Oxidative Stress Markers in GnRH AgonistaAnd Antagonist Protocols in IVF

SummaryBackground: Our aim was to study the effect of GnRH agonist and antagonist protocols of ovarian stimulation on oxidative stress parameters in serum and the influence of oxidative stress parameters change on the outcome of IVF cycles.Methods: This prospective study included 82 patients who un der went IVF procedures. We determined SOD, MDA and SH groups in serum. Serum samples were obtained between the second and fourth day of the cycle and on the day of HCG administration during ovarian stimulation.Results: Patients were divided into two groups depending on the protocol of stimulation. The mean total and mature oocytes number and number of fertilized oocytes were higher in GnRH agonist group. There was no significant difference in biochemical pregnancy, miscarriage and live -birth rate in both groups. Mean serum SOD was significantly lower, while mean serum MDA and SH groups were significantly higher after ovarian stimulation. Delivery rate was higher in patients without OS while miscarriage rate was higher in patients with OS.Conclusions: Our study confirmed that there is a difference in the concentration of oxidative stress parameters before and after ovarian stimulation. IVF outcome is better in patients without OS after ovarian stimulation. However, the protocol of ovarian stimulation is neither associated with a change in oxidative stress parameters nor with the outcome of ART procedures.

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Homocysteine Levels and Oxidative Stress Parameters in Patients With Adult ADHD

Journal of Attention Disorders ◽

10.1177/1087054714538657 ◽

2016 ◽

Vol 21 (6) ◽

pp. 487-493 ◽

Cited By ~ 10

Author(s):

İbrahim Fatih Karababa ◽

Sermin Nur Savas ◽

Sehabettin Selek ◽

Erdinc Cicek ◽

Esra Ismet Cicek ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin B12 ◽

Adult Adhd ◽

Plasma Homocysteine ◽

Serum Folate ◽

Oxidative Stress Parameters ◽

Oxidative Balance ◽

Significant Difference ◽

And Oxidative Stress ◽

Stress Parameters

Objective: In our study, we aimed to evaluate the plasma homocysteine levels and oxidative stress parameters in patients with adult ADHD (A-ADHD). Method: Blood samples were taken from 32 A-ADHD patients and 32 controls enrolled in the study and plasma homocysteine, serum folate, vitamin B12, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were determined. Results were compared between the groups. Results: Patients’ plasma homocysteine level was significantly lower and serum folate level was significantly higher than the controls. The means of plasma homocysteine and folate levels were statistically significantly different between the groups ( p < .05). However, a statistically significant difference was not detected between groups for mean serum vitamin B12, TAS, TOS, and OSI levels ( p > .05). Conclusion: Our study suggests that oxidative balance is not impaired in A-ADHD, and contrary to other psychiatric disorders, homocysteine levels were found to be lower.

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Hepatoprotective effect of desi and kabuli cultivars of Cicer arietinum L (chick peas) against carbon tetrachloride-induced toxicity in rats

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v19i3.22 ◽

2020 ◽

Vol 19 (3) ◽

pp. 609-615

Author(s):

Mudasir Majeed ◽

Abdullah Ijaz Hussain ◽

Haseeb Anwar ◽

Shahzad Irfan ◽

Shahzad Ali Shahid Chatha ◽

...

Keyword(s):

Oxidative Stress ◽

Carbon Tetrachloride ◽

Cicer Arietinum ◽

Control Group ◽

Serum Samples ◽

Cicer Arietinum L ◽

Oxidative Stress Parameters ◽

High Doses ◽

Ethanol Extracts ◽

Stress Parameters

Purpose: To determine the hepatoprotective potential of ethanol extracts of desi and kabuli cultivars of Cicer arietinum L. (chick peas). Methods: Hepatotoxicity was induced in rats using oral administration of carbon tetrachloride (CCl4). The rats were then orally administered different doses of the ethanol extracts of desi and kabuli cultivars of Cicer arietinum L. for 21 days. Oxidative stress parameters and hepatoprotective profiles were determined in serum samples using standard procedures. The effect of the treatments on liver histology was also determined. Results: Administration of extracts of desi and kabuli cultivars of Cicer arietinum L. to CCl4 treated rats at a dose of 300 mg/kg resulted in a significant (p ≤ 0.05) decrease in oxidative stress parameters, whereas catalase activity significantly increased (p ≤ 0.05); on the other hand, ALT and AST levels were decreased significantly (p ≤ 0.05), when compared to the control group. Conclusion: High doses of Cicer arietinum L (desi and kabuli cultivars) seem to have hepatoprotective and antioxidant effects on CCl4-induced toxicity in rats. This finding underscores the therapeutic importance of Cicer arietinum L. as a plant with hepatoprotective properties. Keywords: Cicer arietinum, Phenolics, Hepatotoxicity, Chick peas, Catalase

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The Effect of Borage (Echium amoenum) on the Mouse Heart and Hematology Parameters

Cardiovascular & Haematological Disorders - Drug Targets ◽

10.2174/1871529x18666181105113617 ◽

2019 ◽

Vol 19 (2) ◽

pp. 154-159 ◽

Cited By ~ 1

Author(s):

Parisa Sadighara ◽

Atefeh Araghi ◽

Behrouz Tajdar-oranj ◽

Leila Peivasteh Roudsari ◽

Afsaneh Mohajer ◽

...

Keyword(s):

Oxidative Stress ◽

Control Group ◽

Mouse Heart ◽

Potential Health ◽

Oxidative Stress Parameters ◽

Significant Difference ◽

High Doses ◽

Hematology Parameters ◽

The Impact ◽

Stress Parameters

Background: There has been considerable interest in the potential health benefits of borage. Little information is available regarding the safety of this plant. The purpose of this study was to evaluate the impact of borage on the mouse heart. Methods: Different amounts of borage extract were injected in mice. The mice were randomly divided into 4 groups including group1 (Control group without injection), group2, 3 and 4 that received 12.5 mg/kg, 25 mg/kg and 50 mg/kg respectively for 28 days. Oxidative stress parameters (lipid peroxidation, total glutathione groups assay and cupric assay) and biochemical (Creatine kinase activity and total cholesterol) and hematology parameters were evaluated. Furthermore, histopathology study was carried out on heart tissues. Results: We found that there was no significant difference in oxidative stress parameters and biochemical parameters between the control group and the groups that received different amounts of borage extract. There were also no changes in histopathology study. In blood parameters, the level of erythrocytes, hematocrit and hemoglobin decreased to 50mg/kg, whereas the level of MCH and MCV decreased in high doses. Conclusion: This article suggested that borage did not cause significant damage to the heart tissue in mice model. In hematology factors, significant changes were observed in erythrocytes and related parameters. Therefore, hematotoxicity of consumption this plant should be considered at high doses.

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Intermittent Fasting Decreases Oxidative Stress Parameters and Increases Total Antioxidant Capacity

Biointerface Research in Applied Chemistry ◽

10.33263/briac125.67636775 ◽

2021 ◽

Vol 12 (5) ◽

pp. 6763-6775

Keyword(s):

Oxidative Stress ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Intermittent Fasting ◽

Sprague Dawley ◽

Oxidative Stress Parameters ◽

Sprague Dawley Rats ◽

Significant Difference ◽

Total Antioxidant ◽

Stress Parameters

Modern lifestyle with an unhealthy routine, including consumption of excessive food and less physical activity, is full of many complications like chronic and degenerative diseases. For driving a healthy life, following a prophylactic style is necessary. The simplest and available way is intermittent fasting (IF). IF can help weight management. IF may lower oxidative stress. Oxidative stress can be determined from the malondialdehyde (MDA) levels and tri acyl glycerol (TAG) in the blood. Also, it increases Total antioxidant capacity (TAC). This study aimed to determine the effect of both protocols, Alternate day fasting (ADF) and time-restricted feeding (TRF), on plasma MDA level, TAG concentration, and TAC level of Sprague Dawley rats. They were randomly assigned to 3 groups, i.e., control group (C), fasting group (ADF), fasting group (TRF). Fasting given in this research was ADF, comprising one day of fasting for 24 hours, alternating with one day of normal feed ad libitum in the span of 12 weeks, including fasting 12 hours for two days a week plus three days in the middle of the month. After 3-months, blood was taken for examination of MDA levels, TAG, and TAC levels. A significant difference among the three groups in MDA levels, TAG and TAC. (p<0.05 for all parameters). Groups conducted on intermittent fasting had lower levels of MDA, TAG concentration, and a high level of TAC. ADF was lower in MDA level and TAG concentration than TRF. ADF was higher in TAC level than TRF. Intermittent fasting (ADF and TRF) decreases the oxidative stress parameters and increases Total antioxidant capacity in Sprague Dawley rats. ADF is more effective than TRF.

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The efficacy of alpha-lipoic acid and/or gabapentin on the oxidant-antioxidant system in patients with diabetic polyneuropathy

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20203424 ◽

2020 ◽

Vol 8 (8) ◽

pp. 2777

Author(s):

Hatice Ferhan Komurcu ◽

Pervin Baran ◽

Ozcan Erel ◽

Omer Anlar

Keyword(s):

Oxidative Stress ◽

Lipoic Acid ◽

Diabetic Polyneuropathy ◽

Serum Levels ◽

Paraoxonase 1 ◽

Stress Index ◽

Alpha Lipoic Acid ◽

Oxidative Stress Parameters ◽

Significant Difference ◽

Stress Parameters

Background: Diabetic peripheral sensorimotor polyneuropathy is the most common complication seen in patients with diabetes mellitus (DM). Oxidant system plays a crucial role in its physiopathology. We investigated the changes in the serum levels of total antioxidant status (TAS), total oxidant status (TOS), paraoxonase-1 (PON1) and oxidative stress index (OSI) to evaluate the antioxidant efficacy of alpha lipoic acid (ALA) and/or gabapentin in patients with diabetic polyneuropathy (DPN).Methods: Sixty-three type 2 DM patients with diabetic polyneuropathy (DPN) were enrolled in the study. Patients with DPN were divided into four groups in terms of their treatment: Group 1 consisted of treatment-naive patients; patients treated with ALA, gabapentin or combination of ALA and gabapentin comprised groups 2, 3, and 4, respectively. The patients received the medications for at least six weeks. Serum levels of TAS, TOS, PON1 and OSI were analyzed.Results: No significant difference was observed between the groups according to the oxidative stress parameters studied.Conclusions: The use of ALA and/or gabapentin in patients with DPN did not significantly affect the oxidative stress parameters, including TAS, TOS, PON1, and OSI.

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The Frequency of PON1 L55M Polymorphism and Measurement of Oxidative Stress Parameters (Superoxide Dismutase, Catalase, Thiol, and Carbonyl) in Neonates with G6PD Deficiency Compared to the Control Group

Journal of Shahid Sadoughi University of Medical Sciences ◽

10.18502/ssu.v27i12.2836 ◽

2020 ◽

Author(s):

Vahide Jamshidi ◽

Vahid Pourshafi ◽

Mahmoud Vakili ◽

Ali Moradi

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Enzyme Activity ◽

G6pd Deficiency ◽

Control Group ◽

Case Group ◽

Genotypic Frequency ◽

Oxidative Stress Parameters ◽

Significant Difference ◽

Stress Parameters

Introduction: Glucose-6-phosphate dehydrogenase deficiency is the most common enzyme disorder. This enzyme involved in maintaining the balance of active oxygen species and its defect causes oxidative damage. PON1 is an HDL-based glycosylated protein that prevents lipid peroxidation. In this study, the prevalence of PON L55M polymorphism in paraoxonase enzyme in neonates with a deficiency in G6PD activity was evaluated, and the level of oxidative stress was measured. Methods: In the present case-control study, 60 infants 2 to 6 months with G6PD enzyme activity deficiency and 60 healthy infants identical in age was selected. Polymorphism examination was done using PCR-RFLP technique, and oxidative stress parameters were measured by spectrophotometry. Chi square and t test statistical analysis of data was performed using SPSS V16 software. Results: The frequency of genotype LL, LM and MM for PON1 L55M was 43.33%, 43.3% and 13.3% and, 35%, 21.6% and 43.3% in control and case group, respectively. Genotypic frequency of LM and MM was significant between control and control groups (P <0.05). The allele frequency between L and M was also significant (P <0.05). Superoxide dismutase enzyme activity and mean carbonyl level comparison did not show a significant difference (P >0.05), but the activity of catalase enzyme and mean level of thiol was showed a significant difference (P <0.05). Conclusion: In the present study, the frequency of LM genotype in neonates with G6PD deficiency was significantly different in comparison to the control group. This frequency is consistent with the results obtained from oxidative stress conditions (significant reduction in the level of thiol and catalase activity).

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The Influence of Oral Multivitamins Supplementation on Selected Oxidative Stress Parameters and Lipid Profiles among Sudanese Patients with Type-2 Diabetes

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2019.137 ◽

2019 ◽

Vol 7 (5) ◽

pp. 775-778 ◽

Cited By ~ 1

Author(s):

Abd Elgadir A. Altoum ◽

Mohammed Y. Abbas ◽

Ahmed L. Osman ◽

Sharique Ahmed ◽

Asaad MA Babker

Keyword(s):

Oxidative Stress ◽

Type 2 Diabetes ◽

Vitamin A ◽

Serum Vitamin ◽

Lipid Profiles ◽

P Value ◽

Oxidative Stress Parameters ◽

Significant Difference ◽

Stress Parameters

AIM: The objective of the current study was to assess the influence of oral multivitamins supplementation on some oxidative stress parameters (serum Vitamin A, C, E, Zinc, Malondialdehyde (MDA)) and lipid profile among Sudanese patients with type- 2 diabetes mellitus (T2DM). MATERIAL AND METHOD: Three hundred Sudanese patients with T2DM and Hundred healthy subjects (control group) were enrolled in this cross-sectional study. Blood was collected after overnight fasting for 10-12 hrs. Fasting plasma glucose (FBG), lipid profiles, Glycosylated haemoglobin (HbA1c%), Serum zinc, Malondialdehyde (MDA), Vitamins A, E, and C levels were measured using standardised laboratory techniques. Data was collected with the help of a structured questionnaire and direct interview. RESULTS: Biochemical parameters of the study population were shown a highly significant difference (P value < 0.05), between the means of serum vitamin A, C, E, Zinc, MDA, HbA1c, triglycerides, HDL, FBG, total cholesterol and LDL. Significant differences in serum vitamin A, C, E, Zinc, MDA, triglycerides, HDL and FBG between people with diabetes who used multivitamins and diabetics who did not use it (P-value < 0.05). CONCLUSION: The study observed a significant increase in serum levels of vitamin A, C & E and other biomarkers parameters in patients with T2DM who take oral multivitamins supplements; such improvement may lead to minimising the diabetic complications. Further studies are needed to explore the possible therapeutic role of multivitamins supplements for T2DM patients.

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Correlation between oxidative stress parameters and left ventricular geometry in patients with chronic heart failure

Acta Facultatis Medicae Naissensis ◽

10.5937/afmnai2003241s ◽

2020 ◽

Vol 37 (3) ◽

pp. 241-251

Author(s):

Sandra Šarić ◽

Tatjana Cvetković ◽

Dejan Petrović ◽

Valentina Mitić ◽

Sanja Stojanović ◽

...

Keyword(s):

Oxidative Stress ◽

Heart Failure ◽

Chronic Heart Failure ◽

Catalase Activity ◽

Left Ventricular ◽

Left Ventricular Geometry ◽

Sh Groups ◽

Oxidative Stress Parameters ◽

Ventricular Geometry ◽

Stress Parameters

Oxidative stress, as one of the pathophysiological mechanisms involved in the reduction of myocardial contractility, stimulates the proliferation of cardiac fibroblasts and the activation of matrix metalloproteinases, which leads to extracellular matrix remodeling. Left ventricular hypertrophy (LVH) is a well-known independent risk factor for cardiovascular events. The aim of this research was to determine the connection between the parameters of oxidative stress and left ventricular geometry indicators in patients with chronic heart failure. The study included 81 subjects diagnosed with heart failure and 68 subjects in the control group. Parameters of myocardial morphology and left ventricular function were measured by echocardiography. Thiobarbituric acid reactive substances (TBARS) content, advanced oxidation protein products (AOPP), total serum SH groups and catalase activity were performed in both groups. The results showed that there were significantly higher concentrations of TBARS, AOPP, and SH groups in patients compared to controls (p < 0.01) and higher concentration of TBARS in patients with concentric hypertrophy compared to patients with normal geometry (p < 0.05). Correlation analysis showed a strong positive correlation between geometry parameters and myocardial function and TBARS and negative correlation between the right ventricule diameter and catalase activity. The results of this study show that oxidative stress parameters are significantly elevated in patients with heart failure, and that concentrations of TBARS and AOPP - the markers of lipid and protein oxidation, strongly correlate with left ventricular geometry parameters.

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Comparison of Biochemical and Oxidative Stress Parameters in Hypo and Hyperthyroid Rat Models

Modern Care Journal ◽

10.5812/modernc.102444 ◽

2020 ◽

Vol 17 (3) ◽

Author(s):

Zohre Najafi ◽

Asghar Zarban ◽

Elham Chamani ◽

Mahdi Honarbakhsh ◽

Gholamreza Sharifzadeh

Keyword(s):

Oxidative Stress ◽

Oxidation State ◽

Thiol Group ◽

The Body ◽

Control Group ◽

Oxidative Stress Parameters ◽

Significant Difference ◽

The Mean ◽

And Oxidative Stress ◽

Stress Parameters

Background: Thyroid functional disorders are relatively common in the general population. Different factors play a role in the development of thyroid disorders. These factors can be associated with the deterioration of the oxidation state of the body and the induction of oxidative stress. Thyroid hormones play an important role in the oxidation state. Objectives: Regarding the role of oxidative stress in different pathologies and the development of various complications, including thyroid disorders, we aimed to compare the biochemical and oxidative stress parameters in hypo and hyperthyroid rat models. Methods: Twenty-one male Wistar rats were assigned into three groups of control, hypothyroid, and hyperthyroid. Hypothyroidism and hyperthyroidism were inducted with PTU (propylthiouracil) and LTX (levothyroxine), respectively. After five weeks of induction, serum biochemical factors and oxidative stress parameters, such as TAC (total antioxidant capacity), MDA (Malondialdehyde), and the thiol group, were measured in these groups. Results: The means of TAC, MDA and UA (uric acid) in the hyperthyroid group were significantly higher than those in the hypothyroid group (P < 0.01, P < 0.01, P < 0.05) but the mean of thiol in the hypothyroid group was significantly higher than that of the hyperthyroid group (P < 0.01). Also, the mean of TAC level in the hypothyroid group was significantly lower than the control group (P < 0.05) and the mean of thiol group in the hypothyroid group was significantly higher than the control group (P < 0.05), but there was no other significant difference between the groups compared to the control group. Among the biochemical parameters, the mean LDH (lactate dehydrogenase) in the hyperthyroid group also showed a significant difference in comparison to the hypothyroid group (P < 0.05), but there was no significant difference between the groups in the mean of the other biochemical parameters. Conclusions: According to the results of this study, hypothyroidism and hyperthyroidism are associated with a disturbance in the oxidation state of the body, and the use of antioxidants can help improve these diseases, especially in hyperthyroidism, due to enhanced metabolism and production of more free radicals.

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