Chemical Composition and Antiproliferative and Antioxidant Activities of Methanolic Extract of Alcea setosa A. Malvaceae

Alcea setosa A. (Malvaceae) is a wild plant that grows in Jordan and have several traditional medicinal uses. This study aims to collect and chemically analyze the methanolic extract from Alcea setosa A. from Jordan and to evaluate its cytotoxic and antioxidant activity against human breast cancer cells (T47D), colorectal adenocarcinoma cells (CACO2), and normal human fibroblasts (MRC5). The extract was extracted by methanol solvent and analyzed by liquid chromatography coupled with a mass spectrometer. Cell viability was assessed using trypan blue, neutral red, and MTT assays, and antioxidant activity was evaluated using DPPH scavenging activity assay. A total of 290 compounds, 12 among which were identified when compared to available standards, the extract contained six flavons derivatives, Two fatty acids, one ketone derivative, one flavonol derivative, one organic acid, and one coumarin derivative. The results also revealed that the IC50 values of the viability assays were higher among normal cells compared to the human cancer cell lines, and the viability inhibition was significant at higher concentrations compared to untreated cells. Nevertheless, moderate antioxidant activity was observed for the extract in the DPPH scavenging activity test. To sum up, this study indicates that samples of A. setosa collected from Jordan is likely to be an effective antioxidant, is optimistically potential to be utilized in breast and colon cancers treatment due to its preferential cytotoxicity against cancer cells.

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Ganoderma lucidum Metanolik Ekstraktının Fitotoksik Etkilerinin ve Antioksidan Potansiyelinin Araştırılması

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v4i3.163-170.568 ◽

2016 ◽

Vol 4 (3) ◽

pp. 163 ◽

Cited By ~ 1

Author(s):

Fuat Bozok ◽

Tülin Eker ◽

Gökhan Sezer ◽

Adnan Bozdoğan ◽

Hasan Hüseyin Doğan ◽

...

Keyword(s):

Nitric Oxide ◽

Ganoderma Lucidum ◽

Methanolic Extract ◽

Antioxidant Activities ◽

Reducing Power ◽

Scavenging Activity ◽

Phytotoxic Effect ◽

Dpph Scavenging Activity ◽

Allelopathic Effects ◽

Dpph Scavenging

The aim of this study is to reveal allelopathic effects and antioxidant activities of methanolic extract of Ganoderma lucidum in Zorkun plateau (Osmaniye). It was determined that methanolic extract of G. lucidum has significantly phytotoxic effect by increasing the doses (1, 2, 4, 8 mg/mL) on Hordeum vulgare and Triticum aestivum. Total phenol and flavonoid amounts of G. lucidum were 114.55 mg/kg and 8.95 mg/kg, respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), scavenging activity (60%, 91% and 92%), reducing power (0.83, 1.43 and 2.23 Abs), and nitric oxide (NO) scavenging activity (48%, 55% and 70%) of this mushroom at the different concentrations (1.25, 2.5 and 5 mg/mL) were determined, respectively. However, H2O2 scavenging activity of the different concentrations (0.0156-0.125 mg/mL) was found as 18%, 56%, 60% and 86%, respectively. Based on these findings, it can suggest that G. lucidum methanolic extract has significantly phytotoxic effect and antioxidant activity. The present study is the first report on the phytotoxic effects and antioxidant activities of G. lucidum which consumed and collected from Amanos Mountains.

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Presumptive Relationship between Oxidoreduction Potential and Both Antibacterial and Antioxidant Activities of Herbs and Spices: Oxidoreduction Potential as a Companion Tool for Measuring the Antioxidant Activity

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47111301 ◽

2018 ◽

Vol 47 (2) ◽

pp. 506-514 ◽

Cited By ~ 4

Author(s):

Duried ALWAZEER ◽

Sally DHAM

Keyword(s):

Antioxidant Activity ◽

Plant Extracts ◽

Phenolic Content ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Scavenging Activity ◽

Dpph Scavenging Activity ◽

Dpph Scavenging ◽

Oxidoreduction Potential ◽

Herbs And Spices

Antibacterial and antioxidant activities form an essential component of the bioactive properties of plant products. The antibacterial and the antioxidant properties of three types of aqueous extracts of four herbs and spices were evaluated. While the total phenolic content, DPPH radical scavenging activity and oxidoreduction potential methods were used for the antioxidant properties determination, the inhibition diameters of extracts toward the growth of Staphylococcus warneri, Bacillus cereus, Enterobacter cloacae and Proteus vulgaris were evaluated. Although the phenolic content did not correlate with the antibacterial activity of plant extracts, it linearly correlated (R2 = 0.827) with DPPH scavenging activity of the studied plants following the order: sumac > ginger > rosemary > cinnamon. The oxidoreduction potential values linearly correlated with both the phenolic content (R2 ≈ 0.88) and DPPH scavenging activity values (R2 ≈ 0.96). The oxidoreduction potential could be proposed as a useful companion tool combined with other techniques when determining the antioxidant activity of plant extracts and food products is considered.

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Antioxidant activity of crude ethanolic extract and fractions of Ziziphus mauritiana Lam. (Rhamnaceae) leaves from Burkina Faso

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2017-0176 ◽

2019 ◽

Vol 30 (3) ◽

Author(s):

Estelle N.H. Youl ◽

Cyrille A.P. Ouédraogo ◽

Moustapha Gambo ◽

Moussa Ouédraogo ◽

Martin Kiendrebéogo ◽

...

Keyword(s):

Antioxidant Activity ◽

Burkina Faso ◽

Antioxidant Activities ◽

Ethanolic Extract ◽

Ziziphus Mauritiana ◽

Scavenging Activity ◽

Antioxidant Power ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

Abstract Background Ziziphus mauritiana Lam. is a plant used in traditional medicine in Burkina Faso in the treatment of several diseases, of which diabetes is characterized by oxidative stress. The aim of this study was to evaluate the in vitro antioxidant potential of the extracts of leaves of this plant. Methods The crude hydroethanolic extract (HEE) of the leaves of Z. mauritiana and their partitionates in n-hexane, dichloromethane, and ethyl acetate, and in the residual aqueous solution (the F1, F2, F3, and F4 fractions, respectively) were first prepared. The content of polyphenols was determined and the antioxidant effects of the extracts were evaluated by their 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, inhibition of lipid peroxidation (TBARS), and the ferric reducing antioxidant power (FRAP). Results The HEE as well as the F3 and F4 fractions were rich in polyphenols with contents between 58 and 84 mg equivalent gallic acid per 100 mg. The flavonoid content was 4 mg quercetin equivalents in the HEE and the F4 fraction. Except for the F1 fraction, the HEE and the other fractions showed significant DPPH scavenging activity (IC50 between 8 and 12 μg/mL). The IC50 of TBARS by different extracts was in the range 1–5 μg/mL, and the FRAP activity was 7–85 mg ascorbic acid equivalent per 100 mg. Total polyphenol content was highly correlated with the antioxidant activities. Conclusions The HEE, F3, and F4 fractions were found to be the richest in polyphenols and had the best antioxidant activity. The antioxidant activity of the extracts of the leaves of Z. mauritiana is due to these polyphenolic compounds.

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Stem Lettuce and Its Metabolites: Does the Variety Make Any Difference?

Foods ◽

10.3390/foods10010059 ◽

2020 ◽

Vol 10 (1) ◽

pp. 59

Author(s):

Janusz Malarz ◽

Klaudia Michalska ◽

Anna Stojakowska

Keyword(s):

Chemical Composition ◽

Plant Material ◽

Methanolic Extract ◽

Sesquiterpene Lactones ◽

Scavenging Activity ◽

Nmr Analysis ◽

Caffeic Acid Derivatives ◽

Aerial Parts ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

The objective of the present study was to characterize chemical composition of hitherto unexamined aerial parts of Lactuca sativa var. angustana cv. Grüner Stern. In contrast to leafy and head varieties of the lettuces, asparagus lettuce grown in Europe is much less studied. Fractionation of a methanolic extract from leaves of L. sativa cv. Grüner Stern, supported with HPLC/DAD and 1H NMR analysis, led to the isolation and/or identification of numerous terpenoid and phenolic compounds, including five apocarotenoids—(-)-loliolide, (+)-dehydrovomifoliol, blumenol A, (6S,9S)-vomifoliol, and corchoionoside C; three sesquiterpene lactones; two lignans—((+)-syringaresinol and its 4-O-β-glucoside); five caffeic acid derivatives; and three flavonoids. Some of the compounds, to the best of our knowledge, have never been isolated from L. sativa before. Moreover, monolignols, phenolic acids and a tryptophan-derived alkaloid were found in the analyzed plant material. Stems, leaves and shoot tips of the asparagus lettuce were examined to assess their phenolics and sesquiterpene lactone content as well as DPPH scavenging activity. Another stem lettuce—L. sativa var. angustana cv. Karola, two cultivars of leafy lettuces and one species of wild lettuce—L. serriola, were also examined as a reference material using HPLC/DAD. The results have been discussed regarding our previous studies and the literature data available.

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Sunflower (Helianthus annuus L.) Plants at Various Growth Stages Subjected to Extraction—Comparison of the Antioxidant Activity and Phenolic Profile

Antioxidants ◽

10.3390/antiox9060535 ◽

2020 ◽

Vol 9 (6) ◽

pp. 535 ◽

Cited By ~ 1

Author(s):

Francesco Gai ◽

Magdalena Karamać ◽

Michał A. Janiak ◽

Ryszard Amarowicz ◽

Pier Giorgio Peiretti

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Total Phenolic ◽

Growth Stages ◽

Scavenging Activity ◽

Flowering Stage ◽

Phenolic Profile ◽

Aerial Parts ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

The aim of this study was to evaluate the differences in the antioxidant activity and phenolic profile of sunflower (Helianthus annuus L.) extracts obtained from the aerial parts of plants harvested at five growth stages. In vitro assays were used to determine the antioxidant activity, i.e., ABTS•+ and DPPH• scavenging activity, the ferric-reducing antioxidant power (FRAP) and the ability to inhibit β-carotene–linoleic acid emulsion oxidation. Phenolic compounds, such as mono- and dicaffeoylquinic acid isomers and caffeic acid hexose, were identified using the LC–TOF–MS/MS technique. The predominant compound during the growth cycle of the plant was 3,5-di-O-caffeoylquinic acid, whose content was the highest at the mid-flowering stage. The total phenolic content was also the highest in sunflowers at the mid-flowering stage. The main phenolic compound contents were closely correlated with ABTS•+ and DPPH• scavenging activity and FRAP. No significant correlation was found between the total phenolic content and the antioxidant activity in the emulsion system. The highest antiradical activity and FRAP were generally determined in older plants (mid-flowering and late flowering stages). In conclusion, the aerial parts of sunflowers, in particular those harvested at the mid-flowering stage, are a good plant material from which to obtain phenolic compound extracts, albeit mainly of one class (esters of caffeic acid and quinic acid), with high antioxidant activity.

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PHYTOCHEMICAL IDENTIFICATION AND ANTIOXIDANT ACTIVITY OF PASSIFLORA FOETIDA FRUITS AND LEAVES EXTRACTS: A COMPARATIVE STUDY

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2020v12i6.31505 ◽

2020 ◽

pp. 55-58 ◽

Cited By ~ 1

Author(s):

YOHANES TANDORO ◽

PAINI SRI WIDYAWATI ◽

TARSISIUS DWI WIBAWA BUDIANTA ◽

GRACE SUMARGO

Keyword(s):

Antioxidant Activity ◽

Cardiac Glycosides ◽

Reducing Power ◽

Total Phenol ◽

Total Flavonoids ◽

Scavenging Activity ◽

Phytochemical Compounds ◽

Dpph Scavenging Activity ◽

Passiflora Foetida ◽

Dpph Scavenging

Objective: The objective of this study was to compare the phytochemical composition and antioxidant activity of Passiflora foetida fruits and leaves extract. Methods: The parameters observed in this study were phytochemical compounds including alkaloid, flavonoid, phenolic, sterol, triterpenoid, saponin, tannin, and cardiac glycoside, total phenolic content Folin Ciocalteu method is based on reduction of Folin Ciocalteu reagent in alkaline medium; the metal complex produced measured at λmax: 760 nm; total flavonoids content with AlCl3 Colorimetric method based on complex formation of AlCl3 and flavonoid content in alkaline medium, the AlCl3-flavonoid complex produced measured at λmax: 510 nm; free radical DPPH scavenging activity; and ferric reducing power based on reduction of Fe3+ion into Fe2+ion that reacted with FeCl3 to form a ferric-ferrous complex that measured at λmax: 700 nm. Results: Passiflora leaves extract has phytochemical compound such as alkaloids, phenolics, flavonoids, saponins, and cardiac glycosides, total phenol was 22.92±0.18 mg GAE/g sample dry base, total flavonoid was 7.01±0.10 mg CE/g sample dry base, DPPH scavenging activity was 2.77±0.02 mg GAE/g sample dry base and ferric reducing power was 3.20±0.04 mg GAE/g sample dry base meanwhile Passiflora fruits extract had phytochemical compounds such as alkaloid, phenolic, flavonoids, cardiac glycosides, total phenol was 6.53±1.02 mg GAE/g sample dry base, total flavonoids were 1.56±0.27 mg CE/g sample dry base, DPPH free radical scavenging activity was 1.00±0.15 mg GAE/g sample dry base, and ferric reducing power was 1.12±0.17 mg GAE/g sample dry base. Conclusion: Passiflora leaves extract has higher total phenol, total flavonoid and antioxidant activity measured by DPPH scavenging activity and ferric reducing power value compared with Passiflora fruits extract.

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Optimization of the Extraction and Stability of Antioxidative Peptides from Mackerel (Pneumatophorus japonicus) Protein

BioMed Research International ◽

10.1155/2017/6837285 ◽

2017 ◽

Vol 2017 ◽

pp. 1-14 ◽

Cited By ~ 6

Author(s):

Xueqin Wang ◽

Huahua Yu ◽

Ronge Xing ◽

Xiaolin Chen ◽

Song Liu ◽

...

Keyword(s):

Antioxidant Activity ◽

Protein Hydrolysate ◽

Scientific Basis ◽

Enzyme Concentration ◽

Scavenging Activity ◽

Preparation Conditions ◽

Low Concentrations ◽

Dpph Scavenging Activity ◽

Dpph Scavenging ◽

The Stability

This study optimizes the preparation conditions for mackerel protein hydrolysate (MPH) by response surface methodology (RSM) and investigates the stability of the antioxidant activity of MPHs (<2.5 kDa). The optimal conditions were as follows: enzyme concentration of 1726.85 U/g, pH of 7.00, temperature of 39.55°C, time of 5.5 h, and water/material ratio of 25 : 1, and the maximum DPPH scavenging activity was 79.14%. The MPHs indicated significant cellular antioxidant activity at low concentrations. Furthermore, the temperature and freeze-thaw cycles had little effect on the antioxidative stability while pH had significant effect on the antioxidative stability. In addition, the MPHs were sensitive to the metal ions, such as Fe2+, Fe3+, Zn2+, and Cu2+. Notably, when the concentrations of Fe2+and Fe3+were 5 mM, the DPPH scavenging activities were only 1.1% and 0.6%, respectively; furthermore, Cu2+at a 5 mM concentration could completely inhibit the DPPH scavenging activity of MPHs. In contrast, K+and Mg2+had no notable effect on the antioxidant activity of MPHs. These results may provide a scientific basis for the processing and application of MPHs.

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Antioxidant profiling of Hippophae salicifolia growing in sacred forests of Sikkim, India

Functional Plant Biology ◽

10.1071/fp11016 ◽

2011 ◽

Vol 38 (9) ◽

pp. 697 ◽

Cited By ~ 17

Author(s):

Arvind K. Goyal ◽

Bharat C. Basistha ◽

Arnab Sen ◽

Sushil K. Middha

Keyword(s):

Antioxidant Activity ◽

Sea Buckthorn ◽

Total Phenolic ◽

Scavenging Activity ◽

Methanol Extracts ◽

Hippophae Salicifolia ◽

Total Antioxidant ◽

Dpph Scavenging Activity ◽

Dpph Scavenging ◽

Phenolic And Flavonoid

Hippophae commonly known as sea-buckthorn, is a versatile plant with multipurpose uses including the control of soil erosion, biological nitrogen fixation and medicinal properties. In India, two species of sea-buckthorn; namely, Hippophae salicifolia D. Don and Hippophae rhamnoides L., are very common. H. salicifolia has a shrub-to-tree habit and is restricted to the Himalayan region, whereas H. rhamnoides is bushy, growing at higher altitude in India, and widely distributed in Europe and Asia. In this study, aqueous, methanol and acetone extracts of various parts of female and male plants of H. salicifolia were evaluated for total antioxidant activity, phenolic and flavonoid contents, and correlations were made. The total phenolic and flavonoid content in all the samples ranged from 99 ± 32 to 1459 ± 53 mg gallic acid equivalent g–1, 135 ± 12 to 707 ± 62 mg quercetin equivalent g–1 of dry extract, respectively. The highest free radical (DPPH) scavenging activity was found in methanol extracts of female bark and male leaf. Methanol extracts of male bark and male leaf showed maximum H2O2 activity at a concentration of 1 mg mL–1. Antioxidant activity (FRAP method) ranged from 0.021 to 1.041 at concentrations ranging from 20 to 200 µg mL–1 with a reducing capacity of extracts as water > methanol > acetone. Strong correlation (P < 0.05) was observed between DPPH scavenging activity and total phenolic content from all the extracts under study.

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Chemical Constituents Antioxidant and Anticholinesterasic Activity ofTabernaemontana catharinensis

The Scientific World JOURNAL ◽

10.1155/2013/519858 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 18

Author(s):

Carla Nicola ◽

Mirian Salvador ◽

Adriana Escalona Gower ◽

Sidnei Moura ◽

Sergio Echeverrigaray

Keyword(s):

Mass Spectrometry ◽

Antioxidant Activity ◽

Neurodegenerative Diseases ◽

Chemical Constituents ◽

Ethanolic Extract ◽

Alkaloid Content ◽

Scavenging Activity ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

The present work aimed to analyze the alkaloid content of the ethanolic extract ofTabernaemontana catharinensis(Apocynaceae family) and its fractions as well as to evaluate their antioxidant and anticholinesterasic activities. The analyses of the ethanolic extract ofT. catharinensisby mass spectrometry allowed identifying the presence of the alkaloids 16-epi-affinine, coronaridine-hydroxyindolenine, voachalotine, voacristine-hydroxyindolenine, and 12-methoxy-n-methyl-voachalotine, as well as an alkaloid withm/z385.21 whose spectrum suggests a derivative of voacristine or voacangine. The extract and its alkaloid rich fractions showed antioxidant activity, especially those that contain the alkaloidm/z385.21 or 16-epi-affinine with DPPH scavenging activity (IC50) between 37.18 and 74.69 μg/mL. Moreover, the extract and its fractions exhibited anticholinesterasic activity, particularly the fractions characterized by the presence of 12-methoxy-n-methyl-voachalotine, with IC50= 2.1 to 2.5 μg/mL. Fractions with 16-epi-affinine combined good antioxidant (IC50= 65.59 to 74.69 μg/mL) and anticholinesterasic (IC50= 7.7 to 8.3 μg/mL) activities, representing an option for further studies aimed at treating neurodegenerative diseases.

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