Utility of automated cell counter histograms in reporting peripheral smears in anemia

From a high-potential biomass perspective, microalgae have recently attracted considerable attention due to their extensive application in many areas. Although studies searching for algal species with extensive application potential are ongoing, technical development for their assessment and maintenance of quality in culture are also critical and inescapable challenges. Considering the sensitivity of microalgae to environmental changes, management of algal quality is one of the top priorities for industrial applications. Helping substitute for conventional methods such as manual hemocytometry, turbidity, and spectrophotometry, this review presents an image-based, automated cell counter with a fluorescence filter to measure chlorophyll autofluorescence emitted by algae. Capturing chlorophyll-bearing cells selectively, the device accomplished precise qualification of algal numbers. The results for cell density using the device with fluorescence detection were almost identical to those obtained using hemocytometry. The automated functions of the device allow operators to reduce working hours, for not only cell density analysis but simultaneous multiparametric analysis such as cell size and algal status based on chlorophyll integrity. The automated device boldly supports further development of algal application and might contribute to opening up more avenues in the microalgal industry.

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Potential of an Automated- and Image-Based Cell Counter to Accelerate Microalgal Research and Applications

Energies ◽

10.3390/en13226019 ◽

2020 ◽

Vol 13 (22) ◽

pp. 6019

Author(s):

Toshiyuki Takahashi

Keyword(s):

Sustainable Development ◽

Management System ◽

Sustainable Development Goals ◽

Environmental Changes ◽

Development Goals ◽

Conventional Methods ◽

Functional Capacities ◽

Automated Cell Counter ◽

Cell Counter ◽

Work Burden

Efforts to achieve Sustainable Development Goals (SDGs) have resulted in enhancement of the position of microalgae in feedstocks for food, feed, healthcare, and biofuels. However, stabile microalgal biorefineries require a sustainable and reliable management system of microalgae, which are sensitive to environmental changes. To expand microalgal applicability, assessment and maintenance of microalgal quality are crucial. Compared with conventional methods, including hemocytometry and turbidity, an automated- and image-based cell counter contributes to the establishment of routine management of microalgae with reduced work burden. This review presents the principle of an automated cell counter and highlights the functional capacities of the device for microalgal management. The method utilizing fluorescence function to evaluate the chlorophyll integrity of microalgae may lay the groundwork for making a large variety of microalgal biorefineries, creating an important step toward achieving SDGs.

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Robust automated cell counter and viability measurement for engineered T cell product release

Cytotherapy ◽

10.1016/j.jcyt.2018.02.193 ◽

2018 ◽

Vol 20 (5) ◽

pp. S69

Author(s):

Y. Ohayon ◽

C.M. Corl ◽

R.M. Leskowitz ◽

A. Malykhin ◽

S.M. Arostegui ◽

...

Keyword(s):

T Cell ◽

Cell Product ◽

Product Release ◽

Automated Cell Counter ◽

Cell Counter

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Platelet satellitism found by an abnormal leukocyte cytogram with an automated cell counter

American Journal of Hematology ◽

10.1002/ajh.2830490325 ◽

1995 ◽

Vol 49 (3) ◽

pp. 261-263

Author(s):

Osamu Kabutomori ◽

Yoshinori Iwatani ◽

Taichin Koh ◽

Nobuyuki Amino

Keyword(s):

Platelet Satellitism ◽

Automated Cell Counter ◽

Cell Counter

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Umbilical Cord Blood (UCB) Banking: Is Performing the Quality Controls on a Thawed Cryovial Representative of the UCB Graft?.

Blood ◽

10.1182/blood.v104.11.3643.3643 ◽

2004 ◽

Vol 104 (11) ◽

pp. 3643-3643

Author(s):

Laura Salvaneschi ◽

Cesare Perotti ◽

Paola Bergamaschi ◽

Claudia Del Fante ◽

Gianluca Viarengo ◽

...

Keyword(s):

Clinical Use ◽

Growing Up ◽

Hematopoietic Stem ◽

Quality Controls ◽

Inhibiting Factor ◽

Transplant Center ◽

Cd34 Cells ◽

Gentle Agitation ◽

Automated Cell Counter ◽

Cell Counter

Abstract Introduction: Nowadays UCB represents an established source of hematopoietic stem cells for unrelated transplants in children and the employ in adults is quickly growing up. Nucleated cells (NCs) content is one of the main predictors to evaluate UCB for clinical use; however, other indicators of the hematopoietic potential, such as CD34+ cell and colony-forming cells (CFUs), have recently showed similar correlations. According to Netcord-FACT standards, it is recommended to test all the above mentioned parameters before releasing UCB to the transplant center; a segmented tubing of the UCB bag should be used but a satellite cryotube is more often available. We preliminarily report the results of quality controls performed on thawed cryovials corresponding to each of 15 units delivered by our UCB Bank. Material and methods: in our policy, all UCBs are stored accompanied by 3 satellite cryovials, treated under the same conditions of the unit. For each of the 15 UCBs released for transplantation, one cryotube was thawed in a 37°C water bath with a gentle agitation, without washing out DMSO. NCs and mononucleated cells (MNCs) were estimated with an automated cell counter. Viability and CD34+ cell count were evaluated by flow cytometry, with a no-wash, single-platform technique and 7-aminoactinomycin D. CFU assay was performed using commercial reagents (Methocult GF H4434, StemCell Technologies) and colonies were classified after a 14 days incubation. The same parameters referring to fresh UCBs (before cryopreservation) were always available. Results: the UCB characteristics measured after thawing a cryovial were compared with those of the fresh cord and are detailed in the table below. fresh UCB before cryopreservation UCB cryovial after thawing Yield (%) NC (x106) 1491.3 (148–2262) 1354.2 (167.4–2119.9) 92 (83–113) MNC (x106) 662.7 (96.5–900.3) 638.8 (295–1238.7) 95 (63–159) CD 34+ cells (x106) 3.47 (0.38–10.87) 3.19 (0.4–9.27) 85 (37–118) Viability (%) 96 (88–100) 62 (39–77) Viability of CD34+ cells (%) 92 (71–99) CFU-GM (x104) 838.5 (57.2–3581.2) 424.11 (65.29–917.64) 69 (16–184) BFU-E (x104) 1709.61 (159.84–5116) 473.34 (39.69–1204.14) 41 (5–139) total CFU (x104) 2565.17 (217.04–8953) 911.68 (164.43–2075.22) 57 (9–160) Excellent yields were found for NCs, MNCs and CD34+ cells. Despite of the decrease in the overall viability, the viable CD34+ cells as percentage was always highly satisfactory. The colonies growth was found lower in the thawed sample in comparison with fresh UCB before cryopreservation. Conclusion: in our experience, highly satisfactory evaluations of UCB content could be obtained using thawed cryotubes with regard to NC, MNC and also CD34+ cell. However, concerning the latter, the different methods employed on fresh UCBs, such as CD34+ cells detection without beads, may be advocated to explain some discrepancies in the yield range. The results of CFU assay confirmed to be poorly useful, essentially because affected by a subjective interpretation even if the reduced cell growth may be also related to the presence of DMSO as inhibiting factor.

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The role of phosphoglycerate mutase 2 in UM-UC3 bladder cancer cell metabolism.

Journal of Clinical Oncology ◽

10.1200/jco.2019.37.7_suppl.410 ◽

2019 ◽

Vol 37 (7_suppl) ◽

pp. 410-410

Author(s):

Ryan Didde ◽

Weiya Liu ◽

Karim Pirani ◽

Gaurav Kaushik ◽

Benjamin L. Woolbright ◽

...

Keyword(s):

Diabetes Mellitus ◽

Bladder Cancer ◽

Cell Growth ◽

Western Blot ◽

Differential Expression ◽

Control Cell ◽

Phosphoglycerate Mutase ◽

Patients With Diabetes ◽

Automated Cell Counter ◽

Cell Counter

410 Background: Bladder cancer remains the fourth most common cancer in American males with a higher risk of recurrence and progression for patients with diabetes mellitus. Urothelial bladder cancer is characterized by aerobic glycolysis with upregulation of glycolytic enzymes (known as the Warburg effect) such as phosphoglycerate mutase. Phosphoglycerate mutase 2 (PGAM2), a reversible glycolytic enzyme expressed highly in muscle, represents a target for modulation because of its differential expression from another isoform, phosphoglycerate mutase 1. PGAM2 knockdown may impact bladder cancer growth significantly via its effect on glucose metabolism at different glucose concentrations seen in patients with diabetes mellitus. Methods: UM-UC3 bladder cancer cells were assessed for PGAM2 expression at different glucose concentrations via Western blot and quantitative PCR. One native UM-UC3 line, three PGAM2 knockdown lines, and one vector control cell line were included in the western blot study. Cellular proliferation was analyzed using an enzyme based hexoseaminidase assay and was further supported with an automated cell counter. The effects of cisplatin were also investigated. Results: Increased PGAM2 expression at increased glucose concentrations in UM-UC3 was confirmed by Western blot and quantitative PCR. PGAM2 knockdown cells responded differently to changes in glucose concentration compared to the control cell lines, with a large increase in growth at a low glucose level of 25mg/dL after day 4. Cell proliferation demonstrated similar growth between the knockdown and controls at higher glucose concentrations of 100 and 200mg/dL. Proliferation data using automated cell counter demonstrate the same growth trend. Conclusions: Increased cell growth of PGAM2 knockdowns, most notably at 25mg/dL, suggests that PGAM2 may play a different role in glycolysis than expected, possibly serving as a modulator in cell growth instead of a simple reversible enzyme. We are currently investigating its differential expression compared to PGAM1, an enzyme recently characterized to have an opposite effect to PGAM2.

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