence was 100.0% in fish at 28 °C/15 ppt, 28 °C/32 ppt and 25 °C/15 ppt, which was significantly different (p < 0.05) from those at 25 °C/32 ppt. The red blood cell (RBC) count, hematocrit and total leukocyte (WBC) count were significantly higher in fish at 28 °C/15 ppt and 28 °C/32 ppt. The mean abundance of R. rhabdosynochus, hematocrit and RBC showed positive correlations (P < 0.05) with temperature (ρ= 0.3908; ρ= 0.4771 and ρ = 0.2812). Mean abundance showed negative correlations with hemoglobin (ρ= -0.3567) and mean corpuscular hemoglobin concentration (MCHC) (ρ = -0.2684). No correlation between abundance and salinity was detected among the experimental conditions (ρ = -0.0204). The low numbers of monogeneans recorded (min -1 and max -33) explain the few changes to fish health. This suggests that these experimental conditions may be recommended for development of rearing of C. undecimalis in Brazil, without any influence or economic losses from R. rhabdosynochus.

scholarly journals Blood Donation in Nigeria: Standard of the Donated Blood

2012 ◽  
Vol 4 (02) ◽  
pp. 094-097 ◽  
Author(s):  
Nwogoh Benedict ◽  
Awodu Omolade Augustina ◽  
Bazuaye Godwin Nosakhare
Keyword(s):  
Blood Donation ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Mean Cell Volume ◽  
Benin City ◽  
Cell Counts ◽  
Significant Difference ◽  
The Mean

ABSTRACT Objective: The objective was to determine the basic hematological parameters of remunerated blood donors in Benin City and to compare them with those of voluntary donors. Materials and Methods: This is a prospective study conducted in a tertiary health facility in Benin City. Pretransfusion samples were obtained from blood bags after gentle mixing and analyzed for hematological parameters. Samples were analyzed using the hematology autoanalyzer MODEL SYSMEX KN21. Result: A total of 215 samples were obtained comprising 160 remunerated (paid) and 55 voluntary donor samples. In the paid donors, the mean hemoglobin concentration (Hb) and hematocrit (HCT) 7.7±2.9 and 28.8±8.5 respectively. This was significantly lower than those of voluntary donors who had 13.9±1.2 and 42.2±3.3 with P < 0.001. The mean values of the red cell counts (RBC), white cell counts (WBC), mean cell volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were significantly lower in paid donors as P-values were <0.001. MCV was significantly low but not compared to the other parameters as P=0.04. There was no significant difference in the platelet count. Conclusion: Paid donors in Benin City have significantly lower hematological parameters than controls.

scholarly journals Hematological parameters of Hoplias malabaricus (Characiformes: Erythrinidae) parasitized by Monogenea in lagoons in Pirassununga, Brazil

2013 ◽  
Vol 22 (4) ◽  
pp. 457-462 ◽  
Author(s):  
Lincoln Lima Corrêa ◽  
Letícia Cucolo Karling ◽  
Ricardo Massato Takemoto ◽  
Paulo Sérgio Ceccarelli ◽  
Marlene Tiduko Ueta
Keyword(s):  
Condition Factor ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Good Health ◽  
Blood Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Hoplias Malabaricus ◽  
Relative Condition Factor ◽  
The Mean

Hematology is an important pathological and diagnostic tool. This paper describes the hematological parameters of 76 specimens of Hoplias malabaricus infested with monogenean parasites, which were collected from two lagoons. The hematological parameters included: erythrocyte count (Er), microhematocrit (Hct), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC). The blood parameters showed no significant changes associated with the infestation. Water temperature was found to be negatively correlated with MCV and Hct, with values of rs= −0.52, p<0.0001 and rs= −0.48, p<0.0001, respectively. The mean Relative Condition Factor was Kn=1.01, indicating good health conditions of the fish in these lagoons. No correlation was found between the monogenean infestation and the blood parameters, or between the environmental factors and the monogeneans.

Long-term biological variation estimates of 13 hematological parameters in healthy Chinese subjects

2020 ◽  
Vol 58 (8) ◽  
pp. 1282-1290 ◽  
Author(s):  
Chenbin Li ◽  
Mingting Peng ◽  
Ji Wu ◽  
Zhongli Du ◽  
Hong Lu ◽  
...  
Keyword(s):  
Complete Blood Count ◽  
Clinical Chemistry ◽  
Hematological Parameters ◽  
Age Distribution ◽  
Turnover Time ◽  
Biological Variation ◽  
European Federation ◽  
Mean Values ◽  
Healthy Chinese ◽  
Chinese Subjects

AbstractBackgroundThe complete blood count (CBC) is a basic test routinely ordered by physicians as a part of initial diagnostic work-up on their patients. To ensure safe clinical application of the CBC, reliable biological variation (BV) data are needed to establish analytical performance specifications. Our aim was to define the BV of CBC parameters using a rigorous protocol that is compliant with the Biological Variation Data Critical Appraisal Checklist (BIVAC) provided by the European Federation of Clinical Chemistry and Laboratory Medicine.MethodsBlood samples drawn from 41 healthy Chinese subjects (22 females and 19 males; 23–59 years of age) once monthly for 6 consecutive months were analyzed using an ABX Pentra 80 instrument. The instrument was precisely calibrated. All samples were analyzed in duplicate for 13 CBC parameters. The data were assessed for outliers, normality, and variance homogeneity prior to nested ANOVA. Gender-stratified within-subject (CVI) and between-subject (CVG) BV estimates were calculated.ResultsThe number of remaining data for each subject was 442–484 after removing outliers. No significant differences existed between female/male CVI estimates. Except for leukocytes, neutrophils, and lymphocytes, the mean values of 10 parameters differed significantly between genders, rendering partitioning of CVG data between genders. No significant differences were detected between most BV estimates and recently published estimates representing a Europid population.ConclusionsMost BV estimates in BIVAC-compliant studies are similar. The turnover time of blood cells and age distribution of participants should be considered in a CBC BV study. Our study will contribute to global BV estimates and future studies.

Effect of gender on hematological parameters in growing Ouled Djellal sheep of semi arid zones of Northeastern Algeria

2021 ◽  
Vol 11 (4) ◽  
pp. 407-417
Author(s):  
Zerari Aicha ◽  
Mahdi Djahida ◽  
Allaoua Sofia Amel ◽  
Rouar Salim
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Arid Zones ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Mean Values ◽  
Ewe Lambs

Ouled Djellal sheep is the most dominant breed in Algeria due to its adapta-tion to harsh steppe environment. Hematological parameters are good indi-cators of animal health, but their values are breed-specific, and they are affected by age and gender. Therefore, this study aimed to determine the hematological profile of healthy Ouled Djellal lambs and ewe lambs reared traditionally under semi-arid zones of Algeria during the growing peri-od. Blood samples were taken from twenty-four Ouled Djellal lambs (12 males, 12 females) at 42, 63, 84 and 105 days of age to evaluate the count of White Blood Cells, Lymphocyte, Monocyte, Granulocyte, Red Blood Cell and total Platelet, the percentage of Lymphocyte, Monocyte and Granulocyte, Hemoglobin concentration, Hematocrit, Mean Corpuscular Volume, Mean Corpuscular Hemoglobin and Mean Corpuscular Hemoglobin Concentration by usual known methods. Both male and female lambs did not show signifi-cant variations in mean values of White Blood Cells, Lymphocyte count, Red Blood Cells, Hemoglobin, Mean Corpuscular Hemoglobin concentration, per-centage of Monocyte, Monocyte count, Gran percentage and Granulocyte count from 42ͭ ͪ day to 105ͭ ͪ days. Mean values of percentage of Lymphocyte, Hematocrite in lambs, Mean Corpuscular Hemoglobin in ewe lambs, and Mean Corpuscular Volume, Platelets levels in both lambs and ewe lambs indicated also variations during this period of growth. Significant Pearson's correlations were found between some hematological parameters in Ouled Djellal lambs and/or ewe lambs. Therefore, these findings provide usual val-ues, which can be considered for assessing the physiological, nutritional and health status of these animals.

scholarly journals Preliminary evaluation of complete blood count and diurnal variation of hematological parameters in black rat snake (Pantherophis obsoletus)

2020 ◽  
Vol 74 (1) ◽  
pp. 58-67 ◽  
Author(s):  
Maja Belic ◽  
Maja Lukac ◽  
Nika Dvojkovic ◽  
Tena Galesic ◽  
Mirna Robic ◽  
...  
Keyword(s):  
Blood Count ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Preliminary Evaluation ◽  
Corpuscular Hemoglobin ◽  
Black Rat ◽  
Black Rat Snakes ◽  
Rat Snake

Introduction. The purposes of this study were to determine the presence of daily variations in hematological parameters of the black rat snake (Pantherophis obsoletus) and to compare them with the reference interval values of its subspecies, yellow rat snake (Pantherophis obsoleta quadrivittata). Materials and Methods. Blood from four black rat snakes was collected in the morning and in the evening, and after each collection, blood smears, packed cell volume (PCV), red blood cell count (RBC) and hemoglobin concentration (HGB) were determined, while erythrocyte indices of mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were calculated and differential leukocyte counts were evaluated. Results and Conclusions. There were no statistically significant differences in any of the blood parameters in the morning and in the evening. Most of the complete blood count values in the black rat snakes were lower, but still within the reference range of those found in the literature for the yellow rat snake. The results of hematological parameters of the black rat snake could be beneficial for further research of physiological and pathological variations in complete blood count of these snakes, for diagnosing health conditions and for detecting various diseases.

scholarly journals European Biological Variation Study (EuBIVAS): within- and between-subject biological variation estimates for serum thyroid biomarkers based on weekly samplings from 91 healthy participants

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Michela Bottani ◽  
Aasne K. Aarsand ◽  
Giuseppe Banfi ◽  
Massimo Locatelli ◽  
Abdurrahman Coşkun ◽  
...  
Keyword(s):  
Large Scale ◽  
Thyroid Stimulating Hormone ◽  
Biological Variation ◽  
Healthy Individuals ◽  
Serum Samples ◽  
Free Triiodothyronine ◽  
Performance Specifications ◽  
Study Population ◽  
Variance Homogeneity ◽  
Healthy Participants

Abstract Objectives Thyroid biomarkers are fundamental for the diagnosis of thyroid disorders and for the monitoring and treatment of patients with these diseases. The knowledge of biological variation (BV) is important to define analytical performance specifications (APS) and reference change values (RCV). The aim of this study was to deliver BV estimates for thyroid stimulating hormone (TSH), free thyroxine (FT4), free triiodothyronine (FT3), thyroglobulin (TG), and calcitonin (CT). Methods Analyses were performed on serum samples obtained from the European Biological Variation Study population (91 healthy individuals from six European laboratories; 21–69 years) on the Roche Cobas e801 at the San Raffaele Hospital (Milan, Italy). All samples from each individual were evaluated in duplicate within a single run. The BV estimates with 95% CIs were obtained by CV-ANOVA, after analysis of variance homogeneity and outliers. Results The within-subject (CV I ) BV estimates were for TSH 17.7%, FT3 5.0%, FT4 4.8%, TG 10.3, and CT 13.0%, all significantly lower than those reported in the literature. No significant differences were observed for BV estimates between men and women. Conclusions The availability of updated, in the case of CT not previously published, BV estimates for thyroid markers based on the large scale EuBIVAS study allows for refined APS and associated RCV applicable in the diagnosis and management of thyroid and related diseases.

scholarly journals The use of a neck brace does not influence visual vertical perception

2011 ◽  
Vol 69 (3) ◽  
pp. 509-512 ◽  
Author(s):  
Martha Funabashi ◽  
Natya N.L. Silva ◽  
Luciana M. Watanabe ◽  
Taiza E.G Santos-Pontelli ◽  
José Fernando Colafêmina ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Healthy Subjects ◽  
Afferent Input ◽  
Subjective Visual Vertical ◽  
Sensory Receptors ◽  
Ocular Torsion ◽  
Vertical Orientation ◽  
Mean Values ◽  
Visual Vertical ◽  
The Mean

Subjective visual vertical (SVV) evaluates the individual's capacity to determine the vertical orientation. Using a neck brace (NB) allow volunteers' heads fixation to reduce cephalic tilt during the exam, preventing compensatory ocular torsion and erroneous influence on SVV result. OBJECTIVE: To analyze the influence of somatosensory inputs caused by a NB on the SVV. METHOD: Thirty healthy volunteers performed static and dynamic SVV: six measures with and six without the NB. RESULTS: The mean values for static SVV were -0.075º±1.15º without NB and -0.372º±1.21º with NB. For dynamic SVV in clockwise direction were 1.73º±2.31º without NB and 1.53º±1.80º with NB. For dynamic SVV in counterclockwise direction was -1.50º±2.44º without NB and -1.11º±2.46º with NB. Differences between measurements with and without the NB were not statistically significant. CONCLUSION: Although the neck has many sensory receptors, the use of a NB does not provide sufficient afferent input to change healthy subjects' perception of visual verticality.

scholarly journals Effects of food and sucralfate on a single oral dose of 500 milligrams of levofloxacin in healthy subjects.

1997 ◽  
Vol 41 (10) ◽  
pp. 2196-2200 ◽  
Author(s):  
L J Lee ◽  
B Hafkin ◽  
I D Lee ◽  
J Hoh ◽  
R Dix
Keyword(s):  
Oral Dose ◽  
Healthy Subjects ◽  
High Fat ◽  
Time Curve ◽  
Mean Values ◽  
Treatment Groups ◽  
The Mean ◽  
The Individual ◽  
Individual Profiles ◽  
Fat Meal

The effects of food and sucralfate on the pharmacokinetics of levofloxacin following the administration of a single 500-mg oral dose were investigated in a randomized, three-way crossover study with young healthy subjects (12 males and 12 females). Levofloxacin was administered under three conditions: fasting, fed (immediately after a standardized high-fat breakfast), and fasting with sucralfate given 2 h following the administration of levofloxacin. The concentrations of levofloxacin in plasma and urine were determined by high-pressure liquid chromatography. By noncompartmental methods, the maximum concentration of drug in serum (Cmax), the time to Cmax (Tmax), the area under the concentration-time curve (AUC), half-life (t1/2), clearance (CL/F), renal clearance (CLR), and cumulative amount of levofloxacin in urine (Ae) were estimated. The individual profiles of the drug concentration in plasma showed little difference among the three treatments. The only consistent effect of the coadministration of levofloxacin with a high-fat meal for most subjects was that levofloxacin absorption was delayed and Cmax was slightly reduced (Tmax, 1.0 and 2.0 h for fasting and fed conditions, respectively [P = 0.002]; Cmax, 5.9 +/- 1.3 and 5.1 +/- 0.9 microg/ml [90% confidence interval = 0.79 to 0.94] for fasting and fed conditions, respectively). Sucralfate, which was administered 2 h after the administration of levofloxacin, appeared to have no effect on levofloxacin's disposition compared with that under the fasting condition. Mean values of Cmax and AUC from time zero to infinity were 6.7 +/- 3.2 microg/ml and 47.9 +/- 8.4 microg x h/ml, respectively, following the administration of sucralfate compared to values of 5.9 +/- 1.3 microg/ml and 50.5 +/- 8.1 microg x h/ml, respectively, under fasting conditions. The mean t1/2, CL/F, CLR, and Ae values were similar among all three treatment groups. In conclusion, the absorption of levofloxacin was slightly delayed by food, although the overall bioavailability of levofloxacin following a high-fat meal was not altered. Finally, sucralfate did not alter the disposition of levofloxacin when sucralfate was given 2 h after the administration of the antibacterial agent, thus preventing a potential drug-drug interaction.

scholarly journals HEMATOLOGICAL CHARACTERIZATION OF BETA-THALASSEMIA IN SUDANESE PATIENTS

2020 ◽  
pp. 5-7
Author(s):  
RABAB HASSAN ELSHAIKH ◽  
SANAA ELFATIH HUSSEIN
Keyword(s):  
Iron Deficiency ◽  
Hemoglobin Concentration ◽  
Mean Value ◽  
Beta Thalassemia ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Significant Difference ◽  
Hematological Analysis ◽  
The Mean

Thalassemia is common inherited disorder among humans, and they represent a major public health problem in many areas of the world. The study aimed to the measurement of hematological characterization of beta-thalassemia in Sudanese patients. Blood samples from 61 beta-thalassemic patients were collected after written consent form obtained from all participants. The frequency of adults (>18 years) was 45 (73.8%) and children’s (<18 years) was 16 (26.2%); the frequency of male was 27 (44.3%) and 34 were female (55.7%). Hemoglobin estimation and red cell indices were carried out using the automatic blood cell counter Sysmex K × 21N. The results showed that Hb and RBCs indices were varied between mild to moderate and severe decreasing, hemoglobin concentration (Hb) with the mean value of 9.6 g/dL, with minimum value of 6.1 g/dl and maximum of 11.9 g/dl, while RBCs were increased in all patients, mean value 5.2 c/l, mean corpuscular volume mean was 58.9 fl, hematocrit was 30.4, mean corpuscular hemoglobin (MCH) 18.8 pg, mean corpuscular hemoglobin concentration (MCHC) was 31.7pg, and RDW was 18.8%. The method used for hemoglobin electrophoresis was capillary electrophoresis, Hb pattern shows increased HbA2 and HbF, the mean of HbA is 78.3%, HbF is 2.3%, and HbA2 is 6.5% with the min. value of 3.6% and max. of 12.2%. While the mean of serum iron was 82.75 μg/dl, 7 patients showed low level, 19 high level, and 35 were normal level. Comparison of hematological analysis (HbA2) in thalassemic patients coexisted with iron deficiency and without result was insignificant difference (p=0.645), this result disagrees with references that say iron deficiency masking HbA2. Nevertheless, the association between HbA2 and HbF revealed a statistically significant difference (p<0.013) and HbA2 with Hb was insignificant (p=0.260).

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