HPLC Analysis of Amino Acid and Antioxidant Composition of Three Medicinal Plants of (Pithoragarh) Uttarakhand Himalayas

2017 ◽  
Vol 6 (4) ◽  
Author(s):  
Kundan Prasad
Keyword(s):  
Amino Acid ◽  
Medicinal Plants ◽  
Hplc Analysis

scholarly journals Determination of amino acids in human biological fluids by high-performance liquid chromatography: critical review

Amino Acids ◽  
2021 ◽  
Author(s):  
Grażyna Gałęzowska ◽  
Joanna Ratajczyk ◽  
Lidia Wolska
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
High Performance ◽  
Hplc Analysis ◽  
Limit Of Detection ◽  
Biological Fluids ◽  
Analytical Techniques ◽  
Epidemiological Studies ◽  
Preparation Conditions

AbstractThe quantitation and qualification of amino acids are most commonly used in clinical and epidemiological studies, and provide an excellent way of monitoring compounds in human fluids which have not been monitored previously, to prevent some diseases. Because of this, it is not surprising that scientific interest in evaluating these compounds has resurfaced in recent years and has precipitated the development of a multitude of new analytical techniques. This review considers recent developments in HPLC analytics on the basis of publications from the last few years. It helps to update and systematize knowledge in this area. Particular attention is paid to the progress of analytical methods, pointing out the advantages and drawbacks of the various techniques used for the preparation, separation and determination of amino acids. Depending on the type of sample, the preparation conditions for HPLC analysis change. For this reason, the review has focused on three types of samples, namely urine, blood and cerebrospinal fluid. Despite time-consuming sample preparation before HPLC analysis, an additional derivatization technique should be used, depending on the detection technique used. There are proposals for columns that are specially modified for amino acid separation without derivatization, but the limit of detection of the substance is less beneficial. In view of the fact that amino acid analyses have been performed for years and new solutions may generate increased costs, it may turn out that older proposals are much more advantageous.

Amino acid disorders detected by quantitative amino acid HPLC analysis in Thailand: An eight-year experience

2012 ◽  
Vol 413 (13-14) ◽  
pp. 1141-1144 ◽  
Author(s):  
Nithiwat Vatanavicharn ◽  
Pisanu Ratanarak ◽  
Somporn Liammongkolkul ◽  
Achara Sathienkijkanchai ◽  
Pornswan Wasant
Keyword(s):  
Amino Acid ◽  
Hplc Analysis

FIBRINOGEN BIRMINGHAM; A NEW CONGENITAL HETERODIMERIC DYSFIBRINOGENEMIA WITH DEFECTIVE FIBRINOPEPTIDE A RELEASE (Aα 16 Arg→His)

1987 ◽  
Author(s):  
K R Siebenlist ◽  
J T Prchal ◽  
M W Masesson
Keyword(s):  
Amino Acid ◽  
Amino Acid Analysis ◽  
Hplc Analysis ◽  
Biochemical Characterization ◽  
Clinical Manifestations ◽  
Severe Bleeding ◽  
Fibrinopeptide A ◽  
History Of ◽  
Bleeding Episodes

Aα 16 Arg→His substitutions are common forms of congenital dysfibrinogenemias. Clinical manifestations range from asymptomatic to moderate hemorrhagic tendencies. Biochemical characterization of one such heterozygotic individual (Fibrinogen Louisville, Galanakis, etal. Ann NY Acad Sci 408:644,1983) indicated that only homodimeric fibrinogen molecules (i.e., containing either normal or abnormal Aα chains) were present. We isolated fibrinogen from the plasma of a 23 year old patient with a history of easy bruising and several recent moderate to severe bleeding episodes. Coagulability with reptilase was 677 (65-70%; n=5) whereas with thrombin (Ha) it approached 100%, depending directly upon the time of incubation with enzyme. HPLC analysis of Ila-induced fibrinopeptide release demonstrated the presence of an abnormal A-peptide (A*), amounting to 50% of the total, which was released more slowly than the normal A-peptide (A). Amino acid analysis of A* demonstrated the absence of Arg and the presence of His. Carboxypeptidase digestion confirmed the structure of A* as Aα 16 Arg-→ His. The clot and the soluble clot liquor resulting from reptilase treatment were separated and each was then further treated with Ilato release A*. HPLC analysis indicated that 31% of the total A* present in the sample was associated with the reptilase clot and 697 remained in the clot liquor. This distribution of A* suggests that Fibrinogen Birmingham, unlike Fibrinogen Louisville, contains heterodimeric molecules that are incorporated into the reptilase clottable fraction. This finding is consistent with a process of random hepatic assembly of dimeric fibrinogen molecules in a heterozygotic individual.

scholarly journals Absolute Stereochemistry of the β-Hydroxy Acid Unit in Hantupeptins and Trungapeptins

2016 ◽  
Vol 11 (1) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Deepak Kumar Gupta ◽  
Gary Chi Ying Ding ◽  
Yong Chua Teo ◽  
Lik Tong Tan
Keyword(s):  
Amino Acid ◽  
Structural Feature ◽  
Absolute Configuration ◽  
Hydroxy Acid ◽  
Hplc Analysis ◽  
The Absolute ◽  
Absolute Stereochemistry ◽  
Common Structural Feature ◽  
Acid Unit

The β-hydroxy/amino acid unit is a common structural feature of many bioactive marine cyanobacterial depsipeptides. In this study, the absolute stereochemistry of the β-hydroxy acid moieties in hantupeptins and trungapeptins were determined through their synthesis and HPLC analysis of the Mosher ester derivatives. Synthesis of two3-hydroxy-2-methyloctanoic acid (Hmoa) stereoisomers, (2 S,3 R)-Hmoa and (2 S,3 S)-Hmoa, were achieved using diastereoselective asymmetric method and the retention times of all four Hmoa isomers were established indirectly by RPLC-MS analysis of their Mosher ester derivative standards. Based on the retention times of the standards, the absolute configuration of the Hmoa unit in hantupeptin C (3) and trungapeptin C (6) was assigned as (2 R,3 S)- and (2 S,3 R)-Hmoa, respectively. The use of the Mosher's reagents, coupled with HPLC analysis, provided a viable alternative to the absolute stereochemical determination of β-hydroxy acid units in depsipeptides.

scholarly journals Analisis Profil Protein dan Asam Amino Sarang Burung Walet (Collocalia fuchiphaga)Asal Painan

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Lina Elfita
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Aspartic Acid ◽  
High Performance ◽  
Hplc Analysis ◽  
Protein Profile ◽  
Essential Amino Acids ◽  
Bird Nest ◽  
Sds Page ◽  
West Sumatra

Abstrak Penelitian tentang profil protein sarang burung wallet masih terbatas, terutama sarang burung walet dari Indonesia. Oleh karena itu, penelitian ini bertujuan untuk menganalisa profil protein dan asam amino sarang burung walet yang berasal dari daerah Painan, Kabupaten Pesisir Selatan, Sumatera Barat. Analisis protein dilakukan dengan menggunakan SDS-PAGE, sedangkan analisis asam amino dilakukan dengan menggunakan kromatografi cair kinerja tinggi (KCKT). Analisa ekstrak air sarang burung walet dengan SDS-PAGE menunjukan bahwa sarang burung walet terdiri dari 6 protein. Keenam protein tersebut mempunyai bobot molekul masing-masing 147.2 kDa, 142.6 kDa, 133.4 kDa, 73.3 kDa, 66.2 kDa dan 37.7 kDa. Dari analisa asam amino burung walet dengan KCKT didapatkan 16 asam amino yang terkandung dalam sarang burung wallet, yang terdiri dari 7 jenis asam amino esensial yaitu Histidin (2.31%), Leusin (3.84%), Treonin (3.82%), Valin (3.93%), Metionin (0.48%), Isoleusin (1.80%), Fenilalanine (4.49%)  dan 9 asam amino non esensial yaitu Asam Serin (4.56%), Aspartat (4.48%), Arginin (3.93%), Lisin (2.34 %), Prolin (3.64%),  Asam glutamate (3.65%), Glisin (1.87%), Alanin (1.31%), Tirosin (3.92%). Serin merupakan asam amino dengan kadar tertinggi (4.56%), diikuti dengan Fenil alanine (4.49%) dan Asam aspartate (4.48%). Kandungan asam amino ini sedikit berbeda dengan kandungan asam amino sarang burung walet dari daerah dan negara lain. Kata kunci: sarang burung walet, protein, asam amino Abstract Study on protein profile of bird nest is still limited particularly protein profile of bird nest from Indonesia has not been reported. Therefore, this study was aimed to analyze protein profile and amino acid composition of bird nest from Painan, Pesisir Selatan Distric, West Sumatra. Protein analysis was performed by SDS-PAGE, and high performance liquid chromatography (HPLC) was used for amino acid analysis. SDS-PAGE analysis showed  six bands, which molecular weigh of 147.2 kDa, 142.6 kDa, 133.4 kDa, 73.3 kDa, 66.2 kDa and 37.7 kDa, respectively. On the other hand, HPLC analysis demonstrated that bird nest was composed of 16 amino acids. Seven of them were essential amino acids; histidine (2.31%), leucine (3.84%), threonine (3.82%),  valine (3.93%), methionine (0.48%), isoleucine (1.80%), phenylalanine (4.49%), and nine of them were non-essential amino acids; serine (4.56%), aspartic acid (4.48%), arginine (3.93%), lysine (2.34%), proline (3.64%), glutamic acid (3.65%), glycine (1.87%), alanine (1.31%), tyrosine (3.92%). Serine was the highest percentage of amino acid in the bird nest (4.56%), followed by phenylalanine (4.49%) and aspartic acid (4.48%). Composition of amino acid in this bird nest was slightly different with composition of amino acid in bird nest from other area. Keywords : bird nest, protein profile, amino acids

On-line HPLC analysis of PTH-amino acids derived from Edman degradation of proteins and peptides: Optical sensor controlled sample injection

1989 ◽  
Vol 54 (4) ◽  
pp. 940-944 ◽  
Author(s):  
Manfred Pavlík ◽  
Jiří Jehnička ◽  
Vladimír Kostka
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Hplc Analysis ◽  
Edman Degradation ◽  
Isocratic Elution ◽  
Sample Injection ◽  
Optoelectronic Sensor ◽  
On Line ◽  
The Moment ◽  
Proteins And Peptides

A new principle in the system of identification and quantitation of amino acid phenylthiohydantoins derived from automated Edman degradation of proteins and peptides is described. The beginning of the on-line HPLC analysis of PTH's, the actuation of the valve and the sample injection are controlled by an optoelectronic sensor placed at the sample loop outlet. At the moment the loop has been loaded the sensor actuates the injection valve thus starting the chromatography run. This arrangement eliminates the possibility of incomplete (irreproducible) loading of the loop or of void injection. The chromatographic separation of the PTH's is carried out by isocratic elution.

scholarly journals HPLC Analysis of Chemical Composition of Selected Jordanian Medicinal Plants and Their Bioactive Properties

2018 ◽  
Vol 34 (5) ◽  
pp. 2397-2403
Author(s):  
Fuad Al-Rimawi ◽  
Fadi Alakhras ◽  
Wael A. Al-Zereini ◽  
Hammad K. Aldal'in ◽  
Saleh Abu-Lafi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Hplc Analysis ◽  
Room Temperature ◽  
Bacterial Strains ◽  
E Coli ◽  
Bioactive Properties

Three medicinal plants grown wild in Jordan, namely Achillea santolina L, Achillea fragrantisimma, Asteriscus graveolens (Forssk) Less, were extracted with ethyl acetate by continuous shaking at room temperature for three days. The antibacterial activity of the crude extract was evaluated. The extracts were analyzed for their phenolic and flavonoids content by HPLC-PDA. The HPLC analysis of the plant extracts revealed the presence of flavonoids and phenolic compounds in the three plant extracts. Results revealed a strong antibacterial activity of A. graveolens against three bacterial strains (B. subtilis, E. coli, and S.aureus) while A. fragrantissima inhibited the growth of B. subtilis. Bioactivities were attributed mainly to the immense content of phenol-based compounds in plants.

scholarly journals Biological Activities and Chemical Characterization ofCordia verbenaceaDC. as Tool to Validate the Ethnobiological Usage

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Edinardo Fagner Ferreira Matias ◽  
Erivânia Ferreira Alves ◽  
Beatriz Sousa Santos ◽  
Celestina Elba Sobral de Souza ◽  
João Victor de Alencar Ferreira ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Synergistic Effect ◽  
Crude Extract ◽  
Hplc Analysis ◽  
Antibacterial Effect ◽  
Biological Activities ◽  
Chemical Characterization ◽  
Analgesic Agents

Knowledge of medicinal plants is often the only therapeutic resource of many communities and ethnic groups. “Erva-baleeira”,Cordia verbenaceaDC., is one of the species of plants currently exploited for the purpose of producing a phytotherapeutic product extracted from its leaves. In Brazil, its major distribution is in the region of the Atlantic Forest and similar vegetation. The crude extract is utilized in popular cultures in the form of hydroalcoholic, decoctions and infusions, mainly as antimicrobial, antiinflammatory and analgesic agents. The aim of the present study was to establish a chemical and comparative profile of the experimental antibacterial activity and resistance modifying activity with ethnopharmacological reports. Phytochemical prospecting and HPLC analysis of the extract and fractions were in agreement with the literature with regard to the presence of secondary metabolites (tannins and flavonoids). The extract and fraction tested did not show clinically relevant antibacterial activity, but a synergistic effect was observed when combined with antibiotic, potentiating the antibacterial effect of aminoglycosides. We conclude that tests of antibacterial activity and modulating the resistance presented in this work results confirm the ethnobotanical and ethnopharmacological information, serving as a parameter in the search for new alternatives for the treatment of diseases.

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