Antioxidant and Antimicrobial Activities of Essential Oil and Extracts of Saurauia lantsangensis Hu Root

Antioxidant and antimicrobial activities of the essential oil and n-hexane (HEE), chloroform (CHE), ethyl acetate (EAE), and methanol (MEE) extracts, respectively, from the root of Saurauia lantsangensis Hu were investigated. The GC-MS analysis revealed 39 compounds representing 96.41% of the oil containing T-muurolol (13.85%), acetophenone (7.46%), α-cadinol (6.26%), methyl palmitate (5.36%), n-hexadecanoic acid (4.31%), torreyol (3.69%), and isospathulenol (3.48%) as major components. Antioxidant activities determined by three various testing systems, i. e. DPPH radical scavenging, superoxide anion radical scavenging, and reducing power assay, increased in the order: HEE < CHE < oil < MEE < EAE. CHE, EAE, MEE and oil exhibited a promising antimicrobial effect determined as the diameter of zones of inhibition (13.3 - 16.2, 16.5 - 20.4, 13.5 - 16.6, and 16.5 - 22.7 mm), respectively, along with their respective MIC values (500 - 1000, 125 - 500, 250 - 500, and 250 - 500 μg/ml) against Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli), Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus), and a yeast (Hansenula anomala).

Download Full-text

Antimicrobial and antioxidant properties of novel octa-substituted phthalocyanines bearing (trifluoromethoxy) phenoxy groups on peripheral positions

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424619500068 ◽

2019 ◽

Vol 23 (01n02) ◽

pp. 91-102 ◽

Cited By ~ 10

Author(s):

Nazli Farajzadeh ◽

Hande Pekbelgin Karaoğlu ◽

Mustafa Akin ◽

Neslihan Saki ◽

Makbule Burkut Koçak

Keyword(s):

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Antimicrobial Activities ◽

Aggregation Behavior ◽

Spectroscopic Techniques ◽

Direct Bioautography ◽

Reducing Power Assay ◽

Dpph Radical Scavenging

This study presents a novel phthalonitrile derivative (2) bearing (trifluoromethoxy)phenoxy groups in 4,5 positions. Cyclotetramerization of (2) in [Formula: see text],[Formula: see text]-dimethylaminoethanol (DMAE) gave a series of peripherally octa-substituted metallophthalocyanines (3-Zn, 3-Co and 3-Cu). The newly synthesized phthalocyanines have been characterized by a combination of various spectroscopic techniques. Effects of solvent nature on aggregation behavior of 3-Zn were studied using different solvents such as acetone, CHCl3 and dichloromethane (DCM). In addition, the aggregation behavior of the phthalocyanine complex 3-Zn was examined in DCM at different concentrations ranging from 4 × 10[Formula: see text]–14 × 10[Formula: see text] M. Antimicrobial activities of synthesized compounds were tested by using the thin layer chromotography (TLC)-direct bioautography and disk diffusion methods. In both assays, the molecules showed activity on the tested Gram (+) bacteria. Antioxidant activities of the molecules, on the other hand, were determined by using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and a reducing power assay. The highest activity was obtained with 3-ZnPc for both methods.

Download Full-text

Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthiumL.) Essential Oils and Phenolics

Journal of Chemistry ◽

10.1155/2015/804658 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 35

Author(s):

Kamel Msaada ◽

Nidhal Salem ◽

Olfa Bachrouch ◽

Slim Bousselmi ◽

Sonia Tammar ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Essential Oils ◽

Methanolic Extract ◽

Biological Activities ◽

Reducing Power ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Oil Composition ◽

Antioxidant And Antimicrobial Activities

The aim of this study was to determine the chemical variability of wormwood extracts as affected by the growing region. Antioxidant and antimicrobial activities were also investigated. The essential oil composition variability ofA. absinthiumL. aerial parts collected from four different Tunisian regions was assessed by gas chromatography (GC/FID) and by gas chromatography mass spectrometry (GC/MS). In addition, total polyphenols, flavonoids, and condensed tannins as well as antioxidant, antibacterial, and antifungal activities of methanolic extract and essential oils were undertaken. Chromatographic analysis of wormwood essential oils showed the predominance of monoterpene hydrocarbons represented mainly by chamazulene. RP-HPLC analysis of wormwood methanolic extract revealed the predominance of phenolic acids. Antiradical activity was region-dependant and the methanolic extract of Bou Salem region has the strongest activity (CI50=9.38±0.82 µg/mL). Concerning the reducing power, the methanolic extract of Bou Salem, Jérissa, and Boukornine regions was more active than the positive control. Obtained results of antimicrobial activities showed that wormwood essential oil is endowed with important antibacterial activity which was strongly related to the organoleptic quality of oil which appeared strongly region-dependant.A. absinthiumL. EOs investigated are quite interesting from a pharmaceutical standpoint because of their biological activities.

Download Full-text

ASSESSMENT OF PHYTOCHEMICALS, TOTAL PHENOL, FLAVONOID CONTENT AND IN VITRO ANTIOXIDANT PROPERTY OF LARGE CARDAMOM EXTRACTS

INDIAN DRUGS ◽

10.53879/id.58.10.12627 ◽

2021 ◽

Vol 58 (10) ◽

pp. 34-41

Author(s):

Kanthlal S. K. ◽

Jipnomon Joseph ◽

Bindhu P. Paul ◽

Vijayakumar M ◽

Rema Shree A. B. ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Antioxidant Property ◽

Bioactive Constituents ◽

Preliminary Assessment ◽

Reducing Power Assay ◽

Large Cardamom

Amomum subulatum, commonly known as large or black cardamom, is a commonly used spice in Indian kitchens and is traditionally used to treat various ailments. To add more knowledge about the medicinal values of the fruit, this study was conducted to evaluate the in vitro antioxidant activities of aqueous, methanol, ethanol, hydro alcohol, ethyl acetate, acetone and chloroform extracts of the fruit. Preliminary assessment was done to detect the presence of phytoconstituents using identification tests. The antioxidant activity was measured by employing methods such as diphenylpicrylhydrazyl (DPPH) radical scavenging assay, total antioxidant activity equivalent to ascorbic acid, reducing power assay and superoxide anion scavenging assay. The antioxidant activities were compared with their respective phenol and flavonoid contents. Preliminary assessment revealed that large cardamom fruit is a good source of all the bioactive constituents as well as phenol and flavonoid essential for medicinal values. The extract obtained by polar solvents showed the highest antioxidant efficacy in relation to its phenol content. Also, all the solvent-soluble fractions showed a concentration-dependent antioxidant effect. Results from our study prove that large cardamom can alleviate oxidative stress, suggesting the potential of large cardamom as a functional food

Download Full-text

Extraction, Radical Scavenging and Antimicrobial Activity of Essential Oils of Root of Dryopteris marginalis

Asian Journal of Chemistry ◽

10.14233/ajchem.2019.22109 ◽

2019 ◽

Vol 31 (11) ◽

pp. 2512-2516 ◽

Cited By ~ 1

Author(s):

Md. Zahidul Islam ◽

Jannat Al Foisal ◽

Mahbubur Rahman ◽

Mst. Afsana Mimi ◽

Faridul Islam ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Antioxidant Activities ◽

Chemical Constituents ◽

Total Concentration ◽

Petroleum Ether Extract ◽

Radical Scavenging ◽

Organic Extracts ◽

Zones Of Inhibition ◽

Essential Oil Yield

The aim of the study was to characterize the chemical constituents and determine antibacterial and antioxidant activities of essential oils and three different extracts of Dryopteris marginalis. The root had essential oil yield of 0.36 % (w/w) in which 12 organic compounds representing 97.22% of the root oils were identified. Tyranton was found as the most abundant component with 77.571 % of the total concentration in the essential oil. The zones of inhibition of different organic extracts against the tested bacteria were found in the range of 6.5-15 mm. Pseudomonas was the most vulnerable with MICs of 15.62 μg/mL by both ethyl acetate and petroleum ether extract producing 3.8 mm zone of inhibition. The essential oils extracted from roots of Dryopteris marginalis showed maximum 85.29 % inhibition of radical scavenging at 2 mg/mL concentration. Among all root extracts, methanol extract exhibited 41.11 % inhibition at 2 mg/mL concentration.

Download Full-text

Composition, Antioxidant and Antimicrobial Activities of the Leaf Essential Oil of Machilus Japonica from Taiwan

Natural Product Communications ◽

10.1177/1934578x1200700136 ◽

2012 ◽

Vol 7 (1) ◽

pp. 1934578X1200700

Author(s):

Chen-Lung Ho ◽

Yu-Chang Su

Keyword(s):

Essential Oil ◽

Radical Scavenging ◽

Antimicrobial Activities ◽

Disc Diffusion ◽

Gram Positive Bacteria ◽

Leaf Essential Oil ◽

Inhibition Zones ◽

Main Components ◽

Broth Dilution ◽

Antioxidant And Antimicrobial Activities

The chemical composition, and antioxidant and antimicrobial activities of the essential oil isolated from the leaf of Machilus japonica from Taiwan have been investigated. The essential oil from the fresh leaves was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. A total of 97 compounds were identified, representing 100% of the oil. The main components identified were α-phellandrene (14.5%), α-pinene (12.8%), thymol (12.6%), β-pinene (8.3%), α-terpineol (6.5%) and carvacrol (6.0%). The antioxidant activity of the oil was tested by the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capability test. The results showed that the IC50 was 51.8 μg/mL. The antimicrobial activity of the oil was tested by the disc diffusion and micro-broth dilution methods against ten microbial species. The oil exhibited strong growth suppression against Gram-positive bacteria and yeast, with inhibition zones of 48~54 mm and MIC values of 16.12~32.25 μg/mL, respectively. For the antioxidant and antimicrobial activities of the oil, the active source compounds were determined to be thymol and carvacrol.

Download Full-text

Analgesic and Antioxidant Activities of 4-Phenyl-1,5-benzodiazepin-2-one and Its Long Carbon Chains Derivatives

Journal of Chemistry ◽

10.1155/2019/9043570 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Terence Nguema Ongone ◽

Redouane Achour ◽

Mostafa El Ghoul ◽

Latyfa El Ouasif ◽

Meryem El Jemli ◽

...

Keyword(s):

Antioxidant Activity ◽

Acetic Acid ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Carbon Chain ◽

Immersion Test ◽

Tail Immersion ◽

Reducing Power Assay

The aim of this work is to deepen the pharmacological effect of 4-phenyl-1,5-benzodiazepin-2-one derivatives which have a similar structure to nonionic surfactants: 4-phenyl-1,5-benzodiazepin-2-one is the hydrophilic head, and the carbon chain is hydrophobic tail. The antinociceptive activity of 4-phenyl-1,5-benzodiazepin-2-one derivatives was determined using acetic acid-induced writhing and tail immersion tests. In addition, the in vitro antioxidant activities of the tested derivatives were determined by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and ferric reducing power assay. A single oral administration of these compounds at the doses of 50 and 100 mg/kg significantly reduced the number of abdominal writhes induced by acetic acid injection. Acute pretreatment with 4-phenyl-1,5-benzodiazepin-2-one derivatives at the dose of 100 mg/kg caused a significant increase in the tail withdrawal latency in the tail immersion test. Additionally, a significant scavenging activity in DPPH and reducing power was observed in testing antioxidant assays. Finally, we carried out a study of the antioxidant activity of these derivatives. The results of this study reveal that these compounds have a low antioxidant activity compared to the BHT. It decreases with the polarity of the molecule. The present study suggests that 4-phenyl-1,5-benzodiazepin-2-one derivatives possess potent antinociceptive and antioxidant effects, which suggest that the tested compounds may be useful in the treatment of pain and oxidation disorders.

Download Full-text

Antifungal and Antioxidant Activities of the Essential Oil fromAngelica koreanaNakai

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/398503 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 6

Author(s):

Junghyun Roh ◽

Seungwon Shin

Keyword(s):

Essential Oil ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Minimum Effective Dose ◽

Minimal Inhibitory Concentrations ◽

Oil Fraction ◽

Antioxidant Agents ◽

Main Components ◽

Promising Source

Purpose. The purpose of this study is to determine the antifungal and antioxidant activities of the essential oil fromAngelica koreana.Methods. Essential oil was obtained from the dried roots ofA. koreanaby steam distillation, and its composition was identified by gas chromatography and mass spectrometry (GC-MS). The minimal inhibitory concentrations (MICs) of the oil fraction and its main components were determined by broth dilution assay using common pathogenicAspergillusandTrichophytonspecies. The combined effects of the oils with itraconazole were evaluated using a checkerboard titer test. In addition, 1,1-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging, nitrite inhibition, and reducing power were determined to assess the antioxidant activity of this oil.Results. The essential oil fraction and its main components showed inhibitory activity against all of the tested fungi, with minimal inhibitory concentrations (MICs) of 250–1000 μg/mL. Furthermore, this oil exhibited synergism when combined with itraconazole.Conclusion. In the treatment of infections caused byAspergillusandTrichophytonspecies, combining itraconazole with eitherA. koreanaessential oil or its main components may reduce the minimum effective dose of itraconazole required and, thus, minimize its side effects. In addition, this oil is a promising source of natural antioxidant agents.

Download Full-text

Chemical Composition of Essential Oil and Antioxidant Activities of Curcuma petiolata Roxb. Rhizomes

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.506.393 ◽

2012 ◽

Vol 506 ◽

pp. 393-396 ◽

Cited By ~ 4

Author(s):

A. Thakam ◽

N. Saewan

Keyword(s):

Ascorbic Acid ◽

Essential Oil ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Scavenging Activity ◽

Dpph Radical ◽

Etoac Extract ◽

Dpph Radical Scavenging

Pulverized rhizome of Curcuma peliolata on hydrodistillation, afforded light yellow essential oil in the yield of 0.13% v/w. The chemical constituent of the essential oil was determined by GC-MS. Nine compounds representing 95.34 % of the total oil were identified. The major compounds of the oil were 2-methyl-5-pentanol, 1H-pyrrol-1-amine,2-(4-methoxyphenyl)-n,n,5-trimethyl, and curcumol. The distillated aqueous was extracted with dicloromethane (DCM), ethyl acetate (EtOAc), and n-butanol. The DPPH radical scavenging and reducing power of the extracts were investigated. Curcumin and ascorbic acid were used as standard references. The DPPH radical scavenging activity was decrease in order: curcumin ascorbic acid EtOAc extract > DCM extract > n-butanol extract > aqueous extract. The EtOAc extract showed significantly highest inhibition of the radical scavenging activity with IC50 0.092 ± 0.001 mg/mL (curcumin and ascorbic acid (IC50 0.012 and 0.013 mg/mL, respectively (P<0.05)). While, the DCM extract showed highest reducing power with IC50 = 0.271 mg/mL which lower than that of standard curcumin (IC50 = 0.013 mg/mL) and ascorbic acid (IC50 = 0.002 mg/mL).Introduction

Download Full-text

PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND ANTIMICROBIAL ASSESSMENT OF PLUCHEA INDICA (L.) LESS EXTRACT AS AN ACTIVE INGREDIENT IN NATURAL LOTION BAR

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2021v13i2.41555 ◽

2021 ◽

pp. 51-57

Author(s):

SIRIKHWAN TINRAT

Keyword(s):

Broad Spectrum ◽

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Antimicrobial Activities ◽

Dilution Method ◽

Reducing Ability ◽

Phytochemical Compounds ◽

Antioxidant And Antimicrobial Activities

Objective: To screen phytochemical compounds and study antioxidant and antimicrobial activities of Pluchea indica (L.) Less (PI) leaves extracts for assessment the moisturizing lotion bar formulation containing it’s as a bioactive ingredient. Methods: PI leaves were extracted in different solvents. Crude extracts were screened phytochemical compounds and evaluated antioxidant and antimicrobial activities before formulating to antibacterial moisturizing lotion bar. Results: 95% ethanolic extract displayed high antioxidant activities. DPPH radical scavenging capacity with IC50 was 6.80±0.04 mg/ml (61.37±0.41%) and the ferric reducing ability power was 381.20±0.10 mg AAE/100 gDW. Additionally, 95% ethanolic extract served as a broad spectrum bacteriostatic agents against gram-positive and gram-negative species with an inhibition zone of 6.50±0.00-46.67±0.58 mm. It also was simultaneously as a broad spectrum bactericidal agents (MIC and MBC value of 25-200 mg/ml) by broth micro-dilution method. The screened phytochemical constituents (saponins, tannins, flavonoids, terpenoids, steroids, and alkaloids) in PI leaves extract may be responsible for high biological activities. Thus, 95% ethanolic extract was the most appropriate bioactive ingredient for formulated the product development. Conclusion: The developed moisturizing lotion bar with 0.0041% of 95% ethanolic extract was the most eligible formula that showed good appearance, odor, color, homogeneity, after feel and stability. This extract-containing lotion bar formulation achieved a high level of customer satisfaction by sensory evaluation and development of this specific formulation should be subject to further clinical investigation.

Download Full-text

COMPARATIVE EVALUATION OF ANTIDIABETIC AND ANTIOXIDANT POTENCY OF DIFFERENT EXTRACTS OBTAINED FROM MEMECYLON SPECIES

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15695 ◽

2017 ◽

Vol 9 (2) ◽

pp. 187

Author(s):

Tumkur Ramasetty Bharathi ◽

Harishchandra Sripathy Prakash

Keyword(s):

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Methanol Extracts ◽

Soxhlet Apparatus ◽

Antioxidant Potency ◽

Reducing Power Assay ◽

Different Doses

Objective: Memecylon species is being extensively used in traditional medicine for the treatment of skin disorders and it is proved to possess antidiabetic and anti-inflammatory properties. The present investigation was to study the effect of different solvent extracts of five Memecylon species such as M. umbellatum, M. talbotianum, M. edule, M. malabaricum and M. wightii on antidiabetic and antioxidant effects.Methods: Plant extracts were prepared using soxhlet apparatus using different solvents such as hexane, ethyl acetate, methanol and water and obtained extracts were subjected to antidiabetic (α-amylase and α-glucosidase inhibition assays) and antioxidant (2, 2-Diphenyl-2-Picryl Hydrazyl hydrate (DPPH), 2,2-Azino-bis (3-ethyl benzothiazoline-6-Sulfonic acid)diammonium salt (ABTS), Superoxide radical scavenging assay (SRSA) and reducing power assays) evaluated at different doses.Results: Methanol extracts of all five Memecylon species exhibited effective antidiabetic and antioxidant properties among them methanol extracts of M. malabaricum and M. talbotianum have highest biological activity. For α-amylase IC50 value for both M. malabaricum and M. talbotianum was found to be 100 and 130 µg/reaction and IC50 value for α-glucosidase was found to be 6.1 and 7.8 µg/reaction respectively. For DPPH the IC50value was found to be 190 µg/reaction, for ABTS 31-39 µg/reaction, for SRSA 950-1200 µg/reaction and for reducing power assay 420-490 µg/reaction respectively.Conclusion: The results indicate that methanol extracts of M. malabaricum and M. talbotianum possess potent in vitro antidiabetic and antioxidant activities compared to other Memecylon species.

Download Full-text