scholarly journals A Cytotoxic Activity of Tahongai (Kleinhovia hospita Linn.) Leaves Extracts Using Brine Shrimp Lethality Test

2019 ◽  
Vol 4 (3) ◽  
pp. 60
Author(s):  
Indah Solihah ◽  
Herlina Herlina ◽  
Riana Sari Puspita Rasyid ◽  
Tri Suciati ◽  
Khairunnisa Khairunnisa
Keyword(s):  
Statistical Analysis ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Ethanol Extracts

Tahongai (Kleinhovia hospita Linn.) leaves had been known contain alkaloids, flavonoids, saponins, steroids, and tannins. At Komering, South Sumatera tribe, tahongai leaves had been known to treat tumor, cancer, polyps, acne, and dysmenorrhea. The study of cytotoxic activity of tahongai bark and stem were done. This study aims to determine the cytotoxic activity of tahongai leaves extracts using BSLT method. Tahongai leaves were extracted using gradual maceration with n-hexane, ethyl acetate, and ethanol 96%. Each extract was tested cytotoxic activity towards Artemia salina L. larvae. The yield of n-hexane, ethyl acetate, and ethanol extracts are 2,686%, 7,033%, and 7,933% respectively. Ethanol extract of tahongai leaves had the best cytotoxic activity with lethality value 76,667% at 500ppm. Statistical analysis with two way ANOVA showed extract and concentration had a significant (p<0,05) effect on larvae lethality percentage.

scholarly journals ANALISIS SENYAWA METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK ETANOL BATANG TANAMAN PATAH TULANG (Euphorbia tirucalli L.) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2014 ◽  
Vol 14 (2) ◽  
pp. 106 ◽  
Author(s):  
Grace S. Baud ◽  
Meiske S. Sangi ◽  
Harry S.J. Koleangan
Keyword(s):  
Secondary Metabolite ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Euphorbia Tirucalli ◽  
Ethanol Extracts

ABSTRAK Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman Patah tulang (Euphorbia tirucalli L.). Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia yaitu senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak segar dan kering batang tanaman Patah tulang. Penentuan toksisitas ekstrak etanol batang tanaman Patah tulang menggunakan metode Brine Shrimp Lethality Test (BSLT). Uji toksisitas digunakan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh positif flavonoid, tanin dan steroid sedangkan alkaloid, saponin dan triterpenoid negatif. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan metode probit menggunakan perangkat lunak SPSS 20.0 (untuk sistem operasi Windows). Hasil uji toksisitas ekstrak etanol batang Patah tulang menunjukkan bahwa ekstrak segar maupun kering bersifat sitotoksik dengan nilai LC50 (7,994ppm) untuk ekstrak etanol batang segar  dan LC50 (9,940ppm) untuk ekstrak etanol batang kering. Kata kunci : Euphorbia tirucalli L., Senyawa metabolit sekunder, Toksisitas, Artemia salina Leach, LC50.   ANALYSIS OF SECONDARY METABOLITE COMPOUNDS AND TOXICITY TEST OF  STEM  PLANT ETHANOL EXTRACTS OF  PATAH TULANG (Euphorbia tirucalli L.) BY BRINE SHRIMP LETHALITY TEST’S METHOD (BSLT) ABSTRACT A research on the analysis of secondary metabolite compounds contained in the stem of  patah tulang (Euphorbia tirucalli L.) and its toxicity had been done. The analysis was performed through phytochemical screening of alkaloids, flavonoids, triterpenoids, steroids, saponins and tannins on extracts of fresh and dried stem of the plant. Toxicity of ethanol extract of the stem was determined using Brine Shrimp Lethality Test (BSLT) method with Artemia salina Leach as bioindicator. Phytochemical screening showed positive results for flavonoids, tannins, and steroids and negative one for alkaloids, saponins, and triterpenoids. Toxicity data were obtained from Lethality Concentration 50 (LC50) calculation trhrough probit analysis using SPSS 20.0 software (for Windows operating systems). Toxicity test showed that ethanol extracts of the fresh and dried stem were cytotoxic with LC50 value of 7.994 ppm and 9.940 ppm, respectively. Keywords: Euphorbia tirucalli L., Secondary metabolite compounds, Toxicity, Artemia salina Leach, LC50.

scholarly journals Analisis Fitokimia Dan Toksisitas Dari Ekstrak Daun Tumbuhan Mempening (Lithocarpus bancanus (Scheff.) Rehd) Menggunakan Metode BSLT (Brine Shrimp Lethality Test)

2019 ◽  
Vol 2 (2) ◽  
pp. 29-33
Author(s):  
Retno Puji Lestari ◽  
Rudi Hendra Sy ◽  
Hilwan Yuda Teruna
Keyword(s):  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
The Family ◽  
Tribal Region

Mempening (Lithocarpus bancanus (Scheff.) Rehd) is a species belongs to the family Fagaceae which is found growing around the Talang Mamak tribal region in Kelayang District, Indragiri Hulu Regency, Riau Province. The purpose of this study was to determine the toxicity of leaf extract of L. bancanus (Scheff.) Rehd against Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method and its phytochemical analysis. The results of the toxicity was determined against n-hexane, dichloromethane, ethyl acetate, methanol, and ethanol extract and they showed LC50 values ​​of 3.15 ppm; 41163.26 ppm; 5817.09 ppm; 817, 27 ppm; 9019.70 ppm. Phytochemical analysis of the leaves of L. bancanus (Scheff.) Rehd plant showed a class of flavonoids, terpenoids, phenolics and saponins.

scholarly journals KAJIAN TOKSISITAS DARI TUBUH BUAH Ganoderma lucidum DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST)

2014 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
Ross Nurul Rohmah ◽  
Nuniek Ina Ratnaningtyas ◽  
Ari Asnani
Keyword(s):  
Toxic Effect ◽  
Fruiting Body ◽  
Brine Shrimp ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
One Stage ◽  
Body Extract

Ganoderma lucidum is polyporus fungi from Basidiomycetes which can be used as traditional medicines. Toxicity test with Brine Shrimp Lethality Test (BST) method using Artemia salina was conducted to find out toxic effect of G.lucidum. An extract would have toxic effect if the LC50 < 1000 µg/ml. The aims of this experiment were to know about the toxicity level from G.lucidum with Brine Shrimp Lethality Test (BST) method and determined the concentration of fruiting body extract of G.lucidum which had the best toxicity effect in LC50. There were two extraction methods used in this experiment, first one stage extraction and then multilevel extraction with hexane, ethyl acetate, and ethanol. Each solvent was made in 1000 ppm, 500 ppm, 250 ppm, and 125 ppm. This process was repeated for three times. This experiment used G.lucidum extract which was tested to A.salina and secunder compound metabolit test from the most toxic G. lucidum was done with phytochemical analysis. The result indicated that fruiting body extract from G.lucidum could kill A.salina because all extract was positively contained alkaloid and terpenoid but negatively contained flavonoid. Fruiting body extract from G.lucidum which had lowest LC50 wass ethyl asetat extract with one stage extraction in concentration 53,70 ppm and highest LC50 was ethanol extract with multilevel extraction in concentration 501,18 ppm.

scholarly journals UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2019 ◽  
Vol 7 (3) ◽  
pp. 265
Author(s):  
Faradila Y. Karim ◽  
Nickson J Kawung ◽  
Billy Th. Wagey
Keyword(s):  
Cytotoxic Activity ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Bioactive Compound ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Thalassia Hemprichii ◽  
Brine Shrimp Lethality ◽  
A Value ◽  
Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)  

scholarly journals SKRINING FITOKIMIA, UJI AKTIVITAS ANTIOKSIDAN, DAN TOKSISITAS DARI EKSTRAK DAUN KERSEN (Muntingia calabura L.) DENGAN METODE 1.1-diphenyl-2-picrylhydrazyl (DPPH) dan Brine Shrimp Lethality Test (BSLT)

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 315
Author(s):  
Selin Widjaya ◽  
Widdhi Bodhi ◽  
Adithya Yudistira
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Brine Shrimp Lethality ◽  
Phytochemical Content ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Dpph Method ◽  
Muntingia Calabura

ABSTRACTKersen (Muntingia calabura L.) is a plant that has begun to be eliminated and was rarely used because it is often considered to have no economic value and lack of knowledge about its utilization, whereas kersen plants contain flavonoids, saponins, and tannins which were have high benefit for health. The content of metabolites is affected by soil nutrient elements and difference place of growth. This study aims to determine the potential of kersen leaves grown in North Minahasa based on phytochemical content, ability of antioxidant activity, and toxicity. Kersen leaves were extracted using sequential maceration method with n-hexane, ethyl acetate, and ethanol as solvents. Phytochemical Screening using several reagents which tailored to the type of phytochemical test. 1.1-diphenyl-2-picrylhydrazyl (DPPH) method is used to evaluate antioxidant activity, and Brine Shrimp Lethality Test (BSLT) method is used to evaluate toxicity. The result of this study indicate that the n-hexane extract contains phenols, flavonoids, and tannins, with IC50 value 12.54 μg/mL, and LC50 value 881 μg/mL. Ethyl acetate extract contains phenols, flavonoids, tannins, and saponins, with IC50 value 61.3 μg/mL, and LC50 value 1758 μg/mL. Ethanol extract has phenol, flavonoid, tannin, saponin, and terpenoid content, with IC50 value 9.01 μg/mL, and LC50 value 106 μg/mL. Keywords : Kersen leaves, Antioxidant, Toxicity, IC50, LC50 ABSTRAKKersen (Muntingia calabura L.) merupakan tanaman yang sudah mulai tersingkirkan dan jarang dimanfaatkan karena sering dianggap tidak punya nilai ekonomis dan kurangnya pengetahuan tentang pemanfaatannya, padahal tanaman kersen memiliki kandungan flavonoid, saponin, dan tanin yang bermanfaat tinggi untuk kesehatan. Kandungan senyawa metabolit dipengaruhi oleh unsur hara tanah dan perbedaan tempat tumbuh. Penelitian ini bertujuan untuk mengetahui potensi dari daun kersen yang tumbuh di Minahasa Utara berdasarkan kandungan fitokimia, kemampuan aktivitas antioksidan, dan toksisitasnya. Ekstrak daun kersen diekstraksi dengan metode maserasi sekuensial menggunakan pelarut n-heksan, etil asetat, dan etanol.  Skrining fitokimia menggunakan beberapa reagen yang disesuaikan dengan jenis uji fitokimia. Metode 1.1-diphenyl-2-picrylhydrazyl (DPPH) digunakan untuk mengevaluasi aktivitas antioksidan, dan metode Brine Shrimp Lethality Test (BSLT) digunakan untuk mengevaluasi toksisitas. Hasil penelitian ini menunjukkan bahwa ekstrak n-heksan memiliki kandungan fenol, flavonoid, dan tanin, nilai IC50 12,54 μg/mL, dan nilai LC50 881 μg/mL. Ekstrak etil asetat memiliki kandungan fenol, flavonoid, tanin, dan saponin, nilai IC50 61,3 μg/mL, dan nilai LC50 1758 μg/mL. Ekstrak etanol memiliki kandungan fenol, flavonoid, tanin, saponin, dan terpenoid, nilai IC50 9,01 μg/mL, dan nilai LC50 106 μg/mL. Kata kunci : Daun Kersen, Antioksidan, Toksisitas, IC50, LC50

scholarly journals Toxicity Test of Ethanol Extract Ant Plant Local Aceh (Mymercodia sp) Method of BSLTLarvae Shrimp Artemia salina Leach

2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Frengki F ◽  
Roslizawaty R ◽  
Desi Pertiwi
Keyword(s):  
Crude Extract ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Rotary Evaporator

Toxicity test and phytochemical screening of ant plant ethanol extract were done. The toxicity test was done by employing the Brine Shrimp Lethality Test (BSLT) method. Sample of ant plant taken from the Aceh Besar forest have been extracted with ethanol. The sample was thenconcentrated using a rotary evaporator until a crude extract was obtained. The extract was tested by toxicity to larva Artemia salina L. Thetoxicity test for larvae Artemia salina Leach have been done by employing the Brine Shrimp Lethality Test (BSLT) method at four concentrationlevels, i.e: 250 ppm, 125 ppm, 50 ppm, and 25 ppm. The result of the research showed that ethanol extract of ant plant Aceh had acute toxicityby LC value 61.11 ppm. The extract had identified were fenolik, saponin, steroid and terpenoid.____________________________________________________________________________________________________________________Key words: rine Shrimp Lethality Test, LC5050, Ant Plant, Aceh, Bphytochemical Screening 

scholarly journals Uji Toksisitas Akut Ekstrak dan Fraksi Kulit Batang Ketapang Laut (Terminalia Catappa L.) Menggunakan Metode BSLT

2020 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Ari Sartinah ◽  
Yamin Yamin ◽  
Muhammad Arba ◽  
Nur Illiyyin Akib ◽  
Andi Nafisah Tendri Adjeng ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Program Analysis ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Brine Shrimp Lethality ◽  
Terminalia Catappa ◽  
Water Fractions

AbstrakToksisitas akut merupakan kemampuan suatu bahan kimia (obat/bahan obat) dalam menimbulkan kerusakan  pada suatu organisme dalam waktu yang relatif singkat. Tujuan penelitian ini adalah untuk mengetahui potensi ketoksikan akut dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L.). Pengujian toksisitas akut dilakukan dengan menggunakan metode BSLT (Brine Shrimp Lethality Test) dan menggunakan analisis program untuk mengetahui nilai LC50 dari ekstrak dan fraksi. Hasil uji toksisitas akut menunjukan nilai LC50  dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L. )  berturut-turut 247,997 ppm; 400,666 ppm dan 618,046 ppm. Hal ini menunjukan bahwa ekstrak metanol batang ketapang laut (Terminalia catappa L.) bersifat paling toksik dibandingkan fraksi etil asetat dan fraksi air.Kata kunci: Ekstrak, Fraksi, Terminalia catappa L., BSLT, Artemia salina LeachAbstractAcute toxicity is the ability of chemical (drug/drug ingredient) to cause damage to organisms in a relatively short time. The purpose of this study was to determine the potential for acute toxicity of methanol extract, ethyl acetate and water fractions of sea ketapang stem skin (Terminalia catappa L.). Acute toxicity testing was performed using the BSLT (Brine Shrimp Lethality Test) method and program analysis to determine the LC50 values of extracts and fractions. The results of the acute toxicity test showed the LC50 values of methanol extract, ethyl acetate and water fractions of the plant stem skin consecutively were 247,997 ppm; 400,666 ppm and 618,046 ppm. This shows that the methanol extract of sea ketapang stems (Terminalia catappa L.) is the most toxic compared to the ethyl acetate and the water fraction.Keywords: Extract, Fraction, Terminalia catappa L., BSLT, Artemia salina Leach

scholarly journals UJI TOKSISITAS EKSTRAK ETANOL DAUN JAMBU BIJI AUSTRALIA (Psidium guajava L) DENGAN METODE BSLT (Brine Shrimp Lethality Test)

2021 ◽  
Vol 9 (1) ◽  
pp. 10-17
Author(s):  
Nofita Nofita ◽  
◽  
Ade Maria Ulfa ◽  
Miera Delima ◽  
◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Ultrasonic Method ◽  
Ethanol Extract ◽  
Psidium Guajava ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Test Method ◽  
Toxicity Tests ◽  
Brine Shrimp Lethality ◽  
Guava Leaves

ABSTRACTGuava is one of the plants that can traditionally be used for the treatment of diseases. Many kinds of guava, one of which is the Australian guava has the characteristics of roots, stems, leaves, dark red fruit. This study aims to determine the toxicity of the ethanol extract of Australian guava leaves (Psidium guajava L) using the BSLT (Brine Shrimp Lethality Test) method and determine the chemical content of Australian guava leaves (Psidium guajava L). The extract was made by the ultrasonic method using 96% ethanol solvent. Toxicity tests were carried out using 48-hour-old Artemia salina Leach shrimp larvae. The toxic effect of the extract was identified by the percentage of shrimp larvae mortality using probit analysis (LC50). From the research results, phytochemical content includes tannins, flavonoids, alkaloids, terpenoids and saponins, and flavonoid compounds have the highest content compared to the others. Research shows that the ethanol extract of Australian guava leaves is of a moderate category (LC50 441,977 ppm).Keywords :Australia guava leaves, BSLT, Artemia salina L, Ultrasonic

scholarly journals Cytotoxic Activity of Orsellinates

2006 ◽  
Vol 61 (9-10) ◽  
pp. 653-657 ◽  
Author(s):  
Alcir T. Gomes ◽  
Neli K. Honda ◽  
Fernanda M. Roese ◽  
Rozanna M. Muzzi ◽  
Leandro Sauer
Keyword(s):  
Cytotoxic Activity ◽  
Natural Product ◽  
Hydroxy Group ◽  
Brine Shrimp ◽  
Linear Chain ◽  
Artemia Salina ◽  
Chain Elongation ◽  
Log P ◽  
Brine Shrimp Lethality ◽  
Parmotrema Tinctorum

AbstractThe series of 2,4-dihydroxy-6-methylbenzoates 2-10 (methyl to hexyl orsellinates) prepared by alcoholysis of lecanoric acid (1) - a natural product from the lichen Parmotrema tinctorum (Nyl.) Hale - was submitted to the brine shrimp lethality test (BST), which was also performed for 2,4-dihydroxy-6-methylbenzoic acid (11) (orsellinic acid) and the derivative ethyl-2-hydroxy-4-methoxy-6-methylbenzoate (12) (4-methoxy-ethyl orsellinate), in order to detect new substances with probable antineoplasic activity. Results showed that chain elongation - increase in lipophilicity (log P) - causes a rise in the cytotoxic activity of orsellinates. Hexyl orsellinate (7) showed the highest cytotoxic activity (LC50 = 31 μм). A correlation between lipophilicity (log P) and cytotoxic activity (log 1/LC50) is presented. Compounds with ramified chains - iso-propyl, sec-butyl and tert-butyl orsellinates (8-10) - were less active than those with the correspondent linear chain. The activities presented by 4-methoxy-ethyl orsellinate (12) and ethyl orsellinate (3) suggest that the hydroxy group at the C-4 position causes effect in the cytotoxic activity of orsellinates against Artemia salina.

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