Pengaruh Penggunaan Jenis Pelarut dalam Uji Sitotoksistas Metode Brine Shrimp Lethality Test (BSLT) pada Wound Dressing Kolagen-Kitosan

al-Kimiya ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 15-20
Author(s):  
Ary Andini ◽  
Endah Prayekti ◽  
Fadillah Triasmoro ◽  
Indah Nur Kamaliyah
Keyword(s):  
Brine Shrimp ◽  
Wound Dressing ◽  
Artemia Salina ◽  
Lethal Concentration ◽  
Brine Shrimp Lethality

Kolagen dan kitosan dapat digunakan sebagai bahan pembalut luka karena memiliki karakteristik yang baik. Namun, pembalut luka kolagen-kitosan perlu dilakukan uji sitotoksisitas sebelum diaplikasikan secara in vivo, seperti Brine Shrimp Lethally Test (BSLT). Pembalut luka kolagen-kitosan tidak dapat larut dalam Dimetil Sulfoksida (DMSO) dan aquadest dengan mudah, oleh karena itu perlu pertimbangan alternatif pelarut karena kolagen dan kitosan lebih mudah larut dalam pelarut asam seperti asam klorida (HCl) dan asam asetat ( CH3COOH). Penelitian ini bertujuan untuk mengetahui Lethal Concentration  50 (LC50) dari pembalut luka kolagen-kitosan yang dilarutkan dalam pelarut DMSO, HCl, CH3COOH dengan metode Brine Shrimp Lethality Test (BSLT). Pembalut luka kolagen-kitosan didapatkan dengan mencampurkan larutan kitosan 2% dan kolagen dengan perbandingan 1:1 w/w kemudian dihomogenkan, dicetak, dan dikeringkan.  Penelitian ini menggunakan uji sitotoksisitas dengan metode BSLT dan LC50 dihitung menggunakan Analisis Probit. Pembalut luka dilarutkan dalam pelarut DMSO 1%, CH3COOH 1%, dan HCl 1% hingga homogen, kemudian diencerkan dengan berbagai konsentrasi yaitu 100 ppm, 250 ppm, 250 ppm, 500 ppm, dan 1000 ppm dengan tiga kali ulangan untuk setiap perlakuan. Setelah itu uji BSLT dilakukan dengan menggunakan Artemia salina. Hasil penelitian menunjukkan bahwa pembalut luka yang dilarutkan dalam DMSO 1% memiliki LC50 > 1000 ppm, sedangkan pada pelarut CH3COOH dan pelarut  HCl menunjukkan  LC50< 30. Kesimpulan dari penelitian ini adalah pelarut DMSO bersifat non-toksik (LC50  > 1000 ppm), tetapi pelarut CH3COOH 1% dan HCl 1% bersifat sangat toksik (LC50 < 30 ppm) sebagai pelarut alternatif pembalut luka kolagen-kitosan pada uji BSLT.

scholarly journals Uji Aktivitas Toksisitas Dan Antimikroba Flavonoid Total Daun Benalu (Dendrophthoe pentandra (L) Miq) Dari Pohon Glodokan (Polyalthia longifolia)

2018 ◽  
Vol 3 (1) ◽  
pp. 32-43
Author(s):  
ALIYAH FAHMI ◽  
Rumondang Bulan
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Lethal Concentration ◽  
Brine Shrimp Lethality ◽  
E Coli ◽  
Polyalthia Longifolia

Penelitian mengenai uji aktivitas toksisitas dan antimikroba flavonoid total daun benalu (Dendrophthoe pentandra (L) Miq) dari pohon glodokan (Polyalthia longifolia) telah dilakukan. Penelitian ini bertujuan untuk mengetahui aktivitas toksisitas dari flavonoid total daun benalu pohon glodokan berdasarkan metode Brine Shrimp Lethality Test (BSLT)  menggunakan larva udang Artemia salina Leach diperoleh Lethal Concentration (LC50) sebesar 30.06 mg/L yang berarti memiliki aktivitas toksisitas yang toksik sementara untuk aktivitas antimikroba dari flavonoid total daun benalu pohon glodokan dengan Metode Difusi Agar diperoleh diameter zona hambat pada Streptococcus  mutans pada konsentrasi sampel flavonoid total pohon glodokan 3%, 6% dan 9 % adalah 6; 9 dan 17.25 mm, pada Escherichia coli adalah 3.55; 4.25 dan 9.15 mm dan pada Candida albicans adalah 8.30; 4 dan 5.30 mm dimana semakin besar konsentrasi maka daya hambat pada S. mutans dan E. coli semakin besar namun pada C. albicans kurang mempengaruhi tetapi memiliki aktivitas hambat yang baik sehingga efektif dikembangkan sebagai zat antimikroba.

scholarly journals Toksisitas kombinasi ekstrak etanol 70% daun petai cina (Leucaena leucocephala (Lam.) de Wit) dan kulit jengkol (Archidendron jiringa (Jack) I.C.Nielsen) dengan metode Brine Shrimp Lethality Test

2019 ◽  
Vol 8 (1) ◽  
pp. 9
Author(s):  
Harry Noviardi ◽  
Sitaresmi Yuningtyas ◽  
Diah Ajeng Tri R ◽  
Ahmad Ben ◽  
Padmono Citroreksoko
Keyword(s):  
Leucaena Leucocephala ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Lethal Concentration ◽  
Brine Shrimp Lethality

Latar Belakang: Daun Petai cina dan kulit jengkol merupakan tumbuhan suku polong-polongan yang mengandung senyawa bahan alam seperti alkaloid, flavonoid, saponin, tanin dan triterpenoid. Senyawa bahan alam tersebut diduga berpotensi sebagai antikanker.Uji toksisitas digunakan untuk mendeteksi potensi senyawa antikanker.Tujuan penelitian ini adalah untuk menentukan nilai toksisitas dari kombinasi ekstrak daun petai cina dan kulit jengkol. Metode:Proses ekstraksi menggunakan daun petai cina dan kulit jengkol yang digunakan secara berturut-turut 1:1, 1:3, 1:5, 1:7, dan 1:9. Metode toksisitas yang digunakan adalah Brine Shrimp Lethality Test (BSLT)dengan hewan uji Artemia salina L. Parameter yang diukur adalah nilai lethal concentration (LC50). Hasil:Nilai LC50 yang diperoleh dari kombinasi ekstrak daun petai cina dan kulit jengkol 1:1, 1:3, 1:5, 1:7 dan 1:9 secara berturut-turut sebesar 85.27, 30.41, 21.76, 14.06 & 1.358 ppm. Hasil penelitian ini menunjukkan semua kombinasi ekstrak daun petai cina dan kulit jengkol termasuk dalam kategori sangat toksik. Kesimpulan:Kombinasi ekstrak daun petai cina dan kulit jengkol memberikan efek sangat toksik yang diduga berpotensi sebagai antikanker.

scholarly journals South Africa’s Best BARK Medicines Prescribed at the Johannesburg Muthi Markets for Skin, Gut, and Lung Infections: MIC’s and Brine Shrimp Lethality

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 681
Author(s):  
Gugulethu P. Khumalo ◽  
Nicholas J. Sadgrove ◽  
Sandy F. Van Vuuren ◽  
Ben-Erik Van Wyk
Keyword(s):  
South Africa ◽  
Brine Shrimp ◽  
Bark Extract ◽  
Respiratory Pathogens ◽  
Medicinal Uses ◽  
Brine Shrimp Lethality ◽  
Sclerocarya Birrea ◽  
Lung Infections ◽  
Tract Infections

Indigenous trade of medicinal plants in South Africa is a multi-million-rand industry and is still highly relevant in terms of primary health care. The purpose of this study was to identify today’s most traded medicinal barks, traditionally and contemporaneously used for dermatological, gastrointestinal, and respiratory tract infections; then, to investigate the antimicrobial activity and toxicity of the respective extracts and interpret outcomes in light of pharmacokinetics. Thirty-one popularly traded medicinal barks were purchased from the Faraday and Kwa Mai-Mai markets in Johannesburg, South Africa. Information on the medicinal uses of bark-based medicines in modern commerce was recorded from randomly selected traders. The minimum inhibitory concentration (MIC) method was used for antimicrobial screening, and brine shrimp lethality was used to determine toxicity. New medicinal uses were recorded for 14 bark species. Plants demonstrating some broad-spectrum activities against tested bacteria include Elaeodendron transvaalense, Erythrina lysistemon, Garcinia livingstonei, Pterocelastrus rostratus, Rapanea melanophloeos, Schotia brachypetala, Sclerocarya birrea, and Ziziphus mucronata. The lowest MIC value of 0.004 mg/mL was observed against Staphylococcus epidermidis for a dichloromethane bark extract of E. lysistemon. The tested medicinal barks were shown to be non-toxic against the Artemia nauplii (brine shrimp) bioassay, except for a methanol extract from Trichilia emetica (69.52% mortality). Bacterial inhibition of bark extracts with minimal associated toxicity is consistent with the safety and valuable use of medicinal barks for local muthi market customers. Antimicrobial outcomes against skin and gastrointestinal pathogens are feasible because mere contact-inhibition is required in vivo; however, MIC values against respiratory pathogens require further explaining from a pharmacokinetics or pharmacodynamics perspective, particularly for ingested rather than smoked therapies.

scholarly journals In vivo and in vitro antiplasmodial activities of some plants traditionally used in Guatemala against malaria.

1997 ◽  
Vol 41 (7) ◽  
pp. 1500-1503 ◽  
Author(s):  
F F Franssen ◽  
L J Smeijsters ◽  
I Berger ◽  
B E Medinilla Aldana
Keyword(s):  
Plasmodium Berghei ◽  
Brine Shrimp ◽  
Folk Medicine ◽  
Artemia Salina ◽  
In Vitro Cultures ◽  
Cytotoxic Effects ◽  
Resistant Strains ◽  
Simarouba Glauca

We present an evaluation of the antiplasmodial and cytotoxic effects of four plants commonly used in Guatemalan folk medicine against malaria. Methanol extracts of Simarouba glauca D. C., Sansevieria guineensis Willd, Croton guatemalensis Lotsy, and Neurolaena lobata (L.)R.Br. significantly reduced parasitemias in Plasmodium berghei-infected mice. Dichloromethane fractions were screened for their cytotoxicities on Artemia salina (brine shrimp) larvae, and 50% inhibitory concentrations were determined for Plasmodium falciparum in in vitro cultures. Both chloroquine-susceptible and -resistant strains of P. falciparum were significantly inhibited by these extracts. Of all dichloromethane extracts, only the S. glauca cortex extract was considered to be toxic to nauplii of A. salina in the brine shrimp test.

scholarly journals KAJIAN TOKSISITAS DARI TUBUH BUAH Ganoderma lucidum DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST)

2014 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
Ross Nurul Rohmah ◽  
Nuniek Ina Ratnaningtyas ◽  
Ari Asnani
Keyword(s):  
Toxic Effect ◽  
Fruiting Body ◽  
Brine Shrimp ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
One Stage ◽  
Body Extract

Ganoderma lucidum is polyporus fungi from Basidiomycetes which can be used as traditional medicines. Toxicity test with Brine Shrimp Lethality Test (BST) method using Artemia salina was conducted to find out toxic effect of G.lucidum. An extract would have toxic effect if the LC50 < 1000 µg/ml. The aims of this experiment were to know about the toxicity level from G.lucidum with Brine Shrimp Lethality Test (BST) method and determined the concentration of fruiting body extract of G.lucidum which had the best toxicity effect in LC50. There were two extraction methods used in this experiment, first one stage extraction and then multilevel extraction with hexane, ethyl acetate, and ethanol. Each solvent was made in 1000 ppm, 500 ppm, 250 ppm, and 125 ppm. This process was repeated for three times. This experiment used G.lucidum extract which was tested to A.salina and secunder compound metabolit test from the most toxic G. lucidum was done with phytochemical analysis. The result indicated that fruiting body extract from G.lucidum could kill A.salina because all extract was positively contained alkaloid and terpenoid but negatively contained flavonoid. Fruiting body extract from G.lucidum which had lowest LC50 wass ethyl asetat extract with one stage extraction in concentration 53,70 ppm and highest LC50 was ethanol extract with multilevel extraction in concentration 501,18 ppm.

scholarly journals Skrining antikanker menggunakan metode BST (Brine Shrimp Lethality Test) pada ekstrak metanol daun saga (Abrus precatorius L.) dengan partisi etanol

2018 ◽  
Vol 7 (1) ◽  
pp. 61
Author(s):  
Kurnia Ritma Dhanti
Keyword(s):  
Brine Shrimp ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Abrus Precatorius

Latar belakang : Pengembangan senyawa antikanker dari bahan alami perlu dilakukan untuk meminimalisir efek samping dari penanganan penyakit kanker yang saat ini banyak dilakukan. Suatu senyawa dapat diketahui potensi antikankernya dengan pendekatan menggunakan metode BST (Brine Shrimp Lethality Test). Ekstrak metanol daun saga (Abrus precatorius L.) bersifat toksik terhadap larva A. salina Leach.   Tujuan : Mengetahui bagian teraktif dari ekstrak metanol daun saga yang dipartisi (dipisahkan) menggunakan pelarut etanol.   Metode : Ekstrak metanol daun saga dipartisi dengan pelarut etanol hingga terbentuk bagian larut dan tidak larut. Kedua bagian tersebut diuji menggunakan metode BST dengan 5 kali ulangan dan 3 replikasi yang masing-masing menggunakan 10  ekor larva A.salina.   Hasil : Dari perhitungan didapatkan nilai LC50 bagian larut etanol sebesar 144,544 ppm sedangkan nilai LC50 bagian tidak larut etanol sebesar 151,356 ppm.   Kesimpulan : Bagian larut etanol menyebabkan persentase kematian yang lebih tinggi daripada bagian tidak larut. Nilai LC50 bagian larut etanol lebih rendah dibanding bagian tidak larutnya. Semakin rendah nilai LC50 senyawa, maka semakin berpotensi pula untuk dikembangkan sebagai agen antikanker.   Kata kunci : toksisitas, Abrus precatorius L., Artemia salina Leach., antikanker, partisi. 

scholarly journals UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2019 ◽  
Vol 7 (3) ◽  
pp. 265
Author(s):  
Faradila Y. Karim ◽  
Nickson J Kawung ◽  
Billy Th. Wagey
Keyword(s):  
Cytotoxic Activity ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Bioactive Compound ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Thalassia Hemprichii ◽  
Brine Shrimp Lethality ◽  
A Value ◽  
Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)  

scholarly journals Lethal Concentration 50 (LC50) and Effects of Diuron on Morphology of Brine Shrimp Artemia Salina (Branchiopoda: Anostraca) Nauplii

2015 ◽  
Vol 30 ◽  
pp. 279-284 ◽  
Author(s):  
Najla Mohamed Abu Shaala ◽  
Syaizwan Zahmir Zulkifli ◽  
Ahmad Ismail ◽  
Mohammed Noor Amal Azmai ◽  
Ferdaus Mohamat-Yusuff
Keyword(s):  
Brine Shrimp ◽  
Artemia Salina ◽  
Lethal Concentration

scholarly journals Brine Shrimp Lethality Assay of the Aqueous and Ethanolic Extracts of the Selected Species of Medicinal Plants

Proceedings ◽  
2019 ◽  
Vol 41 (1) ◽  
pp. 47
Author(s):  
Sandeep Waghulde ◽  
Mohan K. Kale ◽  
VijayR. Patil
Keyword(s):  
Medicinal Plants ◽  
Brine Shrimp ◽  
Pharmacological Effects ◽  
Bioactive Components ◽  
Pharmacological Activities ◽  
Brine Shrimp Lethality ◽  
Brine Shrimp Lethality Assay ◽  
Ethanolic Extracts ◽  
Annona Reticulata

The present study was conducted to test for in vivo Brine Shrimp Lethality Assay (BSLA) of the Aqueous and ethanolic extracts of Annona reticulata with Allium fistolisum and Brassica oleraceaeand correlate cytotoxicity results with known pharmacological activities of the plants. Cytotoxicity was evaluated in terms of LC50 (lethality concentration). Ten nauplii were added into three replicates of each concentration of the plant extract. After 24 h the surviving brine shrimp larvae were counted and LC50 was assessed. Results showed that the extracts of Annona reticulata with Allium fistolisumand Brassica oleraceaewere potent against the brine shrimp when compared alone with combined extracts. It indicated that bioactive components are present in these plants that could be accounted for its pharmacological effects. Thus, the results support the uses of these plant species in traditional medicine.

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