Bactericidal and Fungicidal Activity of Polyetherguanidinium Chloride

2021 ◽  
Vol 83 (1) ◽  
pp. 49-57
Author(s):  
M.Ya. Vortman ◽  
◽  
Yu.B. Pysmenna ◽  
A.I. Chuenko ◽  
A.V. Rudenko ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Fungicidal Activity ◽  
Heterotrophic Bacteria ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Microscopic Fungi ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Wide Range

There is information in the literature about the salts of polyhexamethylene guanidine (PGMG), which are effective biocidal and sterilizing drugs and disinfectants due to the wide range of their antimicrobial activity against gram-positive and gram-negative bacteria (including Mycobacterium tuberculosis), viruses, and fungi. The aim of this work is to study the bactericidal and fungicidal activity of the synthesized polyetherguanidinium chloride against a number of bacteria and microscopic fungi. Methods. Cultivation of microorganisms. Bacteria were grown on meat-peptone agar for 48 hours at a temperature of 28±2°C. Test cultures of micromycetes were cultured on beer wort agar (6°B), incubated for 14 days in a thermostat at a temperature of 28±2°C. Antimicrobial activity of newly synthesized polyetherguanidinium chloride was determined by standard disco-diffusion method, and fungicidal activity was determined by agar diffusion method. Results. The synthesis of polyetherguanidinium chloride was carried out in two stages. The first stage was the synthesis of a guanidinium-containing oligoether with terminal guanidine moieties by the reaction between an aromatic oligoepoxide and guanidine. The second stage was the synthesis of polyetherguanidinium chloride by the reaction between a guanidinium-containing oligoether with terminal guanidine moieties and oligooxyethylenediamine. The bactericidal and fungicidal activity of polyetherguanidinium chloride against various heterotrophic bacteria and microscopic fungi has been shown. It was found that polyetherguanidinium chloride at concentrations of 1–3% inhibited the growth of gram-negative (Escherichia coli 475, Klebsiella pneumonia 479) and gram-positive (Staphylococcus aureus 451) bacteria. The proposed 1% solution of polyetherguanidinium chloride shows a 1.5 times higher antimicrobial activity than the polymeric disinfectant polyhexamethyleneguanidinium chloride for E. coli 475 and K. pneumoniae 479 bacteria and lower antimicrobial activity for S. aureus 451 bacteria. According to the obtained data, it was noted that polyetherguanidinium chloride at a concentration of 1% had a high fungicidal activity against almost all investigated isolates: Aspergillus versicolor F-41250, Acremoneum humicola F-41252, Acremoneum roseum F-41251, Cladosporium sphaerospermum F-41255, Paecilomyces lilacinus F-41256 and Scopulariopsis candida F-41257. Conclusions. Received polyetherguanidinium chloride at a concentration of 1% showed bactericidal activity against S. aureus 451, E. coli 475, K. pneumoniae 479 and fungicidal effect to all fungi studied by us, and so can be used as a disinfectant for building materials.

Antimicrobial activity of Pycnarrhena cauliflora (Miers.) Diels. methanol extract

2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Eti Nurwening Sholikhah ◽  
Maulina Diah ◽  
Mustofa ◽  
Masriani ◽  
Susi Iravati ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts

Pycnarrhena cauliflora (Miers.) Diels., local name sengkubak, is one of indigenous plants from West Kalimantan that has been used as natural flavor. Pycnorrhena cauliflora is one of species of Menispermaceae family which is rich in bisbenzylisoquinoline alkaloids. This alkaloids are known to have various biological activities including antiprotozoal, antiplasmodial, antifungal and antibacterial activities. This study aimed to investigate antimicrobial activity of  the P. cauliflora (Miers.) Diels. methanolic extracts against gram-positive and gram-negative bacteria. The methanolic extract of P. cauliflora (Miers.) Diels., root, leaf and stem were prepared by maceration. The disk-diffusion method was then used to determine the antimicrobial activity of the extracts against Streptococcus pyogenes, S. mutants, Staphylococcus aureus, S. epidermidis, Salmonella typhi, Shigella flexneri, Pseudomonas aeruginosa and Escherichia coli after 18-24 h incubation at 37 oC. Amoxicillin was used as positive control for gram-positive bacteria and ciprofloxacin was used as gram-negative bacteria. The inhibition zones were then measured in mm. Analysis were conducted in duplicates. The results showed in general the methanolic extracts of P. cauliflora (Miers.) Diels. root (inhibition zone diameter= 10-23 mm) were more active than that leaf (0-15 mm) and stem (0-17 mm) extracts against gram-positive bacteria. The zone inhibition diameter of amoxicillin as positive control was 8-42 mm. In addition, the methanolic extracts of P. cauliflora (Miers.) Diels. root (12-17 mm) were also more active than that leaf (0-12 mm) and stem (0-12 mm) extracts against gram-negative bacteria. The zone inhibition diameter of ciprofloxacin as positive control was 33-36 mm. In conclusion, the methanolic extract of P. caulifloria (Miers.) Diels. root is the most extract active against both gram-positive and gram-negative bacteria. Further study will be focused to isolate active compounds in the methanolic extract of the root.

scholarly journals IN VITRO ANTIMICROBIAL ACTIVITY OF ROOT EXTRACT OF CLITORIA TERNATEA

2017 ◽  
Vol 10 (11) ◽  
pp. 52
Author(s):  
Amita Shobha Rao ◽  
Shobha Kl ◽  
Prathibha Md’almeida ◽  
Kiranmai S Rai
Keyword(s):  
Antimicrobial Activity ◽  
Resistant Strain ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Root Extract ◽  
Alcoholic Extract ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
E Coli ◽  
Multidrug Resistant Strain

  Objective: Infections caused by Gram-negative bacteria are important causes of morbidity and mortality. Extracts of plants and herbs such as Clitorea ternatea are used as diuretic. This work attempts to find out antimicrobial activity of aqueous and alcoholic extract of C. ternatea roots against Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), clinical strains of Klebsiella pneumoniae, and Candida albicans.Methods: The agar well-diffusion method was done using Mueller Hinton agar and Sabouraud’s dextrose agar. The microorganism grown in peptone water was inoculated into culture medium. 4 mm diameter well punched into the agar was filled with 20 μl of aqueous and alcoholic root extracts C. ternatea extracts in various concentrations (100-25 μg/ml). The plates were incubated and antimicrobial activity was evaluated.Results: Aqueous root extract of C. ternatea with the concentration of 100 μg/ml showed zone of inhibition against E. coli (ATCC 25922) 18 mm, P. aeruginosa (ATCC 27853) 14 mm, multidrug resistant strain of K. pneumoniae 15 mm. Alcoholic extract of C. ternatea with the concentration of 100 μg/ml showed zone of inhibition of 35 mm against E. coli (ATCC 25922), P. aeruginosa (ATCC 27853) 22 mm, and multidrug resistant strain of K. pneumoniae 28 mm. C. albicanswas resistant to both extract of C. ternatea root. Conclusions: Alcoholic extract of C. ternatea is a better antibacterial agent against multidrug resistant Klebsiella species and other Gram-negative pathogens. Further, studies are required to identify active substances from the alcoholic extracts of C. ternatea for treating infections.

Study of the chemical composition, antimicrobial activity of the essen- al oil of Cistus salviifolius from Tissemsilt National Park (Algeria) and influence of the drying period in the shade on the yield of this oil

2020 ◽  
Vol 9 (6) ◽  
pp. 238-244
Author(s):  
Meharrar Nadjet ◽  
Bekada Ahmed Mohamed Ali ◽  
Dris Ibrahim ◽  
Jacques Dommes ◽  
Arabi Abed ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Wide Spectrum ◽  
Diffusion Method ◽  
Bacterial Suspension ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Antibiotic Susceptibility Test ◽  
Cistus Salviifolius

The genera Cistus present groups of about twenty species of shrubs belon- ging to the Cistaceae family are very common throughout the Mediterranean region. Cistus salviifolius, known as Melliya is a species of this genus that occurs in the forest region of Mount Ouarsenis (northwest Algeria), exactly in Thniet El Had Na onal Park in the Wilaya of Tissemsilt. This study was un- dertaken to evaluate the an bacterial effects of C. salviifolius and its chemical composition in addition the effect of shade drying me on essen al oil yield in order to op mize this parameter which is most o en not controlled for producers. The op mal yield recorded is about 0.07± 0.02%. Its characte- riza on by gas chromatography coupled with mass spectrometry shows a dominance of hydrocarbon compounds. The minimum inhibitory concentration (MIC) is established using 6 strains of Gram-positive and negative bacteria. The antibiotic susceptibility test is performed using the giliose- environment diffusion method, a bacterial suspension is prepared and subsequently well homogenized. Opacity must be adjusted to 0.5 McF (McFarland), from a young exponen al culture (18 to 24 hours). The an mi- crobial ac vity of C. salviifolius EO was tested against three Gram-posi ve and three Gram-nega ve bacteria. In fact, a strong inhibitory and bactericidal effect has been demonstrated against all Gram-positive and Gram- negative bacteria tested. It appears from this study that the EO of C. salviifo- lius has a very high antimicrobial activity, it shows a wide spectrum covering Gram positive and Gram negative bacteria. Taking into account the results of this study, new an bacterial agents could be developed and the use of C.salviifolius should be encouraged in the pharmaceutical and perfumery industries.

Antioxidant and Antimicrobial Activities of the Fresh aerial parts of Ammi visnaga L. from Algeria

2016 ◽  
Vol 6 (2) ◽  
pp. 70-76
Author(s):  
Soumia Keddari ◽  
Narimen Benaoum ◽  
Yasmina Mokhtaria Boufadi ◽  
Mansouria Belhocine ◽  
Ali Riazi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Ammi Visnaga ◽  
Wide Range

Medicinal plants have been used for countries as cures for human diseases because they contain components of therapeutic value. Among these medi-cinal plants, Ammi visnage which have an immense reservoir of potential compounds attributed to the secondary metabolites which have the advan-tage of being of great diversity of chemical structure and have a very wide range of biological activities. The objectives of the present work were to stu-dy the antioxidant and antimicrobial activity of phenolic compounds ex-tracted from A. visnaga L. Its extraction is performed by two methods, etha-nol extraction and water extraction. The results showed that A. visnaga L.. ethanolic extract contains a mixture of phytochemical classes as polyphenol, flavonoids and revealed that this plant has high antioxidant activity (IC50 0.069 mg/ml). Regarding the antimicrobial activity results expressed by the diameter of the inhibition zones by diffusion method AWDT, the most signifi-cant inhibition was observed against to Staphylococcus aureus (12 mm) to the ethanol extract at concentration of 100mg / ml. Thus the aqueous ex-tract had a significant inhibitory activity against on the strains Staphylococ-cus aureus (8 mm), E. coli ATCC 10536 (8 mm) to a concentration of 100 mg / ml. The results for the antibacterial properties have shown that Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and M. luteus.) were more sensitive than gram-negative (Pseudomonas aeruginosa, E. coli ATCC 10536) against from the action of phenolic compounds of the Ammi visnaga ethanolic extract.

scholarly journals Synthesis and Antimicrobial Activity of Piperine Analogues Containing 1,2,4-Triazole Ring

2019 ◽  
Vol 31 (5) ◽  
pp. 1077-1080
Author(s):  
Kottakki Naveen Kumar ◽  
Karteek Rao Amperayani ◽  
V. Ravi Sankar Ummdi ◽  
Uma Devi Parimi
Keyword(s):  
Antimicrobial Activity ◽  
Triazole Ring ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Antibacterial Study ◽  
Gram Negative ◽  
E Coli ◽  
Micro Organisms

A series 1,2,4-triazole piperine analogues (TP1-TP6) were designed and synthesized. The structures were confirmed using 1H NMR and 13C NMR. Antibacterial study was done using Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative micro-organisms (E. coli and Pseudomonas aeruginosa) by disc diffusion method. Compound containing chloro substitution (TP6) showed the highest effect, while compound TP1, TP3, TP4, TP5 showed the moderate activity.

Antimicrobial Activity of Photoactive Cerium Doped Zinc Oxide

2020 ◽  
Vol 307 ◽  
pp. 217-222 ◽  
Author(s):  
Maishara Syazrinni Rooshde ◽  
Wan Rafizah Wan Abdullah ◽  
Amie Zaidah Amran ◽  
Noradhiha Farahin Ibrahim ◽  
Fazilah Ariffin ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Uv Light ◽  
Light Exposure ◽  
Antimicrobial Properties ◽  
Diffusion Method ◽  
Gram Positive ◽  
E Coli ◽  
Antifouling Agent ◽  
Doped Zno ◽  
Zno Powder

Biofouling and biofilms exist as ubiquitous, undesirable accumulation of flora and fauna upon a given substrate when being immersed into an aquatic medium. Therefore, a novel antifouling based materials with the incorporation of nanotechnology has been developed for the prevention of biofouling in its initial stage through photocatalytic treatment. This study investigated the antimicrobial properties of photoactive Cerium (Ce) doped ZnO powder and explores its potential properties for future antifouling application. ZnO nanoparticles was doped with 0.4 mol% Ce was synthesized through the combination of modified citrate gelation technique and solid state sintering. The successful preparation of Ce doped ZnO was confirmed by XRD and SEM. The antimicrobial activity of Ce doped ZnO against E. coli and S. aureus was determined through antibacterial susceptibility test by agar well diffusion method whilst its photocatalytic inactivation efficiency against selected bacteria was analysed through photodegradation testing under UV light irradiation. The findings demonstrated that the synthesized Ce doped ZnO powder exhibited antibacterial effect against Gram-positive bacteria (S. aureus) and excellent photocatalytic efficiency to inactivate both Gram-negative (E. coli) and Gram-positive (S. aureus). 2 g/L of Ce doped ZnO catalyzed the 100% disinfection of both bacteria in 180 min of UV light exposure. Thus, this proved that Ce doped ZnO powder has the potential as efficient antifouling agent.

scholarly journals Antimicrobial Activity and HPLC Fingerprinting of CrudeOcimumExtracts

2011 ◽  
Vol 8 (3) ◽  
pp. 1430-1437 ◽  
Author(s):  
S. S. Deo ◽  
F. Inam ◽  
R. P. Mahashabde
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Ocimum Sanctum ◽  
Moderate Activity ◽  
Aqueous Extracts ◽  
Gram Positive ◽  
Strong Activity ◽  
Gram Negative ◽  
E Coli ◽  
Strong Antimicrobial Activity

The antimicrobial activity of crude methanolic and aqueous extracts ofOcimum sanctumandOcimum kilimandsacharicumagainst gram positive, gram negative and antifungal activity was evaluated to find the zone of inhibition and to set a HPLC profile or fingerprint of these extracts. The crude methanolic extract ofOcimum sanctumshowed strong antimicrobial activity againstS.aureusandC. albicansand moderate activity againstE. coliandB. subtilis. The crude methanolic extract ofOcimum kilimandsacharicumshowed strong antimicrobial activity againstS. aureus, E. coliandC. albicansat higher concentration, same as that shown by the standard forC. albicans. It showed moderate activity againstB. subtilis. The crude aqueous extracts of Ocimum sanctum showed strong antimicrobial activity againstS.aureusand moderate against others. Whereas the crude aqueous extracts ofOcimum kilimandsacharicumshowed moderate activity against the gram positive and gram negative organisms and strong activity againstC. albicansat higher concentration, same as that shown by the standard forC. albicans.

scholarly journals Synthesis of Plant-Mediated Silver Nanoparticles UsingDioscorea batatasRhizome Extract and Evaluation of Their Antimicrobial Activities

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
P. C. Nagajyothi ◽  
K. D. Lee
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Room Temperature ◽  
Antimicrobial Activities ◽  
Gram Positive ◽  
Gram Negative ◽  
Synthesis Of Nanoparticles ◽  
E Coli ◽  
Synthesize Silver Nanoparticles ◽  
Dioscorea Batatas

The eco-friendly synthesis of nanoparticles through various biological means helps to explore various plants for their ability to synthesize silver nanoparticles (AgNPs). Here we have synthesized AgNPs by using rhizome extract ofDioscorea batatasat as well as room temperature (). AgNPs were characterized under UV-vis spectrophotometer, SEM, FTIR, XRD, and EDX. The antimicrobial activity of AgNPs was evaluated on gram positive (B. substilisandS. aureus), gram negative (E. coli), and fungi (S. cerivisaeandC. albicans). At room temperature,S. cerivisaeandC. albicanswere found to be more susceptible to AgNPs than at .

scholarly journals Antimicrobial activity of methanol extracts of four Parmeliaceae lichen species

2013 ◽  
Vol 11 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Igor Stojanovic ◽  
Niko Radulovic ◽  
Vladimir Cvetkovic ◽  
Tatjana Mitrovic ◽  
Slavisa Stamenkovic
Keyword(s):  
Antimicrobial Activity ◽  
Statistical Treatment ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Methanol Extracts ◽  
Group I ◽  
Distinguished Group

Antimicrobial activity of methanol extracts of four Parmeliaceae lichens (Hypogymnia physodes (L.) Nyl., Evernia prunastri (L.) Ach., Flavoparmelia caperata (L.) Hale and Parmelia sulcata Taylor) against a panel of microbial strains (11 Gram-positive (Enterococus sp., Bacillus subtilllis, Sarcina lutea, Micrococus luteus, Staphylococcus aureus, Clostridium sporogenes) and Gram-negative bacteria (Escherichia coli, Proteus vulgaris, Salmonela enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae), the filamentous fungus A. niger and the yeast C. albicans) was assayed using a disk diffusion method (1 mg of the extract per disc; extracts were dissolved in methanol, 25 mg/mL). All tested extracts showed moderate antimicrobial activity. Multivariate statistical treatment (agglomerative hierarchical clustering analysis, AHC) of the obtained results allowed grouping of the samples according to their antimicrobial potential against different strains: antimicrobial profile of H. physodes and E. prunastri extracts were comparable; the similar is true for F. caperata and P. sulcata samples. In addition, based on the similarities/ dissimilarities in their susceptibility toward the tested extracts, two groups of microorganisms could be distinguished: Group I - P. vulgaris, K. pneumoniae (Gram-negative bacteria), A. niger and C. albicans; Group II - E. coli, S. enteritidis, P. aeruginosa (Gram-negative bacteria) and all of the assayed Gram-positive strains.

scholarly journals Hybrid Approach for Synthesis and Antimicrobial Activity of Heterocyclic Compounds

2018 ◽  
Vol 77 ◽  
pp. 35-52 ◽  
Author(s):  
Nisheeth C. Desai ◽  
Darshita V. Vaja
Keyword(s):  
Antimicrobial Activity ◽  
Heterocyclic Compounds ◽  
Hybrid Approach ◽  
Spectroscopic Techniques ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Microbial Strains

We have synthesized novel series of N-(1-(2-(1-phenyl-3-(p-tolyl)-1H-pyrazol-4-yl)-5-(pyridin-4-yl)-1,3,4-oxadiazol-3(2H)-yl)ethylidene)arylaniline and their derivatives. The structures of synthesized compounds were well characterized by spectroscopic techniques. Antimicrobial activity of the newly synthesized derivatives was evaluated against gram positive (S. aureus and S. pyogenes), gram negative bacteria (E. coli and P. aeruginosa), and strains of fungi (C. albicans, A. niger and A. clavatus). Among the screened derivatives 5c, 5f, 5i, 5l and 5t demonstrated superior antimicrobial activity against microbial strains.

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