scholarly journals UJI AKTIVITAS SITOTOKSIK EKSTRAK METANOL BUAH KETUMBAR (Coriandrum sativum Linn) TERHADAP ARTEMIA SALINA LEACH DENGAN UJI BSLT (Brine Shrimp Lethality Test)

2020 ◽  
Vol 2 (3) ◽  
pp. 137-146
Author(s):  
Mega Yulia ◽  
Rani Anggraini ◽  
Farizal Farizal
Keyword(s):  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Coriandrum Sativum ◽  
Brine Shrimp Lethality ◽  
Activity Test ◽  
Anti Cancer ◽  
Expected Benefits

Coriander is a common herb can be used as traditional medicine. One of the expected benefits of Coriander fruits is an anti-cancer. This study aims to determine the cytotoxic activity of Coriander extract (Coriandrum sativum Linn) on shrimp larvae (Artemia salina Leach) with the BSLT (Brine Shrimp Lethality Test) method. Coriander fruits was extracted using methanol solvent and maceration method, then concentrated it with a rotary evaporator until a thick extract. Cytotoxic activity test used methanol extract with several concentration : 1,000 ppm, 100 ppm, 10 ppm, and 1 ppm. The result of this research reveal that methanol extract of Coriander fruits has cytotoxic activity with LC50 value is 32.35 ppm.

scholarly journals Uji Toksisitas Akut Ekstrak dan Fraksi Kulit Batang Ketapang Laut (Terminalia Catappa L.) Menggunakan Metode BSLT

2020 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Ari Sartinah ◽  
Yamin Yamin ◽  
Muhammad Arba ◽  
Nur Illiyyin Akib ◽  
Andi Nafisah Tendri Adjeng ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Program Analysis ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Brine Shrimp Lethality ◽  
Terminalia Catappa ◽  
Water Fractions

AbstrakToksisitas akut merupakan kemampuan suatu bahan kimia (obat/bahan obat) dalam menimbulkan kerusakan  pada suatu organisme dalam waktu yang relatif singkat. Tujuan penelitian ini adalah untuk mengetahui potensi ketoksikan akut dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L.). Pengujian toksisitas akut dilakukan dengan menggunakan metode BSLT (Brine Shrimp Lethality Test) dan menggunakan analisis program untuk mengetahui nilai LC50 dari ekstrak dan fraksi. Hasil uji toksisitas akut menunjukan nilai LC50  dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L. )  berturut-turut 247,997 ppm; 400,666 ppm dan 618,046 ppm. Hal ini menunjukan bahwa ekstrak metanol batang ketapang laut (Terminalia catappa L.) bersifat paling toksik dibandingkan fraksi etil asetat dan fraksi air.Kata kunci: Ekstrak, Fraksi, Terminalia catappa L., BSLT, Artemia salina LeachAbstractAcute toxicity is the ability of chemical (drug/drug ingredient) to cause damage to organisms in a relatively short time. The purpose of this study was to determine the potential for acute toxicity of methanol extract, ethyl acetate and water fractions of sea ketapang stem skin (Terminalia catappa L.). Acute toxicity testing was performed using the BSLT (Brine Shrimp Lethality Test) method and program analysis to determine the LC50 values of extracts and fractions. The results of the acute toxicity test showed the LC50 values of methanol extract, ethyl acetate and water fractions of the plant stem skin consecutively were 247,997 ppm; 400,666 ppm and 618,046 ppm. This shows that the methanol extract of sea ketapang stems (Terminalia catappa L.) is the most toxic compared to the ethyl acetate and the water fraction.Keywords: Extract, Fraction, Terminalia catappa L., BSLT, Artemia salina Leach

Toksisitas Akut Ektrak N-Heksan Dan Methanol Rimpang Jeringau Merah Terhadap Kematian Artemia Salina Leach Dengan Metode Brine Shrimp Lethality Test

2018 ◽  
Vol 1 (2) ◽  
pp. 125
Author(s):  
Edy Suwandi ◽  
Supriyanto Supriyanto
Keyword(s):  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Test Method ◽  
Endemic Plant ◽  
Brine Shrimp Lethality ◽  
Purposive Sampling ◽  
Study Results ◽  
Sweet Flag

Abstract: Acorus sp (red sweet flag) is an endemic plant from West Borneo which contains chemical substances such as flavonoids that have bioactivity as antioxidants so it is recommended one to prevent cancer. The study aimed to determine toxicity of n-Hexan extract and methanol red sweet flag rhizomes against Artemia salina Leach with Brine Shrimp Lethality Test method. This study was a type of quasi experimental design, sampling technique obtained by using purposive sampling. According to the study results, it can be seen than methanol extract from red sweet flag rhizomes give impact on the mortality average of Artemia salina Leach : concentration 100 µg/ml (51%), 250 µg/ml (75%), 500 µg/ml (92%), 750 µg/ml (96%) and 1000 µg/ml (99%). In addition, n-Hexan extract of red sweet flag rhizomes in 100 µg/ml (45%), 250 µg/ml (60%), 500 µg/ml (92%), 750 µg/ml (84%) and 1000 µg/ml (96%). It is reported based on probit analysis that LC50 of n-Hexan extract from red sweet flag rhizomes was 128.2921 µg/ml meanwhile methanol extract was 242.7169 µg/ml µg/ ml, thus in conclusion n-Hexan and methanol extract of red sweet flag rhizomes potentially cytotoxic to Artemia salina Leach larvae.Abstrak: Jeringau merah (Acorus sp) merupakan satu jenis tanaman endemik Kalimantan Barat memiliki kandungan kimia diantaranya flavonoidyang mempunyai bioaktivitas sebagai antioksidan sehingga sangat baik untuk pencegahan kanker. Penelitian ini bertujuan mengetahui mengetahui dan menentukan toksisitas ekstrak n-Heksan dan Methanol Rimpang Jeringau MerahterhadapArtemia salinaLeachdengan metode Brine Shrimp Lethality Test. Desain penelitian ini adalah eksperimental semu, teknik pengambilan sampel dengan cara purposive sampling. Berdasarkan hasil penelitian diketahui ekstrak Methanol Rimpang Jeringau Merah rerata kematianArtemia salina Leach konsentrasi 100 µg/ml (51%), 250 µg/ ml (75%), 500 µg/ml (92%), 750 µg/ml (96%) dan 1000 µg/ml (99%)pada ekstrak n-Heksan Rimpang Jeringau Merah konsentrasi 100 µg/ml (45%), 250 µg/ml (60%), 500 µg/ml (92%), 750 µg/ml (84%) dan 1000 µg/ml (96%). Dari analisis probit diketahuinilai LC50 ekstrak n-Heksan Rimpang Jeringau Merah yaitu 128.2921 µg/ml dan ekstrak Methanol Rimpang Jeringau Merah yaitu 242.7169 µg/ml µg/ml, maka disimpulkan ekstrak n-Heksan dan Methanol Rimpang Jeringau Merahbersifat sitotoksik terhadap larva Artemia salina Leach.

scholarly journals ANALISIS KANDUNGAN FITOKIMIA DARI UJI TOKSISITAS EKSTRAK METANOL DAUN SOYOGIK (Sauraula bracteosa DC) DENGAN MENGGUNAKAN METODE MASERASI

2013 ◽  
Vol 13 (2) ◽  
pp. 98 ◽  
Author(s):  
Morein A Maukar ◽  
Max R.J. Runtuwene ◽  
Julius Pontoh
Keyword(s):  
Brine Shrimp ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Test Method ◽  
Tannin Content ◽  
Toxicity Assay ◽  
Brine Shrimp Lethality ◽  
Antioxidants Activity

ANALISIS KANDUNGAN FITOKIMIA DARI UJI TOKSISITAS EKSTRAK METANOL  DAUN SOYOGIK (Sauraula bracteosa DC) DENGAN MENGGUNAKAN  METODE MASERASI ABSTRAK Penelitian ini bertujuan untuk mengetahui LC50 ekstrak metanol tanaman soyogik yang diekstraksi secara maserasi dan menganalisis kandungan fenolik. Analisis kandungan fitokimia meliputi uji fenolik, uji flavonoid dan uji tanin . Uji toksisitas menggunakan metode BSLT (Brine Shrimp Lethality Test) dengan menggunakan larva Artemia Salina Leach. Hasil yang diperoleh dan dianalisis  dengan analisis probit menggunakan S.PSS 20.0 untuk  mengetahui  nilai (LC50). Nilai LC50 yang diperoleh berdasarkan uji toksisitas daun soyogik yaitu sebesar 37,30  ppm. Kandungan fenolik, flavonoid  dan tanin berturut-turut adalah 43,06, 6,52 dan 17,91. Kata Kunci : Daun soyogik, Fitokimia, Toksisitas, Artemia salin FITOCHEMISTRY CONTENT ANALYSIS OF TOXICITY TEST OF METHANOL EXTRACT OF SOYOGIK LEAVE (Sauraula bracteosa DC) USING MASERATION METHOD   ABSTRACT The aims of this research are to measure the Lethal Concentration 50 (LC50) in toxicity activity  methanol extracts from soyogik plants that were extracted by maceration and to analyzing its phenolic content. Phytochemical screening include phenolic, flavonoid and tannin test.  Toxicity assay using BSLT (Brine Shrimp Lethality Test) method using Artemia Salina Leach. Results were analyzed by probit analysis using SPSS 20.0 for Windows to know the value of (LC50). Screening phytochemicals shows that there is antioxidants activity of Soyogik leaves. The LC50 values ​​were obtained by testing the toxicity of Soyogik leaves is equal to 37.301 ppm. The phenolic, flavonoid and tannin content were 43,06, 6,52 and 17,91, respectively. Keywords : Soyogik leaves, Phytochemical, Toxicity, Artemia salina.

Identification of Secondary Metabolite Compounds and Cytotoxic Activity Test of Papuan Grape (Sararanga Sinuosa Hemsley) Extract using Brine Shrimp Lethality Test (BSLT) Method

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Elsye Gunawan ◽  
Enrick Kharo Etmond ◽  
Linus Yhani Chrystomo
Keyword(s):  
Cytotoxic Activity ◽  
Secondary Metabolite ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Organ System ◽  
Activity Test ◽  
Anti Cancer ◽  
Extraction Stage ◽  
Cancer Agent ◽  
Concentration Series

Papua has a diversity of flora species, one of which is the Papuan Grape (Sararanga sinuosa Hemsley). It is commonly used by the Depapre community, Jayapura, as a stamina booster. This research aims to identify the secondary metabolite compounds, to test the cytotoxic activity of Papuan Grape (Sararanga sinuosa Hemsley) extract, and to determine the best concentration that inhabits the growth of Artemia salina larvae using the BSLT method. This study was conducted with the extraction stage using the maceration method by making use of 96% ethanol solvent. Subsequently, the concentration series 0, 50, 100, 150, 200, 250, 300 ppm of Papuan Grape (Sararanga sinuosa Hemsley) extract were made to test the cytotoxic activity on the mortality of Artemia salina shrimp larvae. The results showed that Alkaloids, Flavonoids, Saponins, and Tannins were compounded as secondary metabolite. An antioxidant research that had been carried out previously had LC50 of green-white fruit (12,49 ± 0,35 mg/ml), orange-red fruit (17,62 ± 3,49 mg/ml) and red fruit (12,23 ± 0,46 mg/ml). The community process one stalk of it into juice and used or consumed it two times a day. An inappropriate dose of traditional medicine usage can affect the organ system and had adverse effects in the future The result of cytotoxic research obtained the value of LC50 in ethanol extract of Papuan Grape was 140,863 ppm, and concentration of 250 ppm was the best concentration to inhibit the growth of shrimp larvae (Artemia salina L). The conclusion of this study was the ethanol extract of Papuan Grape (Sararanga sinuosa Hemsley) showed the highest cytotoxic activity and potentially become an anti-cancer agent.

scholarly journals UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2019 ◽  
Vol 7 (3) ◽  
pp. 265
Author(s):  
Faradila Y. Karim ◽  
Nickson J Kawung ◽  
Billy Th. Wagey
Keyword(s):  
Cytotoxic Activity ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Bioactive Compound ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Thalassia Hemprichii ◽  
Brine Shrimp Lethality ◽  
A Value ◽  
Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)  

scholarly journals Steroids from the Leave of Persicaria perfoliata (L.) H. Gross

2019 ◽  
Vol 22 (2) ◽  
pp. 224-227
Author(s):  
Md Sagar Hossain ◽  
NM Mofiz Uddin Khan ◽  
Md Saiful Quddus ◽  
AM Sarwaruddin Chowdhury
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
Antimicrobial Screening ◽  
Brine Shrimp Lethality ◽  
Persicaria Perfoliata ◽  
Test Organisms

β-sitosterol (1) and β-sitosterol-D-glucoside (2) were isolated from the dichloromethane soluble fraction of a methanol extract of the leave of Persicaria perfoliata. The crude fraction was subjected to antimicrobial screening and brine shrimp lethality bioassay, where moderate antimicrobial activity was observed against most of the test organisms was seen. The hexane soluble fraction also displayed significant cytotoxic activity with LC50 0.64 μg/ml in brine shrimp lethality bioassay. This is the first report of occurrence of these compounds from this plant. Bangladesh Pharmaceutical Journal 22(2): 224-227, 2019

scholarly journals Antioxidant, Antibacterial and Cytotoxic Activity of the Methanol Extract of Urtica Crenulata

2009 ◽  
Vol 2 (1) ◽  
pp. 169-177
Author(s):  
M. A. Rahman ◽  
M. S. Rana ◽  
M. M. Zaman ◽  
S. A. Uddin ◽  
R. Akter
Keyword(s):  
Ascorbic Acid ◽  
Free Radical ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Brine Shrimp Lethality ◽  
Brine Shrimp Lethality Assay ◽  
Stem Extract

The antioxidant, antibacterial and cytotoxic properties of methanol extract of Urtica crenulata (syn: Laportea crenulata Gaud) stem has been investigated in the present study. The antioxidant and cytotoxic activity of the Urtica crenulata methanol extract were assessed by DPPH free radical scavenging method and brine shrimp lethality bioassay method, respectively. The antibacterial activity of the plant extract (500 μg/disc) was also carried out by disc diffusion technique. Stem extract showed DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging effect compared with ascorbic acid. IC50 value of ascorbic acid and stem extract was found 14.72 μg/ml and 1468.9 μg/ml, respectively. In antibacterial experiment, Urtica crenulata stem extract showed 8, 14 and 10 mm of diameter of zone inhibition against Salmonella typhi, Shigella flexneri and Shigella sonnei, respectively and 9 and 8 mm of diameter of zone inhibition against Bacillus subtilis and Bacillus cereus but no activity was observed against Staphylococcus aureus. In brine shrimp lethality assay, the LC50 value of the extract was found 104.0 μg/ml, which indicates that the extract has high cytoxic effect. The present study demonstrates that methanol extract of Urtica crenulata stem has significant cytotoxic effect. The extract also showed some moderate antibacterial and minimum significant antioxidant effects.  Keywords: Urtica crenulata;  Antioxidant; Antibacterial; Cytotoxic; BHT. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i1.2872             J. Sci. Res. 2 (1), 169-177 (2010) 

scholarly journals Uji Sitotoksik Ekstrak Etanol Buah Ketumbar (Coriandrum Sativum Linn) Terhadap Artemia Salina Leach Dengan Metode Brine Shrimp Lethality Test (BSLT)

2017 ◽  
Vol 2 (2) ◽  
pp. 86
Author(s):  
Febri Tianandari ◽  
Rasidah Rasidah
Keyword(s):  
Brine Shrimp ◽  
Artemia Salina ◽  
Coriandrum Sativum ◽  
Brine Shrimp Lethality

Ketumbar (Coriandrum sativum L.) adalah salah satu jenis tanaman rempah-rempah yang sudah sangat dikenal di masyarakat. Skrining fitokimia pendahuluan serbuk ketumbar mengungkapkan adanya kandungan protein, karbohidrat, senyawa fenolik, tanin, flavonoid. Senyawa sitotoksik adalah suatu senyawa atau zat yang dapat merusak sel normal dan sel kanker dan juga dapat digunakan untuk menghambat pertumbuhan sel tumor. Penelitian ini dilakukan untuk mengetahui efek sitotoksik ketumbar terhadap Arthemia salina Leach, dengan metode Brine Shrimp Lethality Test (BSLT). Hasil penelitian ini diketahui bahwa ekstrak etanol 70% buah ketumbar (Coriandrum sativum Linn) memiliki efek sitotoksik terhadap larva Arthemia salina Leach. Nilai LC50 dari ekstrak etanol buah ketumbar (Coriandrum sativum Linn)  terhadap larva Arthemia salina Leach yaitu 40,548 ppm.Kata kunci: Sitotoksik, ketumbar (Coriandrum sativum Linn), BSLT 

scholarly journals Skrining Fitokimia dan Uji Aktivitas Sitotoksik dari Tumbuhan Sarang Semut (Myrmecodia beccarii Hook.f.) Asal Kabupaten Merauke

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Modustriati P. Mardany ◽  
Linus Y. Chrystomo ◽  
Aditya K. Karim
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Medicinal Plant ◽  
Cytotoxic Activity ◽  
Active Compound ◽  
Brine Shrimp ◽  
Ethanolic Extract ◽  
Artemia Salina ◽  
Test Method ◽  
High Cytotoxic Activity

Sarang semut (Myrmecodia beccarii Hook.f.) is a famous medicinal plant of Papua, which has traditionally potential in treating a wide variety of mild and severe illnesses, such as cancers and tumors, gout, coronary heart disease, hemorrhoids, tuberculosis, migraines, rheumatism, and leukemia. The purposes of this study were to determine the content of the active compound group and the cytotoxic activity of sarang semut plants from Merauke Region. The extraction was done by maceration using 96 % ethanol. The concentration of ethanolic extract were 0, 25, 50, 75, 100 ppm. The results of the study showed that the cytotoxic activity determine using BSLT (Brine Shrimp Lethality Test) method have a high cytotoxic activity with LC50 values of 22.86 ppm and containing the active compound namely flavonoids, tannins and saponins separately. The ethanolic extract of tuber of sarang semut (M. beccarii) has a high cytotoxic activity against larvae of Artemia salina Leach according BSLT method. Key words: Active compound, M. beccarii, cytotoxic activity, Merauke.

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