Apolipoprotein E gene polymorphism influenced glycemic status among Malaysians

Aim: In the last decade Apolipoprotein E (APOE) gene polymorphism has been identified as one of the risk factors of type 2 diabetes mellitus (T2DM). Though more than 11% population of Malaysia are suffering from T2DM, there is inadequate data on the correlation between the APOE gene polymorphism and pathogenesis of diabetes among Malaysians. Hence, in this study we aimed to find out the association between the frequencies of APOE allele and fasting blood glucose (FBG) concentration among subjects with T2DM. Methods: A total of 102 subjects were recruited into two distinct groups, 51 in diabetes (cases) and 51 in non-diabetes (control) group. Their fasting blood sample was tested for FBG, while APOE genotyping was carried out using restriction fragment length polymorphism technique. Predictive Analytics Software (PASW) statistics, version 18.0, was used for statistical analyses. Results: There was no association between APOE alleles and T2DM; odd ratios for the e2, e3 and e4 alleles were 1.51 (95%CI: 0.615-3.706), 0.77 (95%CI: 0.431-1.375) and 1.12 (95%CI: 0.584-2.131) respectively. The highest mean FBG was found in subjects with e2 alleles, followed by e4 and e3 alleles in both cases and control groups. Both e2 and e4 alleles were significantly linked to higher mean FBG (p=0.03 and 0.04 for the respectively) compared to e3 allele in diabetes group. Conclusions: Although the APOE gene was not found to be associated with T2DM, it may influence glycemic status among subjects.

Download Full-text

The Application Value of ApoE Gene Polymorphism in Alzheimer's Disease

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2019.2148 ◽

2019 ◽

Vol 9 (10) ◽

pp. 1403-1407

Author(s):

Cong Chen ◽

Yuhui Zhang ◽

Bin Chen ◽

Chaosheng Zeng ◽

Min Chen ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Gene Polymorphism ◽

Apoe Genotype ◽

Apoe Gene ◽

Control Group ◽

Apolipoprotein E Polymorphism ◽

Abnormal Lipid Metabolism ◽

And Control ◽

Apoe Gene Polymorphism

To explore the association of apolipoprotein E polymorphism with Alzheimer's disease (AD), so as to provide possible research value for potential targeted therapy. 120 AD patients and 50 healthy volunteers were enrolled to extract fasting blood samples. ApoE gene polymorphism and blood lipids were tested in blood. ApoE gene and genotype frequency between AD group and control group were compared by PCR and sequencing methods. MMSE, CDR, and BPSD were used to determine the intelligence. ApoE genotype was detected by DNA microarray. ɛ4 carrier accounted for 45% in AD group, which was significantly elevated compared with control group (12%) (P < 0.05). TG, TC, and LDL-C levels were increased, while HDL-C was reduced in ɛ4 allele carriers (allP < 0.05). The MMSE scores of ApoEɛ4 genotype carriers in AD group were markedly lower than those of nonApoEɛ4 genotype carriers (P < 0.05) and control (P < 0.01). The proportion of dementia in ApoEɛ4 genotype carriers from AD group was apparently higher than the ɛ4 gene non-carriers (P < 0.05). The ApoEɛ4 gene is an AD risk factor. The changes of genotype and frequency of ApoEɛ4 gene are the main factors leading to abnormal lipid metabolism in AD patients, suggesting that ApoEɛ4 gene detection might be helpful for the early diagnosis and treatment of AD.

Download Full-text

APOE E4 is associated with hyperlipidemia and obesity in elderly schizophrenic patients

Scientific Reports ◽

10.1038/s41598-021-94381-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Wei Li ◽

Fengju Liu ◽

Rui Liu ◽

Xinmei Zhou ◽

Guanjun Li ◽

...

Keyword(s):

Cognitive Function ◽

Gene Polymorphism ◽

Psychiatric Symptoms ◽

Linear Regression Analysis ◽

Fasting Blood Glucose ◽

Apoe Gene ◽

Significant Difference ◽

Schizophrenic Patients ◽

Apoe Gene Polymorphism ◽

Normal Controls

AbstractObesity is a critical issue in patients with schizophrenia, which is considered to be brought about by both environmental and genetic factors. Apolipoprotein E (APOE) gene polymorphisms might be involved in the pathogenesis of schizophrenia, however, the effect of APOE gene polymorphism on obesity has never been investigated in Chinese aging with schizophrenia. This cross-sectional study was to investigate the effect of obesity on cognitive and psychiatric symptoms in elderly participants with schizophrenia. At the same time, we also discussed the inner link between APOE E4 and obesity. 301 elderly participants with schizophrenia and 156 normal controls were included in the study. Their cognitive function was assessed using the Montreal Cognitive Assessment (MoCA), psychiatric symptoms were assessed using the Positive and Negative Syndrome Scale (PANSS), and APOE gene polymorphism was determined by polymerase chain reaction (PCR). The prevalence of obesity in elderly schizophrenic patients and healthy controls accounted for 15.9% (48/301) and 10.3% (16/156), respectively, with no statistically significant difference. By using stepwise linear regression analysis, we found that elevated fasting blood glucose, hypertension, and hyperlipidemia were risk factors for obesity in elderly schizophrenic patients. Although there was no direct correlation between APOE E4 and obesity in patients with schizophrenia, it was significantly correlated with hyperlipemia(r = − 0.154, p = 0.008), suggesting that APOE E4 may induce obesity in elderly patients with schizophrenia through hyperlipemia, However, the above conclusions do not apply to the normal elderly. What’s more, we did not find a link between obesity and cognitive function or mental symptoms for both patients with schizophrenia and normal controls. APOE E4 is associated with hyperlipidemia in elderly schizophrenic patients, which may be a risk factor for obesity, however, the above conclusion does not apply to the normal elderly.

Download Full-text

Effect of apolipoprotein E genotype on the outcome after anterior cervical decompression and fusion in patients with cervical spondylotic myelopathy

Journal of Neurosurgery Spine ◽

10.3171/2009.7.spine08667 ◽

2009 ◽

Vol 11 (6) ◽

pp. 659-666 ◽

Cited By ~ 19

Author(s):

Matthias Setzer ◽

Frank D. Vrionis ◽

Elvis J. Hermann ◽

Volker Seifert ◽

Gerhard Marquardt

Keyword(s):

Gene Polymorphism ◽

Apolipoprotein E ◽

Cervical Spondylotic Myelopathy ◽

Independent Predictor ◽

Multivariate Model ◽

Apoe Gene ◽

Categorical Variables ◽

Restriction Enzyme Digestion ◽

Microsurgical Decompression ◽

Apoe Gene Polymorphism

Object The authors examined a possible association between apolipoprotein E (APOE) gene polymorphism and the outcome after anterior microsurgical decompression in patients with cervical spondylotic myelopathy (CSM). Methods The authors conducted a prospective study of 60 consecutive patients (40 men, 20 women) with CSM who underwent anterior microsurgical decompression. The patients ranged in age from 26 to 86 years (mean 61.5 ± 14.6 years). Neurological deficits were classified according to the modified Japanese Orthopaedic Association Scale. Mean follow-up was 18.8 ± 4.6 months and APOE genotyping was carried out by isolation of DNA from venous blood samples. The APOE genotypes were determined by polymerase chain reaction followed by restriction enzyme digestion and polyacrylamide gel electrophoresis of digested fragments. Categorical variables were analyzed with the chi-square test, continuous data with the Mann-Whitney U-test, and for multiple groups with the Kruskal-Wallis H-test. A backward stepwise binary logistic regression analysis was performed to determine the effect of APOE in a multivariate model. Results Of the 60 patients with CSM, 35 (58.3%) improved and 25 (41.7%) did not improve or suffered deterioration (no-improvement group). In the improvement group 5 patients (8.3%) possessed the ε4 allele compared with 16 patients (26.7%) in the no-improvement group (p = 0.002, OR 3.3, 95% CI 1.7–6.1). In a multivariate model, the occurrence of the ε4 allele was a significant independent predictor for no improvement after anterior decompression and fusion (p = 0.004, OR 8.6, 95% CI 5.1–20.6). Conclusions The results of this study show that APOE gene polymorphism influences the short-term outcome of CSM patients after surgical decompressive and stabilizing therapy in the way that the presence of the APOE ε4 allele is an independent predictor for a no improvement. The presence of APOE may explain in part the different responses to operative therapies in patients with cervical myelopathy.

Download Full-text

Apolipoprotein E Gene Polymorphism, Glycated Hemoglobin, and Peripheral Arterial Disease Risk in Chinese Type 2 Diabetic Patients

Disease Markers ◽

10.1155/2020/6040525 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Yujing Hu ◽

Tinghuan Ling ◽

Min Yu ◽

Yang Bai ◽

Tongbao Feng ◽

...

Keyword(s):

Logistic Regression ◽

Peripheral Arterial Disease ◽

Gene Polymorphism ◽

Apolipoprotein E ◽

Arterial Disease ◽

Apoe Gene ◽

Diabetic Patients ◽

Peripheral Arterial ◽

Apoe Gene Polymorphism

Background. The apolipoprotein E (ApoE) gene polymorphism has been found to influence plasma lipid concentration, and its correlation with peripheral arterial disease (PAD) has been investigated. However, it is unclear whether ApoE is associated with PAD in Chinese type 2 diabetes mellitus (T2DM) patients. Therefore, our study is aimed at investigating the relationship between the ApoE gene polymorphism and PAD in Chinese T2DM patients. Methods. A total of 192 T2DM patients were divided into two groups: T2DM and T2DM with PAD. The clinical and biochemical parameters were obtained. Polymerase chain reaction was used to identify the genotypes of ApoE. The multivariable logistic regression analysis was used to identify the possible risk factor for PAD. Results. There were no significant differences in the genotype and allele frequencies of ApoE between the T2DM and T2DM with PAD groups. However, the T2DM with PAD group tended to have more ε4/ε3 genotypes (21.5% vs. 11.3%) than the T2DM group. The multivariate logistic regression showed that smoking, age, disease duration, TG, LDL, and HbA1c were risk factors for PAD. Conclusions. These results demonstrated that there was no evidence of a relationship between ApoE and PAD.

Download Full-text

Association of Apolipoprotein E Gene Polymorphism with Lipid Profile and Ischemic Stroke Risk in Type 2 Diabetes Mellitus Patients

Journal of Nutrition and Metabolism ◽

10.1155/2021/5527736 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Ni Putu Tesi Maratni ◽

Made Ratna Saraswati ◽

Ni Nyoman Ayu Dewi ◽

I Wayan Putu Sutirta Yasa ◽

I Putu Eka Widyadharma ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Gene Polymorphism ◽

Apolipoprotein E ◽

Density Lipoprotein ◽

Apoe Gene ◽

Apolipoprotein E Gene ◽

Apoe Gene Polymorphism

Background. Altered lipid profiles have consistently been linked to cerebrovascular events. Ischemic stroke (IS) was a common comorbid condition established in type 2 diabetes mellitus (T2DM). The apolipoprotein E (ApoE) gene which has a notably critical function in lipoprotein metabolism is believed as one of the potential candidate genes susceptible to IS complications in T2DM. This research aimed to determine the association of apolipoprotein E gene polymorphism with lipid profile and IS risk in T2DM patients. Methods. This case-control study involved a total of 60 diabetic participants divided into two groups with and without IS. ApoE was genotyped using PCR and sequencing analysis. Results. The most predominant genotype observed in 27 participants (45%) was E3/E3. Lower levels of high-density lipoprotein cholesterol (HDL-C) were found in ε2 carriers ( p = 0.003 ; 95% CI −23.35–−4.89) and ε4 carriers ( p = 0.019 ; 95% CI 1.38–14.55) compared to ε3 homozygotes. Total cholesterol (TC), triglyceride, and low-density lipoprotein cholesterol (LDL-C) levels had no association with ApoE gene polymorphism in this study. ApoE gene polymorphism was not related to IS in T2DM ( p = 0.06 ; adjusted OR: 4.71; 95% CI 0.93–23.79). Conclusions. ApoE ε2 and ε4 carriers were associated with lower levels of HDL-C. No association was identified between ApoE gene polymorphism and IS in T2DM patients.

Download Full-text

Complex adaptive systems and human health: the influence of common genotypes of the apolipoprotein E ( ApoE ) gene polymorphism and age on the relational order within a field of lipid metabolism traits

Human Genetics ◽

10.1007/s004390000394 ◽

2000 ◽

Vol 107 (5) ◽

pp. 466-475 ◽

Cited By ~ 23

Author(s):

Kim Zerba ◽

Robert Ferrell ◽

Charles Sing

Keyword(s):

Lipid Metabolism ◽

Gene Polymorphism ◽

Apolipoprotein E ◽

Human Health ◽

Complex Adaptive Systems ◽

Adaptive Systems ◽

Apoe Gene ◽

Complex Adaptive ◽

Relational Order ◽

Apoe Gene Polymorphism

Download Full-text

The Association between Apolipoprotein E Gene Polymorphism and Mild Cognitive Impairment among Different Ethnic Minority Groups in China

International Journal of Alzheimer s Disease ◽

10.1155/2014/150628 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

ZhiZhong Wang ◽

Wanrui Ma ◽

Ye Rong ◽

Lan Liu

Keyword(s):

Cognitive Impairment ◽

Mild Cognitive Impairment ◽

Gene Polymorphism ◽

Apolipoprotein E ◽

Ethnic Groups ◽

Apoe Genotype ◽

Melting Curve ◽

Apoe Gene ◽

Apoe Genotypes ◽

Apoe Gene Polymorphism

The association, in different ethnic groups, of apolipoprotein E (apoE) gene polymorphism with mild cognitive impairment (MCI) has been unclear. Few studies have examined the association in Chinese minorities. The current study explores the association between apoE gene polymorphism and MCI in one of the biggest ethnic groups—the Hui—and compares it with the Han. The Minimental State Exam, Activities of Daily Living Scale, and Geriatric Depression Scale were administered to 306 ethnic Hui and 618 ethnic Han people aged≥55 years. ApoE genotypes were determined using the high resolution melting curve method. The distribution of the apoE genotype and the frequency of allelesε2,ε3, andε4 were similar in the Hui and Han groups. In analyses adjusted for age, gender, and education level, theε4 allele was a risk factor for MCI in both the Hui group (OR=2.61, 95% CI: 1.02–6.66) and the Han group (OR=2.36, 95% CI: 1.19–4.67), but the apoEε2 allele was protective for MCI only in the Han group (OR=0.48, 95% CI: 0.38–0.88). The association of some apoE genotypes with MCI may differ in different ethnic groups in China. Further studies are needed to explore this effect among different populations.

Download Full-text

Role of Nutrition Counseling and Lifestyle Modification in Managing Prediabetes

Food and Nutrition Bulletin ◽

10.1177/03795721211025434 ◽

2021 ◽

pp. 037957212110254

Author(s):

Harleen Kaur ◽

Neerja Singla ◽

Rohini Jain

Keyword(s):

Hemoglobin A1c ◽

Lifestyle Modification ◽

Fasting Blood Glucose ◽

Nutrition Intervention ◽

Control Group ◽

Population Prevalence ◽

Significant Difference ◽

And Control ◽

Experimental Group

Objective: India is the second country after China having the highest population prevalence of diabetes. Several research studies investigating diabetes have been done, but not much work has been done on prediabetes. The purpose of this study was to investigate the effect of nutrition and lifestyle modification on prediabetic females. Methods: A total of 120 prediabetic females from Ludhiana city were divided into 2 matched groups: control group (n = 60) and experimental group (n = 60). Impact of nutrition intervention for dietary and lifestyle modification (for 3 months) was assessed on the anthropometric, dietary, biochemical parameters, and diabetes risk score of the experimental group and control group (no intervention). Results: All the selected 120 subjects completed the study (experimental group = 60; control group = 60). There was significant difference in the changes between the 2 groups throughout the study. The fasting blood glucose and glycated hemoglobin A1c levels of the experimental group subjects reduced significantly ( P ≤ .01). However, no change was observed among the control group subjects. The lipid profile of the experimental group showed a significant improvement ( P ≤ .01). Conclusion: Nutrition counselling of the prediabetics regarding dietary and lifestyle modification is recommended so as to improve their metabolic control, thus preventing them from being diabetics.

Download Full-text