Cytotoxic Activity of Orsellinates

AbstractThe series of 2,4-dihydroxy-6-methylbenzoates 2-10 (methyl to hexyl orsellinates) prepared by alcoholysis of lecanoric acid (1) - a natural product from the lichen Parmotrema tinctorum (Nyl.) Hale - was submitted to the brine shrimp lethality test (BST), which was also performed for 2,4-dihydroxy-6-methylbenzoic acid (11) (orsellinic acid) and the derivative ethyl-2-hydroxy-4-methoxy-6-methylbenzoate (12) (4-methoxy-ethyl orsellinate), in order to detect new substances with probable antineoplasic activity. Results showed that chain elongation - increase in lipophilicity (log P) - causes a rise in the cytotoxic activity of orsellinates. Hexyl orsellinate (7) showed the highest cytotoxic activity (LC50 = 31 μм). A correlation between lipophilicity (log P) and cytotoxic activity (log 1/LC50) is presented. Compounds with ramified chains - iso-propyl, sec-butyl and tert-butyl orsellinates (8-10) - were less active than those with the correspondent linear chain. The activities presented by 4-methoxy-ethyl orsellinate (12) and ethyl orsellinate (3) suggest that the hydroxy group at the C-4 position causes effect in the cytotoxic activity of orsellinates against Artemia salina.

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UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

JURNAL PESISIR DAN LAUT TROPIS ◽

10.35800/jplt.7.3.2019.26017 ◽

2019 ◽

Vol 7 (3) ◽

pp. 265

Author(s):

Faradila Y. Karim ◽

Nickson J Kawung ◽

Billy Th. Wagey

Keyword(s):

Cytotoxic Activity ◽

Toxicity Test ◽

Brine Shrimp ◽

Bioactive Compound ◽

Artemia Salina ◽

Probit Analysis ◽

Thalassia Hemprichii ◽

Brine Shrimp Lethality ◽

A Value ◽

Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)

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A Cytotoxic Activity of Tahongai (Kleinhovia hospita Linn.) Leaves Extracts Using Brine Shrimp Lethality Test

Science & Technology Indonesia ◽

10.26554/sti.2019.4.3.60-63 ◽

2019 ◽

Vol 4 (3) ◽

pp. 60

Author(s):

Indah Solihah ◽

Herlina Herlina ◽

Riana Sari Puspita Rasyid ◽

Tri Suciati ◽

Khairunnisa Khairunnisa

Keyword(s):

Statistical Analysis ◽

Ethyl Acetate ◽

Cytotoxic Activity ◽

Brine Shrimp ◽

Ethanol Extract ◽

Artemia Salina ◽

Brine Shrimp Lethality ◽

Ethanol Extracts

Tahongai (Kleinhovia hospita Linn.) leaves had been known contain alkaloids, flavonoids, saponins, steroids, and tannins. At Komering, South Sumatera tribe, tahongai leaves had been known to treat tumor, cancer, polyps, acne, and dysmenorrhea. The study of cytotoxic activity of tahongai bark and stem were done. This study aims to determine the cytotoxic activity of tahongai leaves extracts using BSLT method. Tahongai leaves were extracted using gradual maceration with n-hexane, ethyl acetate, and ethanol 96%. Each extract was tested cytotoxic activity towards Artemia salina L. larvae. The yield of n-hexane, ethyl acetate, and ethanol extracts are 2,686%, 7,033%, and 7,933% respectively. Ethanol extract of tahongai leaves had the best cytotoxic activity with lethality value 76,667% at 500ppm. Statistical analysis with two way ANOVA showed extract and concentration had a significant (p<0,05) effect on larvae lethality percentage.

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UJI AKTIVITAS SITOTOKSIK EKSTRAK METANOL BUAH KETUMBAR (Coriandrum sativum Linn) TERHADAP ARTEMIA SALINA LEACH DENGAN UJI BSLT (Brine Shrimp Lethality Test)

Jurnal Riset Kefarmasian Indonesia ◽

10.33759/jrki.v2i3.72 ◽

2020 ◽

Vol 2 (3) ◽

pp. 137-146

Author(s):

Mega Yulia ◽

Rani Anggraini ◽

Farizal Farizal

Keyword(s):

Cytotoxic Activity ◽

Brine Shrimp ◽

Methanol Extract ◽

Artemia Salina ◽

Test Method ◽

Coriandrum Sativum ◽

Brine Shrimp Lethality ◽

Activity Test ◽

Anti Cancer ◽

Expected Benefits

Coriander is a common herb can be used as traditional medicine. One of the expected benefits of Coriander fruits is an anti-cancer. This study aims to determine the cytotoxic activity of Coriander extract (Coriandrum sativum Linn) on shrimp larvae (Artemia salina Leach) with the BSLT (Brine Shrimp Lethality Test) method. Coriander fruits was extracted using methanol solvent and maceration method, then concentrated it with a rotary evaporator until a thick extract. Cytotoxic activity test used methanol extract with several concentration : 1,000 ppm, 100 ppm, 10 ppm, and 1 ppm. The result of this research reveal that methanol extract of Coriander fruits has cytotoxic activity with LC50 value is 32.35 ppm.

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THE CYTOTOXIC ACTIVITY OF N-HEXANE EXTRACT OF KERSEN(muntingia calabura linn.) LEAVES USING THE BRINE SHRIMP LETHALITY TEST (BSLT) METHOD

Jurnal Natural ◽

10.24815/jn.v16i2.5124 ◽

2016 ◽

Vol 16 (2) ◽

pp. 37-44

Author(s):

Irma Sari ◽

Titania Miranda ◽

Sadli Sadli

Keyword(s):

Cytotoxic Activity ◽

Brine Shrimp ◽

Artemia Salina ◽

Probit Analysis ◽

Hexane Extract ◽

Water Soluble ◽

Extractive Content ◽

Brine Shrimp Lethality ◽

Lc50 Value ◽

Muntingia Calabura

The cytotoxic activity experiment of n-hexane extract of kersen (Muntingia calabura Linn.) leaves has been carried out using the Brine Shrimp Lethality Test (BSLT) method. The purpose of this research is to identify the secondary metabolites, extract characterize, and determine the LC50 value of the extract against larvae shrimp Artemia salina Leach. Screening result showed that n-hexane extract of kersen leaves contain triterpenoid. Extract characterization showed the results water content of 2.59±0.18 %w/w, water soluble extractive content of 1.9%±0.19 %w/w, the ethanol soluble extractive content of 17.41±1,87 %w/w and total ash value 0.25% w/w. Extract showed LC50 value is 278,72 ppm were calculated by probit analysis. The LC50 value indicated that the n-hexane extract of kersen leaves potentially has cytotoxic activity.

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Cytotoxic Activity of Two Limonoids Isolated From Swietenia mahagoni by Using Brine Shrimp Lethality Bioassay

Bangladesh Journal of Physiology and Pharmacology ◽

10.3329/bjpp.v24i1.5729 ◽

1970 ◽

pp. 1-6

Author(s):

AKM Shahidur Rahman ◽

AK Azad Chowdhury ◽

Hossain Reza ◽

Mohammad Shawkat Ali ◽

Manzurur Rahman Shah Choudhury ◽

...

Keyword(s):

Cytotoxic Activity ◽

Key Words ◽

Column Chromatography ◽

Brine Shrimp ◽

Meoh Extract ◽

Brine Shrimp Lethality ◽

Swietenia Mahagoni

Solvent partitioning followed by column chromatography of the MeOH extract of the seeds of Swietenia mahagoni afforded two limonoids, swietenolide (compound 1) and 2-hydroxy-3-O-tigloylswietenolide (compound 2), later one is new compound. The compounds were identified by spectroscopic means. The cytotoxic activity of these compounds was assessed by using the conventional brine shrimp lethality bioassay. While both compounds were found to have moderate cytotoxic activity, compound 2 displayed overall more potent activity than compound 1. Key words: Cytotoxic; Limonoid; Swietenia mahagoni; Brine shrimp lethality bioassay DOI: 10.3329/bjpp.v24i1.5729Bangladesh J Physiol Pharmacol 2008; 24(1&2) : 1-6

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KAJIAN TOKSISITAS DARI TUBUH BUAH Ganoderma lucidum DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST)

Scripta Biologica ◽

10.20884/1.sb.2014.1.1.22 ◽

2014 ◽

Vol 1 (1) ◽

pp. 32

Author(s):

Ross Nurul Rohmah ◽

Nuniek Ina Ratnaningtyas ◽

Ari Asnani

Keyword(s):

Toxic Effect ◽

Fruiting Body ◽

Brine Shrimp ◽

Ganoderma Lucidum ◽

Ethanol Extract ◽

Artemia Salina ◽

Phytochemical Analysis ◽

Brine Shrimp Lethality ◽

One Stage ◽

Body Extract

Ganoderma lucidum is polyporus fungi from Basidiomycetes which can be used as traditional medicines. Toxicity test with Brine Shrimp Lethality Test (BST) method using Artemia salina was conducted to find out toxic effect of G.lucidum. An extract would have toxic effect if the LC50 < 1000 µg/ml. The aims of this experiment were to know about the toxicity level from G.lucidum with Brine Shrimp Lethality Test (BST) method and determined the concentration of fruiting body extract of G.lucidum which had the best toxicity effect in LC50. There were two extraction methods used in this experiment, first one stage extraction and then multilevel extraction with hexane, ethyl acetate, and ethanol. Each solvent was made in 1000 ppm, 500 ppm, 250 ppm, and 125 ppm. This process was repeated for three times. This experiment used G.lucidum extract which was tested to A.salina and secunder compound metabolit test from the most toxic G. lucidum was done with phytochemical analysis. The result indicated that fruiting body extract from G.lucidum could kill A.salina because all extract was positively contained alkaloid and terpenoid but negatively contained flavonoid. Fruiting body extract from G.lucidum which had lowest LC50 wass ethyl asetat extract with one stage extraction in concentration 53,70 ppm and highest LC50 was ethanol extract with multilevel extraction in concentration 501,18 ppm.

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Skrining antikanker menggunakan metode BST (Brine Shrimp Lethality Test) pada ekstrak metanol daun saga (Abrus precatorius L.) dengan partisi etanol

Riset Informasi Kesehatan ◽

10.30644/rik.v7i1.129 ◽

2018 ◽

Vol 7 (1) ◽

pp. 61

Author(s):

Kurnia Ritma Dhanti

Keyword(s):

Brine Shrimp ◽

Artemia Salina ◽

Brine Shrimp Lethality ◽

Abrus Precatorius

Latar belakang : Pengembangan senyawa antikanker dari bahan alami perlu dilakukan untuk meminimalisir efek samping dari penanganan penyakit kanker yang saat ini banyak dilakukan. Suatu senyawa dapat diketahui potensi antikankernya dengan pendekatan menggunakan metode BST (Brine Shrimp Lethality Test). Ekstrak metanol daun saga (Abrus precatorius L.) bersifat toksik terhadap larva A. salina Leach. Tujuan : Mengetahui bagian teraktif dari ekstrak metanol daun saga yang dipartisi (dipisahkan) menggunakan pelarut etanol. Metode : Ekstrak metanol daun saga dipartisi dengan pelarut etanol hingga terbentuk bagian larut dan tidak larut. Kedua bagian tersebut diuji menggunakan metode BST dengan 5 kali ulangan dan 3 replikasi yang masing-masing menggunakan 10 ekor larva A.salina. Hasil : Dari perhitungan didapatkan nilai LC50 bagian larut etanol sebesar 144,544 ppm sedangkan nilai LC50 bagian tidak larut etanol sebesar 151,356 ppm. Kesimpulan : Bagian larut etanol menyebabkan persentase kematian yang lebih tinggi daripada bagian tidak larut. Nilai LC50 bagian larut etanol lebih rendah dibanding bagian tidak larutnya. Semakin rendah nilai LC50 senyawa, maka semakin berpotensi pula untuk dikembangkan sebagai agen antikanker. Kata kunci : toksisitas, Abrus precatorius L., Artemia salina Leach., antikanker, partisi.

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Pengaruh Penggunaan Jenis Pelarut dalam Uji Sitotoksistas Metode Brine Shrimp Lethality Test (BSLT) pada Wound Dressing Kolagen-Kitosan

al-Kimiya ◽

10.15575/ak.v8i1.10277 ◽

2021 ◽

Vol 8 (1) ◽

pp. 15-20

Author(s):

Ary Andini ◽

Endah Prayekti ◽

Fadillah Triasmoro ◽

Indah Nur Kamaliyah

Keyword(s):

Brine Shrimp ◽

Wound Dressing ◽

Artemia Salina ◽

Lethal Concentration ◽

Brine Shrimp Lethality

Kolagen dan kitosan dapat digunakan sebagai bahan pembalut luka karena memiliki karakteristik yang baik. Namun, pembalut luka kolagen-kitosan perlu dilakukan uji sitotoksisitas sebelum diaplikasikan secara in vivo, seperti Brine Shrimp Lethally Test (BSLT). Pembalut luka kolagen-kitosan tidak dapat larut dalam Dimetil Sulfoksida (DMSO) dan aquadest dengan mudah, oleh karena itu perlu pertimbangan alternatif pelarut karena kolagen dan kitosan lebih mudah larut dalam pelarut asam seperti asam klorida (HCl) dan asam asetat ( CH3COOH). Penelitian ini bertujuan untuk mengetahui Lethal Concentration 50 (LC50) dari pembalut luka kolagen-kitosan yang dilarutkan dalam pelarut DMSO, HCl, CH3COOH dengan metode Brine Shrimp Lethality Test (BSLT). Pembalut luka kolagen-kitosan didapatkan dengan mencampurkan larutan kitosan 2% dan kolagen dengan perbandingan 1:1 w/w kemudian dihomogenkan, dicetak, dan dikeringkan. Penelitian ini menggunakan uji sitotoksisitas dengan metode BSLT dan LC50 dihitung menggunakan Analisis Probit. Pembalut luka dilarutkan dalam pelarut DMSO 1%, CH3COOH 1%, dan HCl 1% hingga homogen, kemudian diencerkan dengan berbagai konsentrasi yaitu 100 ppm, 250 ppm, 250 ppm, 500 ppm, dan 1000 ppm dengan tiga kali ulangan untuk setiap perlakuan. Setelah itu uji BSLT dilakukan dengan menggunakan Artemia salina. Hasil penelitian menunjukkan bahwa pembalut luka yang dilarutkan dalam DMSO 1% memiliki LC50 > 1000 ppm, sedangkan pada pelarut CH3COOH dan pelarut HCl menunjukkan LC50< 30. Kesimpulan dari penelitian ini adalah pelarut DMSO bersifat non-toksik (LC50 > 1000 ppm), tetapi pelarut CH3COOH 1% dan HCl 1% bersifat sangat toksik (LC50 < 30 ppm) sebagai pelarut alternatif pembalut luka kolagen-kitosan pada uji BSLT.

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