Interaction of unsaturated fatty acids with rat liver glucocorticoid receptors: studies to localize the site of interaction

1993 ◽  
Vol 129 (4) ◽  
pp. 348-355 ◽  
Author(s):  
Charlotte Sumida ◽  
Geneviève Vallette ◽  
Emmanuel A Nunez
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Polyunsaturated Fatty Acids ◽  
Rat Liver ◽  
Glucocorticoid Receptors ◽  
Unsaturated Fatty Acids ◽  
Competitive Inhibition ◽  
Binding Domain ◽  
Hormone Binding

Polyunsaturated fatty acids have been shown to decrease the binding of [3H]dexamethasone to rat liver glucocorticoid receptors by mixed non-competitive inhibition, suggesting that these fatty acids interact at a site on the receptor different from the hormone binding site. The present study was undertaken to localize the site of interaction of polyunsaturated fatty acids on the receptor by comparing the differential effects of docosahexaenoic acid (a 22-carbon polyunsaturated fatty acid of the series n −3) on antagonist (RU486) and agonist binding, by covalent cross-linking of the hsp 90 and other proteins to the receptor to attempt to mask the site of interaction, by limited trypsinization to cleave the site and by using antibodies against specific epitopes to prevent fatty acid access by steric hindrance. Binding [3H]RU486 was not inhibited by docosahexaenoic acid at a concentration (60 μmol/l) that increases the dissociation constant of [3H]dexamethasone eightfold. Covalent stabilization of the hetero-oligomeric glucocorticoid receptor structure did not keep the fatty acid from inhibiting [3H]dexamethasone binding. The binding to the receptor of monoclonal and polyclonal antibodies against different domains of the receptor did not sterically hinder the fatty acid interaction with the receptor. After limited trypsinization of the receptor, the fatty acid still increased the dissociation rate constant of [3H]dexamethasone binding, indicating that the site of interaction of polyunsaturated fatty acids is on a fragment of the receptor containing the hormone-binding domain and some sequences C-terminal of the DNA-binding domain.

Free fatty acid level in rat liver after ingestion of polyunsaturated fatty acids

Lipids ◽  
1969 ◽  
Vol 4 (1) ◽  
pp. 77-79 ◽  
Author(s):  
Akira Yamamoto ◽  
Masahiro Isozaki ◽  
Takeshi Ishibe ◽  
Mitsuo Nishikawa
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Rat Liver ◽  
Acid Level ◽  
Free Fatty Acid Level ◽  
Fatty Acid Level

scholarly journals Oil yield and fatty acid profile of seeds of three Salvia species. A comparative study

2015 ◽  
Vol 61 (2) ◽  
pp. 14-29 ◽  
Author(s):  
Mouna Ben Farhat ◽  
Rym Chaouch -Hamada ◽  
Ahmed Landoulsi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Comparative Study ◽  
Seed Oil ◽  
Unsaturated Fatty Acids ◽  
Fatty Acid Content ◽  
Fatty Acid Analysis ◽  
Major Fatty Acid ◽  
Oil Yield

Summary A comparative study of the oil yield and fatty acid composition of three Salvia species seeds collected in different locations has been conducted. Seed oil extraction was made using a Soxhlet-extractor and fatty acid analysis was undertaken using a GC-FID. The effect of the collecting site on oil yield, as well as the content of individual fatty acid and total fatty acid and fatty acid content was significant. Seed oil yield varied from 14.94 to 22.83% and the total fatty acids ranged from 67.36 to 82.49 mg/g DW. α-Linolenic (24.02-49.19%), linoleic (20.13-42.88%), oleic (12.97-17.81%) and palmitic (8.37-16.63%) acids were the most abundant fatty acids in all analyzed samples. α-Linolenic acid was found to be the major fatty acid in S. verbenaca and S. officinalis species, however, S. aegyptiaca was characterized by the prevalence of linoleic acid. Among the unsaturated fatty acids, which were represented in all samples in high amounts (78.16-89.34%), the polyunsaturated fatty acids (α-linolenic and linoleic acids) showed important levels ranging from 63.09 to 74.71%. Seeds of S. verbenaca were the richest in polyunsaturated fatty acids.

scholarly journals Docosahexaenoic Acid and Eicosapentaenoic Acid Intakes Modulate the Association of FADS2 Gene Polymorphism rs526126 with Plasma Free Docosahexaenoic Acid Levels in Overweight Children

2021 ◽  
Vol 11 (21) ◽  
pp. 9845
Author(s):  
Alexandra Mihailescu ◽  
Vlad Serafim ◽  
Corina Paul ◽  
Nicoleta Andreescu ◽  
Diana-Andreea Tiugan ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Gene Polymorphism ◽  
Polyunsaturated Fatty Acids ◽  
Genetic Variants ◽  
The Body ◽  
Conversion Rates ◽  
Custom Made ◽  
The Impact

Polyunsaturated fatty acids are involved in a wide variety of biological functions. Linoleic acid and alpha-linolenic acid are two essential fatty acids that the body cannot synthesize. The conversion rates in the body depend on FADS2 genetic variants. Certain variations in this gene are directly responsible for the low levels and poor conversion efficiency of the delta-6 desaturase enzyme, resulting in low circulating levels of docosahexaenoic acid. In this study, we evaluated the impact of the rs526126 FADS2 gene polymorphism on fatty acid levels in a group of two hundred children (n = 95 males, n = 105 females) aged 7–18 years, with obesity defined by BMI > +2 SD. Fatty acid quantification was performed by LC-MS/MS while genotyping for genetic variants was performed using a custom-made hotspot sequencing panel of 55 SNPs. Our results suggest that rs526126 FADS2 gene polymorphism specifically impacts the plasma levels of free n-3 polyunsaturated fatty acids. Finally, the presence of the minor allele G of rs526126 could have beneficial effects, as it was associated with higher levels of free docosahexaenoic acid in plasma, especially in children with low n-3 intakes.

Determination of Fatty Acid Composition, Cholesterols, Triglyceride and Vitamin Contents of Some Selected Fishes from Assam, India

2020 ◽  
Vol 16 (2) ◽  
pp. 213-219
Author(s):  
Arjina Parbin Sarkar ◽  
Sanjay Basumatary ◽  
Santanu Sarma ◽  
Sandeep Das
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Polyunsaturated Fatty Acids ◽  
Eicosapentaenoic Acid ◽  
Acid Composition ◽  
Fish Species ◽  
The Body ◽  
Lipid Components

Background: Fishes are good sources of the fatty acids such as ω-3 and ω-6 polyunsaturated fatty acids, and fat-soluble vitamins for human consumption which play vital roles for various biological processes in the body and help in the proper growth and prevention of diseases. Objective: The objective of the present study was to determine the fatty acid composition, cholesterols, triglyceride and vitamin contents of some selected fishes from Hel river, Assam, India. Methods: Fatty acid composition of fish species was examined using gas chromatography-mass spectrometry, lipid components were determined following the reported methods and vitamins A and D contents were investigated by reversed-phase high-performance liquid chromatography. Results: Fatty acid compositions varied from 51.20-89.47% of saturated fatty acids, 0.27-19.68% of monounsaturated fatty acids and 1.75-30.76% of polyunsaturated fatty acids. Eicosapentaenoic acid and docosahexaenoic acid ranged from 0.54-22.30% and 1.26-18.85%, respectively. The fish species showed varying amounts of lipid components. The vitamins A and D were found in the range of 15.85-1287.0 μg/100 g and 45.0-677.24 μg/100 g, respectively. Conclusion: The fish species of this study are found rich in ω-3 polyunsaturated fatty acids such as eicosapentaenoic acid and docosahexaenoic acid along with noticeable amounts of vitamins A and D. Hence, these fish species have the potentials to serve as the natural dietary supplements for ω-3 fatty acids and other nutrients.

scholarly journals Effects of high-fat diets on hepatic fatty acid oxidation in the rat Isolation of rat liver peroxisomes by vertical-rotor centrifugation by using a self-generated, iso-osmotic, Percoll gradient

1981 ◽  
Vol 196 (1) ◽  
pp. 149-159 ◽  
Author(s):  
C E Neat ◽  
M S Thomassen ◽  
H Osmundsen
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Rat Liver ◽  
Unsaturated Fatty Acids ◽  
High Fat ◽  
Beta Oxidation ◽  
Vertical Rotor ◽  
High Fat Diets ◽  
Fat Diets ◽  
Long Chain

1. Rat liver peroxisomal fractions were isolated in iso-osmotic Percoll gradients by using vertical-rotor centrifugation. The fractions obtained with rats given various dietary treatments were characterized. 2. The effect on peroxisomal beta-oxidation of feeding 15% by wt. of dietary fat for 3 weeks was investigated. High-fat diets caused induction of peroxisomal beta-oxidation, but diets rich in very-long-chain mono-unsaturated fatty acids produced a more marked induction. 3. Peroxisomal beta-oxidation induced by diets rich in very-long-chain mono-unsaturated fatty acids can oxidize such acids. Trans-isomers of mono-unsaturated fatty acids are oxidized at rates that are faster than, or similar to, those obtained with corresponding cis-isomers. 4. Rates of oxidation of [14-14C]erucic acid by isolated rat hepatocytes isolated from rats fed on high-fat diets increased with the time on those diets in a fashion very similar to that previously reported for peroxisomal beta-oxidation [see Neat, Thomassen & Osmundsen (1980) Biochem, J. 186, 369-371]. 5. Total liver capacities for peroxisomal beta-oxidation (expressed as acetyl groups produced per min) were estimated to range from 10 to 30% of mitochondrial capacities, depending on dietary treatment and fatty acid substrate. A role is proposed for peroxisomal beta-oxidation in relation to the metabolism of fatty acids that are poorly oxidized by mitochondrial beta-oxidation, and, in general, as regards oxidation of fatty acids during periods of sustained high hepatic influx of fatty acids.

BLOOD FATTY ACID COMPOSITION IN COWS DEPENDING ON THE TYPE OF AUTONOMIC REGULATION IN SUMMER PERIOD

2021 ◽  
Vol 12 (4) ◽  
Author(s):  
I. A. Hryshchuk ◽  
◽  
V. I. Karpovskyi ◽  
V. V. Danchuk ◽  
B. V. Gutyj ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Blood Plasma ◽  
Unsaturated Fatty Acids ◽  
Physiological Activity ◽  
Saturated Fatty Acids ◽  
Nervous Activity ◽  
Autonomic Regulation ◽  
In Blood Plasma

Coordination of physiological activity and intensity of metabolism in various organs and tissues of a productive animal, depending on environmental conditions and own needs, is provided by the regulatory activity of the corresponding nerve centers. However, the features of the autonomic regulation of the nervous system in this matter can have a significant impact not only on the physiological activity of animals but also on their productivity. Therefore, the question of studying the influence of autonomic regulation on the animal body in general and the interaction of the autonomic system and lipid metabolism is quite relevant. Groups of animals were formed by determining the state of the cardiovascular system according to Baevskyi. Blood plasma was used for the study, lipid extraction was performed by the Folch method. Fatty acid analysis was performed on a Trace GC Ultra gas chromatograph (USA) with a flame ionization detector. Studies have shown that the relative content of saturated fatty acids in blood of normotonics was the highest compared with other groups: sympathotonics – by 1.9%; vagotonics – 0.48%. Regarding the concentration of saturated fatty acids in sympathotonics, it should be noted that the content of stearic acid was the highest (18.07 ± 0.01; P < 0.001) and saturated fatty acids from C6 to C16 were characterized by the lowest values (P < 0.01–0.05) in comparison with other groups The total content of unsaturated fatty acids in blood plasma of sympathotonics and vagotonics was 1.19% and 0.49% higher, respectively, compared with normotonics. Quite interesting is the fact that sympathotonics were characterized by the highest content of polyunsaturated fatty acids, the content of which decreased in the range C18:2n6 > C20:4n6 > C18:3n3 > C22:6n3 > C22:5n3, a similar sequence we observed in other groups, although the relative concentration of acids could fluctuate. Normotonics were characterized by the highest levels of some polyunsaturated fatty acids (C18:3n3, C22:5n3, C22:6n3) (P < 0.05-0.01), while levels of some unsaturated fatty acids (C18: 1n9, C20: 3n6) were the lowest. Thus, the type of nervous activity has a significant effect on the ratio of fatty acids in blood plasma of cows and autonomous regulation affects the metabolic processes in the animal’s body.

Modulatory Effects of Unsaturated Fatty Acids on the Binding of Glucocorticoids to Rat Liver Glucocorticoid Receptors*

Endocrinology ◽  
1991 ◽  
Vol 129 (3) ◽  
pp. 1363-1369 ◽  
Author(s):  
GENEVIÉVE VALLETTE ◽  
ANNE VANET ◽  
CHARLOTTE SUMIDA ◽  
EMMANUEL A. NUNEZ
Keyword(s):  
Fatty Acids ◽  
Rat Liver ◽  
Glucocorticoid Receptors ◽  
Unsaturated Fatty Acids
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