scholarly journals Fluorescence biomarkers of malignant melanoma detectable in urine

2020 ◽  
Vol 18 (1) ◽  
pp. 898-910
Author(s):  
Ivana Špaková ◽  
Katarína Dubayová ◽  
Vladimíra Nagyová ◽  
Mária Mareková
Keyword(s):  
Malignant Melanoma ◽  
Biological Material ◽  
Sampling Method ◽  
Histopathological Examination ◽  
Emission Spectra ◽  
Response To Therapy ◽  
Malignant Diseases ◽  
Medical Assistance ◽  
Suitable Alternative ◽  
Small Incidence

AbstractMalignant melanoma (MM) is a cancerous transformation of melanocytes. It is a disease with the worst response to therapy and, compared to other malignancies, presents much earlier with metastases. MM still belongs to relatively late-detected malignant diseases. Even so, the MM mortality rate is up to 96% for a relatively small incidence (5%). The gold standard for MM diagnosis is a histopathological examination that requires invasive surgery. An invasive sampling method of a biological material can be a stressful factor for the patient, which is often the reason why patients do not seek medical assistance as soon as possible. Our goal was to find a link between metabolites in urine and the stage of MM. Two excitation peaks at 360–370 nm and 450 nm were characterised in spectra of urine samples. The emission spectra have shown one significant peak at 410–460 nm. After addition of glutathione reductase to the samples, fluorescence dropped down only in patient samples and hidden fluorophores appeared. Malignant diseases are associated with the presence of specific metabolites that can be detected fluorescently in biological material such as urine, which can be a suitable alternative for an early detection of cancer or for tracking changes during and after treatment.

scholarly journals Nodular malignant melanoma in vulvar skin without pigmentation: a case report

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jing Nie ◽  
Yan Li ◽  
Xue Shen ◽  
Yan Liu ◽  
Haipeng Shi ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Histopathological Examination ◽  
Relevant Literature ◽  
Final Diagnosis ◽  
Interesting Case ◽  
Amelanotic Melanoma ◽  
Case Record ◽  
Diagnostic Pitfall ◽  
Asian Patients ◽  
Clinic Practice

Abstract Background Polypoid nodule growing without apparent pigmentation on the vulvar skin usually reminds us of the diagnostic pitfall, which is commonly and mistakenly diagnosed as other types of tumors. Although there are several manifestations of amelanotic melanoma are known, these malignancies are usually pigmented because they are derived from melanocytes containing melanin. However, amelanotic melanomas are easily misdiagnosed or their diagnoses were commonly delayed due to lack of pigmentation. Therefore, a solitary polypoid nodule is worth noting and further reporting. Particularly, the clinical characteristics and outcomes of the solitary polypoid nodule are rare in Asian patients. Case presentation We presented an interesting case of a 33-year-old female with a solitary polypoid nodule without apparent pigmentation on her vulvar skin. Her medical history was unclear, no ulcer was seen in the lesion area, and dermatoscopy was indicated a possible tumorous change, which has caught the attention of clinicians, and then further examined by the pathologist. The final diagnosis was nodular malignant melanoma (NM) (Breslow thickness 9.5mm, Clark level 4). Conclusions Hence, though reviewing this case record, the relevant literature and NM-related materials, we suggest that the combination of skin imaging technology and histopathological examination could provide us a better understanding and reduce the possibility of misdiagnosis in clinic practice.

Malignant melanoma in childhood: clinical course and response to chemotherapy.

1984 ◽  
Vol 2 (11) ◽  
pp. 1229-1234 ◽  
Author(s):  
F A Hayes ◽  
A A Green
Keyword(s):  
Malignant Melanoma ◽  
Metastatic Disease ◽  
Clinical Course ◽  
Objective Response ◽  
Response To Therapy ◽  
Adult Patients ◽  
Age Group ◽  
Number Of Children ◽  
Response To Chemotherapy

Malignant melanoma is uncommon in patients less than 20 years of age, and little is known about the response to chemotherapy in this age group. We report here a series of 18 children, of whom ten with metastatic disease were treated with cyclophosphamide, vincristine, and dactinomycin. Only two of nine evaluable patients failed to show an objective response to therapy. Five of ten patients treated are alive and free of tumor 12 to 80 months from the start of chemotherapy. Three have been in remission for more than five years. Results suggest that melanoma in this age group may be more responsive to chemotherapy than melanoma in adult patients and that a trial of this therapy in a larger number of children with evaluable disease is warranted.

scholarly journals Primary Spinal Cord Melanoma of Intradural Extramedullary Origin

2019 ◽  
Vol 10 (03) ◽  
pp. 522-525
Author(s):  
Ashish Sharma ◽  
Virendra Deo Sinha
Keyword(s):  
Spinal Cord ◽  
Malignant Melanoma ◽  
Histopathological Examination ◽  
Lower Limbs ◽  
Primary Malignant Melanoma ◽  
Magnetic Resonance Imaging Scan ◽  
Lumbosacral Region ◽  
Intensity Lesion ◽  
S 100 ◽  
L1 And L2

AbstractPrimary malignant melanoma is the very rare entity of the central nervous system. It accounts only 1% of all melanoma cases. A 67-year-old patient presented to us with lower back pain radiating to the left lower limb and progressive weakness of both lower limbs. She underwent magnetic resonance imaging scan of lumbosacral region that suggested T1 hyperintense, T2 hypointense, extramedullary altered signal intensity lesion at L1 and L2 vertebral level compressing terminal spinal cord, and cauda equine region. On histopathological examination, findings were suggestive of malignant melanoma that was confirmed with immunohistochemistry examination (positive for S-100, Melan-A, and HMB-45). Here, we are discussing an unusual case of primary malignant melanoma of conus and cauda equine region with comprehensive review regarding management and prognosis of the tumor.

scholarly journals Conservative management of primary malignant melanoma of the bladder: a case report

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Sebastiano Rapisarda ◽  
Maida Bada ◽  
Andrea Polara ◽  
Felice Crocetto ◽  
Massimiliano Creta ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Bladder Tumor ◽  
Histopathological Examination ◽  
Correct Diagnosis ◽  
Clinical History ◽  
Metastatic Malignant Melanoma ◽  
Primary Malignant Melanoma ◽  
Bacillus Calmette Guerin ◽  
Endoscopic Evaluation ◽  
Case Presentation

Abstract Background Primary malignant melanoma (PMM) of the bladder represents a very rare clinic-pathologic entity. Given the rarity of the disease, the best treatment option is not well recognized. Case presentation We describe a case of neoplasm of the bladder in a 74 years-old Caucasian man presenting with massive hematuria. Based on clinical, instrumental and histological findings a diagnosis of PMM was made. The patient underwent trans urethral resection of bladder tumor plus intravesical Bacillus Calmette–Guérin. Conclusions To make a correct diagnosis, clinical history, endoscopic evaluation, histopathological examination and immunohistochemistry, are necessary. Multidisciplinary evaluation is required to discriminate primary from metastatic malignant melanoma.

scholarly journals Primary Malignant Melanoma of the Tonsil : A Case Report

2009 ◽  
Vol 22 (2) ◽  
pp. 279-281
Author(s):  
SM Badruddoza ◽  
S Naz
Keyword(s):  
Case Report ◽  
Malignant Melanoma ◽  
Sore Throat ◽  
Male Patient ◽  
Clinical Examination ◽  
Histopathological Examination ◽  
Primary Malignant Melanoma ◽  
Polypoid Mass ◽  
Current Case ◽  
History Of

Malignant melanoma is a neoplasm of melanocytes. Primary malignant melanoma of the oronasal region is rare. When it does occur, it is difficult to manage, and the prognosis is not good. The current case was a 48 years old male patient who presented with history of sore throat, pain in left tonsillar region, hemoptysis with difficulty in swallowing. Clinical examination revealed a blackish ulcerated polypoid mass in the left palatine tonsil. Left sided tonsilectomy was done. Histopathological examination revealed primary malignant melanoma of the tonsil.TAJ 2009; 22(1): 279-281

scholarly journals A Clinical Characters and Prognosis Analysis on 15 Cases With Malignant Melanoma in the Head and Neck

2020 ◽  
Author(s):  
Xiaodong Liu ◽  
Weixian Liu ◽  
Qiuxu Wang
Keyword(s):  
Malignant Melanoma ◽  
Parotid Gland ◽  
Head And Neck ◽  
Histopathological Examination ◽  
Maxillofacial Surgery ◽  
Neck Region ◽  
Surgical Excision ◽  
Pathological Examination ◽  
Oral And Maxillofacial Surgery ◽  
Golden Standard

Abstract Objective: We analyze the pathogenesis, clinical characteristics, diagnosis, treatment and histological feature of Malignant Melanoma in the head and neck. To improve the understanding and help the early diagnosis and treatment of the disease. As a result, improve the treatment and prognosis of Malignant Melanoma in head and neck.Method: We collect 15 cases of Malignant Melanoma in the head and neck region treated in the Department of Oral and Maxillofacial Surgery in Shengjing Hospital of China Medical University. All data were obtained from patient’s medical records which including the pathogenesis, clinical and histological features, diagnosis, treatment and prognosis.Result: This study included 15 cases, there are 9 male and 6 female patients. The average age is 62.9 years lid, the oldest one is 75 years old and the youngest one is 40 years old. 2 cases occurred in the lip, 2 cases occurred in the tongue, 2 cases occurred in the submandibular area, 1 cases occurred in the parotid gland, 1 case occurred in the neck, other 7 cases occurred in the gingiva. The cases occurred in the lip, gingiva, tongue, parotid gland and submandibular area are primary tumor, cases occurred in neck were metastasis. 6 cases were treated by surgical excision. 1 cases occurred in gingiva was treated by surgical excision combine with chemotherapy. 5 years followed up has been taken. 10 cases were dead because of recurrence and metastasis in 5 years after the operation. 5 patients do not have a relapse and still alive.Conclusion: Malignant Melanoma in the head and neck are mostly affected the old people. In this study gingiva is the most commonly primary site, neck is the most commonly transferring site. The diagnosis of malignant Melanoma is mainly relying on the clinical manifestation and pathological examination. Histopathological examination is considered the golden standard for diagnosis. Surgical excision combined with chemotherapy is the main treatment modality for Malignant Melanoma. Cryotherapy and immunotherapy also have been used in the treatment. The prognosis of Malignant Melanoma is poor. So early detection and early treatment is important in the treatment.

scholarly journals Primary malignant melanoma of vagina: a case report

2020 ◽  
Vol 9 (8) ◽  
pp. 3499
Author(s):  
Smruti Shambharkar ◽  
Tarachand Sharma
Keyword(s):  
Malignant Melanoma ◽  
Firm Growth ◽  
Histopathological Examination ◽  
Lateral Wall ◽  
Primary Malignant Melanoma ◽  
Malignant Neoplasms ◽  
Vaginal Examination ◽  
Aggressive Disease ◽  
History Of ◽  
Worse Prognosis

Primary malignant melanoma of the vagina is a rare and aggressive disease with worse prognosis as compared with non-genital melanomas or other vaginal malignant neoplasms. Presented here is a case of 42 years female with 3 months history of amenorrhea and vaginal discharge. On vaginal examination, a firm growth of size approximately 7-8 cm was found attached to the left postero-lateral wall of vagina and extending up to the introitus. On biopsy and histopathological examination, it was diagnosed as a case of high-grade malignant melanoma of amelanotic type. Radiotherapy was started as a part of treatment after consultation with an oncologist, considering non-resectable nature of the mass. The patient received first 5 cycles of radiotherapy but succumbed to the disease during treatment.

scholarly journals Autofluorescence of atmospheric bioaerosols – fluorescent biomolecules and potential interferences

2011 ◽  
Vol 4 (5) ◽  
pp. 5857-5933 ◽  
Author(s):  
C. Pöhlker ◽  
J. A. Huffman ◽  
U. Pöschl
Keyword(s):  
Biological Material ◽  
Aerosol Particles ◽  
Measurement Data ◽  
Emission Spectra ◽  
Biological Materials ◽  
Substantial Contribution ◽  
Light Sources ◽  
Atmospheric Measurements ◽  
Biological Origin ◽  
Biological Aerosol Particles

Abstract. Primary biological aerosol particles (PBAP) are an important subset of air particulate matter with a substantial contribution to the organic aerosol fraction and potentially strong effects on public health and climate. Recent progress has been made in PBAP quantification by utilizing real-time bioaerosol detectors based on the principle that specific organic molecules of biological origin such as proteins, coenzymes, cell wall compounds and pigments exhibit intrinsic fluorescence. The properties of many fluorophores have been well documented, but it is unclear which are most relevant for detection of atmospheric PBAP. The present study provides a systematic synthesis of literature data on potentially relevant biological fluorophores. We analyze and discuss their relative importance for the detection of fluorescent biological aerosol particles (FBAP) by online instrumentation for atmospheric measurements such as the ultraviolet aerodynamic particle sizer (UV-APS) or the wide issue bioaerosol sensor (WIBS). In addition, we provide new laboratory measurement data for selected compounds using bench-top fluorescence spectroscopy. Relevant biological materials were chosen for comparison with existing literature data and to fill in gaps of understanding. The excitation-emission matrices (EEM) exhibit pronounced peaks at excitation wavelengths of ~280 nm and ~360 nm, confirming the suitability of light sources used for online detection of FBAP. They also show, however, that valuable information is missed by instruments that do not record full emission spectra at multiple wavelengths of excitation, and co-occurrence of multiple fluorophores within a detected sample will likely confound detailed molecular analysis. Selected non-biological materials were also analyzed to assess their possible influence on FBAP detection and generally exhibit only low levels of background-corrected fluorescent emission. This study strengthens the hypothesis that ambient supermicron particle fluorescence in wavelength ranges used for most FBAP instruments is likely to be dominated by biological material and that such instrumentation is able to discriminate between FBAP and non-biological material in many situations. More detailed follow-up studies on single particle fluorescence are still required to reduce these uncertainties further, however.

scholarly journals Confluent and Reticulated Papillomatosis of Carteaud and Gougerot in a Young Nepali Male

2020 ◽  
Vol 18 (1) ◽  
pp. 64-66
Author(s):  
Bibush Amatya ◽  
Rashmi Sharma ◽  
MM Aarif Syed ◽  
Aasiya Rajbhandari
Keyword(s):  
Complete Resolution ◽  
Histopathological Examination ◽  
Clinical Findings ◽  
Pityriasis Versicolor ◽  
Response To Therapy ◽  
First Line ◽  
Topical Tretinoin ◽  
First Case ◽  
Gradual Onset ◽  
Histopathologic Changes

Confluent and reticulated papillomatosis of Carteaud and Gougerot is a keratinization disorder with an infective aetiology. Patients present with hyperpigmented papules on the upper trunk and axillae that coalesce centrally and demonstrate reticulation peripherally. Diagnosis is based on clinical findings, characteristic histopathologic changes and response to therapy. We report a case of a young Nepali male who presented with gradual onset of asymptomatic raised dark brown lesions on his neck, trunk and axillae over the course of eight years. The condition was previously misdiagnosed as pityriasis versicolor and had received oral and topical antifungals. The diagnosis was revised to confluent and reticulated papillomatosis based on clinical and histopathological examination. He was subsequently started on oral minocycline 50 mg twice daily and nightly application of topical tretinoin 0.05% gel. There was complete resolution of all his lesions except for residual hyperpigmentation at the end of two months of therapy. There has been no relapse six months from the end of therapy.  This is to our knowledge, the first case of confluent and reticulated papillomatosis reported from Nepal. Oral minocycline and topical tretinoin should be considered first line in the treatment of confluent and reticulated papillomatosis.

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