scholarly journals Effects of Storage Duration and Temperature on the Values of Haematological Parameters in Bovine and Ovine Blood Samples

2018 ◽  
Vol 68 (2) ◽  
pp. 145
Author(s):  
A. H. KIRMIZIGUL ◽  
E. GOKCE ◽  
M. SOZMEN
Keyword(s):  
Venous Blood ◽  
Blood Parameters ◽  
The Other ◽  
Haematological Parameters ◽  
Bovine Blood ◽  
Storage Duration ◽  
Blood Samples ◽  
Study Material ◽  
Other Hand ◽  
Haematological Values

The purpose of the present study was to determine the alterations in haematological values at 0, 12, 24, 36, 48, 60, 72, 84 and 96 hours of storage in bovine and ovine venous blood samples stored at 4˚C and 24˚C in EDTA-coated tubes. Twenty healthy animals, including ten 4-year-old cattle and ten 2-year-old sheep constituted the study material. Bovine blood samples stored at 4˚C produced reliable results for the WBC, RBC, Hb, PCV, MCV, MCH and MCHC levels with the exception of PLT level as PLT levels decreased when the PLT stored 24 hours or longer times. On the other hand ovine blood samples stored at 4˚C for 24 hours or longer, all the parameters measured (WBC, RBC, Hb, PCV, MCV, MCH, MCHC and PLT levels) gave reliable results indicating that ovine blood parameters can be used effectively and safely. Furthermore, in the bovine blood samples, MCHC levels were decreased as from 60 hours stored at 24°C and WBC levels were decreased in the ovine blood samples stored 48 hours or longer at 24°C. However, the blood samples stored at 24°C, the measurement of the RBC, Hb, PCV, MCV, MCH and PLT levels produced reliable results.

CORTICOTROPIN-BESTIMMUNG ANHAND DES CORTICOSTERONANSTIEGES IM NEBENNIEREN-VENENBLUT HYPOPHYSEKTOMIERTER RATTEN

1962 ◽  
Vol 41 (2) ◽  
pp. 211-218 ◽  
Author(s):  
K. Retiene ◽  
H. Ditschuneit ◽  
M. Fischer ◽  
K. Kopp ◽  
E. F. Pfeiffer
Keyword(s):  
Peripheral Blood ◽  
Single Injection ◽  
Venous Blood ◽  
Corticosterone Level ◽  
The Other ◽  
Adrenal Vein ◽  
Major Surgery ◽  
Other Hand ◽  
Hypophysectomized Rats

ABSTRACT Corticotrophin has been measured by using the corticotrophin-induced increase of corticosterone in adrenal venous blood of rats, the corticotrophin secretion of which has been blocked by preliminary injection of dexamethasone. Sensitivity and precision of this technique have not been higher than in the simpler procedure using corticosterone increase in peripheral blood. Single injection of dexamethasone on the other hand did not prevent release of endogenous corticotrophin following major surgery, required for canulation of the adrenal vein. In hypophysectomized rats corticotrophin can be measured by using adrenal venous blood. 0.05 mU corticotrophin (US-P-Standard) has been determined with an index of precision of λ = 0.13. The consistent relation between initial and elevated corticosterone level following corticotrophin in both peripheral and adrenal venous blood makes it highly unlikely that other modifications of this kind of assay will increase sensitivity.

Interpretation of 25-OH-D3, NEFA, and calcium correlations among cow and calf pairs

2025 ◽  
Vol 77 (04) ◽  
pp. 6511-2025
Author(s):  
SONGUL ERDOGAN ◽  
KEREM URAL ◽  
HASAN ERDOGAN ◽  
DENIZ ALIC URAL ◽  
MEHMET GULTEKIN ◽  
...  
Keyword(s):  
Energy Balance ◽  
Vitamin D3 ◽  
Colorimetric Method ◽  
Negative Energy ◽  
The Other ◽  
Negative Energy Balance ◽  
Test Results ◽  
Blood Samples ◽  
Other Hand ◽  
Cow Calf

In the present study, the aim was to determine alteration of NEFA, calcium and vitamin D3 levels in cow and calf pairs at parturition as well as correlation between each parameter levels. For this purpose, a cow-side device employing the enzymatic colorimetric method was used for measurement of NEFA and calcium levels. On the other hand, serum 25(OH)D3 analysis was performed using the fluorescence immunochromatographic method at the laboratory in the Faculty. Blood samples were taken from Vena jugularis of 15 Simmental cow and calf pairs immediately after parturition and placed in serum and heparinized tubes. In cow and calf pairs, concentration of vitamin D3 (15.6-120 and 31.8-120 ng/mL, respectively), NEFA (0.12-1.2 and 0.09-0.8 mmol/L, respectively) and calcium (1.8 ± 0.9 and 2.2 ± 0.6 mmol/L, respectively) were determined. There was no significant correlation between NEFA, Ca, and vitamin D3 in cows and calf pairs. Taking into account several co-factors that influenced test results, which could not easily be excluded, further studies may be warranted with larger cow-calf pair populations. In conclusion, vitamin D3 concentration in calves is not affected by the negative energy balance of dams in the parturition period.

scholarly journals Changes in prevalence and abundance of parasitic invasions in sheep at various physiological stages

2017 ◽  
Vol 73 (11) ◽  
pp. 713-716
Author(s):  
Paulina Dudko ◽  
Andrzej Junkuszew ◽  
Krzysztof Tomczuk
Keyword(s):  
Environmental Conditions ◽  
Management System ◽  
Animal Husbandry ◽  
Gastrointestinal Parasites ◽  
The Other ◽  
Prevention Strategies ◽  
Pasture Management ◽  
Study Material ◽  
Other Hand

The aim of the study was to determine the degree of parasitic invasion at various physiological stages, as well as tracing the dynamics of parasitic invasions in a flock of sheep kept in the combined indoor-pasture management system. The observations were performed on 224 mother ewes of the synthetic SCP prolific meat line over the course of two years. The animals were kept in the combined indoor-pasture management system with uniform feeding and environmental conditions and were under continuous animal husbandry and veterinary supervision. The ewes participating in the experiment were not wormed in the year preceding the experiment and during the observations. The experiment involved parasitologic tests aimed at the determination of abundance and prevalence of invasions of gastrointestinal parasites in mother ewes. The study material was faeces collected from the rectum in the following stages: pregnancy (November), day 2, 28, 42 and 56 after lambing, drying off (day 100 after lambing) and rest (between dry-off and mating). In the analysis of the results of the study it can be noted that, in most cases, the highest prevalence and abundance levels are observed in the infertility period. Moreover, the period of pregnancy and lambing was a crucial one. On the other hand, the lowest abundance and prevalence levels in the majority of cases were observed on day 28 and 42 after lambing. The results of the study should be taken into consideration during the development of prevention strategies limiting parasitic invasions in sheep flocks. Adjusting chemotherapy to the most important periods of parasitic invasions will enable a more efficient fight against parasites. It should also reduce the excessive use of chemical preparations, which is crucial due to the increasing resistance of parasites. Apart from the aforementioned actions, farmers should pay attention to supporting immunity by means of feed, especially in the periods of highest vulnerability to invasions. Such actions should lead to the reduction of losses caused by parasitic invasions in sheep flocks.

X-IRRADIATION OF RIBOSE AND DEOXYRIBOSE DERIVATIVES

1962 ◽  
Vol 40 (1) ◽  
pp. 1553-1565
Author(s):  
D. K. Myers
Keyword(s):  
Aqueous Solution ◽  
Nucleic Acids ◽  
The Other ◽  
Free Sugar ◽  
Blood Samples ◽  
Other Hand ◽  
Dilute Aqueous Solution ◽  
X Irradiation ◽  
In Cells

During X-irradiation of ribosides and deoxyribosides in dilute aqueous solution, purine and sugar moieties were destroyed at approximately the same rate. Pyrimidine moieties, on the other hand, were destroyed more rapidly, resulting in a fairly rapid liberation of free sugar during irradiation of pyrimidine ribosides or deoxyribosides. No marked differences in the rates of destruction of ribose and deoxyribose derivatives were observed in these experiments.Similar reactions were observed during irradiation of blood samples. However, acid-soluble ribonucleotides in the blood proved to be much more sensitive than the nucleic acids or proteins to destruction by X-radiation. The significance of these findings is discussed with relation to the damage produced in cells by X-irradiation.

scholarly journals Effect of different doses of uranyl acetate on some blood parameters and hepatic enzymesin females rats.

2014 ◽  
Vol 8 (3) ◽  
pp. 37-43
Author(s):  
Sarah Ghazi Alzorii
Keyword(s):  
Oral Administration ◽  
Liver Enzyme ◽  
Blood Parameters ◽  
Uranyl Acetate ◽  
The Other ◽  
Control Group ◽  
Albino Rats ◽  
Distilled Water ◽  
Blood Samples ◽  
Different Doses

This study was carried out to investigate effects of oral administration of uranyl acetate on haematological parameters and liver enzyme. For this purpose, twenty females mature Albino rats were divided into four equal groups, control group G1 administrated distilled water, the other three groups administrated orally 50,75,100 mg/kg/b.w. /day of uranyl acetate. The route of administration was oral intubations for 10 weeks, for 14 days before mating with untreated males, as well as during pregnancy 3 weeks and lactation 5 weeks. At the end of the treatment blood samples were collected from mothers of rats. The results were obtained a significant P< 0.05 increase in the total WBC counts in groups G2,G3,G4 compared with control .A significant P<0.05 decrease in the lymphocytes and monocytes percentage in groups (G3,G4), while no significant decrease P>0.05 in G2 in the lymphocytes and monocytes percentage compared with control. And the results show significant P< 0.05 decrease in HB value in groups G3,G4, while no significant decrease P>0.05 in G2 in HB value compared with control. A significant P< 0.05 decrease was observed in total RBCs counts in groups G2,G3,G4 compared with control. No significant increase P>0.05 in GOT and GPT in groups G2 and G3 and significant P<0.05 increase in GOT and GPT activity in the serum in group G4 compared with control. No significant increase P>0.05 in CPK activity in groups G2,G3,G4 compared with control.

scholarly journals Effects of Feeding Diets Containing Pearl Millet Supplemented with Enzyme on the Carcass and Haematological Values of Broiler Chicken

2021 ◽  
pp. 52-60
Author(s):  
John Amedu Edache ◽  
Lydia Inyam ◽  
Stephen Ejembi Edache
Keyword(s):  
Pearl Millet ◽  
Broiler Chickens ◽  
Energy Content ◽  
Control Diet ◽  
Blood Parameters ◽  
Metabolizable Energy ◽  
The Other ◽  
Control Group ◽  
Haematological Values ◽  
Feeding Diets

Pearl millet, which is comparable to maize in terms of its metabolizable energy content is an identified potential replacement for maize in broiler diets. However, as the competition and price of maize continues to spike, the continuous need to completely replace maize in broiler diets is imperative. Therefore, the objectives of this study were to investigate the effects of feeding diets containing pearl millet supplemented with enzyme NatuzymeTM on the carcass characteristics and haematological values of broiler chickens. 150 two weeks-old broilers were randomly divided into 5 groups with 10 birds per pen translating into 10 birds per replicate and fed an isonitrogenous (23%CP) starter diet of control diet (A); 0% (B); 10% (C); 20% (D); 30.01% and (E) 37.41 % pearl millet. Similarly, an isonitrogenous (21%CP) finisher diet of control diet (A); 0% (B); 10.64% pearl millet (C); 21.28% (D); 31.92% and (E) 39.27 % of pearl millet for 6 weeks. The birds were randomly allotted to the diets in a completely randomized design with feed and water given ad libitum. With the exception of drumstick of birds fed diet D which was significantly lower than the other diets, the results showed that the carcass values (parameters) of broilers fed 100% pearl millet were similar or non-superior to the control group and the other diets. Similarly, blood parameters were not significantly different between groups. These results show that pearl millet supplemented with enzyme can replace maize in broiler diets without affecting carcass yields and haematological parameters.

Effect of Fibrinolytic Microbes and Enzymes on Biochemical Blood Parameters in Crossbred Calves

2019 ◽  
Vol 15 (02) ◽  
pp. 18-21
Author(s):  
R I Kapadiya ◽  
S V Shah ◽  
Y G Patel ◽  
P R Pandya
Keyword(s):  
Blood Parameters ◽  
The Other ◽  
Control Group ◽  
Glutamic Pyruvic Transaminase ◽  
Normal Range ◽  
Blood Samples ◽  
Overall Performance ◽  
Similar Body ◽  
Control Plasma ◽  
Plasma Phosphorus

Twenty crossbred (HF x Kankrej) calves were allotted randomly to five groups (each had one male and three female calves) almost with similar body weight (85.70±6.37 kg) and age (167.55±21.70 days). Experimental calves of the control group were offered hybrid napier untreated, and the other four groups were offered hybrid napier treated with (i) fungus- Aspergillus spp. (1×107 per g feed), (ii) fibrolytic bacteria- Escherichia spp. (106 CFU per g feed), (iii) xylanase (50 mL/kg having xylanase 1.2 IU/mL), and (iv) consortium of 1/3rd dose of fungus + bacteria + enzyme as treatments for 140 days. The blood samples were collected from each calf at a biweekly intervals in the EDTA vacuttee. Nutrients offered to crossbred calves were sufficient to satisfy the nutrients requirements. Bacteria fed calves had significantly higher (plessthan0.05) mean hemoglobin levels than control. Blood plasma glucose of calves in fibrolytic microbes and enzyme groups was statistically similar to control. Feeding of fungus, bacteria, enzyme, and consortium was without a significant effect on plasma total proteins.In comparison to control (7.53 mg/dL), the plasma phosphorus was significantly (plessthan0 0.05) higher in calves fed enzyme (7.87 ± 0.20 mg/dl) and lower in group fed fungus (7.13 mg/dL). In contrast, in bacteria (7.57 mg/dL) and consortium group (7.60 mg/dL) it was statistically similar to control. Plasma glutamic pyruvic transaminase concentration of crossbred calves was lower ( pless than 0.05) in enzyme and higher (p lessthan 0.05) in fungus, bacteria, and consortium groups than in control, while plasma glutamic oxaloacetate transaminase concentration in all groups was statistically similar. In conclusion, all blood parameters of different groups were within normal range and without adverse effect on the overall performance of calves.

X-IRRADIATION OF RIBOSE AND DEOXYRIBOSE DERIVATIVES

1962 ◽  
Vol 40 (11) ◽  
pp. 1553-1565 ◽  
Author(s):  
D. K. Myers
Keyword(s):  
Aqueous Solution ◽  
Nucleic Acids ◽  
The Other ◽  
Free Sugar ◽  
Blood Samples ◽  
Other Hand ◽  
Dilute Aqueous Solution ◽  
X Irradiation ◽  
In Cells

During X-irradiation of ribosides and deoxyribosides in dilute aqueous solution, purine and sugar moieties were destroyed at approximately the same rate. Pyrimidine moieties, on the other hand, were destroyed more rapidly, resulting in a fairly rapid liberation of free sugar during irradiation of pyrimidine ribosides or deoxyribosides. No marked differences in the rates of destruction of ribose and deoxyribose derivatives were observed in these experiments.Similar reactions were observed during irradiation of blood samples. However, acid-soluble ribonucleotides in the blood proved to be much more sensitive than the nucleic acids or proteins to destruction by X-radiation. The significance of these findings is discussed with relation to the damage produced in cells by X-irradiation.

Percent carboxyhemoglobin in resting humans exposed repeatedly to 1,500 and 7,500 ppm CO

1987 ◽  
Vol 63 (2) ◽  
pp. 820-827 ◽  
Author(s):  
P. Tikuisis ◽  
F. Buick ◽  
D. M. Kane
Keyword(s):  
Theoretical Model ◽  
Venous Blood ◽  
Alveolar Ventilation ◽  
The Other ◽  
Predictive Capability ◽  
Blood Samples ◽  
Nominal Dose ◽  
Significant Difference ◽  
Co Concentration ◽  
Respiratory Gases

Eleven nonsmoking male resting subjects were exposed to two transient CO profiles to examine whether the resultant carboxyhemoglobin (HbCO) differs with CO concentration for a fixed total CO dose and to determine the predictive capability of the theoretical model of Coburn et al. (J. Clin. Invest. 44: 1899–1910, 1965) using measured alveolar ventilation. One profile consisted of five sequential exposures to 1,500 ppm CO for 5 min each and spaced 3 min apart. The other consisted of five sequential exposures to 7,500 ppm CO for 1 min each and spaced 7 min apart. The subjects, therefore, were exposed to the same overall nominal dose of 37,500 ppm.min. During the experiment, the subject's ventilatory functions and respiratory gases were recorded continuously, and the resultant HbCO% was measured in venous blood samples by gas chromatography. Mean increase (+/- SD) in HbCO% per exposure was 2.08 +/- 0.27% for the 1,500 ppm CO exposures and 2.05 +/- 0.29% for the 7,500 ppm CO exposures with no significant difference between the two. When the measured values of the subject's alveolar ventilation were applied to the theoretical model of Coburn et al., the predicted rate of HbCO% formation was found to agree with the experimental results.

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