scholarly journals Antioxidant and phytochemical analysis of volatile oil and extracts of Pinus wallichiana

2019 ◽  
Vol 4 (2) ◽  
pp. 37-40
Author(s):  
Pooja Sharma ◽  
Sahil Gupta ◽  
Nazli Bhatt ◽  
Shajaat Hussain Ahanger ◽  
Divya Gupta ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aqueous Extract ◽  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Chelating Activity ◽  
Pinus Wallichiana

The present study aims to explore the chemical constituents and antioxidant potential of Pinus wallichiana essential oil and extracts. Chemical composition of the essential oil was analyzed by GC-MS and result showed that the oil contain three major monoterpene compounds viz. α-pinene (48.6%), β-pinene (45.6%) and limonene (5.6%). Antioxidant potential of Pinus wallichiana was analyzed by three basic methods; DPPH radical scavenging method, FRAP assay and Fe2+ ion chelating activity. Among leaf and bark extracts, best radical scavenging, activity was determined by aqueous extract (IC50 values 20.83±0.8µg/ml) of bark followed by its methanol extract (IC50 value 25.9±1.6µg/ml). The aqueous extract of bark also exhibited better reducing and chelating activity than leaf extracts. Essential oil showed moderate radical scavenging and chelating activity but negligible reducing activity. Phytochemical analysis of extracts determined that the aqueous and methanol extracts of bark contain rich amount of poly phenol and flavonoids, in comparison to the leaf extracts. A significant correlation between the antioxidant activity and these phytoconstituents of the extracts has been observed. The results of the present study concluded that the bark of Pinus wallichiana is a potential source of active antioxidant constituents that could be further explored and exploited for numerous commercial applications.

scholarly journals Phytochemical Screening and in vitro Antioxidant Properties of Methanol and Aqueous Leaf Extracts of Geophila obvallata

2018 ◽  
pp. 1-11
Author(s):  
Iserhienrhien Lucky Osafanme ◽  
Okolie Paulinus Ngozi
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Scientific Basis ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Reducing Ability

Aim: This study investigated the phytochemical constituents and in vitro antioxidant properties of methanol and aqueous leaf extracts of Geophila obvallata using standard methods. Materials and Methods: The in vitro antioxidant assays carried out were 1, 1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging ability, Nitric oxide (NO•) radical scavenging activity assay, 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS•+) radical cation scavenging assay, ferric reducing properties and hydroxyl radical scavenging assays. Results: Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolic compounds, steroids, saponins, terpernoids and cardiac glycosides in both extracts. Relative to the aqueous extract, the methanol extract contained a higher amount of the secondary metabolites. However, both extracts exhibited appreciable and dose-dependent capacities for quenching DPPH, ABTS•+ and NO• free radicals, and potent ferric reducing ability to levels comparable to those of ascorbic acid. The crude methanol extract showed significantly increased (P<0.05) antioxidant activity than the aqueous extract. Conclusion: It was concluded that the extract possesses strong antioxidant properties due to its content of phytochemicals, and provides scientific basis for its ethno medicinal applications.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF LEAF EXTRACTS OF GREWIA HETEROTRICHA MAST

2016 ◽  
Vol 8 (4) ◽  
pp. 68 ◽  
Author(s):  
B. Usha ◽  
K. C. Pushpalatha
Keyword(s):  
Aqueous Extract ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Leaf Extracts

Objective: To investigate the presence of phytochemical components and to evaluate the in vitro antioxidant activity of pet. ether, chloroform, methanol and aqueous extracts of Grewia heterotricha mast leaves.Methods: The leaves of Grewia heterotricha mast were dried and extracts were prepared using a pet. ether, chloroform, methanol by soxhlet extraction method. The aqueous extract was prepared using distilled water by cold extraction method. The preliminary phytochemical analysis was carried out on aqueous, methanol, chloroform and pet. either leaf extracts of the plant using standard qualitative procedures. The total phenolic content (TPC) was estimated using modified Folin-Ciocalteau method, tannin content by Folin-Denis method and total flavonoids by aluminum chloride method. In vitro, antioxidant activities were evaluated by 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, H2O2 scavenging activity and FRAP assay.Results: The preliminary phytochemical analysis revealed the presence of complex bioactive constituents like phenols, tannins, alkaloids, terpenoids, flavonoids, saponins, steroids, glycosides, coumarins, proteins and carbohydrates. Methanolic extract showed highest total phenolic content (87.58±2.52 mg CE/g) than aqueous extract (78.46±5.36 mg CE/g). Higher tannin content was found in the aqueous extract (148.0±8.96 mg TAE/g). Total flavonoids were highest in chloroform extract (314.9±25.06 mg QE/g) followed by aqueous (242.98±32.42 mg QE/g) and methanolic extract (217.0±18.32 mg QE/g) and lowest in a pet. ether extract (188.86±23.35 mg QE/g). The methanolic extract had shown very significant DPPH radical scavenging activity (IC50 98.95 µg/ml) and H2O2 scavenging activity (IC50110.1µg/ml) compared to the standard ascorbic acid. Higher reducing ability was observed in methanol extract (131.8±11.67 mg AE/g).Conclusion: The results obtained reveal that the leaves of Grewia heterotricha mast have potent antioxidant property. The observed activity may be associated with bioactive components like phenolics, flavonoids present in the leaf extracts and could have greater importance as therapeutic agents in oxidative stress-related degenerative diseases. Further studies are needed in order to purify bioactive compounds responsible for the antioxidant property.

In vitro antioxidant activity and phytochemical analyses of Acalypha godseffiana (Euphobiaceae) leaf extracts

2021 ◽  
Vol 25 (1) ◽  
pp. 75-79
Author(s):  
S.O. Olubodun ◽  
G.E. Eriyamremu ◽  
M.E. Ayevbuomwan ◽  
C.I. Nzoputa
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anti Oxidant ◽  
In Vitro Antioxidant Activity

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

scholarly journals ANTIOXIDANT ACTIVITY OF LEAF EXTRACTS OF HEMIDESMUS INDICUS (L.) R.BR. (ASCLEPIADACEAE).

2017 ◽  
Vol 10 (6) ◽  
pp. 200
Author(s):  
Abdul Kaffoor H ◽  
Muthuraj K ◽  
Arumugasamy K
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Stable Free Radical ◽  
Hemidesmus Indicus

Objective: A number of Indian medicinal plants have been used for thousands of years in a traditional system of medicine. Hemidesmus indicus is an important member of the Asclepiadaceae family. It is an endemic to the southern Western Ghats, India. The aim of the study was to investigate the free radical scavenging activity of H. indicus. Methods: The aqueous and methanol leaf extracts of H. indicus were assayed for radical scavenging activity, using the stable free radical 2,2-diphenyl- 1-picryl-hydrazyl-hydrate and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid). Results: The results revealed that the IC50 values of aqueous extract of H. indicus were found to be higher than that of the other solvent extracts. The free radical scavenging activity of the plant extracts may be due to the presence of phytoconstituents. Conclusion: In all the methods, the aqueous extract has exhibited the good scavenging activity and this arises that the plant has a potential antioxidant agent.

scholarly journals In-vitro Antioxidant Activity and Preliminary Phytochemical Analysis of Different Leaf Extracts of Hemionitis arifolia

2021 ◽  
pp. 281-291
Author(s):  
Rajendran Raja Priya ◽  
N. Bhadusha ◽  
Veramuthu Manivannan ◽  
Thanthoni Gunasekaran
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Hydroalcoholic Extract ◽  
Reducing Power Assay

Objective: To evaluate the preliminary phytochemical content and antioxidant potential of the hydroalcoholic leaf extracts of Hemionitis arifolia. Methods: Total phenolic, flavonoid and alkaloid contents were evaluated using spectrophotometric methods. The free radical scavenging activity of the leaf hydroalcoholic extract were evaluated against DPPH+, ABTS+, Reducing power assay and nitric oxide assay were determined. Results: The hydroalcoholic concentrate of H. arifolia uncovered the most elevated polyphenol content when contrasted and the other phytoconstituents. Absolute phenol content of the hydroalcoholic separate was observed to be 31.78%, flavonoid content is 1.02% and Alkaloid content is 30.40% individually. The Solvent concentrates showed huge cell reinforcement movement, with hydroalcoholic extract. ABTS Assay, DPPH assay, Reducing power assay and Nitric oxide assay where the Inhibition concentration were 667.75µg/ml, 734.25 µg/ml, 791.58 µg/ml and 899.67 µg/ml. Conclusion: This study suggests that hydroalcoholic leaf extracts of H. arifolia could be a potential source of natural antioxidant and justifies its use in ethno-medicine.

scholarly journals A Comparative Study between Plant and Callus Extracts of Abutilon indicum (L.) Sweet: Antioxidant, Antibacterial, Antidiabetic and Anti-Proliferative Activity

2020 ◽  
pp. 13-24
Author(s):  
A. Ayisha Sireen ◽  
J. Anbumalarmathi
Keyword(s):  
Ethyl Acetate ◽  
Aqueous Extract ◽  
Proliferative Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Ic50 Value ◽  
Ethyl Acetate Extracts ◽  
Abutilon Indicum

Abutilon indicum is consider to be used in the traditional system of medicine. It is found in tropical and subtropical regions of the world. It is used to treat various diseases. This plant does not cause any side effects to humans. As the plant has wide variety of medicinal properties, the present study aimed to comparative between plant and callus extract of Abutilon indicum (L.) sweet for antioxidant, antibacterial, antidiabetic and anti- proliferative activity. The highest percentage of callus induction (89.50%) and callus weight (1.26 g) was observed in T5 (MS + 2, 4-D (2.5 mg/l) + BAP (2 mg/l) and T8 [IBA (4 mg/l)] respectively. Phytochemical analysis of aqueous and ethyl acetate extracts of A. indicum in vivo plant and in vitro grown callus showed the presence of alkaloids, flavonoids, phenols, carbohydrates, glycosides, protein, terpenoids, saponins, tannins and coumarin. The total phenolic content was high in aqueous extract of callus (30.68 mg TAE/g). Maximum DPPH radical scavenging activity was found in aqueous extract of callus (86%) with IC50 value of 68.49 µg/ml. FT-IR analysis of aqueous extract of A. indicum plant and callus showed the presence of characteristic stretching at 2930.28 and 2927.75 indicating the presence of C-H stretching respectively. GC-MS analysis revealed the presence of 17 compounds in ethyl acetate plant extract, whereas 7 compounds in ethyl acetate callus extract such as tetradecane, 1-chloro, Sulfurous acid 2-prophytridecyl ester and 1- ethyl-3-[2-(octadecylthio) ethyl] thiourea. The ethyl acetate extracts of callus and plant and was found to be effective against Bacillus subtilis (3.1 mm) and Staphylococcus aureus (2.9 mm). Maximum α-amylase inhibitory activity was observed in aqueous callus extract (32.65%) with IC50 value of 833.61 µg/ml. HeLa cell viability was found to be 26.8% and 21.8% in plant and callus extract respectively.

scholarly journals Evaluation of Phyto Chemical Biochemical and In Vitro Antioxidant Potential of Angelica Glauca Grown at High Altitude Areas of Western Himalayas

2021 ◽  
Vol 6 (2) ◽  
pp. 117-121
Author(s):  
Swati Arya ◽  
H.K. Pandey ◽  
A. Singh ◽  
H.S. Meena ◽  
M. Bala
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Biochemical Parameter ◽  
Antioxidant Potential ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Western Himalayas ◽  
Hydroalcoholic Extract ◽  
Angelica Glauca

Angelica glauca Edgew is an important medicinal and aromatic herb (family Apiaceae). The roots of A. glauca commonly used as spices by local peoples. In the present study, the phytochemical constituents, biochemical parameters and, in-vitro antioxidant activity of A. glauca roots collected from the Himalayan region have been studied. For preliminary phytochemical analysis, the hydroalcoholic and aqueous root extract of Angelica glauca were screened for the presence of carbohydrates, protein, alkaloids, glycosides, sterols, triterpenes, saponin, tannins, phenols, flavonoids, and coumarin. The biochemical parameter and in-vitro antioxidant potential of Angelica glauca were analysed by using standard methods. Phytochemical analysis of extract showed major classes of phytochemicals constituents such as carbohydrates, protein, alkaloids, glycosides, sterols, triterpenes, saponin, tannins, phenols, flavonoids, and coumarin. The results from the current study demonstrated that A.glauca roots contained carbohydrate (21±0.72 %), crude protein (12.7±0.31 %), total ash (3.86±0.034 %), dietary fiber (18.9±0.14 %), total fat (4.5±0.38 %) and ascorbic acid (68.5±0.19mg/100g). The hydroalcoholic extract showed the highest quantity of total phenol, total flavonoids, and total tannin content compared to aqueous extract. The hydroalcoholic extract exhibited high DPPH radical scavenging activity (IC50=68.1±0.34 µg/ml). The result showed that A.glauca roots have high nutritional and antioxidant potential. Hence the plant can be used as a nutraceutical and natural antioxidant.

scholarly journals Phytochemical Profiling, Antioxidant and Antibacterial Activities of Leaf Extracts From Rhus leptodictya

2017 ◽  
Vol 9 (08) ◽  
Author(s):  
Fanyana M. Mtunzi ◽  
Ikechukwu P. Ejidike ◽  
Tshifhiwa Matamela ◽  
Ezekiel Dikio ◽  
Michael J. Klink
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Focal Point ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Active Compounds

An integral part of nature is herbal plants which contain natural substance essential to promote health. The focal point of this research resides on the phytochemical, antioxidant and antibacterial investigations of Rhus leptoditya leaves. Hexane, dichloromethane, ethyl acetate, acetone, and methanol solvent extracts were tested for their antioxidant and antimicrobial properties. Phytochemical analysis of R. leptodictya extracts revealed the presence of phenols, tannins, saponins, and flavonoids. Acetone extract with total flavonoid contents of 18.22 ± 2.1 mg/g while methanol extract with phenol contents (0.38 ± 0.002 mg/g) exhibited the highest amount amongst the studied extracts in the preliminary phytochemicals screening. The antioxidant activity of the plant extracts was evaluated using TLC based standard qualitative 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The methanol and acetone extracts showed maximum antioxidant bands compared to other selected solvents and promising radical scavenging activity at par with the standard antioxidant ascorbic acid (IC50 = 0.125 ± 0.013 mg/ml). The bioautography assay also demonstrated the bacterial growth inhibition of active compounds present in leave extracts. Acetone and ethyl acetate fractions showed the highest number of antibacterial active compounds against S. aureus. Minimum inhibitory concentration of the extracts examined using microplates of 96 wells containing Muller-Hinton methods against some bacterial strains, revealed methanol and acetone fractions with the lowest MIC value of 0.04 mg/ml against S. pneumoniae, while methanol extract possesses 0.008 mg/ml against S. aureus and B. subtilis. Results from this study project the R. leptodictya leave extracts constituents as health supplements that could be useful in food industries and in the treatment of various infectious diseases.

scholarly journals PHYTOCHEMICALS AND ANTIOXIDANT ACTIVITY OF CLERODENDRUM PANICULATUM (L.) LEAF AND FLOWER EXTRACTS

2021 ◽  
pp. 31-34
Author(s):  
DEEPA R HEBBAR ◽  
DEVIKA M ◽  
RASHMI GN ◽  
NALINI MS
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Hexane Extracts ◽  
Reducing Power Assay

Objective: This study was designed to evaluate the phytochemicals present in the flower and leaf extracts of Clerodendrum paniculatum L., collected from Nelji village of Kodagu district . Methods: The healthy leaves and flowers of C. paniculatum were collected and the plant extracts were prepared using ethanol, hexane and distilled water separately. Phytochemical analysis was conducted using standard procedurs for the flower and leaf extracts of C. paniculatum. The antioxidant activity in leaf and flower extracts was determined by three assays, estimation of total phenolic content, reducing power assay and radical scavenging activity (DPPH) using standard procedures. Results: Phytochemical screening conducted for the flower and leaf extracts of C. paniculatum showed the presence of three phytochemicals, namely saponins, alkaloids and terpenoids. Terpenoids were commonly present in all the extracts of flower and leaf that is in both polar (aqueous and ethanol) solvent and in non-polar (hexane) solvents. The extracts tested for the antioxidant activity showed the presence of total phenolics in ethanol, aqueous and hexane extracts. The aqueous extract showed high redox potential followed by ethanol and hexane extracts. The aqueous leaf extract showed high radical scavenging activity when compared to the flower extracts of C. paniculatum. Conclusion:  The present study showes C. paniculatum to be an important medicinal plant, since the flower and leaves showed good antioxidant activity. Thus it may used in the treatment of diseases and may also used in the preparation of natural or herbal drugs due to the presence of antioxidants.

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