Enzymatic Synthesis o f Sinapoyl-L-M alate from 1-Sinapoylglucose and L-M alate by a Protein Preparation from Raphanus sativus Cotyledons

1980 ◽  
Vol 35 (9-10) ◽  
pp. 835-837 ◽  
Author(s):  
Norbert Tkotz ◽  
Dieter Strack
Keyword(s):  
Enzymatic Synthesis ◽  
Raphanus Sativus ◽  
Protein Preparation ◽  
Sugar Derivatives ◽  
Acyl Donors ◽  
First Time ◽  
Acylation Reactions

Abstract Protein preparations from cotyledons of Raphanus sativus transfer the sinapoyl moiety of 1 -sinapoylglucose to L-malate to form sinapoyl-L-malate. To our knowledge this is the first time that this type of reaction has been found to be involved in phenylpropanoid depside formation. Most acylation reactions have so far been demonstrated to depend on acyl-CoAs and only one example is known describing 1-O-acyl sugar derivatives as acyl donors.

Enzymatic Synthesis of Glucose‐ and Xylose Laurate Esters Using Different Acyl Donors, Higher Substrate Concentrations, and Membrane Assisted Solvent Recovery

2020 ◽  
pp. 2000225
Author(s):  
Marta Martinez‐Garcia ◽  
Winnie Dejonghe ◽  
Lieve Cauwenberghs ◽  
Miranda Maesen ◽  
Karolien Vanbroekhoven ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Solvent Recovery ◽  
Acyl Donors ◽  
Substrate Concentrations

Enantioselective Enzymatic Synthesis of ( R )‐Phenyl Alkyl Esters and Their Analogue Amides using Fatty Acids as Green Acyl Donors.

2021 ◽  
Vol 6 (48) ◽  
pp. 13941-13946
Author(s):  
Nour ElHouda Benamara ◽  
Mounia Merabet‐Khelassi ◽  
Samia Guezane Lakoud ◽  
Louisa Aribi‐Zouioueche ◽  
Olivier Riant
Keyword(s):  
Fatty Acids ◽  
Enzymatic Synthesis ◽  
Alkyl Esters ◽  
Acyl Donors

Enzymatic Synthesis of 1-Sinapoylglucose from Free Sinapic Acid and UDP-Glucose by a Cell-Free System from Raphanus sativus Seedlings

1980 ◽  
Vol 35 (3-4) ◽  
pp. 204-208 ◽  
Author(s):  
Dieter Strack
Keyword(s):  
Phenolic Acids ◽  
Enzymatic Synthesis ◽  
Raphanus Sativus ◽  
High Performance ◽  
Sinapic Acid ◽  
Carboxyl Group ◽  
Ph Optimum ◽  
Free System ◽  
Sh Group ◽  
A Cell

Abstract Protein extracts from seedlings of Raphanus sativus catalyze the transfer of the glucosyl moiety of UDP-glucose to the carboxyl group of phenolic acids. Enzymatic activity was determined spectrophotometrically by measuring the increase in absorbance at 360 nm and/or by the aid of high performance liquid chromatography (HPLC). From 12 phenolic acids tested as acceptors, sinapic acid was by far the best substrate. The glucosyltransfer to sinapic acid has a pH optimum near 7 and requires as SH group for activity, p-Chloromercuribenzoate (PCMB) inhibits activity, which can be restored by the addition of dithiothreitol (DTT). The formation of 1-sinapoylglucose was found to be a reversible reaction, since the addition of UDP results in a breakdown of the ester.

scholarly journals Enantioselective Transesterification of Allyl Alcohols with (E)-4-Arylbut-3-en-2-ol Motif by Immobilized Lecitase™ Ultra

Catalysts ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 798
Author(s):  
Aleksandra Leśniarek ◽  
Anna Chojnacka ◽  
Radosław Drozd ◽  
Magdalena Szymańska ◽  
Witold Gładkowski
Keyword(s):  
High Activity ◽  
Cyanogen Bromide ◽  
Kinetic Resolution ◽  
Negative Effects ◽  
Subsequent Reaction ◽  
Wide Range ◽  
Allyl Alcohols ◽  
Reaction Cycles ◽  
Acyl Donors ◽  
First Time

Lecitase™ Ultra was immobilized on four different supports and tested for the first time as the biocatalyst in the kinetic resolution of racemic allyl alcohols with the (E)-4-arylbut-3-en-2-ol system in the process of transesterification. The most effective biocatalyst turned out to be the enzyme immobilized on agarose activated with cyanogen bromide (LU-CNBr). The best results (E > 200, ees and eep = 95–99%) were obtained for (E)-4-phenylbut-3-en-2-ol and its analog with a 2,5-dimethylphenyl ring whereas the lowest ee of kinetic resolution products (90%) was achieved for the substrate with a 4-methoxyphenyl substituent. For all substrates, (R)-enantiomers were esterified faster than their (S)-antipodes. The results showed that LU-CNBr is a versatile biocatalyst, showing high activity and enantioselectivity in a wide range of organic solvents in the presence of commonly used acyl donors. High operational stability of LU-CNBr allows it to be reused in three subsequent reaction cycles without negative effects on the efficiency and enantioselectivity of transesterification. This biocatalyst can become attractive to the commercial lipases in the process of the kinetic resolution of allyl alcohols.

scholarly journals Poly(butylene succinate-co-ε-caprolactone) Copolyesters: Enzymatic Synthesis in Bulk and Thermal Properties

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2679
Author(s):  
María Núñez ◽  
Sebastián Muñoz-Guerra ◽  
Antxon Martínez de Ilarduya
Keyword(s):  
Enzymatic Synthesis ◽  
Biomedical Applications ◽  
Eutectic Point ◽  
Polymerization Reaction ◽  
Butylene Succinate ◽  
Molecular Weights ◽  
Melting Temperatures ◽  
Random Microstructure ◽  
Metallic Catalyst ◽  
First Time

This work explores for the first time the enzymatic synthesis of poly(butylene-co-ε-caprolactone) (PBSCL) copolyesters in bulk using commercially available monomers (dimethyl succinate (DMS), 1,4-butanediol (BD), and ε-caprolactone (CL)). A preliminary kinetic study was carried out which demonstrated the higher reactivity of DMS over CL in the condensation/ring opening polymerization reaction, catalyzed by Candida antarctica lipase B. PBSCL copolyesters were obtained with high molecular weights and a random microstructure, as determined by 13C NMR. They were thermally stable up to 300 °C, with thermal stability increasing with the content of CL in the copolyester. All of them were semicrystalline, with melting temperatures and enthalpies decreasing up to the eutectic point observed at intermediate compositions, and glass transition temperatures decreasing with the content of CL in the copolyester. The use of CALB provided copolyesters free from toxic metallic catalyst, which is very useful if the polymer is intended to be used for biomedical applications.

scholarly journals Enzymatic synthesis of an electrospinnable poly(butylene succinate-co-dilinoleic succinate) thermoplastic elastomer

RSC Advances ◽  
2017 ◽  
Vol 7 (34) ◽  
pp. 21258-21267 ◽  
Author(s):  
Agueda Sonseca ◽  
Miroslawa El Fray
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Enzymatic Synthesis ◽  
Diphenyl Ether ◽  
Thermoplastic Elastomer ◽  
Candida Antarctica ◽  
Two Stage ◽  
Butylene Succinate ◽  
Diethyl Succinate ◽  
First Time

Candida antarcticalipase B was successfully employed for the first time as a biocatalyst to obtain high molecular weight PBS : DLS copolyesterviaa two-stage method in diphenyl ether from diethyl succinate, 1,4-butanediol, and dimer linoleic diol.

Enzymatic synthesis of benzyl benzoate using different acyl donors: Comparison of solvent-free reaction techniques

2020 ◽  
Vol 92 ◽  
pp. 261-268
Author(s):  
Alessandra Cristina de Meneses ◽  
Manuela Balen ◽  
Elaine de Andrade Jasper ◽  
Ilka Korte ◽  
Pedro Henrique Hermes de Araújo ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Solvent Free ◽  
Benzyl Benzoate ◽  
Acyl Donors ◽  
Solvent Free Reaction

Chemical Constituents Study of Weixian Turnip (Raphanus sativus L.)

2014 ◽  
Vol 941-944 ◽  
pp. 1036-1039
Author(s):  
Gui Feng Li ◽  
Li Gang Deng ◽  
Hong Zhang ◽  
Ping Juan Zhao ◽  
Hui Wang ◽  
...  
Keyword(s):  
Raphanus Sativus ◽  
Chemical Constituents ◽  
Chemical Properties ◽  
Biologically Active ◽  
Isolation And Characterization ◽  
Raphanus Sativus L ◽  
Physical And Chemical ◽  
First Time ◽  
Active Effects

Objective: Cruciferous herb plant Weixian Radish (Raphanus Sativus L.), also known as Lai Fu, is a kind of popularly eatable fruit and vegetable. The present paper describes the isolation and characterization of two compounds isolated from Weixian Radish. Methods: The methanol extraction was separated by column chromatography. Obtained compounds were identified with physical and chemical properties and NMR. Results: Two compounds were isolated from the extract of Raphanus Sativus L. for the first time and their structures were identified as β-sitosterol and 1-O-(β-D-glucopyranosyl)-(2S, 3S, 4R, 8E)-2-[(2'R)-2'-hydroxyltetracos-15'-enoylamino]-8-octa-decene-1, 3, 4-triol, which have many biologically active effects.

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