scholarly journals THE EFFECT OF ZnSO4 IN THE CULTIVATION MEDIUM ON RHODOTORULA GLUTINIS CCY 020-002-033 YEAST BIOMASS GROWTH, ß-CAROTENE PRODUCTION AND ZINC ACCUMULATION

2018 ◽  
Vol 8 (3) ◽  
pp. 931-935
Keyword(s):  
Rhodotorula Glutinis ◽  
Biomass Growth ◽  
Cultivation Medium ◽  
Zinc Accumulation ◽  
Yeast Biomass

Integrative platform for the selective recovery of intracellular carotenoids and lipids from Rhodotorula glutinis CCT-2186 yeast using mixtures of bio-based solvents

2020 ◽  
Vol 22 (23) ◽  
pp. 8478-8494
Author(s):  
Cassamo U. Mussagy ◽  
Valéria C. Santos-Ebinuma ◽  
Kiki A. Kurnia ◽  
Ana C. R. V. Dias ◽  
Pedro Carvalho ◽  
...  
Keyword(s):  
Rhodotorula Glutinis ◽  
Environmentally Benign ◽  
Selective Recovery ◽  
Yeast Biomass

Mixed biosolvents as a promising and environmentally benign solution for the recovery of carotenoids and lipids from yeast biomass.

scholarly journals Effect of Polysorbates on the Growth of Rhodotorula Glutinis in Oil Rich Medium

2021 ◽  
Vol 25 (1) ◽  
pp. 1075-1085
Author(s):  
Svetlana Raita ◽  
Kriss Spalvins ◽  
Evalds Raits ◽  
Inese Silicka ◽  
Dagnija Blumberga
Keyword(s):  
Oleaginous Yeast ◽  
Control Sample ◽  
Rhodotorula Glutinis ◽  
Inhibitory Effect ◽  
Tween 20 ◽  
Rich Medium ◽  
Yeast Growth ◽  
Thermogravimetric Method ◽  
Yeast Biomass ◽  
Medium Supplementation

Abstract The present study has investigated the effect of oil rich medium supplementation with polysorbates Tween 20, 40 and 80, for the cultivation of red oleaginous yeast Rhodotorula glutinis. R. glutinis has been cultivated in yeast extract peptone glucose modified broth (mYPG) supplemented with 2 % of waste cooking rapeseed oil and three polysorbate types with 0.5 %, 1 %, 2%, 3 %, 4 %, 5 %, 6 %, 7 %, 8 %, 9 % and 10 % concentration each. Yeast biomass was measured by the thermogravimetric method at 105 °C each day during 7-day experiment. The oil rich medium supplementation with Tween 20, 40 and 80 at concentrations ranging from 2 % to 10 % significantly increased the biomass of R. glutinis. All three types of the studied polysorbates with 0.5 % and 1 % concentration, did not affect yeast growth and the dry biomass – results were similar to the control sample without polysorbate addition. Between the three types of polysorbates, Tween 20 was selected as the preferable for R. glutinis cultivation with an optimal concentration of 2 %. Cultivation of R. glutinis in oil rich medium with polysorbates Tween 20, 40 and 80, supplementation up to 10 % concentration did not have had an inhibitory effect on the biomass growth.

scholarly journals Effect of Peg-Coated Silver and Gold Nanoparticles on Spirulina platensis Biomass during Its Growth in Closed System

2020 ◽  
Author(s):  
Liliana Cepoi ◽  
Inga Zinicovscaia ◽  
Ludmila Rudi ◽  
Tatiana Chiriac ◽  
Ion Rotari ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Polyethylene Glycol ◽  
Closed System ◽  
Industrial Application ◽  
Spirulina Platensis ◽  
Ultrastructural Changes ◽  
Biomass Growth ◽  
Cultivation Medium ◽  
Silver And Gold Nanoparticles ◽  
Promising Tool

Silver and gold nanoparticles are a promising tool for medical and industrial application, therefore, their ecotoxicity should be carefully examined. The effect of silver (12 nm) and gold (4.7) nanoparticles coated with polyethylene glycol on Spirulina platensis biomass growth and biochemical composition was investigated. The spirulina cultivation medium was supplemented with nanoparticles in concentration range 0.025-0.5 µM. Given concentrations stimulated spirulina biomass growth, while content of proteins, carbohydrates and auxiliary pigments was affected insignificantly by presence of nanoparticles in cultivation medium. The pronounced effect of gold nanoparticles at concentration 0.5 µM on the lipids content was observed. TEM images demonstrate that nanoparticles penetrate inside the cell, resulting in ultrastructural changes in cells.

Removal of Copper (II) Ions from Aqua Growth Medium by Red Yeast

2016 ◽  
Vol 851 ◽  
pp. 3-7
Author(s):  
Emília Breierova ◽  
Milan Čertík ◽  
Ivana Marova
Keyword(s):  
Cell Wall ◽  
Copper Ions ◽  
Rhodotorula Glutinis ◽  
Dry Weight ◽  
Cultivation Medium ◽  
Red Yeast ◽  
Sorption Ability ◽  
Sporobolomyces Roseus ◽  
High Sorption ◽  
Natural Fungicide

Copper is a natural fungicide and is the active component of various pesticides. We detected uptake of higher concentration of the copper ions and responses to this stress in combination with presence hydrogen peroxide as a source of free radicals were studied on the three red yeast strains of species Rhodotorula glutinis (two strains) and Sporobolomyces roseus (one strain). The maximum Cu sorption was observed at the cells of strain Rhodotorula glutinis CCY 20-2-33 (25,18 mg/g dry weight) and at their exopolymers which accumulated the amount 10.22 mg/g dry weight. The remaining copper was sorbed onto the fibrillar part of cell wall (3.75 mg/g dry weight). The presence peroxide (oxidative stress) in cultivation medium decreased of the toleration of yeasts to Cu2+ ions and cells were able to take up less of about 17 % (from 3mM on 2,5 mM), although total uptake was lower about 11.01-15.96 %. We found that the strains of Rhodotorula glutinis are able to uptake about 44 % more copper ions (25.18-24.32 mg/g dry weight) in compared with strain of Sporobolomyces roseus (16.92 dry weight). However, the addition of peroxide into the cultivation medium the addition of affecting trade changes by reduce of the ability to uptake Cu2+ ions. The exopolymers and fibrillar part of cell wall these yeast were used as biopolymers with high sorption ability for metals.

Yacon as a culture medium constituent to culture yeasts

2021 ◽  
pp. 58-60
Author(s):  
Валентина Батырбековна Цугкиева ◽  
Алан Макарович Хозиев ◽  
Борис Георгиевич Цугкиев ◽  
Лариса Батарбековна Дзантиева ◽  
Славик Урушалиевич Хаирбеков
Keyword(s):  
Culture Medium ◽  
Rhodotorula Glutinis ◽  
Yeast Biomass ◽  
Pichia Kluyveri ◽  
Research Institute

Авторами установлено, что корнеклубни якона могут использоваться эффективно в качестве компонента питательной среды для культивирования дрожжей. Выявлено, что добавление мальтозы в питательную среду с яконом не оказывает существенного влияния на накопление биомассы дрожжей Rhodotorula glutinis ВКПМ Y-3469 и Pichia kluyveri ВКПМ Y-4343 селекции НИИ биотехнологии Горского ГАУ. The authors found that yacon pips can be effectively used as a culture medium constituent to cultivate yeasts. It was found that the maltose supplements to the culture medium with yacon do not significantly affect the accumulation of yeast biomass Rhodotorula glutinis VKPM Y-3469, Pichia kluyveri VKPM Y-4343 selected by the Research Institute of Biotechnology, Gorsky State Agrarian University.

scholarly journals Characterization of Arthrospira sp (Spirulina) Biomass Growth in Hydroponic Waste Solution: A Review

2020 ◽  
Vol 3 (4) ◽  
pp. 354-358
Author(s):  
Yan Valdez Santos Rodrigues ◽  
Edna Dos Santos Almeida ◽  
Erika Durão Vieira
Keyword(s):  
Culture Medium ◽  
Synthetic Medium ◽  
Biomass Growth ◽  
Cultivation Medium ◽  
Waste Solution ◽  
Alternative Source ◽  
Potential Source ◽  
Alternative Culture ◽  
Reducing Costs

The use of Spirulina (Arthospira sp) as a food source has stood out for being a potential source of proteins, fatty acids, vitamins, and others. However, the cultivation medium for its production is relatively expensive. Then, an alternative culture medium is necessary for reducing costs. So, hydroponics drainage effluent, a solution rich in nutrients, represents an alternative source of substrate for microorganism cultivation. Thus, this work aimed to characterize the biomass of Arthospira sp grown in an alternative culture medium based on hydroponic effluent through a systematic review. We noted a trend to reduce biomass growth and bioactive compounds due to an excess of iron and copper in the effluent. The study concluded that it needs to supplement the residual medium with a synthetic medium for its use to obtain biomass for commercial utilization.  

scholarly journals Study on suitable conditions for obtaining zinc – enriched yeast biomass by strain Saccharomyces pastorianus strain CNTP 4054

2017 ◽  
Vol 33 (2S) ◽  
Author(s):  
Le Hong Quang ◽  
Nguyen Thi Trang ◽  
Le Duc Manh ◽  
Nguyen Thi Minh Khanh ◽  
Trinh Thanh Ha ◽  
...  
Keyword(s):  
Food Safety ◽  
Yeast Extract ◽  
Food Industry ◽  
Nutritional Value ◽  
Fermentation Conditions ◽  
Zinc Accumulation ◽  
Food Industries ◽  
Yeast Biomass ◽  
Saccharomyces Pastorianus ◽  
High Level

Yeast has long been considered to be one of the main subjects of the food industry. Yeast has the ability to absorb and assimilate zinc to creating a group of zinc – enriched yeast products, which are source of functional products with high nutritional value, ensuring food safety. In this study, we examined, selected some conditions that affected the uptake of zinc – enriched yeast biomass by Saccharomyces pastorianus CNTP 4054 with high level of zinc accumulation. This strain was from strains collection in the Center of Industrial Microbiology, in Food Industries Research Institute. Suitable fermentation conditions for zinc – enrich biomass by strain Saccharomyces pastorianus CNTP 4054 were glucose – 200g/L, yeast extract – 50g/L, ZnSO4 – 750mg/L, fermented at 30°C for 72 Hour, input value pH = 7.  

Increased zinc accumulation in mineralized osteosarcoma tissue

2016 ◽  
Author(s):  
Mirjam Rauwolf ◽  
Bernhard Pemmer ◽  
Andreas Roschger ◽  
Anna Turyanskaya ◽  
Rolf Simon ◽  
...  
Keyword(s):  
Zinc Accumulation

Investigation of culture conditions by Response Surface Methodology and kinetic modeling for exopolysaccharide production by Klebsiella oxytoca ICCF 419 strain, using lactose as substrate

2020 ◽  
Vol 25 (6) ◽  
pp. 2033-2044
Author(s):  
CATERINA TOMULESCU ◽  
◽  
MIŞU MOSCOVICI ◽  
ROXANA MĂDĂLINA STOICA ◽  
GABRIEL BUJOR ALBU ◽  
...  
Keyword(s):  
Citric Acid ◽  
Klebsiella Oxytoca ◽  
Fermentation Medium ◽  
Culture Conditions ◽  
Biomass Growth ◽  
Initial Value ◽  
Substrate Consumption ◽  
Polysaccharide Production ◽  
Crude Polysaccharide ◽  
Central Composite

The objective of this work was to optimize the bioprocess parameters, using Klebsiella oxytoca ICCF 419 to obtain an exopolysaccharide based on lactose as substrate. A kinetic study was employed and Logistic and Gompertz models were applied to describe the polysaccharide production, in relation with biomass growth and substrate consumption. The RSM methodology based on Central Composite Rotatable Design was used to evaluate and optimize the effect of lactose, corn extract, KH2PO4 and citric acid concentrations as independent variables on the polysaccharide production, biomass growth and substrate consumption as the response functions. The interaction effects and optimal parameters were obtained using Design Expert Software (version 9.0.6.2). The significance of the variables and their interactions was tested by means of ANOVA analysis with a 99% confidence level. The optimum culture conditions were determined and the model prediction was compared with experimental results. At an initial value of 23.45 for the C/N in the fermentation medium, the strain produces 17.41 g/L of crude polysaccharide and 2.53 g/L dry biomass. The EPS production was significantly influenced by lactose, corn extract and KH2PO4, while the citric acid had no influence. The biomass growth was influenced by the corn extract, KH2PO4 and citric acid.

Sign in / Sign up

Export Citation Format

Share Document