scholarly journals RapID Cell Counter: Semi-automated and mid-throughput estimation of cell density within diverse cortical layers

eNeuro ◽  
2021 ◽  
pp. ENEURO.0185-21.2021
Author(s):  
Aarthi Sekar ◽  
Thiago M. Sanches ◽  
Keiko Hino ◽  
Matangi Kumar ◽  
Juliann Wang ◽  
...  
Keyword(s):  
Cell Density ◽  
Cortical Layers ◽  
Cell Counter

scholarly journals Routine Management of Microalgae Using Autofluorescence from Chlorophyll

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4441 ◽  
Author(s):  
Toshiyuki Takahashi
Keyword(s):  
Cell Density ◽  
Environmental Changes ◽  
Algal Species ◽  
Working Hours ◽  
Industrial Applications ◽  
Density Analysis ◽  
Application Potential ◽  
Further Development ◽  
Automated Cell Counter ◽  
Cell Counter

From a high-potential biomass perspective, microalgae have recently attracted considerable attention due to their extensive application in many areas. Although studies searching for algal species with extensive application potential are ongoing, technical development for their assessment and maintenance of quality in culture are also critical and inescapable challenges. Considering the sensitivity of microalgae to environmental changes, management of algal quality is one of the top priorities for industrial applications. Helping substitute for conventional methods such as manual hemocytometry, turbidity, and spectrophotometry, this review presents an image-based, automated cell counter with a fluorescence filter to measure chlorophyll autofluorescence emitted by algae. Capturing chlorophyll-bearing cells selectively, the device accomplished precise qualification of algal numbers. The results for cell density using the device with fluorescence detection were almost identical to those obtained using hemocytometry. The automated functions of the device allow operators to reduce working hours, for not only cell density analysis but simultaneous multiparametric analysis such as cell size and algal status based on chlorophyll integrity. The automated device boldly supports further development of algal application and might contribute to opening up more avenues in the microalgal industry.

No evidence for altered cell density in cortical layers 3 and 5 of Heschl's gyrus in schizophrenia

2003 ◽  
Vol 60 (1) ◽  
pp. 69
Author(s):  
D.R. Cotter ◽  
D. Mackay ◽  
P. Falkai ◽  
C. Beasley ◽  
I. Everall
Keyword(s):  
Cell Density ◽  
Cortical Layers ◽  
Heschl’S Gyrus ◽  
Heschl's Gyrus ◽  
Altered Cell

Validation of cell density and viability assays using Cedex automated cell counter

Biologicals ◽  
2010 ◽  
Vol 38 (3) ◽  
pp. 393-400 ◽  
Author(s):  
Li-Chun Huang ◽  
Wendy Lin ◽  
Machiko Yagami ◽  
Daphne Tseng ◽  
Emily Miyashita-Lin ◽  
...  
Keyword(s):  
Cell Density ◽  
Automated Cell Counter ◽  
Cell Counter

Increassed parietal cell density in patients with perfurated duodenal ulcer: Comparison with non-complicated duodenal ulcer patients

2001 ◽  
Vol 120 (5) ◽  
pp. A250-A250
Author(s):  
A BARBOSA ◽  
C MENDES ◽  
L COELHO ◽  
C RODRIGUES ◽  
M MACHADO ◽  
...  
Keyword(s):  
Duodenal Ulcer ◽  
Cell Density ◽  
Parietal Cell

Comparative Study of Size, Total Protein, Fibrinogen and 5-HT Content of Human and Canine Platelet Density Subpopulations

1986 ◽  
Vol 56 (03) ◽  
pp. 288-292 ◽  
Author(s):  
Diego Mezzano ◽  
Eduardo Aranda ◽  
Arnaldo Foradori
Keyword(s):  
Comparative Study ◽  
Protein Content ◽  
Cell Density ◽  
Total Protein ◽  
Unit Volume ◽  
Low Density ◽  
Dense Bodies ◽  
Artifactual Changes ◽  
Platelet Release ◽  
The Difference

SummaryThe size, total protein, fibrinogen and 5-HT content were evaluated in density subpopulations of human and canine platelets fractionated in linear arabinogalactan gradients. The methodology was assessed to ascertain that platelet separation was by density and to discard artifactual changes and platelet release during the procedure. EDTA or PGEi increased the size of human PRP-platelets, but not of dog platelets. In humans, high density (HD) platelets were 1.26 times larger and contained 1.88 times more fibrinogen, 2.23 times more 5-HT and 1.37 times more protein than low density (LD) platelets; in dogs, these density cohorts did not differ in protein content, but LD platelets were 1.29 times larger and had 1.33 times more fibrinogen and 5-HT than HD platelets. These findings suggest that cell density is mostly dependent on the protein content per unit volume of platelets (and not on dense bodies). The differences in fibrinogen and 5-HT content between HD and LD cohorts in humans and dogs may be related to platelet age. The difference in volume between HD and LD platelets in dogs is of uncertain interpretation.

Maternal high fat diet (HFD) in the adult offspring brain modifies cell density and neuronal density

2016 ◽  
Author(s):  
Diego Ojeda Pedraza ◽  
Kate Jane-Coupe ◽  
Megan Earl ◽  
Oliver Hutton ◽  
Judith Eckert ◽  
...  
Keyword(s):  
Cell Density ◽  
High Fat Diet ◽  
Adult Offspring ◽  
High Fat ◽  
Neuronal Density

Changes in the Population Density of Pathogenic Microorganisms in Response to the Allelopathic Effect of Thypha Latifolia

2014 ◽  
Vol 1 (1) ◽  
pp. 31-36 ◽  
Author(s):  
O. Zhukorskiy ◽  
O. Gulay ◽  
V. Gulay ◽  
N. Tkachuk
Keyword(s):  
Cell Density ◽  
Allelopathic Effect ◽  
Pathogenic Microorganisms ◽  
Sterile Water ◽  
Erysipelothrix Rhusiopathiae ◽  
Pathogenic Leptospira ◽  
Favorable Conditions ◽  
And Control ◽  
The Impact ◽  
Control Samples

Aim. To determine the response of the populations of Erysipelothrix rhusiopathiae and Leptospira interrogans pathogenic microorganisms to the impact of broadleaf cattail (Thypha latifolia) root diffusates. Methods. Aqueous solutions of T. latifolia root diffusates were sterilized by vacuum fi ltration through the fi lters with 0.2-micron pore diameter. The experimental samples contained cattail secretions, sterile water, and cultures of E. rhusiopathiae or L. interrogans. The same amount of sterile water, as in the experimental samples, was used for the purpose of control, and the same quantity of microbial cultures was added in it. After exposure, the density of cells in the experimental and control samples was determined. Results. Root diffusates of T. latifolia caused an increase in cell density in the populations of E. rhusiopathiae throughout the whole range of the studied dilutions (1:10–1:10000). In the populations of the 6 studied serological variants of L. interrogans spirochetes (pomona, grippotyphosa, copenhageni, kabura, tarassovi, canicola), the action of broadleaf cattail root diffusates caused the decrease in cell density. A stimulatory effect was marked in the experimental samples of the pollonica serological variant of leptospira. Conclusions. The populations of E. rhusiopathiae and L. interrogans pathogenic microorganisms respond to the allelopathic effect of Thypha latifolia by changing the cell density. The obtained results provide the background to assume that broadleaf cattail thickets create favorable conditions for the existence of E. rhusiopathiae pathogen bacteria. The reduced cell density of L. interrogans in the experimental samples compared to the control samples observed under the infl uence of T. latifolia root diffusates suggests that reservoirs with broadleaf cattail thickets are marked by the unfavorable conditions for the existence of pathogenic leptospira (except L. pollonica).

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