scholarly journals Long-term storage of mouse spermatozoa after evaporative drying

Reproduction ◽  
2007 ◽  
Vol 133 (5) ◽  
pp. 919-929 ◽  
Author(s):  
Ming W Li ◽  
John D Biggers ◽  
Heidi Y Elmoazzen ◽  
Mehmet Toner ◽  
Lynda McGinnis ◽  
...  
Keyword(s):  
Storage Temperature ◽  
Blastocyst Formation ◽  
Nitrogen Gas ◽  
Transfer Rates ◽  
Fertilization Rates ◽  
Long Term Storage ◽  
First Time ◽  
Term Storage ◽  
Better Than

To determine if mouse spermatozoa could be preserved long-term without using liquid nitrogen, mouse spermatozoa in trehalose–EGTA solution were partially evaporatively dried under nitrogen gas (5 min at flow rate10 l/min) and stored for 1 week and 5 months at 4, −20, and −80 °C before intracytoplasmic sperm injection. Fertilization rates were neither different with spermatozoa stored at 4, −20, or −80 °C for 1 week or 1, 3, and 5 months respectively, nor blastocyst formation rates with spermatozoa stored for 1 week and 1 month. However, spermatozoa stored at 4 and −20 °C for 3 months resulted in fewer blastocysts (35.1 and 54.3% respectively) when compared with spermatozoa stored at −80 °C (74.4%). Blastocyst formation rates using spermatozoa stored for 5 months at −20 °C (57.4%) or −80 °C (74.5%) were not significantly different from those stored for 3 months at the same temperatures respectively, but were significantly better than those stored for 5 months at 4 °C (10.2%). Blastocysts derived from spermatozoa stored for 3 and 5 months at −20 and −80 °C respectively, were then transferred to pseudopregnant mothers to develop into healthy liveborn offspring. No significant differences were found in embryo transfer rates (number of pups born/number of embryos transferred), weaning rates, or sex ratios of resultant pups, which were healthy and reproductively sound. These results demonstrate for the first time that partially evaporatively dried mouse spermatozoa in trehalose–EGTA solution can be preserved for long term at −20 and −80 °C. The possibility that the storage temperature must be less than the glass transition temperature is discussed.

scholarly journals Scientific support for innovative development of wheat bread long-term storage production

2020 ◽  
Vol 176 ◽  
pp. 03021
Author(s):  
Natalia Zavorokhina ◽  
Natalia Pankratyeva ◽  
Nadezhda Goncharova
Keyword(s):  
Shelf Life ◽  
Fatty Acid Esters ◽  
Wheat Bread ◽  
Innovative Development ◽  
Long Term Storage ◽  
High Degree ◽  
First Time ◽  
Term Storage ◽  
Acid Esters

Nowadays in the context of innovative development the bread intended for long-term storage is necessary in hard-to-reach areas of Sverdlovsk region, participants of expeditions, workers of logging, geological parties, etc. Taking into account the constant demand for such products, it is necessary to develop new approaches to their creation.The aim of the study was the development of long-term storage bread from wheat flour, preserving consumer characteristics throughout the shelf life.The synergism of betulin,nisin and ascorbic acid was studied for the first time when they are used together to inhibit potato bread disease (hereinafter CBH). It is proved that the use of additives, including 0.015% nisin together with 0.005% betulin to the mass of flour increases the shelf life of wheat bread up to 7 days since betulin inhibits CBH, nisin inhibits the development of mold under pH conditions in the range of 5.5-6.5.For the first time, a technique is proposed for producing betulin nanosuspension with a high degree of dispersion. The effect of betulin nanosuspension with emulsifiers of calcium lactylate, polyglycerol fatty acid esters, lecithin on the inhibition of CBH and an increase in the shelf life of wheat bread was studied.

Self-Irradiation of Monazite Ceramics: Contrasting Behavior of PuPO4 and (La,Pu)PO4 Doped with Pu-238

2004 ◽  
Vol 824 ◽  
Author(s):  
Boris E. Burakov ◽  
Maria A. Yagovkina ◽  
Vladimir M. Garbuzov ◽  
Alexander A. Kitsay ◽  
Vladimir A. Zirlin
Keyword(s):  
Radiation Damage ◽  
Crack Formation ◽  
Alpha Decay ◽  
Xrd Analysis ◽  
X Ray Diffraction ◽  
Deep Blue ◽  
Long Term Storage ◽  
First Time ◽  
Term Storage

AbstractTo investigate the behavior of monazite during accelerated radiation damage, which simulates effects of long term storage, 238Pu-doped polycrystalline samples of (La,Pu)PO4 and PuPO4 were synthesized for the first time ever and studied using powder X-ray diffraction (XRD) analysis and optical microscopy. The starting precursor materials were obtained by precipitation of La and (or) Pu from their aqueous nitrate solutions followed by calcination in air at 700°C for 1 hour, cold pressing, and sintering in air at 1200-1250°C for 2 hours. The 238Pu contents in ceramic samples measured using gamma spectrometry were (in wt.% el.): 8.1 for (La,Pu)PO4 and 7.2 for PuPO4. The (La,Pu)PO4 monazite remained crystalline at ambient temperature up to a cumulative dose of 1.19 × 1025 alpha decays/m3. In contrast, the PuPO4 monazite became nearly completely amorphous at a relatively low dose of 4.2 × 1024 alpha decays/m3. Swelling and crack formation due to the alpha decay damage was observed in the PuPO4 ceramic. Also, under self-irradiation this sample completely changed color from initial deep blue to black. The (La,Pu)PO4 monazite was characterized by a similar change in color from initial light blue to gray, however, no swelling or crack formation have so far been observed. The results of this study allow us to conclude that the radiation damage behavior of monazite strictly depends on the chemical composition. The justification of monazite-based ceramics as actinide waste forms requires additional investigation.

Combined effects of storage temperature variation and dynamic controlled atmosphere after long-term storage of ‘Maxi Gala’ apples

2022 ◽  
Vol 31 ◽  
pp. 100770
Author(s):  
Lucas Mallmann Wendt ◽  
Vagner Ludwig ◽  
Fabiane Portella Rossato ◽  
Magno Roberto Pasquetti Berghetti ◽  
Erani Eliseu Schultz ◽  
...  
Keyword(s):  
Temperature Variation ◽  
Storage Temperature ◽  
Combined Effects ◽  
Controlled Atmosphere ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Storage Temperature, Controlled Atmosphere, and 1-Methylcyclopropene Effects on α-Farnesene, Conjugated Trienols, and Peroxidation in Relation with Superficial Scald, Pithy Brown Core, and Fruit Quality of ‘d’Anjou’ Pears during Long-term Storage

2016 ◽  
Vol 141 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Yan Wang
Keyword(s):  
Storage Temperature ◽  
Storage Conditions ◽  
Controlled Atmosphere ◽  
Metabolic Balance ◽  
Superficial Scald ◽  
Long Term Storage ◽  
Temperature Controlled ◽  
Term Storage

Alternatives to ethoxyquin (Etq) are needed for controlling superficial scald of ‘Anjou’ european pears (Pyrus communis) during long-term storage. The current commercial standard storage conditions [Etq + −1 °C + controlled atmosphere (CA) with 1.5 kPa O2] reduced scald occurrence compared with control fruit (−1 °C + CA) during 6–8 months storage. At 1 °C in air, 1-methylcyclopropene (1-MCP) fumigation at 0.15 µL·L−1 at harvest was more efficient on reducing scald than Etq but did not prevent scald during 6–8 months storage. The 1-MCP-treated fruit at 1 °C in air developed their ripening capacity at 20 °C following 6–8 months storage but had deceased shipping ability (softening and yellowing of fruit). Although Etq inhibition of scald was associated with the inhibition of α-farnesene oxidation to conjugated trienols (CTols); 1-MCP reduced α-farnesene synthesis and thereby the availability of substrate to oxidize to CTols. CA storage at 1.5 kPa O2 totally prevented scald and retarded the loss of shipping ability without affecting the ripening capacity of 1-MCP-treated fruit at 1 °C through further decreases in the syntheses of ethylene, α-farnesene and CTols during 6–8 months storage. In addition, 1-MCP prevented a CA-induced disorder, pithy brown core (PBC), in ‘Anjou’ pears possibly through enhancing an oxidative/reductive metabolic balance during extended storage. In conclusion, the combinations of 1 °C + 1-MCP + CA is a potential commercial alternative to Etq for scald control while allowing the 1-MCP-treated ‘Anjou’ pears to recover ripening capacity during the shelf life period after 6–8 months storage.

scholarly journals Chemical and Biological Stability of Indole-3-Butyric Acid (IBA) After Long-term Storage at Selected Temperatures and Light Regimes

1988 ◽  
Vol 6 (2) ◽  
pp. 39-41 ◽  
Author(s):  
James A. Robbins ◽  
Mark J. Campidonica ◽  
David W. Burger
Keyword(s):  
Butyric Acid ◽  
Storage Temperature ◽  
Naphthaleneacetic Acid ◽  
Light Regimes ◽  
Long Term Storage ◽  
Color Development ◽  
Clear Glass ◽  
Indole 3 Acetic Acid ◽  
Term Storage

Concentrated [4.9 mM (1,000 ppm) and 24.6 mM (5,000 ppm)] IBA solutions in 50% isopropyl alcohol were stored in amber and clear glass bottles at 3 temperatures [22–25°, 6°, O°C (72–77°, 43°, 32°F)]. No significant change in biological activity of the solutions or breakdown of IBA was observed for solutions stored for 4 and 6 months. Solution color changed during storage. Color development was dependant on storage temperature, but not on exposure to light. Chemical names used: IAA = indole-3-acetic acid; IBA = indole-3-butyric acid; NAA = 1-naphthaleneacetic acid

scholarly journals Storage and Viability Testing of Protea Pollen

1996 ◽  
Vol 121 (5) ◽  
pp. 804-809 ◽  
Author(s):  
I. David van der Walt ◽  
Gail M. Littlejohn
Keyword(s):  
Storage Temperature ◽  
Pollen Viability ◽  
Germination Percentage ◽  
Long Term Storage ◽  
Fresh Pollen ◽  
The Relationship ◽  
Term Storage ◽  
Storage Temperatures

The influence of storage temperature and humidity on pollen viability was studied in four Protea species. Pollen was stored at a range of temperatures and relative humidities for up to 1 year and tested for ability to germinate in vitro. Pollen of P. repens (L.) L. `Sneyd', P. eximia (Salisb. ex Knight) Fourcade `Fiery Duchess' and P. magnifica Link. clone T 84 07 05 stored at -196 °C and -14 to -18 °C retained a germination percentage as high as that of fresh pollen regardless of humidity. Humidity control became increasingly important at storage temperatures above 0 °C. The study showed that long-term storage of Protea pollen is not feasible at temperatures above 0 °C. The relationship between germinability and fluorochromasia (FCR) was studied during storage of `Sneyd' pollen. The correlations between FCR and germinability were found to be low and nonsignificant. Fifteen-month-old cryopreserved `Sneyd' pollen functioned in fertilization and seed set as effectively as fresh pollen.

Efficacy of the Antigenicity-Retaining Ability of Fixative Solutions for Liquid-Based Cytology: Immunocytochemistry of Long-Term Storage

2021 ◽  
pp. 1-12
Author(s):  
Hiaki Sato ◽  
Yoshiaki Norimatsu ◽  
Satoshi Irino ◽  
Takeshi Nishikawa
Keyword(s):  
Cell Membrane ◽  
Room Temperature ◽  
Storage Temperature ◽  
Storage Period ◽  
Storage Conditions ◽  
Immunocytochemical Staining ◽  
Long Term Storage ◽  
Liquid Based Cytology ◽  
Term Storage

<b><i>Introduction/Objective:</i></b> Liquid-based cytology (LBC) is advantageous as multiple stained specimens can be prepared and used for additional assays such as immunocytochemical and molecular-pathological investigations. Two types of preservative-fixative solutions (fixatives) are used for nongynecologic specimens used in the BD SurePath-LBC (SP-LBC) method, and their components vary. However, few studies have evaluated the differences in antigen-retaining ability between these fixatives. Therefore, we investigated and compared the antigen-retaining ability of the fixatives in immunocytochemical staining (ICC) under long-term storage conditions. <b><i>Materials and Methods:</i></b> Sediments of cultured RAJI cells (derived from Burkitt’s lymphoma) were added to each fixative (red and blue) and stored at room temperature for a specified period (1 h; 1 week; and 1, 3, and 6 months). The specimens were then prepared using the SP-LBC method and subjected to ICC. Positivity rate was calculated using the specimens fixed at room temperature for 1 h as a control. Antibodies against Ki67 expressed in the nucleus and against CD20 and leukocyte common antigen (LCA) expressed on the cell membrane were used. <b><i>Results:</i></b> For CD20 and LCA, the positivity rate increased with time in the red fixative compared with that in the control. In the blue fixative, the positivity rate was highest at 1 h and was maintained at a high level throughout the storage period. In contrast, the Ki67 positivity rate was highest at 1 h in both red and blue fixatives and markedly decreased with time. Therefore, although refrigerated (8°C) storage was used, no improvement was noted. <b><i>Conclusions:</i></b> Long-term storage is possible for cell membrane antigens at room temperature; however, it is unsuitable for intranuclear antigens. Therefore, we conclude that suitable fixative type and storage temperature differ based on antigen location. Further investigation is warranted.

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