scholarly journals Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

Nanophotonics ◽  
2017 ◽  
Vol 6 (4) ◽  
pp. 681-701 ◽  
Author(s):  
Haley Marks ◽  
Monika Schechinger ◽  
Javier Garza ◽  
Andrea Locke ◽  
Gerard Coté
Keyword(s):  
Raman Spectroscopy ◽  
Point Of Care ◽  
Low Cost ◽  
Diagnostic Testing ◽  
Surface Enhanced Raman Spectroscopy ◽  
World Health ◽  
Great Promise ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

AbstractPoint-of-care (POC) device development is a growing field that aims to develop low-cost, rapid, sensitive in-vitro diagnostic testing platforms that are portable, self-contained, and can be used anywhere – from modern clinics to remote and low resource areas. In this review, surface enhanced Raman spectroscopy (SERS) is discussed as a solution to facilitating the translation of bioanalytical sensing to the POC. The potential for SERS to meet the widely accepted “ASSURED” (Affordable, Sensitive, Specific, User-friendly, Rapid, Equipment-free, and Deliverable) criterion provided by the World Health Organization is discussed based on recent advances in SERS in vitro assay development. As SERS provides attractive characteristics for multiplexed sensing at low concentration limits with a high degree of specificity, it holds great promise for enhancing current efforts in rapid diagnostic testing. In outlining the progression of SERS techniques over the past years combined with recent developments in smart nanomaterials, high-throughput microfluidics, and low-cost paper diagnostics, an extensive number of new possibilities show potential for translating SERS biosensors to the POC.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

scholarly journals Bimetallic Core–Shell Nanoparticles of Gold and Silver via Bioinspired Polydopamine Layer as Surface-Enhanced Raman Spectroscopy (SERS) Platform

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 688 ◽  
Author(s):  
Asli Yilmaz ◽  
Mehmet Yilmaz
Keyword(s):  
Raman Spectroscopy ◽  
Low Cost ◽  
Silver Ions ◽  
Surface Enhanced Raman Spectroscopy ◽  
Core Shell ◽  
Silver Deposition ◽  
Shell Nanoparticles ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Core Shell Nanoparticles

Despite numerous attempts to fabricate the core–shell nanoparticles, novel, simple, and low-cost approaches are still required to produce these efficient nanosystems. In this study, we propose the synthesis of bimetallic core–shell nanoparticles of gold (AuNP) and silver (AgNP) nanostructures via a bioinspired polydopamine (PDOP) layer and their employment as a surface-enhanced Raman spectroscopy (SERS) platform. Herein, the PDOP layer was used as an interface between nanostructures as well as stabilizing and reducing agents for the deposition of silver ions onto the AuNPs. UV-vis absorption spectra and electron microscope images confirmed the deposition of the silver ions and the formation of core–shell nanoparticles. SERS activity tests indicated that both the PDOP thickness and silver deposition time are the dominant parameters that determine the SERS performances of the proposed core–shell system. In comparison to bare AuNPs, more than three times higher SERS signal intensity was obtained with an enhancement factor of 3.5 × 105.

Physically Self-assembled Au Nanorod Arrays for SERS

2006 ◽  
Vol 951 ◽  
Author(s):  
Motofumi Suzuki ◽  
Kaoru Nakajima ◽  
Kenji Kimura ◽  
Takao Fukuoka ◽  
Yasushige Mori
Keyword(s):  
Raman Spectroscopy ◽  
Low Cost ◽  
Major Axis ◽  
Surface Enhanced Raman Spectroscopy ◽  
Nanorod Arrays ◽  
Au Nanorods ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Oblique Deposition ◽  
Selection Of

ABSTRACTWe have demonstrated surface-enhanced Raman spectroscopy on arrays of Au nanorods aligned in line by a dynamic oblique deposition technique. For the light polarized along the major axis of the nanorods, the plasma resonance of the Au nanorods has been tuned to a wavelength suitable for Raman spectroscopy. The Raman scattering on the discrete nanorods is enhanced significantly compared with that on semi continuous Au films. Since the preparation process is physically bottom-up, it is robust in its selection of the materials and is useful in providing the SERS sensors at low cost.

scholarly journals Surface-Enhanced Raman Spectroscopy for Cancer Immunotherapy Applications: Opportunities, Challenges, and Current Progress in Nanomaterial Strategies

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1145 ◽  
Author(s):  
Shuvashis Dey ◽  
Matt Trau ◽  
Kevin M. Koo
Keyword(s):  
Raman Spectroscopy ◽  
Cancer Immunotherapy ◽  
Immune Checkpoint ◽  
Checkpoint Inhibitors ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Checkpoint Receptors

Cancer immunotherapy encompasses a variety of approaches which target or use a patient’s immune system components to eliminate cancer. Notably, the current use of immune checkpoint inhibitors to target immune checkpoint receptors such as CTLA-4 or PD-1 has led to remarkable treatment responses in a variety of cancers. To predict cancer patients’ immunotherapy responses effectively and efficiently, multiplexed immunoassays have been shown to be advantageous in sensing multiple immunomarkers of the tumor microenvironment simultaneously for patient stratification. Surface-enhanced Raman spectroscopy (SERS) is well-regarded for its capabilities in multiplexed bioassays and has been increasingly demonstrated in cancer immunotherapy applications in recent years. This review focuses on SERS-active nanomaterials in the modern literature which have shown promise for enabling cancer patient-tailored immunotherapies, including multiplexed in vitro and in vivo immunomarker sensing and imaging, as well as immunotherapy drug screening and delivery.

scholarly journals A simple and reliable approach for the fabrication of nanoporous silver patterns for surface-enhanced Raman spectroscopy applications

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Angela Capaccio ◽  
Antonio Sasso ◽  
Giulia Rusciano
Keyword(s):  
Raman Spectroscopy ◽  
Inductively Coupled Plasma ◽  
Low Cost ◽  
Cost Effective ◽  
Surface Enhanced Raman Spectroscopy ◽  
Raman Signal ◽  
Plasmonic Nanostructures ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Good Signal

AbstractThe fabrication of plasmonic nanostructures with a reliable, low cost and easy approach has become a crucial and urgent challenge in many fields, including surface-enhanced Raman spectroscopy (SERS) based applications. In this frame, nanoporous metal films are quite attractive, due to their intrinsic large surface area and high density of metal nanogaps, acting as hot-spots for Raman signal enhancement. In this paper, we report a detailed study on the fabrication of nanoporous silver-based SERS substrates, obtained by the application of two successive treatments with an Inductively Coupled Plasma (ICP) system, using synthetic air and Ar as feeding gases. The obtained substrates exhibit a quite broad plasmonic response, covering the Vis–NIR range, and an enhancement factor reaching 6.5 $$\times\, 10^7$$ × 10 7 , estimated by using 4-mercaptobenzoic acid (4-MBA) as probe molecule at 532 nm. Moreover, the substrates exhibit a quite good spatial reproducibility on a centimeter scale, which assures a good signal stability for analytical measurements. Globally, the developed protocol is easy and cost effective, potentially usable also for mass production thanks to the remarkable inter-batches reproducibility. As such, it holds promise for its use in SERS-based sensing platforms for sensitive detection of targets molecules.

scholarly journals Recent Advancement in the Surface-Enhanced Raman Spectroscopy-Based Biosensors for Infectious Disease Diagnosis

2019 ◽  
Vol 9 (7) ◽  
pp. 1448 ◽  
Author(s):  
Logan Hamm ◽  
Amira Gee ◽  
A. Swarnapali De Silva Indrasekara
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Neglected Tropical Diseases ◽  
Point Of Care ◽  
Disease Diagnosis ◽  
Surface Enhanced Raman Spectroscopy ◽  
Biomarker Detection ◽  
Disease Biomarker ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Diagnosis is the key component in disease elimination to improve global health. However, there is a tremendous need for diagnostic innovation for neglected tropical diseases that largely consist of mosquito-borne infections and bacterial infections. Early diagnosis of these infectious diseases is critical but challenging because the biomarkers are present at low concentrations, demanding bioanalytical techniques that can deliver high sensitivity with ensured specificity. Owing to the plasmonic nanomaterials-enabled high detection sensitivities, even up to single molecules, surface-enhanced Raman spectroscopy (SERS) has gained attention as an optical analytical tool for early disease biomarker detection. In this mini-review, we highlight the SERS-based assay development tailored to detect key types of biomarkers for mosquito-borne and bacterial infections. We discuss in detail the variations of SERS-based techniques that have developed to afford qualitative and quantitative disease biomarker detection in a more accurate, affordable, and field-transferable manner. Current and emerging challenges in the advancement of SERS-based technologies from the proof-of-concept phase to the point-of-care phase are also briefly discussed.

ZnO–Ag hybrids for ultrasensitive detection of trinitrotoluene by surface-enhanced Raman spectroscopy

2014 ◽  
Vol 16 (28) ◽  
pp. 14706-14712 ◽  
Author(s):  
Xuan He ◽  
Hui Wang ◽  
Zhongbo Li ◽  
Dong Chen ◽  
Qi Zhang
Keyword(s):  
Raman Spectroscopy ◽  
Low Cost ◽  
High Sensitivity ◽  
Surface Enhanced Raman Spectroscopy ◽  
Ultrasensitive Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Hybrid Substrates ◽  
Tnt Detection

Low-cost SERS sensors were fabricated by 4-ATP-functionalized ZnO–Ag hybrid substrates for TNT detection with high sensitivity, selectivity and reproducibility.

LOC-SERS: towards point-of-care diagnostic of methotrexate

2014 ◽  
Vol 6 (12) ◽  
pp. 3943-3947 ◽  
Author(s):  
I. J. Hidi ◽  
A. Mühlig ◽  
M. Jahn ◽  
F. Liebold ◽  
D. Cialla ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Therapeutic Range ◽  
Point Of Care ◽  
Lab On A Chip ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Methotrexate (MTX), an antifolate antibiotic, is detected using a lab-on-a-chip device via surface enhanced Raman spectroscopy (LOC-SERS) in the therapeutic range of 10 μm to 0.1 μm.

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