scholarly journals Changes in sperm quality and lipid composition during cryopreservation of boar semen

Reproduction ◽  
2001 ◽  
pp. 395-401 ◽  
Author(s):  
S Cerolini ◽  
A Maldjian ◽  
F Pizzi ◽  
TM Gliozzi
Keyword(s):  
Lipid Content ◽  
Free Cholesterol ◽  
Sperm Quality ◽  
Cholesterol Content ◽  
Sperm Viability ◽  
Freezing And Thawing ◽  
Motile Cells ◽  
Boar Semen ◽  
Thawing Procedure ◽  
Fresh Semen

The effect of cryopreservation on boar sperm viability, motility, lipid content and antioxidant enzymatic activities was studied. Three classes of semen were determined according to a cluster analysis on the basis of the proportion of live and dead cells after freezing and thawing. The classes identified were: high (H, n = 4), average (A, n = 12) and low (L, n = 3) viability. The concentration of sperm cells decreased from class H to A to L. Fresh semen samples with higher viability and a higher proportion of motile cells also maintained better quality after the freezing and thawing procedure. Sperm viability and motility in both fresh and thawed samples were similar in classes H and A, while significantly lower values were measured in class L. The relative decrease in sperm viability and motility after cryopreservation increased from class H to A to L. The lipid content of spermatozoa (micrograms per 10(9) cells) increased significantly after freezing and thawing in classes H and A but not in class L. This result indicated that active sperm lipid metabolism might be responsible for the increase in lipid content. Phospholipid and triacylglycerol contents increased whereas free cholesterol content decreased after thawing. The fatty acid composition of fresh spermatozoa was similar in all three classes. The proportion of polyunsaturated fatty acids decreased significantly after freezing and thawing, indicating contamination from the diluent or peroxidation. After freezing and thawing, superoxide dismutase activity in spermatozoa was significantly higher in class L than in classes H and A, which did not differ from each other.

scholarly journals Macro- and microelements in serum and seminal plasma as biomarkers for bull sperm cryotolerance

2021 ◽  
Vol 63 (1) ◽  
Author(s):  
Maja Zakošek Pipan ◽  
Petra Zrimšek ◽  
Breda Jakovac Strajn ◽  
Katarina Pavšič Vrtač ◽  
Tanja Knific ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Quality Characteristics ◽  
Freezing And Thawing ◽  
Additional Tool ◽  
Bull Semen ◽  
Bull Sperm ◽  
Fresh Semen

ABSTRACT Background Wide variation in fertility rates is observed when using frozen bull semen, even when the bulls have met quality standards for semen production. Therefore, a simple and reliable test to assess the freezing potential of bull semen based on the analysis of fresh semen or blood would be of great value. Attention is now turning to assessment of seminal plasma components such as proteins and elements. In the present study, the concentrations of macro- and microelements in fresh bull semen plasma and in serum and their correlation with quality characteristics of fresh semen and with semen quality after freezing and thawing were determined. Ejaculates were collected from 30 mature bulls, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability and DNA fragmentation were determined on the day of collection and after freezing and thawing. The concentrations of macroelements (Na, Mg, K and Ca) and microelements (Cu, Fe, Zn and Se) were determined in the seminal plasma and serum. The semen samples were classified into satisfactory and unsatisfactory groups according to the fresh semen quality. Results Zinc and Se levels measured in serum were associated with almost all fresh and frozen-thawed semen quality characteristics, while Fe levels were associated only with acrosomal defects in fresh semen. Zinc and Fe levels in fresh seminal plasma were associated with various quality characteristics of fresh and frozen-thawed semen, while Se level in fresh seminal plasma was not associated with any of the semen quality characteristics. Conclusions Microelements were shown to be useful as biomarkers involved in the analysis of bull sperm quality and could be used as an additional tool to predict bull semen quality after freezing and thawing. Our results confirm that the analysis of Zn and Se levels in serum and Zn, Cu and Fe levels in fresh seminal plasma can provide information to discriminate between bull semen samples with spermatozoa with high or low cryotolerance.

scholarly journals O papel do dimetilsulfóxido na criopreservação de sêmen de Curimba (Prochilodus lineatus)

2015 ◽  
Vol 36 (5) ◽  
pp. 3471
Author(s):  
Antonio Sergio Varela Junior ◽  
Estela Fernandes Silva ◽  
Tainã Figueiredo Cardoso ◽  
Érica Yokoyama Namba ◽  
Rodrigo Desessards Jardim ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Membrane Integrity ◽  
Fertilization Rate ◽  
Dna Integrity ◽  
Hatching Rate ◽  
Sperm Viability ◽  
Prochilodus Lineatus ◽  
Dmso Concentration ◽  
Fresh Semen

<p class="Pa7">Cryopreservation of Curimba semen (Prochilodus lineatus) is ecological and commercial importance. The objective of this study was to evaluate the effect of different concentrations (2, 5, 8 and 11%) of dimethyl sulfoxide (DMSO) diluted in Betsville Thawing Solution (BTS) on the quality of post-thaw semen Curimba. We analyzed the rate and period motility, sperm viability, membrane integrity and DNA, mitochondrial functionality, and fertilization and hatching rate. The plasma membrane and DNA integrity of a DMSO concentration of 11% obtained better results than the concentration of 5% (p &lt;0.05). However, treatment of 5% DMSO resulted in a longer latency and a higher fertilization rate and hatching, in other sperm quality equal to that of fresh semen. The results of this study indicate that 5% DMSO is ideal for cryopreservation of semen Curimba.</p>

scholarly journals Relevance of the Ejaculate Fraction and Dilution Method on Boar Sperm Quality during Processing and Conservation of Seminal Doses

2021 ◽  
Vol 8 (12) ◽  
pp. 292
Author(s):  
Blanca Sebastián-Abad ◽  
Pedro José Llamas-López ◽  
Francisco Alberto García-Vázquez
Keyword(s):  
Sperm Quality ◽  
Resistance Test ◽  
Dilution Method ◽  
Sperm Viability ◽  
The Real ◽  
Progressive Motility ◽  
Semen Collection ◽  
Transport Effect ◽  
Boar Semen ◽  
Semen Processing

During boar semen processing and distribution, maximizing the work protocols in the laboratories becomes essential for the conservation of seminal doses. One of the recent implementations in the boar studs to improve efficiency has been semi-automatic semen collection systems, which do not allow to discard fractions of the ejaculate. The objective of this work was to evaluate the dilution method and vibrations (simulating delivery transport) effect on sperm quality (motility, viability, morphology, thermo-resistance test) according to the fraction of ejaculate collected. Two different fractions of the ejaculate were obtained [rich fraction (RF); total fractions (TF)] from six boars, and two dilution methods applied [pouring the extender over the semen (control; ES); pouring the semen over the extender (reverse; SE)]. The seminal doses (2000 × 106 sperm/50 mL) were preserved for 5 days. The results showed that the fraction collected affects sperm quality (better total and progressive motility, and faster sperm in TF; p < 0.05) regardless of the dilution method applied. However, these differences diminished after submitting the semen to the thermo-resistance test, with only differences in sperm viability being observed (p < 0.05). When seminal doses were subjected to vibrations, the sperm viability was more affected in the TF than in the RF group (p < 0.05). In conclusion, using the TF ejaculate leads to comparable results to the RF in sperm quality during storage regardless of the dilution method applied. However, the vibrations of seminal doses are more affected in doses prepared with TF than with RF, although more factors should be included to approach the real conditions during transport.

BOAR, BREED AND ENVIRONMENTAL EFFECTS ON MOTILITY OF FROZEN-THAWED SPERMATOZOA

1986 ◽  
Vol 66 (3) ◽  
pp. 663-668 ◽  
Author(s):  
S. M. JOYAL ◽  
B. W. KENNEDY ◽  
J. N. WILKINS
Keyword(s):  
Key Words ◽  
Environmental Effects ◽  
Current Practice ◽  
Volume Concentration ◽  
Motile Sperm ◽  
Freezing And Thawing ◽  
Frozen Semen ◽  
Boar Semen ◽  
Fresh Semen

A total of 5186 ejaculates from 115 boars of five breeds (48 Yorkshire, 25 Landrace, 20 Duroc, 18 Hampshire and four Lacombe) were collected and frozen between 1975 and 1980. Frozen semen was thawed and evaluated for percentage progressive motile sperm (post-thaw rating) and loss in percentage motile sperm due to freezing and thawing. Boar repeatabilities and effects of breed of boar, year, month, age of boar at collection and percentage of extender added were examined. Boar repeatabilities were 0.32 for post-thaw rating and 0.22 for loss in percentage motile sperm. Breed of boar was not significant. Year was significant, and percentage motile sperm recovered declined from 1976 through 1980. Semen collected between March and May had the highest post-thaw rating. As age of boar increased, post-thaw rating declined and loss in percentage motile sperm increased. As the percentage of extender added to semen increased, so did the loss in percentage motile sperm after freezing. Correlations between post-thaw rating and fresh semen motility score, volume, concentration and percentage motile sperm were 0.24, 0.03, −0.20 and 0.25, respectively. Respective correlations of fresh semen measures with loss in percentage motile sperm after freezing were 0.15, 0.01, 0.17 and 0.23. The current practice of standardizing fresh semen to a given concentration before freezing does not result in a standard frozen-thawed product with respect to percentage motile sperm. Key words: Boar, semen, freezing, thawing, motility

scholarly journals A new sperm selection criterion for cryopreservation of boar semen

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Monika Trzcińska ◽  
Magdalena Bryła
Keyword(s):  
Sperm Quality ◽  
Selection Criterion ◽  
Motile Sperm ◽  
Before And After ◽  
Viable Sperm ◽  
Sperm Membrane ◽  
Boar Semen ◽  
Acrosome Integrity ◽  
Fresh Semen

AbstractThis study aimed to define potential markers that could determine the suitability of ejaculate for cryopreservation. Fresh semen from eleven boars (4–7 ejaculates/boar), regardless of their sperm motility, was subjected to a cryopreservation procedure. The sperm quality before and after freezing was assessed based on the sperm membrane permeability and acrosome integrity. The results showed that it was possible to effectively cryopreserve ejaculates below the accepted standards of 70–80% of fresh motile sperm and still obtain a high cryosurvival rate. Moreover, a significant correlation was found between the percentage of viable sperm with apoptotic-like changes, viable sperm with reacted acrosomes, and the cryosurvival rate. The proposed markers for assessing the quality of fresh semen could be used to predict the success of cryopreservation procedures.

scholarly journals Cryopreservation and quality assessment of boar semen collected from bulk samples

2019 ◽  
Vol 64 (No. 5) ◽  
pp. 209-216 ◽  
Author(s):  
R Ratchamak ◽  
T Vongpralub ◽  
W Boonkum ◽  
V Chankitisakul
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Bulk Sample ◽  
Energy Status ◽  
Semen Volume ◽  
Boar Semen ◽  
Live Sperm ◽  
Fresh Semen

The purpose of this study was to examine sperm quality after cryopreservation of ejaculates collected as a bulk sample, which is routinely part of semen collection, and to compare this quality with the sperm-rich fraction in boars. Ejaculates were collected as sperm-rich fractions (SRF) and bulk samples (BE) using a gloved-hand technique. Fresh semen quality in terms of semen volume, sperm concentration, total sperm motility and pH were conventionally evaluated. Then, semen was cryopreserved using the liquid nitrogen vapour method. The post-thaw sperm quality was evaluated by assessing sperm motility, live sperm with normal apical ridge and high mitochondrial energy status, lipid peroxidation was evaluated using CASA and fluorescent multiple staining and MDA levels were determined using a spectrophotometer, respectively. In terms of fresh semen quality, sperm motility in fresh semen did not differ significantly between the two groups. The treatment with the greater mean volume (BE; P &lt; 0.05) had a lower mean sperm concentration (P &lt; 0.05); meanwhile, the mean ejaculate pH collected as BE was more basic compared with SRF (P &lt; 0.05). However, there were no significant post-thaw quality changes between sperm-rich fractions and bulk samples of semen. In conclusion, ejaculates can be collected as bulk samples without the need to classify fractions for boar semen cryopreservation.

scholarly journals Effects of Tris (hydroxymethyl) aminomethane on the quality of frozen-thawed boar spermatozoa

2019 ◽  
Vol 67 (1) ◽  
pp. 106-114
Author(s):  
Zhao Namula ◽  
Fuminori Tanihara ◽  
Manita Wittayarat ◽  
Maki Hirata ◽  
Nhien Thi Nguyen ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Control Group ◽  
Freezing And Thawing ◽  
Control Groups ◽  
Boar Semen ◽  
Boar Spermatozoa ◽  
Time Point

Tris (hydroxymethyl) aminomethane (Tris) has been used as a pH regulator for buffering the pH of dilution extenders for boar semen, such as the Modena extender. The purpose of the present study was to assess the effects of Tris supplementation at different concentrations (0, 8, 24 and 72 μM) into the freezing extender on the quality and fertilising capacity of frozen-thawed boar spermatozoa. The results showed that the supplementation of 24 μM of Tris gave significantly higher percentages of sperm viability and plasma membrane integrity than those of the control group at any time point of assessment (0 h and 3 h post-thawing) (P < 0.05). However, there were no significant differences in the acrosome integrity parameter among the groups. Higher percentages of sperm motility were observed in the spermatozoa cryopreserved with 24 μM of Tris compared to the control groups when the samples were analysed 0 h after thawing (P < 0.05). However, an increase of the Tris concentration to 72 μM did not enhance the sperm motility parameters. The total numbers of fertilised oocytes and blastocysts obtained with spermatozoa frozen with 24 μM Tris were significantly higher than those of the control group without Tris (P < 0.05). In conclusion, the supplementation of 24 μM Tris into the freezing extender contributes to a better boar sperm quality and fertilising capacity after the process of freezing and thawing.

scholarly journals Myoinositol Supplementation of Freezing Medium Improves the Quality-Related Parameters of Dog Sperm

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1038 ◽  
Author(s):  
Ahmad Yar Qamar ◽  
Xung Fang ◽  
Min Jung Kim ◽  
Jongki Cho
Keyword(s):  
Oxidative Stress ◽  
Semen Quality ◽  
Sperm Quality ◽  
Control Group ◽  
Oxygen Species ◽  
Sperm Viability ◽  
Freezing Medium ◽  
And Function ◽  
Fresh Semen ◽  
Lower Expression

Oxidative stress during freeze–thaw procedures results in reduced semen fertility. A decrease in free radical levels can improve the post-thaw sperm quality. We examined the effects of myoinositol supplementation in freezing medium on the structure and function of cryopreserved dog sperm. Pooled ejaculates were diluted with buffer without or with myoinositol (1 or 2 mg/mL). Analysis of fresh semen revealed that the optimal concentration of myoinositol was 1 mg/mL, and this concentration was used in further experiments. Post-thaw semen quality in the myoinositol-supplemented group was superior (p < 0.05) compared with that in the control group in terms of motility (57.9 ± 0.4% vs. 47.8 ± 0.2%), sperm viability (57.5 ± 0.5% vs. 44.6 ± 0.6%), intact plasma membrane (56.6 ± 0.4% vs. 46.2 ± 0.6%), and acrosome membrane (59.3 ± 0.5% vs. 51.8 ± 0.5%). In addition, sperm in the myoinositol-supplemented group showed a significantly lower expression of pro-apoptotic (BAX) and mitochondrial reactive oxygen species (ROS) modulator (ROMO1) genes but higher expression of anti-apoptotic (BCL2), and protamine-related (PRM2 and PRM3) genes compared with that in the control group. Therefore, myoinositol supplementation before freezing can protect against oxidative stress and improve post-thaw dog sperm quality.

scholarly journals Ameliorative effect of Allium atroviolaceum on sperm quality in cyclophosphamide-treated mice

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Alireza Hosseini ◽  
Mehrdad Shahrani ◽  
Shirin Asgharian ◽  
Maryam Anjomshoa ◽  
Ayoob Rostamzadeh ◽  
...  
Keyword(s):  
Side Effects ◽  
Alkylating Agent ◽  
Sperm Quality ◽  
Sperm Count ◽  
Reproductive Toxicity ◽  
Herbal Drugs ◽  
Sperm Viability ◽  
Complementary Treatment ◽  
Ameliorative Effect ◽  
Alternative Drugs

Abstract Background Cyclophosphamide (CP) is an anti-neoplastic alkylating agent that is extensively used in different chemotherapy regimens. Adverse effects on the reproductive system, especially spermatogenesis, are one of the most important side effects of this drug. It is medically essential to use complementary and alternative drugs. Herbal drugs have long been used as a complementary treatment. Our purpose was to study the effect of hydroalcoholic Allium atroviolaceum L. extract on spermatogenesis in CP-treated mice. Results CP affected a significant decrease in sperm count, motility, viability, and morphology. Sperm count was significantly higher in the all extract groups than in the group of control (p<0.001) and CP group (p<0.001, p<0.01). Sperm motility was significantly greater in the extract (100 and 200mg/kg) groups than in the group of control (p<0.05 and <0.001). Sperm immotility and rotational movement were significantly higher in the CP group than in the CP+extract groups (p<0.001). The sperm viability was significantly greater in the CP+extract (200mg/kg) group than in the CP group (p<0.001). The number of headless sperm, sperm with initial tail, with coiled tail, and sperm with curved body, was significantly lower in the CP+extract (200mg/kg) group than in the CP group (p<0.001). Conclusion A. atroviolaceum extract treatment significantly improved CP-induced reproductive toxicity.

Effect of lipid content of diet on cholesterol content and cholesterogenic enzymes of European eel liver

Lipids ◽  
1993 ◽  
Vol 28 (10) ◽  
pp. 913-916 ◽  
Author(s):  
C. Burgos ◽  
M. F. Zafra ◽  
M. Castillo ◽  
E. Garcia-Peregrin
Keyword(s):  
Lipid Content ◽  
Cholesterol Content ◽  
European Eel
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