Polymorphism of Glutathione S-transferase Genes and the Risk of Toxic Liver Damage in Petrochemical Workers

Background: Exposure to numerous chemicals, including industrial ones, may result in liver damage. The body susceptibility to the environmental hazards largely depends on the activity of the enzymes in the xenobiotic detoxification system. Function abnormalities of such enzymes due to genetic variations would increase the risk of developing various diseases. Objective: To elucidate the relationship between polymorphism in glutathione S-transferase genes (GSTM1, GSTT1 and GSTP1) and the risk of toxic liver damage in a group of petrochemical workers. Methods: This study was conducted on 72 workers with toxic liver injury, 156 healthy workers, and 322 healthy individuals without history of occupational exposure to chemicals. Genotyping of the GSTP1 rs1695 gene polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Polymerase chain reaction (PCR) was used to perform genotyping of the GSTM1 and GSTT1 genes polymorphism. Results: There was a significant difference in genotype frequencies of the GSTP1 rs1695 gene polymorphism among the groups studied. The distribution of Val/Val genotype of the GSTP1 rs1695 gene polymorphism had a higher incidence in healthy workers compared with patients with toxic liver damage (p=0.036). No significant association was found between the GSTM1 and GSTT1 polymorphisms and toxic liver damage. Conclusion: The GSTP1 rs1695 gene polymorphism can play a protective role in the development of toxic liver damage in petrochemical workers.

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Genetic polymorphism of drug metabolism enzymes (GSTM1, GSTT1 and GSTP1) in the healthy Malian population

Molecular Biology Reports ◽

10.1007/s11033-019-05143-5 ◽

2019 ◽

Vol 47 (1) ◽

pp. 393-400 ◽

Cited By ~ 1

Author(s):

Yaya Kassogue ◽

Brehima Diakite ◽

Oumar Kassogue ◽

Issa Konate ◽

Kadidiatou Tamboura ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Healthy Population ◽

Statistical Association ◽

Genetic Associations ◽

Glutathione S Transferase ◽

Chain Reaction ◽

Multifactorial Diseases ◽

Genotype Frequencies ◽

Metabolism Enzymes ◽

Polymerase Chain

Abstract Glutathione S-transferase genes, known to be highly polymorphic, are implicated in the process of phase II metabolism of many substrates, including xenobiotics, anticancer and anti-infective drugs. The detoxification activity is linked to individual genetic makeup. Therefore, the identification of alleles and genotypes in these genes within a population may help to better design genetic susceptibility and pharmacogenetic studies. We performed the present study to establish the frequencies of the GSTM1, GSTT1, and GSTP1 c. 313A > G (rs1695) polymorphisms in 206 individuals of the Malian healthy population. GSTM1 and GSTT1 were genotyped by using multiplex polymerase chain reaction, whereas genotypes of GSTP1 were identified by polymerase chain reaction followed by restriction fragment length polymorphism. The frequencies of GSTM1-null and GSTT1-null genotypes were respectively 24.3 and 41.3%. The observed genotype frequencies for GSTP1 were 25.73% homozygous wild-type AA, 49.03% heterozygous AG and 25.24% homozygous mutant GG. The frequency of GSTP1-A allele was 50.24% versus 49.76% for the GSTP1-G allele. The distribution of these three genes was homogeneous between men and women (p > 0.05). We found no statistical association between the presence of a particular profile of GSTM1 or GSTT1 with the genotypes of GSTP1 (p > 0.05). Nevertheless, we noticed that the majority of the individuals harboring the GSTM1-present or the GSTT1-present harbor also the GSTP1-AG genotype. In addition, the triple genotype GSTM1-present/GSTT1-present/AG was the most frequent with 25.2%. Our findings will facilitate future studies regarding genetic associations of multifactorial diseases and pharmacogenetic, thus opening the way to personalized medicine in our population.

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Evaluation of the relationship between IL-12, IL-13 and TNF-α gene polymorphisms with the susceptibility to brucellosis: a case control study

BMC Infectious Diseases ◽

10.1186/s12879-019-4678-8 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 1

Author(s):

Sima Kazemi ◽

Asad Vaisi-Raygani ◽

Fariba Keramat ◽

Massoud Saidijam ◽

Ali Reza Soltanian ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Gene Polymorphism ◽

Gene Polymorphisms ◽

Serum Levels ◽

Enzyme Linked Immunosorbent Assay ◽

Chain Reaction ◽

Genotype Frequencies ◽

Tnf Α ◽

Polymerase Chain ◽

The Relationship

Abstract Background The cytokine gene polymorphism is important for the genetic susceptibility of infectious diseases. The aim of the present study was to investigate the relationship between TNF-α, IL-12, and IL-13 gene polymorphisms and predisposition to brucellosis. Methods In this study, 107 patients with brucellosis and 107 healthy individuals were evaluated. The SNPs of TNF-α)- 238 G/A) and IL-12 (+ 1188 A/C) were done by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) and IL-13 genotyping at positions − 1512 (A/C) and − 1112 (C/T) were analysis by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) methods. IL-12, IL-13 and TNF-α serum levels were measured by a sandwich enzyme-linked immunosorbent assay (ELISA). Results IL-13 (−1512A/C) was associated with Brucellosis risk in dominant model (OR (95% CI) = 2.17 (1.02–4.62)), P-value = 0.041. However, there was no difference in allele and genotype frequencies of TNF-α)- 238 G/A), IL-12 (+ 1188 A/C) and IL-13 [− 1512 (A/C) and − 1112 (C/T)] between patients and controls. Serum levels of IL-12 and TNF-α were significantly more frequent in the patients than in the control groups. Conclusions The IL-13 gene polymorphism can be used as a biomarker for detecting susceptibility to Brucella disease.

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Critical analysis: use of polymerase chain reaction to diagnose leprosy

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502016000100018 ◽

2016 ◽

Vol 52 (1) ◽

pp. 163-169 ◽

Cited By ~ 1

Author(s):

Flaviane Granero Maltempe ◽

Vanessa Pietrowski Baldin ◽

Mariana Aparecida Lopes ◽

Vera Lúcia Dias Siqueira ◽

Regiane Bertin de Lima Scodro ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Globin Gene ◽

Public Health Problem ◽

Reference Laboratory ◽

Chain Reaction ◽

Molecular Biology Technique ◽

Pcr Inhibitor ◽

Significant Difference ◽

Polymerase Chain ◽

Pcr Techniques

ABSTRACT Leprosy is a neglected tropical disease and an important public health problem, especially in developing countries. It is a chronic infectious disease that is caused by Mycobacterium leprae, which has a predilection for the skin and peripheral nerves. Although it has low sensitivity, slit-skin smear (SSS) remains the conventional auxiliary laboratory technique for the clinical diagnosis of leprosy. Polymerase chain reaction (PCR) is a molecular biology technique that holds promise as a simple and sensitive diagnostic tool. In the present study, the performance of two PCR methods, using different targets, PCR-LP and PCR-P, were compared with SSS with regard to leprosy diagnosis in a reference laboratory. M. leprae DNA was extracted from 106 lymph samples of 40 patients who had clinical suspicion of leprosy. The samples were subjected to both PCR techniques and SSS. Amplification of the human b-globin gene was used as PCR inhibitor control. The specificity of both PCR techniques was 100%, and sensitivity was 0.007 and 0.015 µg/ml for PCR-LP and PCR-P, respectively. No significant difference was found between either the PCR-LP or PCR-P results and SSS results (p > 0.05). Although PCR is not yet a replacement for SSS in the diagnosis of leprosy, this technique may be used as an efficient auxiliary tool for early detection of the disease, especially in endemic regions. This strategy may also be useful in cases in which SSS results are negative (e.g., in paucibacillary patients) and cases in which skin biopsy cannot be performed.

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Study of the HLA-DPβ1 Locus by the Polymerase Chain Reaction Technique in Patients with Hodgkin's Disease

Tumori Journal ◽

10.1177/030089169307900211 ◽

1993 ◽

Vol 79 (2) ◽

pp. 133-136 ◽

Cited By ~ 2

Author(s):

Guseppe Pellegris ◽

Claudia Lombardo ◽

Annelisa Cantoni ◽

Liliana Devizzi ◽

Monica Balzarotti

Keyword(s):

Polymerase Chain Reaction ◽

Hodgkin's Disease ◽

Hodgkin’S Disease ◽

Polymerase Chain Reaction Method ◽

Healthy Controls ◽

Chain Reaction ◽

Reaction Method ◽

Significant Difference ◽

Polymerase Chain

Background A number of reports have studied associations between Hodgkin's disease and HLA. Some of them established correlation between several antigens and Hodgkin's disease, and others found no correlations. Methods The HLA DP locus was determined by the polymerase chain reaction method in 31 Hodgkin's disease patients and 58 healthy controls. Results No significant difference between patients and controls was noted. Conclusions Further investigations are needed to confirm the hypothesis of a possible role of the HLA complex as one of the factors involved in Hodgkin's disease.

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Association between Platelet-Specific Collagen Receptor Glycoprotein 6 Gene Variants, Selected Biomarkers, and Recurrent Pregnancy Loss in Korean Women

Genes ◽

10.3390/genes11080862 ◽

2020 ◽

Vol 11 (8) ◽

pp. 862

Author(s):

Hui Jeong An ◽

Eun Hee Ahn ◽

Jung Oh Kim ◽

Chang Soo Ryu ◽

Han Sung Park ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Confidence Interval ◽

Odds Ratio ◽

Pregnancy Loss ◽

Adjusted Odds Ratio ◽

Recurrent Pregnancy Loss ◽

Korean Women ◽

Chain Reaction ◽

Genotype Frequencies ◽

Polymerase Chain

This paper investigates whether glycoprotein 6 (GP6) gene polymorphisms are a risk factor for recurrent pregnancy loss (RPL) in Korean women. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism and real-time polymerase chain reaction amplification. We identified five polymorphisms in the GP6 gene: rs1654410 T>C, rs1671153 T>G, rs1654419 G>A, rs12610286 A>G, and rs1654431 G>A. GP6 rs1654410 CC was associated with decreased RPL risk (adjusted odds ratio = 0.292, 95% confidence interval = 0.105–0.815, p = 0.019), and recessive genotypes were also significantly associated with decreased RPL risk (adjusted odds ratio = 0.348, 95% confidence interval = 0.128−0.944, p = 0.038). GP6 rs1654419 GA was associated with decreased RPL risk (adjusted odds ratio = 0.607, 95% confidence interval = 0.375-0.982, p = 0.042), and dominant genotypes were significantly associated with decreased RPL risk (adjusted odds ratio = 0.563, 95% confidence interval = 0.358−0.885, p = 0.013). Altogether, the genotype frequencies of GP6 rs1654410 T>C and GP6 rs1654419 G>A were significantly different between RPL patients and control participants. Therefore, although GP6 polymorphisms may be useful as biomarkers of RPL, additional studies with heterogeneous cohorts are required to better understand the influence of GP6 and assess its performance as a biomarker.

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Low frequency of CYP2A6 gene polymorphism as revealed by a one-step polymerase chain reaction method

Pharmacogenetics ◽

10.1097/00008571-199906000-00007 ◽

1999 ◽

Vol 9 (3) ◽

pp. 327-332 ◽

Cited By ~ 37

Author(s):

Gen-Fu Chen ◽

Yong-Ming Tang ◽

Bridgett Green ◽

Dong-Xin Lin ◽

F. Peter Guengerich ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Gene Polymorphism ◽

Low Frequency ◽

Polymerase Chain Reaction Method ◽

Chain Reaction ◽

Reaction Method ◽

One Step ◽

Polymerase Chain ◽

Cyp2a6 Gene ◽

Step Polymerase Chain Reaction

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Impact of Respiratory Viral Panel Polymerase Chain Reaction Assay Turnaround Time on Length of Stay and Antibiotic Use in Patients With Respiratory Viral Illnesses

Hospital Pharmacy ◽

10.1177/0018578717731573 ◽

2017 ◽

Vol 52 (9) ◽

pp. 640-644 ◽

Cited By ~ 3

Author(s):

Sebastian Choi ◽

Rubiya Kabir ◽

Pranisha Gautam-Goyal ◽

Prashant Malhotra

Keyword(s):

Polymerase Chain Reaction ◽

Length Of Stay ◽

Retrospective Chart Review ◽

Antibiotic Use ◽

Turnaround Time ◽

Chain Reaction ◽

Time Period ◽

Significant Difference ◽

Before And After ◽

Polymerase Chain

Background: Respiratory viral illnesses account for many hospitalizations and inappropriate antibiotic use. Respiratory viral panels by polymerase chain reaction (RVP-PCR) provide a reliable means of diagnosis. In 2015, the RVP-PCR assay at our institution was switched from respiratory viral panel (RVP) to rapid respiratory panel (rapid RP), which has a faster turnaround time (24 hours vs 12 hours, respectively). The purpose of this study was to evaluate the effect of RVP-PCR tests on duration of antibiotic use and length of stay (LOS) in hospitalized patients. Methods: We performed a retrospective chart review of patients who had a RVP-PCR ordered within a 1-year time period before and after the assay switch. Patients who were pregnant, had received antibiotics within 30 days prior to admission, were not discharged, or had not completed antibiotics by end of study period were excluded. Results: Data were obtained from a total of 140 patients (70 in each group). Of these, 25 (35.7%) in the RVP group and 28 (40.0%) in the rapid RP group had a positive result. The median LOS was 4.5 days (IQR, 3-9 days) in the RVP group and 5 days (IQR, 3-9 days) in the rapid RP group ( P = .78). The median duration of antibiotic use was 4 days (IQR, 2-7 days) in the RVP group and 5 days (IQR, 1-7 days) in the rapid RP group ( P = .8). Conclusion: Despite faster turnaround time, there was no significant difference in duration of antibiotic use, or LOS between the RVP and rapid RP groups.

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Polymorphisms of LHβ and GnRHR genes and their association with the number of embryos recovered in goats

Animal Production Science ◽

10.1071/an13076 ◽

2014 ◽

Vol 54 (8) ◽

pp. 987 ◽

Cited By ~ 1

Author(s):

M. Z. Fu ◽

G. Li ◽

Z. Q. Zhou

Keyword(s):

Polymerase Chain Reaction ◽

Single Nucleotide Polymorphisms ◽

Gene Polymorphism ◽

Corpus Luteum ◽

Nucleotide Polymorphisms ◽

Chain Reaction ◽

Single Strand Conformational Polymorphism ◽

Single Nucleotide ◽

Exon 2 ◽

Polymerase Chain

The objective of the present study was to explore a predictor of superovulation response on the basis of associations between the number of embryos recovered and gene polymorphism. Variation in the goat LHβ and GnRHR genes was investigated using polymerase chain reaction–single-strand conformational polymorphism and DNA sequencing. Two single nucleotide polymorphisms (SNPs) were identified in the 5′-UTR of LHβ gene (A59C, P1 locus) and in the Exon 2 of GnRHR gene (T177A, P6 locus). At the P1 locus in both breeds, the frequencies of one allele were 0.46 and 0.51, respectively. At the P6 locus, the minor allele frequency was 0.23. Associations of both SNPs with the number of embryos recovered and the corpus luteum number were evaluated in Boer and Shaanbei goat breeds. Association analysis showed that both SNPs had significant (P < 0.05) effects on the number of embryos recovered and corpus luteum number. These results indicate that LHβ and GnRHR genes are potential markers for the number of embryos recovered.

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MTHFRGene C677T Mutation andACEGene I/D Polymorphism in Turkish Patients with Osteoarthritis

Disease Markers ◽

10.1155/2013/658654 ◽

2013 ◽

Vol 34 (1) ◽

pp. 17-22 ◽

Cited By ~ 9

Author(s):

Ahmet Inanir ◽

Serbulent Yigit ◽

Sengul Tural ◽

Osman Cecen ◽

Eren Yildirim

Keyword(s):

Polymerase Chain Reaction ◽

Methylenetetrahydrofolate Reductase ◽

Chain Reaction ◽

Gene Insertion ◽

Significant Difference ◽

Joint Disorder ◽

Allele Specific ◽

C677t Mutation ◽

Polymerase Chain ◽

Turkish Patients

Osteoarthritis is a degenerative joint disorder resulting in destruction of articular cartilage, osteophyte formation, and subchondral bone sclerosis. In recent years, numerous genetic factors have been identified and implicated in osteoarthritis. The aim of the current study was to examine the influence of methylenetetrahydrofolate reductase (MTHFR) gene C677T mutation and angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) variations on the risk of osteoarthritis.Genomic DNA is obtained from 421 persons (221 patients with osteoarthritis and 200 healthy controls).ACEgene I/D polymorphism genotypes were determined using polymerase chain reaction using I and D allele-specific primers. TheMTHFRC677T mutation was analyzed by polymerase chain reaction (PCR) based restriction fragment length polymorphism (RFLP) methods. We found significant difference between the groups with respect to bothACEandMTHFRgenotype distributions (p< 0.001,p< 0.001 respectively). Our study suggests thatACEgene DD genotype andMTHFRgene CC genotype could be used as genetic markers in osteoarthritis in Turkish study populations.

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