Chemical Constituents Study of Weixian Turnip (Raphanus sativus L.)

2014 ◽  
Vol 941-944 ◽  
pp. 1036-1039
Author(s):  
Gui Feng Li ◽  
Li Gang Deng ◽  
Hong Zhang ◽  
Ping Juan Zhao ◽  
Hui Wang ◽  
...  
Keyword(s):  
Raphanus Sativus ◽  
Chemical Constituents ◽  
Chemical Properties ◽  
Biologically Active ◽  
Isolation And Characterization ◽  
Raphanus Sativus L ◽  
Physical And Chemical ◽  
First Time ◽  
Active Effects

Objective: Cruciferous herb plant Weixian Radish (Raphanus Sativus L.), also known as Lai Fu, is a kind of popularly eatable fruit and vegetable. The present paper describes the isolation and characterization of two compounds isolated from Weixian Radish. Methods: The methanol extraction was separated by column chromatography. Obtained compounds were identified with physical and chemical properties and NMR. Results: Two compounds were isolated from the extract of Raphanus Sativus L. for the first time and their structures were identified as β-sitosterol and 1-O-(β-D-glucopyranosyl)-(2S, 3S, 4R, 8E)-2-[(2'R)-2'-hydroxyltetracos-15'-enoylamino]-8-octa-decene-1, 3, 4-triol, which have many biologically active effects.

scholarly journals Industrial Gelatin from Leather Chrome Shavings Wastes

2021 ◽  
Vol 2 (10) ◽  
pp. 1035-1043
Author(s):  
Nabil H Elsayed ◽  
Ghada M. Taha ◽  
Ola A. Mohamed
Keyword(s):  
Chemical Properties ◽  
Added Value ◽  
Physical And Chemical Properties ◽  
Isolation And Characterization ◽  
Hydrolysis Products ◽  
Alkali Hydrolysis ◽  
Hydrolysis Conditions ◽  
Physical And Chemical ◽  
And Chemical Properties

A step towards minimizing the environmental pollution of leather tanning , leather chrome shavings wastes were treated with Li2CO3 to extract technical or industrial gelatin as an added value material. Isolation and characterization of gelatin obtained from chrome-tanned shavings were done. The alkali hydrolysis products obtained, showed good physical and chemical properties in terms of gel strength, swelling and thermal stability. The optimum hydrolysis conditions using Li2CO3 were found to be 5 hr. extraction at 80°C, swelling time of one day and pH 9.5. The yield was over one third of the original starting waste material.

scholarly journals Isolation and characterization of the chemical constituents of Sonchus wightianus of Nepalese origin

2013 ◽  
Vol 28 ◽  
pp. 115-120 ◽  
Author(s):  
Susan Joshi ◽  
Tej Narayan Poudel
Keyword(s):  
Fatty Acids ◽  
Methyl Ester ◽  
Chemical Constituents ◽  
Chemical Society ◽  
Phytochemical Screening ◽  
Isolation And Characterization ◽  
First Time

Phytochemical screening of Sonchus wightianus of Nepalese origin were carried out. Phytochemical screening showed the presence of fatty acids, triterpenes, sterols, polyphenols, emodins, quinones, glycosides, polyose and anthracenosides. Compounds β-sitosterol, β-sitosterol glycoside, 1-Hexacosanol and Hexadecanoic methyl ester were isolated.1-Hexacosanol and Hexadecanoic methyl ester are isolated for the first time from Sonchus wightianus. DOI: http://dx.doi.org/10.3126/jncs.v28i0.8119 Journal of Nepal Chemical Society Vol. 28, 2011 Page: 115-120 Uploaded Date: May 24, 2013

Chemical Constituents and Antibacterial Activity of Cissus Aralioides.

2020 ◽  
Vol 17 ◽  
Author(s):  
Balogun Olaoye Solomon ◽  
Ajayi Olukayode Solomon ◽  
Owolabi Temitayo Abidemi ◽  
Oladimeji Abdulkarbir Oladele ◽  
Liu Zhiqiang
Keyword(s):  
Plant Extract ◽  
Chemical Constituents ◽  
Ethyl Acetate Fraction ◽  
Antibacterial Activities ◽  
Sub Saharan Africa ◽  
Chromatographic Purification ◽  
Whole Plant ◽  
Sub Saharan ◽  
First Time

: Cissus aralioides is a medicinal plant used in sub-Saharan Africa for treatment of infectious diseases; however the chemical constituents of the plant have not been investigated. Thus, in this study, attempt was made at identifying predominant phytochemical constituents of the plant through chromatographic purification and silylation of the plant extract, and subsequent characterization using spectroscopic and GC-MS techniques. The minimum inhibitory concentration (MICs) for the antibacterial activities of the plant extract, chromatographic fractions and isolated compounds were also examined. Chromatographic purification of the ethyl acetate fraction from the whole plant afforded three compounds: β-sitosterol (1), stigmasterol (2) and friedelin (3). The phytosterols (1 and 2) were obtained together as a mixture. The GC-MS analysis of silylated extract indicated alcohols, fatty acids and sugars as predominant classes, with composition of 24.62, 36.90 and 26.52% respectively. Results of MICs indicated that friedelin and other chromatographic fractions had values (0.0626-1.0 mg/mL) comparable with the standard antibiotics used. Characterization of natural products from C. aralioides is being reported for the first time in this study.

Isolation and HPLC Determination of the Chemical Components of Gentianella acuta (Michx.) Hulten

2018 ◽  
Vol 15 (1) ◽  
pp. 21-33
Author(s):  
Ying Wei ◽  
Yongqiao Liu ◽  
Yifan Hele ◽  
Weiwei Sun ◽  
Yang Wang ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
High Speed ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Gradient Elution ◽  
Chemical Components ◽  
Isolation And Characterization ◽  
Major Chemical ◽  
Main Components ◽  
First Time

Background: Gentianella acuta (Michx.) Hulten is an important type of medicinal plant found in several Chinese provinces. It has been widely used in folk medicine to treat various illnesses. However, there is not enough detailed information about the chemical constituents of this plant or methods for their content determination. Objective: The focus of this work is the isolation and characterization of the major chemical constituents of Gentianella acuta, and developing an analytical method for their determination. Methods: The components of Gentianella acuta were isolated using (1) ethanol extraction and adsorption on macroporous resin. (2) and ethyl acetate extraction and high speed countercurrent chromatography. A HPLC-DAD method was developed using a C18 column and water-acetonitrile as the mobile phase. Based on compound polarities, both isocratic and gradient elution methods were developed. Results: A total of 29 compounds were isolated from this plant, of which 17 compounds were isolated from this genus for the first time. The main components in this plant were found to be xanthones. The HPLC-DAD method was developed and validated for their determination, and found to show good sensitivity and reliability. Conclusion: The results of this work add to the limited body of work available on this important medicinal plant. The findings will be useful for further investigation and development of Gentianella acuta for its valuable medicinal properties.

scholarly journals Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Saleem Farooq ◽  
Ruqeya Nazir ◽  
Shabir Ahmad Ganai ◽  
Bashir Ahmad Ganai
Keyword(s):  
Specific Activity ◽  
Temperature Range ◽  
Psychrotrophic Bacteria ◽  
Isolation And Characterization ◽  
Cold Active ◽  
Active Protease ◽  
Quantitative Screening ◽  
First Time ◽  
Low K

AbstractAs an approach to the exploration of cold-active enzymes, in this study, we isolated a cold-active protease produced by psychrotrophic bacteria from glacial soils of Thajwas Glacier, Himalayas. The isolated strain BO1, identified as Bacillus pumilus, grew well within a temperature range of 4–30 °C. After its qualitative and quantitative screening, the cold-active protease (Apr-BO1) was purified. The Apr-BO1 had a molecular mass of 38 kDa and showed maximum (37.02 U/mg) specific activity at 20 °C, with casein as substrate. It was stable and active between the temperature range of 5–35 °C and pH 6.0–12.0, with an optimum temperature of 20 °C at pH 9.0. The Apr-BO1 had low Km value of 1.0 mg/ml and Vmax 10.0 µmol/ml/min. Moreover, it displayed better tolerance to organic solvents, surfactants, metal ions and reducing agents than most alkaline proteases. The results exhibited that it effectively removed the stains even in a cold wash and could be considered a decent detergent additive. Furthermore, through protein modelling, the structure of this protease was generated from template, subtilisin E of Bacillus subtilis (PDB ID: 3WHI), and different methods checked its quality. For the first time, this study reported the protein sequence for psychrotrophic Apr-BO1 and brought forth its novelty among other cold-active proteases.

scholarly journals Characterization of a Novel Fructosyltransferase from Lactobacillus reuteri That Synthesizes High-Molecular-Weight Inulin and Inulin Oligosaccharides

2002 ◽  
Vol 68 (9) ◽  
pp. 4390-4398 ◽  
Author(s):  
S. A. F. T. van Hijum ◽  
G. H. van Geel-Schutten ◽  
H. Rahaoui ◽  
M. J. E. C. van der Maarel ◽  
L. Dijkhuizen
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Lactobacillus Reuteri ◽  
Bacterial Strains ◽  
Potential Health ◽  
Isolation And Characterization ◽  
A Cell ◽  
Encoding Gene ◽  
First Time

ABSTRACT Fructosyltransferase (FTF) enzymes produce fructose polymers (fructans) from sucrose. Here, we report the isolation and characterization of an FTF-encoding gene from Lactobacillus reuteri strain 121. A C-terminally truncated version of the ftf gene was successfully expressed in Escherichia coli. When incubated with sucrose, the purified recombinant FTF enzyme produced large amounts of fructo-oligosaccharides (FOS) with β-(2→1)-linked fructosyl units, plus a high-molecular-weight fructan polymer (>107) with β-(2→1) linkages (an inulin). FOS, but not inulin, was found in supernatants of L. reuteri strain 121 cultures grown on medium containing sucrose. Bacterial inulin production has been reported for only Streptococcus mutans strains. FOS production has been reported for a few bacterial strains. This paper reports the first-time isolation and molecular characterization of (i) a Lactobacillus ftf gene, (ii) an inulosucrase associated with a generally regarded as safe bacterium, (iii) an FTF enzyme synthesizing both a high molecular weight inulin and FOS, and (iv) an FTF protein containing a cell wall-anchoring LPXTG motif. The biological relevance and potential health benefits of an inulosucrase associated with an L. reuteri strain remain to be established.

A review on phyto-pharmacology of Oxalis corniculata

2021 ◽  
Vol 24 ◽  
Author(s):  
Iqra Sarfraz ◽  
Azhar Rasul ◽  
Ghulam Hussain ◽  
Muhammad Ajmal Shah ◽  
Bushra Nageen ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Current Effort ◽  
Isolation And Characterization ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Anti Fungal ◽  
Warm Temperate

: Oxalis corniculata (Oxalidaceae) is a small decumbent and delicate appearing medicinal herb flourishing in warm temperate and tropical domains such as Pakistan and India. Main bioactive chemical constituents of Oxalis plant include several alkaloids, flavonoids, terpenoids, cardiac glycosides, saponins, phlobatannins along with steroids. Due to its polyphenolic, glycosides and flavonoid profile, it is proved to be protective in numerous ailments and exhibit various biological activities such as anti-fungal, anti-cancer, anti-oxidant, anti-bacterial, anti-diabetic, and cardioprotective. Moreover, bioactive phytochemicals from this plant possess significant wound healing potential. Our current effort intends to emphasize on the immense significance of this plant species, which have not been the subject matter of clinical trials and effective pharmacological studies, even though its favored usage has been stated. This review proposes that Oxalis corniculata possess potential for the cure of various diseases, however, further researches on isolation and characterization of bioactive compounds along with pre-clinical trials are compulsory to figure out its pharmacological applications.

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