Comparison of different autoanalyzers for the determination of lymphocyte and neutrophil counts in mouse blood

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Saurabh Kumar Gupta ◽  
Dievya Gohil ◽  
Girish Ch. Panigrahi ◽  
Swati Vaykar ◽  
Pallavi Rane ◽  
...  
Keyword(s):  
Neutrophil Count ◽  
Gold Standard ◽  
Limits Of Agreement ◽  
Mouse Blood ◽  
Blood Samples ◽  
Manual Counting ◽  
Bland Altman Method ◽  
High Level ◽  
Level Of Agreement

Abstract Objectives Autoanalyzers are used in clinical haematology for analysis of blood samples in clinical as well as in nonclinical studies. The results from these analyzers vary from machine to machine. In this study, we compared the lymphocyte and neutrophil count of mouse blood between ADVIA 2120i, Horiba Yumizen H2500 and CellaVision analyzers against manual counting as gold standard. Methods Blood samples from 28 female BALB/c mice were collected and analyzed. Agreement between different autoanalyzers and manual counting were determined by Bland–Altman method. Results A high level of agreement was found between CellaVision and manual technique for lymphocyte (Bias=4.75, 95% limits of agreement −14 to 24) and neutrophil count (Bias=0.68 (−17 to 19)). Agreement in lymphocyte count was also observed between ADVIA and manual counting, but to a lesser extent compared to CellaVision (Bias=13.9 (−10.45 to 38.27)). However, no agreement was observed for ADVIA (Neutrophils), Horiba (lymphocytes and neutrophils) with manual counting. Conclusions Our data suggests that CellaVision could be used for the differential counting of neutrophil and lymphocytes in mouse blood sample.

scholarly journals Analysis of Agreement between Expired-Air Carbon Monoxide Monitors

2016 ◽  
Vol 12 (2) ◽  
pp. 105-112 ◽  
Author(s):  
Joshua L. Karelitz ◽  
Valerie C. Michael ◽  
Kenneth A. Perkins
Keyword(s):  
Carbon Monoxide ◽  
Paired Data ◽  
Limits Of Agreement ◽  
Bland Altman Method ◽  
Dsm Iv ◽  
Cigarette Dependence ◽  
Mean Differences ◽  
Level Of Agreement ◽  
Expired Air

Introduction: The current study examined the level of agreement in expired-air carbon monoxide (CO) values, focusing especially on those confirming abstinence, between the two most commonly used CO monitors, the Vitalograph BreathCO and the Bedfont piCO+ Smokerlyzer.Methods: Expired-air samples were collected via both monitors from adult dependent smokers (44 M, 34 F) participating in studies using CO values to confirm abstinence durations of: 24 hours, 12 hours, or no abstinence. All met DSM-IV nicotine dependence criteria and had a mean (SD) Fagerström Test of Cigarette Dependence score of 5.1 (1.8). Paired data collected across multiple visits were analyzed by regression-based Bland–Altman method of Limits of Agreement (LoA).Findings: Analysis indicated a lack of agreement in CO measurement between monitors. Overall, the Bedfont monitor gave mean (±SEM) readings 3.83 (±0.23) ppm higher than the Vitalograph monitor. Mean differences between monitors were larger for those ad lib smoking (5.65 ± 0.38 ppm) than those abstaining 12–24 hours (1.71 ± 0.13 ppm). Yet, there also was not consistent agreement in classification of 24-hour abstinence between monitors.Conclusions: Systematic differences in CO readings demonstrate these two very common monitors may not result in interchangeable values, and reported outcomes in smoking research based on CO values may depend on the monitor used.

scholarly journals Comparison of Two Derivatization Methods for the Analysis of Fatty Acids andTransFatty Acids in Bakery Products Using Gas Chromatography

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Jumat Salimon ◽  
Talal A. Omar ◽  
Nadia Salih
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Methyl Esters ◽  
Correlation Coefficients ◽  
Bakery Products ◽  
Trimethylsilyl Diazomethane ◽  
Acid Catalyzed ◽  
High Level ◽  
Level Of Agreement

Two different procedures for the methylation of fatty acids (FAs) andtransfatty acids (TFAs) in food fats were compared using gas chromatography (GC-FID). The base-catalyzed followed by an acid-catalyzed method (KOCH3/HCl) and the base-catalyzed followed by (trimethylsilyl)diazomethane (TMS–DM) method were used to prepare FA methyl esters (FAMEs) from lipids extracted from food products. In general, both methods were suitable for the determination ofcis/transFAs. The correlation coefficients (r) between the methods were relatively small (ranging from 0.86 to 0.99) and had a high level of agreement for the most abundant FAs. The significant differences (P=0.05) can be observed for unsaturated FAs (UFAs), specifically for TFAs. The results from the KOCH3/HCl method showed the lowest recovery values (%R) and higher variation (from 84% to 112%), especially for UFAs. The TMS-DM method had higherRvalues, less variation (from 90% to 106%), and more balance between variation and %RSD values in intraday and interday measurements (less than 4% and 6%, resp.) than the KOCH3/HCl method, except for C12:0, C14:0, and C18:0. Nevertheless, the KOCH3/HCl method required shorter time and was less expensive than the TMS-DM method which is more convenient for an accurate and thorough analysis of richcis/transUFA samples.

Absolute Circulating Pericyte Progenitor Cell Counts in Mice by Flow Cytometry: comparison of 2 single-platform technologies

2015 ◽  
Vol 30 (4) ◽  
pp. 434-438 ◽  
Author(s):  
Xiaochen Yuan ◽  
Qingbin Wu ◽  
Hongwei Li ◽  
Bingwei Li ◽  
Ruijuan Xiu
Keyword(s):  
Linear Correlation ◽  
Interobserver Agreement ◽  
Intraclass Correlation ◽  
Blood Samples ◽  
Cell Counts ◽  
Angiogenic Biomarkers ◽  
The Stability ◽  
High Level ◽  
Level Of Agreement

Background Accumulating evidence indicates that circulating pericyte progenitor cells (CPPCs) may be angiogenic biomarkers in cancer and diabetes. Their validity as biomarkers depends on the accuracy of techniques used for enumeration. In this report, absolute CPPC counts were performed by 2 single-platform technologies. The reliability of the 2 methods, including retest reliability and intraobserver and interobserver variability, was assessed according to the intraclass correlation coefficient (ICC). The linear correlation and agreement among both methods were assessed, and the stability of CPPC numbers in blood samples was analyzed. Methods The blood samples were obtained from ICR mice. The samples were processed through a no-lyse, 1-wash procedure, and Syto16+CD45-CD31-CD140b+ CPPCs were analyzed by exclusion of dead cells and by fluorescence-minus-one control. CPPCs were enumerated by 2 methods: bead-based 123count eBeads count (eBioscience) and direct volume–based Accuri C6 Flow Cytometer count (BD). The cells were measured immediately and after storage of blood samples for 24 and 48 hours. Results There were excellent retest correlations and intraobserver and interobserver agreement in both methods. The 2 methods showed a high linear correlation (R2 = 0.923) and with a high level of agreement (0.986). It was demonstrated that CPPCs are unstable in blood samples. Conclusions In this study, 2 reproducible protocols for CPPC quantification were established. These protocols should facilitate future studies to further define the role of CPPCs as cellular biomarkers.

scholarly journals Comparison of phoropter and trial frame-based von Graefe heterophoria measures in non-presbyopic participants

2021 ◽  
Vol 80 (1) ◽  
Author(s):  
Annah L. Tsotetsi ◽  
Solani D. Mathebula
Keyword(s):  
South Africa ◽  
University Students ◽  
Limits Of Agreement ◽  
Near Vision ◽  
Subjective Measurement ◽  
Normal University ◽  
Standard Deviations ◽  
High Level ◽  
Level Of Agreement

Background: There are several clinical techniques for the subjective measurement of heterophoria. In South Africa, von Graefe (VG) is a commonly used technique to quantify heterophoria using the phoropter.Purpose: The purpose of the study was to investigate the agreement of VG heterophoria measurement using the phoropter and a trial frame.Setting: The study was conducted at an Optometry Clinic, University of Limpopo, South Africa.Methods: Both distance and near horizontal and vertical heterophoria VG measurements were performed on 88 visually-normal university students using the phoropter and trial frame. The handheld rotary prism was used on the trial frame. The 95% limits of agreement in prism diopters were compared using the Bland-Altman statistical test.Results: For distance horizontal heterophoria, the VG means were 0.39 exophoria ± 2.0Δ and 0.38 exophoria ± 1.8Δ with the phoropter and trial frame respectively. The means for near horizontal heterophoria were 3.69 exophoria ± 3.3Δ and 4.13 exophoria ± 3.27Δ with the phoropter and trial frame. For the vertical heterophoria at distance, the means and standard deviations were 0.18 hyperphoria ± 0.74 and 0.13 hyperphoria ± 0.07Δ, while at near vision they were 0.03 hyperphoria ± 0.71 and 0.07 hyperphoria ± 0.71Δ, respectively.Conclusion: Measurement of VG heterophoria testing using the phoropter and trial frame showed a high level of agreement for both distance and near vision performed through the phoropter and a trial frame. For clinical and research purposes, the phoropter and trial frame can be used interchangeably for measuring heterophoria.

Simple Graphical Method for Detection of a Partial Blockage Or Leak in a Single Pipeline

2021 ◽  
Author(s):  
Prabhat Kumar ◽  
Pranab Mohapatra
Keyword(s):  
Uncertainty Analysis ◽  
Frequency Domain ◽  
Graphical Method ◽  
Low Frequencies ◽  
Frequency Responses ◽  
Frequency Domain Methods ◽  
Fault Parameters ◽  
High Level ◽  
Level Of Agreement

Abstract Transient-based methods in the frequency domain are used for fault detection in pipes. However, the required measurements are performed with a large number of valve frequencies. Sometimes the number of frequencies in traditional frequency-domain methods can be in the hundreds. More runs are required with higher harmonics when the required number of frequencies is more. The current study aims to overcome this difficulty of requirement of higher number of frequencies. The location and the size of a single leak or a single discrete blockage are proposed to be predicted using two appropriately chosen low frequencies. Iso-Pressure Frequency Responses (IPFRs) are generated by performing numerical experiments with these two low frequencies enabling determination of the fault. The methodology is demonstrated through various numerical examples. The results show that the procedure is quite accurate, with a high level of agreement between the actual and the predicted fault parameters. The error in the result is of the order of the chosen discretization. An uncertainty analysis is performed to illustrate that the prediction error caused by the error in the measurements of the pressure frequency responses (PFRs) depends on the location and the size of the fault itself. The error in prediction results is analyzed in uncertainty analysis with 0.10%, 0.15%, and 0.20% error in the peak head measurement. For the same error in the PFR measurement, the fault lying in a thin density of the contours would lead to a higher error in the final solution.

scholarly journals Creatinine clearance after cimetidine administration in a new short procedure: comparison with plasma and renal clearances of iohexol

2019 ◽  
Vol 13 (4) ◽  
pp. 587-596 ◽  
Author(s):  
Thomas Stehlé ◽  
Khalil El Karoui ◽  
Mehdi Sakka ◽  
Ahmad Ismail ◽  
Marie Matignon ◽  
...  
Keyword(s):  
Creatinine Clearance ◽  
Good Precision ◽  
Limits Of Agreement ◽  
Reference Limits ◽  
Before And After ◽  
Renal Clearances ◽  
The Mean ◽  
High Level ◽  
Level Of Agreement ◽  
Oral Cimetidine

Abstract Background Creatinine clearance after cimetidine administration (Cim-CreatClr) was once proposed as a method of glomerular filtration rate (GFR) measurement, but has been largely abandoned. We investigated whether a new short procedure for Cim-CreatClr determination could be considered an appropriate method for GFR measurement. Methods A 150-min protocol involving oral cimetidine administration was developed to determine Cim-CreatClr. In total, 168 patients underwent simultaneous assessments of creatinine clearance before and after cimetidine administration [basal creatinine clearance (Basal-CreatClr) and Cim-CreatClr, respectively], renal iohexol clearance and plasma iohexol clearance (R-iohexClr and P-iohexClr, respectively). We compared the agreement between the various methods of GFR measurement, using Bland–Altman plots to determine biases, precisions (standard deviation of the biases) and accuracy (proportions of GFR values falling within 10, 15 and 30% of the mean: P10, P15 and P30, respectively). Results After cimetidine administration, Basal-CreatClr decreased by 19.8% [95% reference limits of agreement (95% LoA): −2.2 to 41.7%]. The bias between Cim-CreatClr and P-iohexClr was −0.6% (95% LoA −26.8 to 28%); the precision was 14.0%; P10, P15 and P30 were 57.1% [95% confidence interval (95% CI) 49.3 to 64.7%], 73.2% (95% CI 65.8 to 79.7%) and 97.0% (95% CI 93.2 to 99.0%), respectively. Due to the positive bias (16.7%; 95% LoA −3.6 to 36.9%) of Cim-CreatClr relative to R-iohexClr, accuracy of Cim-CreatClr relative to R-iohexClr was poor despite a good precision (10.3%). Conclusions Our study shows a high level of agreement between Cim-CreatClr and P-iohexClr. These results suggest that this short Cim-CreatClr procedure is a valid method for GFR measurement, which might be useful, in particular, in situations in which P-iohexClr is not suitable or not available.

scholarly journals COMPARATIVE ANALYSIS OF DIAGNOSTIC KITS FOR THE QUANTITATIVE DETERMINATION OF HIV-1 RNA PRODUCED IN RUSSIA

2020 ◽  
Vol 12 (1) ◽  
pp. 123-133
Author(s):  
I. A. Lapovok ◽  
A. E. Lopatukhin ◽  
A. A. Kirichenko ◽  
A. V. Murzakova ◽  
D. E. Kireev
Keyword(s):  
Comparative Analysis ◽  
Clinical Samples ◽  
Plasma Samples ◽  
Spearman Correlation ◽  
Maximum Difference ◽  
Diagnostic Kits ◽  
Bland Altman Method ◽  
High Level ◽  
Hiv 1

Aim: to carry out the comparative analysis of Russian kits for HIV-1 viral load (VL) measuring: AmpliSens HIV-monitor-FRT, AmpliSens HIV-monitor-Duo-FL, RealBest RNA HIV quantitative, HIV-GEN quantitative.Materials and methods: we analyzed the collection of clinical blood plasma samples with VL 590–2,05×106 copies/ml, dilutions of HIV-1 2nd International Standard, as well as panels of subtypes and recombinant forms of HIV-1 group M. The convergence of the results was determined using the Spearman correlation analysis and the Bland-Altman method.Results. A high level of convergence of VL measuring results in clinical samples (n=76) was determined for all compared kits with the value of approximation reliability R of at least 0,85. The maximum difference between the median values of VL results (0,48 log, ≈3-fold) was obtained for HIVMonitor-FRT and RealBest quantitative, which did not exceed the acceptable discordance rate in two kits comparing. All dilutions of the 2nd international HIV-1 standard were successfully determined by all kits with middle error 0,16–0,34 lg copies/ml. All kits successfully revealed the most of HIV-1 subtypes and recombinants studied with middle deviation 0,02–0,56 log copies/ml. HIV-GEN quantitative demonstrated the highest accuracy, but didn’t reveal ADG-recombinant. 

The Comparability of Job Evaluation Methods in Supplying Approximately Similar Classifications in Rating One Job Series

1982 ◽  
Vol 12 (2) ◽  
pp. 38-40 ◽  
Author(s):  
R.J. Snelgar
Keyword(s):  
South Africa ◽  
Evaluation Method ◽  
Correlation Coefficients ◽  
Evaluation Methods ◽  
Job Evaluation ◽  
High Level ◽  
High Degree ◽  
Level Of Agreement ◽  
Similar Classification

There is a belief that any job evaluation method, when correctly applied to a series of jobs, will result in approximately the same rating classification as that supplied by any other method for the same job series. This study was aimed primarily at a determination of the extent to which a number of job evaluation methods, differing in methodology, and presently in use within South Africa on a national basis, would in fact supply similar classification. Correlation coefficients among the ratings supplied by participating organizations, in terms of points allocation for each job subsequent to evaluation according to job evaluation factors (raw point ratings), were unexpectedly high. These correlations ranged from 0,93 to 0,99, with an average of 0,98, indicating a remarkably high level of agreement. Correlations were properly transformed, and mean correlations of each organization with every other organization subsequently calculated. These mean correlations ranged from 0,96 to 0,98, once again indicating a high degree of commonality.

Immediate implantation in a patient with chronic generalized parodontitis and apical granuloma

2019 ◽  
Vol 24 (2) ◽  
pp. 145-149
Author(s):  
A. I. Musienko ◽  
K. I. Nesterova
Keyword(s):  
Tooth Extraction ◽  
Tooth Root ◽  
Duration Of Treatment ◽  
Promising Direction ◽  
Aesthetic Result ◽  
Bone Atrophy ◽  
Alveolar Tissue ◽  
High Level ◽  
Immediate Implantation

Relevance. Rehabilitation of patients with moderate to severe generalized periodontitis is a leading problem in periodontology. It was the determination of the prospects for immediate implantation in patients with chronic periodontitis, combined with the pathology of the tooth root and maxillary sinus.Materials and methods. A group of 94 people with periodontitis and chronic odontogenic rhinosinus was observed who underwent sinus surgical treatment, tooth extraction and one-stage implantation with FRP growth factor according to the author's technology.Results. The method showed high efciency on the basis of assessing the clinical, aesthetic result and restoration of bone density after surgery.Conclusions. The developed technology is a promising direction, it allows to combine a high level of sanation of alveolar tissue with the advantages of immediate implantation, prevents bone atrophy, helps reduce the duration of treatment and the number of surgical and orthopedic interventions.

Methodical approaches to managing economic safety of tourism enterprises

2020 ◽  
pp. 160-164
Author(s):  
Leonid Tsubov ◽  
Oresta Shcherban
Keyword(s):  
Safety Management ◽  
Integrated Approach ◽  
Small Enterprise ◽  
Complex Nature ◽  
Efficient Management ◽  
Tourism Enterprises ◽  
High Level ◽  
Functional Components ◽  
Stimulation Control

The set of scientific-methodological tools to secure the mechanism of economic safety management of tourism entrepreneurship is examined as an aggregate of methods, tools, and conceptual activities directed at maintaining the high level of economic safety of tourism entrepreneurship. The features of managing the tourism enterprise and economic safety are analyzed. The basic valuation principles of the reliability and efficiency of the economic safety of the tourism enterprise are determined. The basic tasks of ensuring the economic safety of a small enterprise are outlined. The need to use the integrated approach that secures more opportunities to avoid threats and limits the danger of their emergence is emphasized. The most important principles for securing the economic safety of the tourism enterprise on the microeconomic level are described. Possible practical methods of risk management for the implementation of adopted decisions are proposed. The paper proves the fact that the complex nature of the management of the economic safety of the tourism enterprise and securing the sufficiently efficient management system of detecting and eliminating the threats are provided by the establishment of the management of the economic safety system of the tourism enterprise and its functional components. Research of the methodical approaches to the management of the tourism enterprises’ economic safety allows building and describing the functional structure of the mechanism of management of the tourist enterprise’s economic safety (it is formalized and described by 5 functions: determination of aims; planning; organization and adjusting; motivation and stimulation; control and monitoring).

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