Hydrophilic coatings for biomedical applications in and ex vivo

2012 ◽  
pp. 3-42 ◽  
Author(s):  
P. Wyman
Keyword(s):  
Biomedical Applications ◽  
Ex Vivo ◽  
Hydrophilic Coatings

scholarly journals Biomedical Uses of Sulfobetaine-Based Zwitterionic Materials

2020 ◽  
Vol 02 (04) ◽  
pp. 342-357
Author(s):  
Francesco Zaccarian ◽  
Matthew B. Baker ◽  
Matthew J. Webber
Keyword(s):  
Biomedical Applications ◽  
Foreign Body Reaction ◽  
Inflammatory Responses ◽  
Cellular Adhesion ◽  
Review Of The Literature ◽  
Future Prospects ◽  
Protein Fouling ◽  
Biomedical Device ◽  
The Many ◽  
Hydrophilic Coatings

Protein fouling can render a biomedical device dysfunctional, and also serves to nucleate the foreign body reaction to an implanted material. Hydrophilic coatings have emerged as a commonly applied route to combat interface-mediated complications and promote device longevity and limited inflammatory response. While polyethylene glycol has received a majority of the attention in this regard, coatings based on zwitterionic moieties have been more recently explored. Sulfobetaines in particular constitute one such class of zwitterions explored for use in mitigating surface fouling, and have been shown to reduce protein adsorption, limit cellular adhesion, and promote increased functional lifetimes and limited inflammatory responses when applied to implanted materials and devices. Here, we present a focused review of the literature surrounding sulfobetaine, beginning with an understanding of its chemistry and the methods by which it is applied to the surface of a biomedical device in molecular and polymeric forms, and then advancing to the many early demonstrations of function in a variety of biomedical applications. Finally, we provide some insights into the benefits and challenges presented by its use, as well as some outlook on the future prospects for using this material to improve biomedical device practice by addressing interface-mediated complications.

scholarly journals A Tumbling Magnetic Microrobot System for Biomedical Applications

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 861
Author(s):  
Elizabeth E. Niedert ◽  
Chenghao Bi ◽  
Georges Adam ◽  
Elly Lambert ◽  
Luis Solorio ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Biomedical Applications ◽  
Imaging System ◽  
Ex Vivo ◽  
Negative Control ◽  
Circular Path ◽  
Rotational Axis ◽  
Significant Difference

A microrobot system comprising an untethered tumbling magnetic microrobot, a two-degree-of-freedom rotating permanent magnet, and an ultrasound imaging system has been developed for in vitro and in vivo biomedical applications. The microrobot tumbles end-over-end in a net forward motion due to applied magnetic torque from the rotating magnet. By turning the rotational axis of the magnet, two-dimensional directional control is possible and the microrobot was steered along various trajectories, including a circular path and P-shaped path. The microrobot is capable of moving over the unstructured terrain within a murine colon in in vitro, in situ, and in vivo conditions, as well as a porcine colon in ex vivo conditions. High-frequency ultrasound imaging allows for real-time determination of the microrobot’s position while it is optically occluded by animal tissue. When coated with a fluorescein payload, the microrobot was shown to release the majority of the payload over a 1-h time period in phosphate-buffered saline. Cytotoxicity tests demonstrated that the microrobot’s constituent materials, SU-8 and polydimethylsiloxane (PDMS), did not show a statistically significant difference in toxicity to murine fibroblasts from the negative control, even when the materials were doped with magnetic neodymium microparticles. The microrobot system’s capabilities make it promising for targeted drug delivery and other in vivo biomedical applications.

Flexible Pressure Sensors for Biomedical Applications: From Ex Vivo to In Vivo

2020 ◽  
Vol 7 (17) ◽  
pp. 2000743 ◽  
Author(s):  
Lin Li ◽  
Jiahong Zheng ◽  
Jing Chen ◽  
Zebang Luo ◽  
Yi Su ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Ex Vivo ◽  
Pressure Sensors ◽  
Flexible Pressure Sensors

scholarly journals Novel thermoresponsive polymer outperforming Pluronic® F127

2020 ◽  
Author(s):  
Anna Constantinou ◽  
Valeria Nele ◽  
James Doutch ◽  
Roman Moiseev ◽  
Vitaliy Khutoryanskiy ◽  
...  
Keyword(s):  
Self Assembly ◽  
Biomedical Applications ◽  
Ex Vivo ◽  
Structural Characteristics ◽  
Thermoresponsive Polymer ◽  
Scanning Calorimetry ◽  
Thermoresponsive Polymers ◽  
Crosslinked Networks ◽  
Gelation Concentration ◽  
Physically Crosslinked

Abstract Thermoresponsive polymers featuring the appropriate combination of structural characteristics, i.e. architecture, composition, and molar mass (MM), can form physically crosslinked networks in a solvent upon changes in temperature. This fascinating class of polymers finds utility in various sectors such as formulation science and tissue engineering. Here, we report a novel thermoresponsive triblock terpolymer which out-performs the most commonly used and commercially available thermoresponsive polymer, Poloxamer P407 (also known as Pluronic® F127) in terms of gelation concentration. Specifically, the in-house synthesised polymer forms gels at lower concentrations that is an advantage in biomedical applications. To elucidate the differences in their macroscale gelling behaviour, we investigate their micellization via differential scanning calorimetry, and their nanoscale self-assembly behaviour in detail by means of small-angle neutron scattering by simultaneously recording their rheological properties (Rheo-SANS). Two different gelation mechanisms for the two polymers are revealed and proposed. Ex vivo gelation study upon intracameral injections demonstrated excellent potential for its application to improve drug residence in the eye.

Photophysics and ex vivo biodistribution of β-cyclodextrin-meso-tetra(m-hydroxyphenyl)porphyrin conjugate for biomedical applications

2014 ◽  
Vol 13 (8) ◽  
pp. 1185-1191 ◽  
Author(s):  
V. Kirejev ◽  
A. R. Gonçalves ◽  
C. Aggelidou ◽  
I. Manet ◽  
J. Mårtensson ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Ex Vivo ◽  
Ex Vivo Biodistribution

Photophysics andex vivobiodistribution of porphyrin (meso-tetra(m-hydroxyphenyl)porphyrin;mTHPP) and porphyrin-cyclodextrin conjugate (β-cyclodextrin-meso-tetra(m-hydroxyphenyl)porphyrin; CD-mTHPP) were investigated and compared.

scholarly journals In Vitro and Ex Vivo Investigation of the Effects of Polydopamine Nanoparticle Size on Its Antioxidant and Photothermal Properties: Implications for Biomedical Applications

2022 ◽  
Author(s):  
Alessio Carmignani ◽  
Matteo Battaglini ◽  
Edoardo Sinibaldi ◽  
Attilio Marino ◽  
Veronica Vighetto ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Ex Vivo ◽  
Nanoparticle Size

scholarly journals Biomedical applications of copper-free click chemistry: in vitro, in vivo, and ex vivo

2019 ◽  
Vol 10 (34) ◽  
pp. 7835-7851 ◽  
Author(s):  
Eunha Kim ◽  
Heebeom Koo
Keyword(s):  
Click Chemistry ◽  
Chemical Reactions ◽  
Biomedical Applications ◽  
Ex Vivo ◽  
The Body ◽  
Cell Surfaces ◽  
Cell Cytosol ◽  
Biomedical Fields

Copper-free click chemistry has resulted in a change of paradigm, showing that artificial chemical reactions can occur on cell surfaces, in cell cytosol, or within the body. It has emerged as a valuable tool in biomedical fields.

scholarly journals Hydrogen Peroxide Sensors for Biomedical Applications

Chemosensors ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 64 ◽  
Author(s):  
Jakob Meier ◽  
Eric M Hofferber ◽  
Joseph A Stapleton ◽  
Nicole M Iverson
Keyword(s):  
Hydrogen Peroxide ◽  
Human Body ◽  
Biomedical Applications ◽  
Ex Vivo ◽  
Biological Systems ◽  
Reactive Species

Hydrogen peroxide (H2O2) is an important molecule within the human body, but many of its roles in physiology and pathophysiology are not well understood. To better understand the importance of H2O2 in biological systems, it is essential that researchers are able to quantify this reactive species in various settings, including in vitro, ex vivo and in vivo systems. This review covers a broad range of H2O2 sensors that have been used in biological systems, highlighting advancements that have taken place since 2015.

scholarly journals A Tumbling Magnetic Microrobot System for Biomedical Applications

2020 ◽  
Author(s):  
Elizabeth E. Niedert ◽  
Chenghao Bi ◽  
Georges Adam ◽  
Elly Lambert ◽  
Luis Solorio ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Biomedical Applications ◽  
Imaging System ◽  
Ex Vivo ◽  
Negative Control ◽  
Circular Path ◽  
Rotational Axis ◽  
Significant Difference

AbstractA microrobot system comprised of an untethered tumbling magnetic microrobot, a two degree of freedom rotating permanent magnet, and an ultrasound imaging system has been developed for in vitro and in vivo biomedical applications. The microrobot tumbles end-over-end in a net forward motion due to applied magnetic torque from the rotating magnet. By turning the rotational axis of the magnet, two-dimensional directional control is possible and the microrobot was steered along various trajectories, including a circular path and P-shaped path. The microrobot is capable of moving over the unstructured terrain within a murine colon in in vitro, in situ, and in vivo conditions, as well as a porcine colon in ex vivo conditions. High frequency ultrasound imaging allows for real-time determination of the microrobot’s position while it is optically occluded by animal tissue. When coated with a fluorescein payload, the microrobot was shown to release the majority of the payload over a one hour time period in phosphate-buffered saline. Cytotoxicity tests demonstrated that the microrobot’s constituent materials, SU-8 and polydimethylsiloxane (PDMS), did not show a statistically significant difference in toxicity to murine fibroblasts from the negative control, even when the materials were doped with magnetic neodymium microparticles. The microrobot system’s capabilities make it promising for targeted drug delivery and other in vivo biomedical applications.

scholarly journals A practical guide for investigating cardiac physiology using living myocardial slices

2020 ◽  
Vol 115 (6) ◽  
Author(s):  
S. A. Watson ◽  
A. Dendorfer ◽  
T. Thum ◽  
F. Perbellini
Keyword(s):  
Biomedical Applications ◽  
Ex Vivo ◽  
Research Field ◽  
Research Model ◽  
Cardiac Physiology ◽  
Practical Guide ◽  
Data Collection And Analysis ◽  
Reasonable Cost ◽  
Technological Developments ◽  
Quality And Reliability

Abstract Ex vivo multicellular preparations are essential tools to study tissue physiology. Among them, the recent methodological and technological developments in living myocardial slices (LMS) are attracting increasing interest by the cardiac research field. Despite this, this research model remains poorly perceived and utilized by most research laboratories. Here, we provide a practical guide on how to use LMS to interrogate multiple aspects of cardiac function, structure and biochemistry. We discuss issues that should be considered to conduct successful experiments, including experimental design, sample preparation, data collection and analysis. We describe how laboratory setups can be adapted to accommodate and interrogate this multicellular research model. These adaptations can often be achieved at a reasonable cost with off-the-shelf components and operated reliably using well-established protocols and freely available software, which is essential to broaden the utilization of this method. We will also highlight how current measurements can be improved to further enhance data quality and reliability to ensure inter-laboratory reproducibility. Finally, we summarize the most promising biomedical applications and envision how living myocardial slices can lead to further breakthroughs.

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