scholarly journals KULTIVASI SPIRULINA PLATENSIS PADA MEDIA BERNUTRISI LIMBAH CAIR TAHU DAN SINTETIK

2017 ◽  
Vol 5 (2) ◽  
pp. 68-73
Author(s):  
Iqbal Syaichurrozi ◽  
Jayanudin Jayanudin
Keyword(s):  
Growth Rate ◽  
Protein Content ◽  
Growth Medium ◽  
Optical Density ◽  
Spirulina Platensis ◽  
Good Growth ◽  
Cultivation Time ◽  
C:N:P Ratio

This research was conducted to study the effect of various additions of synthetic nutrient into Spirulina platensis growth medium containing tofu wastewater 8%v/v. Synthetic nutrient was varied at 50, 75, 100%. Based on abservation, the OD680 (Optical Density at 680 nm) increased until in the end of cultivation time with final OD680 of 0.381, 0.392, 0.189 for variable of 50, 75, 100% respectively. Meanwhile, the growth rate value was 0.0743, 0.0759, 0.0354/day. The best protein content was obtained at nutrient synthetic of 75%, which was 91,27%. The good growth and protein content of S. platensis obtained at nutrient synthetic of 75%, which had C:N:P ratio of 128:12:1.

scholarly journals Effect of Land Agricultural Fertilizer on Growth of Marine Single Cell Protein, Spirulina platensis, Chlorella vulgaris and Nannochloropsis

Omni-Akuatika ◽  
2019 ◽  
Vol 15 (2) ◽  
pp. 69
Author(s):  
Purnama Sukardi ◽  
Ferisa Wahyunika ◽  
Tjahyo Winanto ◽  
Norman Arie Prayogo ◽  
Taufan Harisam ◽  
...  
Keyword(s):  
Growth Rate ◽  
Single Cell ◽  
Protein Content ◽  
Chlorella Vulgaris ◽  
Spirulina Platensis ◽  
Single Cell Protein ◽  
Nannochloropsis Oculata ◽  
Cell Protein ◽  
C 30 ◽  
Agricultural Fertilizer

This study aimed to determine the growth rate and protein content in Nannochloropsis oculata, Chlorella vulgaris, Spirulina platensis were cultured using agricultural fertilizers. The agricultural fertilizers used were Urea, ZA and TSP. Each single cell protein was cultured using the three types of fertilizers with a ratio of Urea: ZA and TSP as follows (A) 1: 2: 1 [(10:20:10 g / L)], (B) 2: 2: 1 [(20:20:10 g / L)] and (C) 3: 2: 1 [(30:20:10 g / L)], respectively. The results showed that the best ratio of Urea, ZA and TSP fertilizers to growth of Spirulina, Nannochloropsis oculata and Chlorella vulgaris was C [30:20:10 (g / L)] treatment. However, phyto-protein content in Nannochlorophsis differed significantly between fertilization treatments, C fertilization yielded the highest protein content (28.75±0.05%), when compared with A (25.13±0.01%) and B (25.14±0.02%), respectively. In Chlorella vulgaris, all fertilization treatments showed very significant differences, B fertilization (28.24%±00.006) yielded the highest phyto-protein content, if compared to A (23.63% ± 0.003) and C (19.74% ±0.006 ), respectively. All fertilization treatments showed very significant differences (P <0.05) on Spirulina platensis. The highest content of phyto-protein (62.68 + 0.05%) was present in treatment C, when compared to A (52.18 + 0.05%) and B (62.37 + 0.01%) treatments, respectively.

scholarly journals Pengaruh Pertumbuhan Spirulina sp. terhadap Penggunaan Pupuk Organik Cair sebagai Media Tumbuh

2020 ◽  
Vol 5 (4) ◽  
pp. 202
Author(s):  
Risa Swandari Wijihastuti ◽  
Akifah Lutfiah ◽  
Nita Noriko
Keyword(s):  
Growth Rate ◽  
Protein Content ◽  
Growth Medium ◽  
Growth Analysis ◽  
Organic Liquid ◽  
Organic Fertilizer ◽  
Growth Media ◽  
Liquid Fertilizer ◽  
Growing Medium ◽  
Media Concentration

<p><em>Abstrak</em> - <strong>Spirulina sp. merupakan mikroalga yang mengandung antioksidan, phytonutrient, probiotik dan nutraceuticals yang dapat meningkatkan kandungan protein makanan dengan pencampuran biomassa Spirulina sp. kering dengan tepung ganyong atau biasa disebut Cannalina. Peningkatan penggunaan tepung ganyong dapat meningkatkan limbah tajuk tanaman ganyong yang tidak ikut terolah menjadi tepung. Pemanfaatan limbah tajuk ganyong tersebut dapat diolah menjadi pupuk organik yang dapat dimanfaatkan menjadi media tumbuh mikroalga khususnya Spirulina sp.. Tujuan dari penelitian ini adalah untuk melihat pengaruh pertumbuhan Spirulina sp. terhadap pemberian pupuk organik hasil fermentasi limbah tajuk tanaman Ganyong sebagai media tumbuh. Penelitian ini akan dilakukan dengan pembuatan pupuk organik cair, persiapan bibit kultur Spirulina sp., persiapan media tumbuh dan analisa pertumbuhan dari Spirulina sp.. Penelitian ini diharapkan dapat memperoleh luaran berupa publikasi pada jurnal nasional terakreditasi. Pupuk cair organik yang dihasilkan dari fermentasi tajuk tanaman ganyong telah memenuhi kriteria pupuk organik cair yang yang diterbitkan pada Peraturan Menteri Pertanian NO:37/Permentan/SR.130/5/2010 sehingga dapat diaplikasikan pada tanaman. Laju pertumbuhan dan produksi biomassa Spirulina sp. mencapai angka tertinggi pada konsentrasi media pupuk organik 14 mL/L. Penggunaan pupuk organik cair dari tajuk tanaman Ganyong berpotensi menjadi alternatif bahan media pertumbuhan Spirulina sp.</strong></p><p><em>Abstract</em> – <strong>Spirulina sp. is a microalga that contains antioxidants, phytonutrients, probiotics, and nutraceuticals that can increase the protein content of food by mixing the dried Spirulina sp. with Ganyong flour which is called Cannalina. Increasing the use of Ganyong flour can increase waste from the plant shoot that is not processed into flour. Utilization of the shoot waste can be processed into organic fertilizer which can be used as a growing medium for Spirulina sp. The objective of this study is to find the effect of Spirulina sp. growth on the organic fertilizer medium that is resulting from Ganyong shoot fermentation. This research will be carried out by making liquid organic fertilizer, preparation of Spirulina sp. culture stock, growth media, and growth analysis. Organic liquid fertilizer produced from the Ganyong shoot fermentation has met the criteria for liquid organic fertilizer published in Minister of Agriculture Regulation NO: 37 / Permentan / SR.130 / 5/2010 so that it can be applied to plants. The growth rate and biomass production of Spirulina sp. reached the highest rate at 14 mL / L organic fertilizer media concentration. The use of liquid organic fertilizer from Canna plant shoot has the potential to be an alternative growth medium for Spirulina sp..</strong></p><p><em><strong>Keywords</strong> - Spirulina sp., Microalgae, Ganyong, Waste, Pupuk Organik Cair</em></p>

Toxic Effects of Quinalphos on Protein Content and Growth Rate in Freshwater Teleost, Cyprinus carpio (L.)

2016 ◽  
Vol 23 (1) ◽  
pp. 89
Author(s):  
A. Padmanabha ◽  
H. R. V. Reddy ◽  
N. B. Shridhar ◽  
Muttappa Khavi ◽  
B. T. Naveen Kumar
Keyword(s):  
Growth Rate ◽  
Protein Content ◽  
Cyprinus Carpio ◽  
Toxic Effects ◽  
Freshwater Teleost

scholarly journals Incorporation of Spirulina platensis on Traditional Greek Soft Cheese with Respect to Its Nutritional and Sensory Perspectives

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 99
Author(s):  
Loulouda Bosnea ◽  
Antonia Terpou ◽  
Eleni Pappa ◽  
Efthymia Kondyli ◽  
Marios Mataragas ◽  
...  
Keyword(s):  
Protein Content ◽  
Spirulina Platensis ◽  
Dairy Products ◽  
Consumer Acceptance ◽  
Constant Weight ◽  
High Protein Content ◽  
Microbiological Profile ◽  
Soft Cheese ◽  
Evaluation Panel ◽  
Cheese Production

Spirulina platensis, the most popular microalgae species known for its high protein content and bioactive compounds such as phycocyanin and allophycocyanin, has been studied for cheese fortification. Incorporation of spirulina in dairy products poses major sensorial challenges due to its characteristic odor and its insolubility in food formulation, thus limiting consumer acceptance. The main objective was the production of a novel spread cheese fortified with spirulina, so powdered spirulina was added at different concentrations (0.25, 0.5, and 1%), and the effect on physicochemical, microbiological, and sensory characteristics was assessed. Cheese samples were examined for pH, fat (Gerber-Van Gulik method), salt (Volhard method), protein (Kjeldahl), and moisture content by drying to constant weight at 102 ± 1 °C. Cheeses were also assessed organoleptically by five experienced panelists. Generally, the addition of spirulina slightly increased the protein content and affected the color of the cheeses. The cheeses achieved a good microbiological profile and were all characterized as acceptable for consumption by the panelists. However, the cheeses with 0.25 and 0.5% spirulina were mostly preferred by the evaluation panel due to the less intense characteristic odor and taste of spirulina. We conclude that it is possible to produce an acceptable spread cheese with the addition of spirulina without significant changes in the cheese production line.

scholarly journals Biological Impact of Photoperiod on Fairy Shrimp (Branchinecta orientalis): Life History and Biochemical Composition

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 695
Author(s):  
Sara Farhadi ◽  
Behrooz Atashbar Kangarloei ◽  
Ahmad Imani ◽  
Kourosh Sarvi Moghanlou
Keyword(s):  
Growth Rate ◽  
Life History ◽  
Protein Content ◽  
Biochemical Composition ◽  
Digestive Enzyme ◽  
Total Body Length ◽  
Constant Darkness ◽  
Fairy Shrimp ◽  
Daily Growth ◽  
Isoleucine Leucine

B. orientalis, fairy shrimp, is often among the most conspicuous invertebrates inhabiting temporary aquatic habitats with a typical variation in environmental conditions. Its life history characteristics and biochemical composition were studied under four different photoperiodic regimes (24L:0D, 0L:24D, 16L:8D, and 12L:12D). The significantly highest cumulative and initial hatching rates (48 h) were obtained at 24L:0D (p < 0.05). Cultivating the larvae under different photoperiods did not significantly affect specific growth rate (SGR) (p > 0.05). However, higher final total body length and daily growth rate were recorded under constant darkness. Higher lipid content was found at 24L:0D to the extent that it was more than two times higher than that at 16L:8D and 12L:12D (p < 0.05). There was also a remarkable increase in body crude protein content at 24L:0D (p < 0.05). Body fatty-acid profiles of the fairy shrimps were also affected by culture condition (p < 0.05). Extension of lighting period resulted in a subtle increase in body contents of arginine, lysine, histidine, isoleucine, leucine, valine, methionine, and phenylalanine, especially in the group kept under a 16L:8D regime. The highest and lowest digestive enzyme activity was observed at 0L:24D and 24L:0D, respectively (p < 0.05). In contrast, the highest and lowest soluble protein content was recorded at 24L:0D and 0L:24D, respectively (p < 0.05). Similarly, antioxidant status was significantly higher at 0L:24D (p < 0.05). In conclusion, a 16L:8D light–dark cycle might be an optimal condition in terms of growth performance and physio-biochemical characteristics. These findings could be helpful in optimizing the rearing conditions for upscaling B. orientalis production.

scholarly journals The ameliorative effect of selenium on Azolla caroliniana grown under UV-B stress

2015 ◽  
Vol 95 (1) ◽  
pp. 20-26 ◽  
Author(s):  
E.M. Mostafa ◽  
A.M.A. Hassan
Keyword(s):  
Growth Rate ◽  
Protein Content ◽  
Doubling Time ◽  
The Other ◽  
Total Glutathione ◽  
Nutrient Media ◽  
Relative Growth ◽  
Other Hand ◽  
Ameliorative Effect ◽  
Glutathione Redox

Exposure ofAzollaplants to UV-B radiation for 6 h resulted in a decrease in biomass and relative growth rate (RGR), which coincided with an increase in doubling time (DT) as compared with the control. Also, the protein content decreased. On the other hand, hydrogen peroxyde (H2O2) and malondialdehyde (MDA) accumulated significantly in UV-treatedAzollaplants. Conversely, the addition of selenium (Se) at 1 ppm resulted in a significant increase in biomass and protein content of untreated and UV-treatedAzollaplants, and a significant reduction in both H2O2and MDA. Moreover, the addition of Se to UV-treated and untreatedAzollaplants resulted in a significant increase in total ascorbate and total glutathione (GSH) contents compared with the control and UV-stressedAzollaplants. Also, glutathione redox potential (GSH/TG) increased significantly in UV-treatedAzollaplants in the presence of Se. There also was a significant increase (38%) in ascorbate peroxidase (APX) activity in UV-treated plants compared with the control. APX activity in the presence of Se did not change significantly compared with the control. Glutathione reductase (GR) activity increased significantly in UV-treatedAzolla, while glutathione peroxidase (GSH-PX) activity did not. On the other hand, both GSH-PX and GR activity in untreated and UV-treatedAzollaplants were significantly enhanced by the application of Se to the nutrient media at a concentration of 1 ppm. Therefore, we can conclude that Se protectsAzollaplants from UV-B stress.

scholarly journals Quantitative Interaction Effects of Carbon Dioxide, Sodium Chloride, and Sodium Nitrite on Neurotoxin Gene Expression in Nonproteolytic Clostridium botulinum Type B

2004 ◽  
Vol 70 (5) ◽  
pp. 2928-2934 ◽  
Author(s):  
Maria Lövenklev ◽  
Ingrid Artin ◽  
Oskar Hagberg ◽  
Elisabeth Borch ◽  
Elisabet Holst ◽  
...  
Keyword(s):  
Gene Expression ◽  
Carbon Dioxide ◽  
Growth Rate ◽  
Sodium Chloride ◽  
Optical Density ◽  
Sodium Nitrite ◽  
Clostridium Botulinum ◽  
Lag Phase ◽  
Type B ◽  
Phase Duration

ABSTRACT The effects of carbon dioxide, sodium chloride, and sodium nitrite on type B botulinum neurotoxin (BoNT/B) gene (cntB) expression in nonproteolytic Clostridium botulinum were investigated in a tryptone-peptone-yeast extract (TPY) medium. Various concentrations of these selected food preservatives were studied by using a complete factorial design in order to quantitatively study interaction effects, as well as main effects, on the following responses: lag phase duration (LPD), growth rate, relative cntB expression, and extracellular BoNT/B production. Multiple linear regression was used to set up six statistical models to quantify and predict these responses. All combinations of NaCl and NaNO2 in the growth medium resulted in a prolonged lag phase duration and in a reduction in the specific growth rate. In contrast, the relative BoNT/B gene expression was unchanged, as determined by the cntB-specific quantitative reverse transcription-PCR method. This was confirmed when we measured the extracellular BoNT/B concentration by an enzyme-linked immunosorbent assay. CO2 was found to have a major effect on gene expression when the cntB mRNA levels were monitored in the mid-exponential, late exponential, and late stationary growth phases. The expression of cntB relative to the expression of the 16S rRNA gene was stimulated by an elevated CO2 concentration; the cntB mRNA level was fivefold greater in a 70% CO2 atmosphere than in a 10% CO2 atmosphere. These findings were also confirmed when we analyzed the extracellular BoNT/B concentration; we found that the concentrations were 27 ng · ml−1 · unit of optical density−1 in the 10% CO2 atmosphere and 126 ng · ml−1 · unit of optical density−1 in the 70% CO2 atmosphere.

Genetic relationships between wood quality traits and diameter growth of juvenile core wood in Sitka spruce

2013 ◽  
Vol 43 (1) ◽  
pp. 1-6 ◽  
Author(s):  
S.G. Kennedy ◽  
A.D. Cameron ◽  
S.J. Lee
Keyword(s):  
Growth Rate ◽  
Genetic Correlation ◽  
Modulus Of Elasticity ◽  
Genetic Relationships ◽  
Wood Quality ◽  
Wood Properties ◽  
Sitka Spruce ◽  
Acoustic Velocity ◽  
Picea Sitchensis ◽  
Good Growth

The trend towards shorter rotations in planted conifer stands has resulted in a reduction in the proportion of mature wood relative to juvenile core wood, raising concerns that the mechanical performance of sawn battens will be affected. The potential to improve the wood quality of the juvenile core of Sitka spruce (Picea sitchensis (Bong.) Carrière) without compromising growth rate was investigated. Rapid and inexpensive indirect methods of assessing wood properties on standing trees using a Pilodyn gun and acoustic velocity were compared with direct measurements made on wood samples cut from the juvenile core. Strong genetic correlations were observed between Pilodyn gun values and direct measures of density (–0.76) and between the square of acoustic velocity and modulus of elasticity (0.73). The genetic correlation between the square of acoustic velocity and microfibril angle was also strong (–0.84). These results suggest that indirect assessments of wood properties within juvenile core wood are sufficiently reliable for these techniques to be used in the Sitka spruce breeding programme. Although a strong negative genetic correlation between diameter at breast height and density was noted (–0.79), sufficient variation exists within the breeding population to select families with both good growth rate and high modulus of elasticity wood.

scholarly journals Komparasi Agribisnis Jamur Tiram (Pleurotus ostreatus) Media Tanam Limbah Sabut Kelapa dan Serbuk Gergaji

2021 ◽  
Vol 5 (2) ◽  
pp. 108
Author(s):  
D Yadi Heryadi ◽  
Ristina Siti Sundari ◽  
Rini Agustini ◽  
Andang Hidayat
Keyword(s):  
Protein Content ◽  
Growth Medium ◽  
Pleurotus Ostreatus ◽  
Production Cost ◽  
Animal Protein ◽  
High Protein ◽  
Net Income ◽  
Total Cost ◽  
High Protein Content ◽  
Oyster Mushrooms

The agribusiness of oyster mushrooms (Pleurotus ostreatus) is getting interested due to an increase in demand. It has almost complete nutrients, high protein content that can substitute animal protein for vegetarians. The growth medium for oyster mushrooms commonly uses sawdust. The cocopeat waste medium is an innovation. The research descriptively compares production cost, yield, revenue, income, and feasibility throughout both mediums. Findings that the total cost of sawdust medium was higher 750,000IDR than cocopeat waste. In terms of income, sawdust medium earned 140.72%, while cocopeat waste earned 133.29%. The feasibility of sawdust waste was 2.41 while cocopeat waste was 2.33 as well. Otherwise, the cocopeat waste medium was higher 99.21IDR than sawdust waste in cost/kg yield. Net income using cocopeat waste medium was 2,739,694.50IDR, and cocopeat waste was 2,511,769.50IDR on average. Both cocopeat waste and sawdust waste medium are very feasible to be an agribusiness. The consideration is that the availability surrounds the production site, and cocopeat waste is cheaper than sawdust waste. Agribisnis jamur tiram (Pleurotus ostreatus) semakin diminati oleh karena permintaan terus meningkat. Kandungan gizinya lengkap dengan protein yang tinggi. Bahkan bagi vegetarian menjadi pengganti protein hewani. Media pertumbuhan jamur tiram biasanya menggunakan limbah serbuk gergaji. Media limbah serbuk sabut kelapa merupakan inovasi baru. Penelitian ini ingin mengetahui biaya-biaya hingga kelayakan usaha agribisnis jamur tiram menggunakan media limbah serbuk sabut kelapa dan yang biasa yaitu limbah serbuk gergaji Ternyata, Biaya total setahun menggunakan media limbah serbuk sabut kelapa lebih hemat Rp750.000 dibandingkan dengan media limbah serbuk gergaji. Biaya per kilogramnya sedikit lebih mahal Rp99.21 tetapi hasil panen lebih rendah dari limbah serbuk gergaji. Jamur tiram media limbah serbuk sabut kelapa memberikan keuntungan 133.29% dan media limbah serbuk gergaji 140.72%. Jamur tiram media limbah serbuk gergaji memberikan keuntungan bersih/bulan rata-rata Rp2,739,694.50, sedangkan media limbah serbuk sabut kelapa rata-rata Rp2,511,769.50.Kedua media untuk jamur tiram sangat layak diusahakan dengan R/C ratio 2.33 dan 2.41. Pengusaha bisa mempertimbangkan media limbah serbuk sabut kelapa atau limbah serbuk gergaji tergantung ketersediaan di sekitar lokasi dengan informasi harga limbah sabut kelapa lebih murah daripada limbah serbuk gergaji.

scholarly journals EVALUATION OF ARTHROSPIRA SP. GROWTH ABILITY ON HEAVY METAL SALTS AND THEIR EFFECT ON SOME CELLULAR COMPONENTS

2020 ◽  
Vol 17 (34) ◽  
pp. 667-677
Author(s):  
Hiba Khaleel Saeed AL-SHAKARCHI ◽  
Yousef Jabbar AL-SHAHERY
Keyword(s):  
Growth Rate ◽  
Protein Content ◽  
Biomass Concentration ◽  
Local Strain ◽  
Food Supplement ◽  
Metal Salts ◽  
Chlorophyll Contents ◽  
Copper Nickel ◽  
Iron Copper ◽  
Nickel Cadmium

rthrospira sp. is an aquatic and photosynthetic microorganism that is extensively employed as a food supplement due to its rich contents of nutrients, proteins, and carbohydrates. In this study, a local strain of cyanobacterium of the genus Arthrospira was isolated from the Iraqi soil, in the region of Mosul city, using the Chu’s Medium No. 10. The growth rate, as well as the effects on biomass and cellular component contents of proteins, carbohydrates, and chlorophyll of this strain, were evaluated on the molasses medium alone or supplemented with iron, copper, nickel, cadmium and cobalt salts after fifteen days of incubation. The results showed that the best growth rate (1.09 OD), the highest value of biomass (120.0 mg/l), proteins content (297.2 mg/l), chlorophyll content (14.9 mg/l) and carbohydrates content (400.0 mg/l) of Arthrospira sp. was achieved after fifteen days of incubation. Generally, it was observed that adding iron, copper, nickel, cadmium, and cobalt salts into the molasses medium increased the contents of biomass, proteins, and carbohydrates of Arthrospira sp.. It was noticed that the highest biomass concentration (1960 mg/l) was obtained when Arthrospira sp. grown on molasses medium supplemented with nickel. Also, none of the metal salts added to the molasses medium increased the protein content of Arthrospira sp.. Conversely, adding copper, nickel, and cobalt to the medium showed an adverse effect on the protein content. It was shown that adding iron metal salts into the molasses medium increased the carbohydrates and the chlorophyll contents of Arthrospira sp.. These results suggest that Arthrospira sp. can be utilized for the bioremediation of heavy metals pollution in the environment and industrial sites.

Sign in / Sign up

Export Citation Format

Share Document