Removal of Copper (II) Ions from Aqua Growth Medium by Red Yeast

2016 ◽  
Vol 851 ◽  
pp. 3-7
Author(s):  
Emília Breierova ◽  
Milan Čertík ◽  
Ivana Marova
Keyword(s):  
Cell Wall ◽  
Copper Ions ◽  
Rhodotorula Glutinis ◽  
Dry Weight ◽  
Cultivation Medium ◽  
Red Yeast ◽  
Sorption Ability ◽  
Sporobolomyces Roseus ◽  
High Sorption ◽  
Natural Fungicide

Copper is a natural fungicide and is the active component of various pesticides. We detected uptake of higher concentration of the copper ions and responses to this stress in combination with presence hydrogen peroxide as a source of free radicals were studied on the three red yeast strains of species Rhodotorula glutinis (two strains) and Sporobolomyces roseus (one strain). The maximum Cu sorption was observed at the cells of strain Rhodotorula glutinis CCY 20-2-33 (25,18 mg/g dry weight) and at their exopolymers which accumulated the amount 10.22 mg/g dry weight. The remaining copper was sorbed onto the fibrillar part of cell wall (3.75 mg/g dry weight). The presence peroxide (oxidative stress) in cultivation medium decreased of the toleration of yeasts to Cu2+ ions and cells were able to take up less of about 17 % (from 3mM on 2,5 mM), although total uptake was lower about 11.01-15.96 %. We found that the strains of Rhodotorula glutinis are able to uptake about 44 % more copper ions (25.18-24.32 mg/g dry weight) in compared with strain of Sporobolomyces roseus (16.92 dry weight). However, the addition of peroxide into the cultivation medium the addition of affecting trade changes by reduce of the ability to uptake Cu2+ ions. The exopolymers and fibrillar part of cell wall these yeast were used as biopolymers with high sorption ability for metals.

scholarly journals Copper Ions Removal from Water using A2B3 Type Hyperbranched Poly(amidoamine) Hydrogel Particles

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3866
Author(s):  
Hojung Choi ◽  
Youngsik Eom ◽  
Sanghwa Lee ◽  
Sang Youl Kim
Keyword(s):  
Metal Ion ◽  
Copper Ions ◽  
Suspension Polymerization ◽  
Spherical Shape ◽  
Synthetic Process ◽  
Metal Ion Removal ◽  
Hyperbranched Poly ◽  
Sorption Ability ◽  
Heavy Metal Ion Removal ◽  
High Sorption

Micrometer-sized hyperbranched poly(amidoamine) (hPAMAM) particles are prepared with a simple A2B3 type Aza–Michael addition reaction between aminoethylpiperazine (AEP) and methylenebisacrylamide (MBA) in an inverse suspension polymerization condition. The synthesized particles exhibited surprisingly high Cu2+ sorption capacity (0.223g/g) for a solid-type absorbent. In addition to the high sorption ability of the particle, its simple synthetic process and convenience, due to its micrometer-sized spherical shape and recyclability, make it a practical and attractive absorbent for heavy metal ion removal from aqueous solutions.

scholarly journals Valorization of sugarcane bagasse by chemical pretreatment and enzyme mediated deconstruction

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Vihang S. Thite ◽  
Anuradha S. Nerurkar
Keyword(s):  
Cell Wall ◽  
Sugarcane Bagasse ◽  
Structural Changes ◽  
Enzymatic Saccharification ◽  
Dry Weight ◽  
Gravimetric Analysis ◽  
Chemical Pretreatment ◽  
Cell Wall Degrading Enzymes ◽  
First Time ◽  
Soluble Components

Abstract After chemical pretreatment, improved amenability of agrowaste biomass for enzymatic saccharification needs an understanding of the effect exerted by pretreatments on biomass for enzymatic deconstruction. In present studies, NaOH, NH4OH and H2SO4 pretreatments effectively changed visible morphology imparting distinct fibrous appearance to sugarcane bagasse (SCB). Filtrate analysis after NaOH, NH4OH and H2SO4 pretreatments yielded release of soluble reducing sugars (SRS) in range of ~0.17–0.44%, ~0.38–0.75% and ~2.9–8.4% respectively. Gravimetric analysis of pretreated SCB (PSCB) biomass also revealed dry weight loss in range of ~25.8–44.8%, ~11.1–16.0% and ~28.3–38.0% by the three pretreatments in the same order. Release of soluble components other than SRS, majorly reported to be soluble lignins, were observed highest for NaOH followed by H2SO4 and NH4OH pretreatments. Decrease or absence of peaks attributed to lignin and loosened fibrous appearance of biomass during FTIR and SEM studies respectively further corroborated with our observations of lignin removal. Application of commercial cellulase increased raw SCB saccharification from 1.93% to 38.84%, 25.56% and 9.61% after NaOH, H2SO4 and NH4OH pretreatments. Structural changes brought by cell wall degrading enzymes were first time shown visually confirming the cell wall disintegration under brightfield, darkfield and fluorescence microscopy. The microscopic evidence and saccharification results proved that the chemical treatment valorized the SCB by making it amenable for enzymatic saccharification.

scholarly journals Synthesis of Silver-Impregnated Magnetite Mesoporous Silica Composites for Removing Iodide in Aqueous Solution

Toxics ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 175
Author(s):  
Sang-Eun Jo ◽  
Jung-Weon Choi ◽  
Sang-June Choi
Keyword(s):  
Aqueous Solution ◽  
Langmuir Isotherm ◽  
High Surface ◽  
High Surface Area ◽  
High Capacity ◽  
Maximum Adsorption Capacity ◽  
Initial Contact ◽  
Order Kinetic ◽  
Sorption Ability ◽  
High Sorption

Mag@silica-Ag composite has a high sorption ability for I− in aqueous solution due to its high surface area and strong affinity for the studied anion. The material adsorbed I− rapidly during the initial contact time (in 45 min, η = 80%) and reached adsorption equilibrium after 2 h. Moreover, mag@silica-Ag proved to selectively remove I− from a mixture of Cl−, NO3− and I−. The adsorption behavior fitted the Langmuir isotherm perfectly and the pseudo-second-order kinetic model. Based on the Langmuir isotherm, the maximum adsorption capacity of mag@silica-Ag was 0.82 mmol/g, which is significantly higher than previously developed adsorbents. This study introduces a practical application of a high-capacity adsorbent in removing radioactive I− from wastewaters.

Phytotoxic Effects of Treated Wastewater Used for Agricultural Irrigation On Root Hydraulic Conductivity and Plant Growth

2021 ◽  
Author(s):  
Sare Asli ◽  
Nedal Massalha ◽  
Muhamad Hugerat
Keyword(s):  
Cell Wall ◽  
Hydraulic Conductivity ◽  
Plant Growth ◽  
Root Elongation ◽  
Dry Weight ◽  
Cell Permeability ◽  
Treated Wastewater ◽  
Pressurized Water ◽  
Pore Sizes ◽  
Physical Effects

Abstract AimsTo determine the effects of treated wastewater (TWW) and dialyzed TWW (DTWW) through dialysis tube with a cut-off at 6000-8000 Da, on the water transport characteristics of maize seedlings (Zea mays L). MethodsLaboratory experiments were conducted to determine the effect of TWW on the hydraulic conductivity of excised roots. Moreover, the effect on transpiration, plant growth, root cell permeability and on the plant fresh and dry weight was determined. ResultsPressurized water flow through the excised primary roots was reduced by 25%-52%, within 90 min of exposure to TWW or DTWW. In hydroponics, DTWW affected root elongation severely by 58 %, while cell-wall pore sizes of same roots were little reduced (by 6%). Additionally, the exposure to TWW or DTWW caused inhibition of both leaf growth rate by (26%-70%) and transpiration by (14%-64%). While in soil growth, the plant fresh and dry weight was also significantly affected but not with secondary DTWW. Conclusions These impacts appeared simultaneously to involve phytotoxic and physical clogging impacts. First, the inhibition in hydraulic conductivity through live roots (phytotoxic and physical effects) after exposure to secondary DTWW was by 22%, while through killed roots accepted after hot alcohol disruption of cell membranes (physical effects only); was only by 14%. Second, although DTWW affected root elongation severely by 58%, cell-wall pore sizes of same roots were little reduced by 6%. We conclude that large molecules, such as polypeptides, remained after the dialysis process, may have produced hormone-like activity that affected root water permeability.

scholarly journals Purification and Some Properties of Red Yeast Cell Wall Lytic Enzyme

1976 ◽  
Vol 40 (1) ◽  
pp. 27-32
Author(s):  
Motoo Arai ◽  
Ryoohei Yamamoto ◽  
Sawao Murao
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Yeast Cell Wall ◽  
Lytic Enzyme ◽  
Red Yeast

Fine structure in the red algae - II. The structure of the cell wall of Rhodymenia Palmata

1959 ◽  
Vol 150 (941) ◽  
pp. 447-455 ◽  
Keyword(s):  
Cell Wall ◽  
Chemical Treatment ◽  
Amorphous Matrix ◽  
Dry Weight ◽  
Wall Structure ◽  
X Ray Diffraction ◽  
X Ray ◽  
Floridean Starch ◽  
Thin Sectioning ◽  
Paper Chromatographic Separation

The cell-wall structure of the red alga Rhodymenia palmata has been examined by the methods of X -ray diffraction analysis and electron microscopy, including ultra-thin sectioning. The cell wall is shown to consist of numerous lamellae each of which is made up of unoriented, crystalline microfibrils embedded in an amorphous matrix of other cell-wall constituents. The material can be stretched reversibly up to 100% when wet, and the stretching induces orientation of the microfibrils. The ‘∝ cellulose' fraction, which accounts for only 2 to 7 % of the original dry weight, was isolated chemically and was analyzed by means of hydrolysis and paper chromatographic separation of the resulting sugars, and it was found to be composed of approximately equal quantities of glucose and xylose residues. Chemical treatment of the cell wall was found to cause considerable variations in the X -ray diagrams, which are discussed. It is concluded that the microfibrils contain both glucose and xylose residues in approximately equal proportions and that chemical treatment in this case causes changes in crystallinity of the structural component of the wall. The importance of these findings for the meaning of the term cellulose is discussed. The X -ray diagram of older fronds was found to be complicated by the occurrence of extra rings due to the presence of floridean starch, and the highly elastic properties of the thallus enabled the diagrams of the starch and the cell wall to be separated.

scholarly journals Cell Wall Saturation Limit and Selected Properties of Thermally Modified Oak Wood and Cellulose

Forests ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 640 ◽  
Author(s):  
Richard Hrčka ◽  
Viera Kučerová ◽  
Tatiana Hýrošová ◽  
Vladimír Hönig
Keyword(s):  
Molecular Weight ◽  
Cell Wall ◽  
Average Molecular Weight ◽  
Chemical Components ◽  
Water Molecules ◽  
Strong Negative Correlation ◽  
Oak Wood ◽  
Saturation Limit ◽  
Sorption Ability ◽  
Weighing Method

The interaction of water and oak wood is common in outdoor expositions and will remain a probable occurrence in the future. New insights into the recognition of a cell wall saturation limit are presented by a double-weighing method at 20 °C. The cell wall saturation limit, as the property of thermally modified oak wood, is significantly influenced by different treatment temperatures (20, 160, 180, 210 and 240 °C) on a 5% alpha level. A significantly higher equilibrium moisture content was reached by thermally modified oak wood at a temperature of 20 °C and relative humidity of 65% after its equilibrium in the water-in-reservoir. Moreover, the results are used in the treatment of woodchips to produce cellulose or decomposition of thermally modified wood to its basic chemical components. The investigated properties of cellulose revealed its relationship with water. The number of water molecules bonded to a cellulose chain was correlated with other measured compositions: average molecular weight, total crystalline index, lateral order index and polydispersity index. Analyses showed that there was a strong negative correlation between lateral order index and average molecular weight. The same was true between total crystalline index and average molecular weight. The rest of the properties were positively correlated with the number of water molecules bonded to glucopyranose. The results revealed the possible regeneration of a wood sorption ability after heat treatment and the stability of cellulose in such process.

Nutritional requirements for cell wall synthesis in Micrococcus lysodeikticus

1969 ◽  
Vol 15 (4) ◽  
pp. 327-334
Author(s):  
M. P. Hatton
Keyword(s):  
Amino Acids ◽  
Cell Wall ◽  
Glutamic Acid ◽  
Dry Weight ◽  
Defined Medium ◽  
Complete Medium ◽  
Magnesium Ions ◽  
Cell Wall Synthesis ◽  
Micrococcus Lysodeikticus ◽  
Total Dry Weight

Preferential cell wall synthesis in Micrococcus lysodeikticus, as determined by an increase in the dry weight of the cell wall, took place in a medium containing DL-glutamic acid, DL-alanine, L-lysine, glycine, magnesium ions, glucose and phosphate buffer, pH 7.0. Cell wall synthesis could not be completely dissociated from protein synthesis in the 'cell wall' medium. The cell wall synthesized in the defined medium accounted for 40–56% of the total dry weight increase of the cells. Chloramphenicol had no effect on cell wall synthesis. Incorporation of uracil and guanine in the medium did not result in any increase in the amount of cell wall synthesized. DL-Glutamic acid alone, or a mixture of the three amino acids DL-alanine, L-lysine, and glycine, were capable of replacing the four amino acids present in the complete medium, but under these conditions the total dry weight of cell wall synthesized was only 75% of that produced in the complete medium. There was no reduction in cell wall synthesis when L-glutamic acid replaced DL-glutamic acid, L-alanine replaced DL-alanine, or sucrose replaced glucose in the cell wall medium. Deprivation of magnesium ions produced the greatest decrease in wall synthesis; this was the most important single factor involved in cell wall synthesis which was studied in the present investigation. There was no observable change in the chemical composition of the cell wall synthesized in the 'wall' medium when compared to that synthesized by cells grown in a complex medium.

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