Characterization of Arthrospira sp (Spirulina) Biomass Growth in Hydroponic Waste Solution:  A Review

The use of Spirulina (Arthospira sp) as a food source has stood out for being a potential source of proteins, fatty acids, vitamins, and others. However, the cultivation medium for its production is relatively expensive. Then, an alternative culture medium is necessary for reducing costs. So, hydroponics drainage effluent, a solution rich in nutrients, represents an alternative source of substrate for microorganism cultivation. Thus, this work aimed to characterize the biomass of Arthospira sp grown in an alternative culture medium based on hydroponic effluent through a systematic review. We noted a trend to reduce biomass growth and bioactive compounds due to an excess of iron and copper in the effluent. The study concluded that it needs to supplement the residual medium with a synthetic medium for its use to obtain biomass for commercial utilization.

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Incorporation of micro/nanoparticles of PCL with essential oil of Cymbopogon nardus in bacterial cellulose

International Journal of Advances in Medical Biotechnology - IJAMB ◽

10.25061/2595-3931/ijamb/2018.v1i2.18 ◽

2018 ◽

Vol 1 (2) ◽

pp. 37

Author(s):

Aline Krindges ◽

Vanusca Dalosto Jahno ◽

Fernando Morisso

Keyword(s):

Particle Size ◽

Particulate Matter ◽

Essential Oil ◽

Zeta Potential ◽

Bacterial Cellulose ◽

Culture Medium ◽

Static Culture ◽

Cymbopogon Nardus ◽

Cellulose Membranes

Incorporation studies of particles in different substrates with herbal assets growing. The objective of this work was the preparation and characterization of micro/nanoparticles containing cymbopogon nardus essential oil; and the incorporation of them on bacterial cellulose. For the development of the membranes was used the static culture medium and for the preparation of micro/nanoparticles was used the nanoprecipitation methodology. The incorporation of micro/nanoparticles was performed on samples of bacterial cellulose in wet and dry form. For the characterization of micro/nanoparticles were carried out analysis of SEM, zeta potential and particle size. For the verification of the incorporation of particulate matter in cellulose, analyses were conducted of SEM and FTIR. The results showed that it is possible the production and incorporation of micro/nanoparticles containing essential oil in bacterial cellulose membranes in wet form with ethanol.

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A Setup for Microscopic Studies of Ultrasounds Effects on Microliters Scale Samples: Analytical, Numerical and Experimental Characterization

Pharmaceutics ◽

10.3390/pharmaceutics13060847 ◽

2021 ◽

Vol 13 (6) ◽

pp. 847

Author(s):

Florian N. Gailliègue ◽

Mindaugas Tamošiūnas ◽

Franck M. André ◽

Lluis M. Mir

Keyword(s):

Culture Medium ◽

Small Volume ◽

Acoustic Pressure ◽

Experimental Characterization ◽

Focal Distance ◽

Microscopic Studies ◽

Cell Membrane Permeabilization ◽

Ultrasonic Parameters ◽

Rigid Walls

Sonoporation is the process of cell membrane permeabilization, due to exposure to ultrasounds. There is a lack of consensus concerning the mechanisms of sonoporation: Understanding the mechanisms of sonoporation refines the choice of the ultrasonic parameters to be applied on the cells. Cells’ classical exposure systems to ultrasounds have several drawbacks, like the immersion of the cells in large volumes of liquid, the nonhomogeneous acoustic pressure in the large sample, and thus, the necessity for magnetic stirring to somehow homogenize the exposure of the cells. This article reports the development and characterization of a novel system allowing the exposure to ultrasounds of very small volumes and their observation under the microscope. The observation under a microscope imposes the exposure of cells and Giant Unilamellar Vesicles under an oblique incidence, as well as the very unusual presence of rigid walls limiting the sonicated volume. The advantages of this new setup are not only the use of a very small volume of cells culture medium/microbubbles (MB), but the presence of flat walls near the sonicated region that results in a more homogeneous ultrasonic pressure field, and thus, the control of the focal distance and the real exposure time. The setup presented here comprises the ability to survey the geometrical and dynamical aspects of the exposure of cells and MB to ultrasounds, if an ultrafast camera is used. Indeed, the setup thus fulfills all the requirements to apply ultrasounds conveniently, for accurate mechanistic experiments under an inverted fluorescence microscope, and it could have interesting applications in photoacoustic research.

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Detection and Characterization of Endothelin in Transformed Human Osteoblast Cell Culture Medium

Endocrine ◽

10.1385/endo:12:1:77 ◽

2000 ◽

Vol 12 (1) ◽

pp. 77-80 ◽

Cited By ~ 4

Author(s):

Hing-Chung Lam ◽

Jenn-Kuen Lee ◽

Kwok-Hung Lai

Keyword(s):

Cell Culture ◽

Culture Medium ◽

Human Osteoblast ◽

Osteoblast Cell ◽

Cell Culture Medium ◽

Human Osteoblast Cell

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Formulation of an Alternative Culture Medium forArthrospira maxima(Spirulina) Based on “Tequesquite,” a Traditional Mineral Resource

Journal of the World Aquaculture Society ◽

10.1111/jwas.12362 ◽

2016 ◽

Vol 48 (6) ◽

pp. 887-897 ◽

Cited By ~ 2

Author(s):

Fernando Martínez-Jerónimo ◽

Dante Ivo Flores-Hernández ◽

Juvencio Galindez-Mayer

Keyword(s):

Mineral Resource ◽

Culture Medium ◽

Alternative Culture

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Evaluation of Usefulness of the Designed Laboratory Photobioreactor for Microalgae Cultivation in Controlled Conditions

Agricultural Engineering ◽

10.1515/agriceng-2016-0002 ◽

2016 ◽

Vol 20 (1) ◽

pp. 13-22

Author(s):

Beata Brzychczyk ◽

Zbigniew Kowalczyk ◽

Jan Giełżecki

Keyword(s):

Culture Medium ◽

Dry Mass ◽

Biomass Growth ◽

Microalgae Cultivation ◽

Freshwater Microalgae ◽

Laboratory Conditions ◽

Medium Concentration ◽

Controlled Conditions ◽

The Impact

AbstractThe objective of the paper was to analyse the use of the designed photobioreactor for freshwater microalgae cultivation in the controlled laboratory conditions. The work covered the design and construction of photobioreactors (PBR) and setting up comparative cultivations of freshwater microalgae chlorelli vulgaris along with determination of the biomass growth intensity for a varied amount of supplied culture medium. It was found out that the constructed PBR may be used for microalgae cultivation in the controlled conditions. The impact of the culture medium amount on the growth of chlorelli vulgaris was proved. As a result of the increase of culture medium concentration to 30.1-120.4 ml·l−1 of water, dry mass in photobioreactorsincreased respectively from 1.33 g·dm−3 to 4.68 g·dm−3.

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Separation and Comparative Characterization of the Cationic Protease and Anionic Protease from the Culture Medium ofThermoactinomyces vulgaris

Hoppe-Seyler´s Zeitschrift für physiologische Chemie ◽

10.1515/bchm2.1982.363.2.843 ◽

1982 ◽

Vol 363 (2) ◽

pp. 843-854 ◽

Cited By ~ 10

Author(s):

Rolf KLEINE ◽

Ursula KETTMANN

Keyword(s):

Culture Medium ◽

Comparative Characterization

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Characterization of protein production by caprine placental membranes: identification and immunolocalization of retinol-binding protein

Journal of Endocrinology ◽

10.1677/joe.0.1460527 ◽

1995 ◽

Vol 146 (3) ◽

pp. 527-534 ◽

Cited By ~ 2

Author(s):

K H Liu ◽

J C Huang ◽

J D Godkin

Keyword(s):

Protein Production ◽

Culture Medium ◽

De Novo ◽

Binding Protein ◽

Yolk Sac ◽

Retinol Binding Protein ◽

Minimum Essential Medium ◽

Placental Membranes ◽

Extraembryonic Membranes

Abstract Caprine chorion, allantois and amnion from days 23, 28, 35, 39 and 45, and yolk sac from day 23 of pregnancy were isolated by dissection and cultured for 24 h in modified minimum essential medium in the presence of [35S] methionine. De novo-synthesized proteins released into the culture medium were analyzed by two-dimensional PAGE and fluorography. Patterns of protein production by these isolated extraembryonic membranes remained relatively unchanged from days 23 to 45 of pregnancy. Electrophoretic profiles of proteins synthesized by allantois and amnion were identical but distinct from that produced by chorion. Yolk sac was the major source of serum-like proteins. An acidic (pI 5·3–6·3) 22 kDa protein, which consisted of four isoelectric variants, was produced by all extraembryonic membranes and demonstrated to immunoreact with antiserum produced against bovine placental retinol-binding protein (RBP). Limited N-terminal sequence analysis of one major isoform indicated that the protein had complete homology with bovine RBP over the first 15 amino acids. Immunoreactive RBP was localized in epithelial cells lining the chorion, allantois and amnion. In this study, we have characterized and compared protein production by isolated extraembryonic membranes through days 23 to 45 of pregnancy and identified the 22 kDa protein as caprine RBP of placental origin. Journal of Endocrinology (1995) 146, 527–534

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Partial purification and characterization of an endo-α-N-acetylgalactosaminidase from the culture medium of Streptomyces sp. OH-11242

Biochemical Journal ◽

10.1042/bj2880475 ◽

1992 ◽

Vol 288 (2) ◽

pp. 475-482 ◽

Cited By ~ 30

Author(s):

I Ishii-Karakasa ◽

H Iwase ◽

K Hotta ◽

Y Tanaka ◽

S Omura

Keyword(s):

Enzyme Activity ◽

Culture Medium ◽

Gel Filtration ◽

Partial Purification ◽

Gastric Mucus ◽

Purification And Characterization ◽

Streptomyces Sp ◽

Optimum Ph ◽

New Type

For the purification of a new type of endo-alpha-N-acetylgalactosaminidase from the culture medium of Streptomyces sp. OH-11242 (endo-GalNAc-ase-S) [Iwase, Ishii, Ishihara, Tanaka, Omura & Hotta (1988) Biochem. Biophys. Res. Commun. 151, 422-428], a method for assaying enzyme activity was established. Using purified pig gastric mucus glycoprotein (PGM) as the substrate, oligosaccharides liberated from PGM were pyridylaminated, and the reducing terminal sugars of oligosaccharides larger than Gal beta 1-3GalNAc were analysed by h.p.1.c. The crude enzyme of endo-GalNAc-ase-S was prepared as an 80% (w/v) ammonium sulphate precipitate from the concentrated culture medium. The enzyme was partially purified by gel chromatofocusing and subsequent DEAE-Toyopearl chromatography. Endo-enzyme activity eluted around pI 4.8 on a gel chromatofocusing column and eluted with 0.19-0.25 M-NaCl on a DEAE-Toyopearl column. In the enzyme fraction obtained, no exo-glycosidases or proteases could be detected. The molecular mass of the enzyme was estimated as 105 kDa by gel filtration, and the optimum pH was 5.5. Endo-GalNAc-ase-S hydrolysed the O-glycosidic linkage between GalNAc and Ser (Thr) in 3H-labelled and unlabelled asialofetuin, liberating both the disaccharide (Gal beta 1-3GalNAc) and the tetrasaccharide [Gal beta 1-3 (Gal beta 1-4GlcNAc beta 1-6)GalNAc]. When endo-alpha-N-acetylgalactosaminidase from Alcaligenes sp. (endo-GalNac-ase-A) was incubated with 3H-labelled and unlabelled asialofetuin, only the disaccharide (Gal beta 1-3GalNAc) was liberated.

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