scholarly journals The impact of humic acid additives on mineral metabolism of rabbits in the postnatal period of ontogenesis

2020 ◽  
Vol 11 (2) ◽  
pp. 289-293
Author(s):  
M. A. Rybalka ◽  
L. M. Stepchenko ◽  
O. O. Shuleshko ◽  
L. V. Zhorina
Keyword(s):  
Alkaline Phosphatase ◽  
Humic Acid ◽  
Blood Serum ◽  
Bone Tissue ◽  
Mineral Metabolism ◽  
Histological Study ◽  
Postnatal Period ◽  
Control Group ◽  
Copper And Zinc ◽  
Experimental Group

Humic acids are characterized by chelating properties due to which they are able to influence the mineral metabolism in animals. Rabbits have species-specific characteristics of mineral metabolism, which distinguishes them from most species of mammals. For the experiment, 16 rabbits of 45-day-old of Hyplus breed were divided into the experimental and control group of 8 rabbits in each. The animals were 15 days in the preparatory period. The rabbits of the experimental group received humic acid additive individually with water in the amount of 5 mg/kg, the rabbits of the control group received pure water in the same amount. The ability of humic acid additive to increase the content of calcium, ionized calcium, iron, to correct the content of copper and zinc, and also to increase the activity of alkaline phosphatase in the blood serum of rabbits of the experimental group has been proved. We observed the early effect of the humic acid additive on increasing the content of iron and calcium ionized and a later effect on the activity of alkaline phosphatase and an increase in the content of copper in the blood serum. The influence of humic acid additive on the distribution of calcium, phosphorus, manganese, copper and zinc in bone tissue is determined. According to a histological study, there was a significant increase in the number of layers of osteons and osteoblasts in the bone tissue of the femur, an increase in the number of osteons and osteoblasts in the bone tissue of the sternum and an increase in the number of columns of chondrocytes and an increase in the number of chondrocytes in the column in the cartilaginous tissue of the sternum. The results of a histological study of bone tissue, together with an increase in structural macroelements in it and redistribution of osteotropic trace elements in the blood and bone tissue suggest the possibility of using a humic acid additive to intensify the growth and mineralization of bone tissue, which can improve the state of bone tissue of rabbits in the postnatal period ontogenesis.

scholarly journals The impact of humic acid additives on mineral metabolism of rabbits in the postnatal period of ontogenesis

2020 ◽  
Vol 11 (2) ◽  
pp. 289-293
Author(s):  
M. A. Rybalka ◽  
L. M. Stepchenko ◽  
O. O. Shuleshko ◽  
L. V. Zhorina
Keyword(s):  
Alkaline Phosphatase ◽  
Humic Acid ◽  
Blood Serum ◽  
Bone Tissue ◽  
Mineral Metabolism ◽  
Histological Study ◽  
Postnatal Period ◽  
Control Group ◽  
Copper And Zinc ◽  
Experimental Group

Humic acids are characterized by chelating properties due to which they are able to influence the mineral metabolism in animals. Rabbits have species-specific characteristics of mineral metabolism, which distinguishes them from most species of mammals. For the experiment, 16 rabbits of 45-day-old of Hyplus breed were divided into the experimental and control group of 8 rabbits in each. The animals were 15 days in the preparatory period. The rabbits of the experimental group received humic acid additive individually with water in the amount of 5 mg/kg, the rabbits of the control group received pure water in the same amount. The ability of humic acid additive to increase the content of calcium, ionized calcium, iron, to correct the content of copper and zinc, and also to increase the activity of alkaline phosphatase in the blood serum of rabbits of the experimental group has been proved. We observed the early effect of the humic acid additive on increasing the content of iron and calcium ionized and a later effect on the activity of alkaline phosphatase and an increase in the content of copper in the blood serum. The influence of humic acid additive on the distribution of calcium, phosphorus, manganese, copper and zinc in bone tissue is determined. According to a histological study, there was a significant increase in the number of layers of osteons and osteoblasts in the bone tissue of the femur, an increase in the number of osteons and osteoblasts in the bone tissue of the sternum and an increase in the number of columns of chondrocytes and an increase in the number of chondrocytes in the column in the cartilaginous tissue of the sternum. The results of a histological study of bone tissue, together with an increase in structural macroelements in it and redistribution of osteotropic trace elements in the blood and bone tissue suggest the possibility of using a humic acid additive to intensify the growth and mineralization of bone tissue, which can improve the state of bone tissue of rabbits in the postnatal period ontogenesis.

scholarly journals Effects of biologically active substances on interior indicators of pigs in the early postnatal period

2019 ◽  
pp. 69-72
Author(s):  
Olga Nikolaevna Polozyuk ◽  
Tatyana Ivanovna Lapina
Keyword(s):  
Gastrointestinal Tract ◽  
Blood Serum ◽  
Dietary Supplements ◽  
Postnatal Period ◽  
Biologically Active ◽  
Control Group ◽  
Control Groups ◽  
And Control ◽  
Experimental Group ◽  
Active Substances

The authors found out that the application  of dietary supplements "Glimalask Lact" allowed preventing the disorder of the gastrointestinal tract and saving all piglets throughout the experiment.  After application of "Agrocid super oligo", the safety of purebred piglets was 100 %, and for two pedigree piglets it was 96.7%.   In piglets of the 1st and 2nd control groups, disorder of the gastrointestinal tract was noted during the completion of colostral immunity and at weaning from sows.  The safety of purebred piglets was 96.7%, and of the two pedigree piglets  it was 93.4%. The number of γg-globulins in the blood serum of two breed piglings treated with the Glimalask Lact supplement increased by 3.5 and 2.5% compared to the piglets of the experimental group fed with Agrocid super oligo, and of the control group, The number of g-globulins in the blood serum of purebred  piglets it increased by 2.4 and 3.1% compared with analogues of the experimental and control groups.

scholarly journals Influence of the supplement «Press-Acid» on protein-mineral metabolism and resistance of piglets

2018 ◽  
Vol 20 (83) ◽  
pp. 320-324 ◽  
Author(s):  
N.V. Chеrniy ◽  
E.V. Matsenko ◽  
Yu.A. Shchepetilnikov ◽  
Yu. V. Maslak ◽  
O.S. Machula ◽  
...  
Keyword(s):  
Blood Serum ◽  
Mineral Metabolism ◽  
The Body ◽  
Control Group ◽  
Immunological Parameters ◽  
Oxidative Reaction ◽  
Experimental Animals ◽  
Growth Energy ◽  
First Time ◽  
Experimental Group

The aim of this study was to determine the effect of supplement «Press-Acid» on some parameters of mineral and protein metabolism and resistance of piglets. The study was done in condition «Meta» on two groups of piglets of the F-1 (BWхL) genotype. There were determined indexes of bacterial and lysocim activity of blood serum, calcium and phosphorus in piglets at the age of 21–30–45–75 days. Effect of supplement on the growth rate, morbidity and safety of pigs were revealed. We used hygienic, zootechnical, hematological, biochemical, immunological and statistical methods of analysis. The influence of «Press-Acid», which consist of formic, lactic, citric and fumaric acids, was established for the first time. The concentration of calcium in blood serum of experimental group of piglets were lower than in animals of control group. The level of phosphorus increased in blood serum of experimental animals on 11.83% (30 day), on 3.74% (45%), on 17.65% (75 day) respectively. Concentration of hemoglobin and the amount of erythrocytes increased on 11.8% and on 1.82% (30 day) in animals that feed the supplement. There were established the increase of viability of the pigs with gastro-intestinal diseases which were given the supplement in comparison with that, who did not feed it. Severity of diseases was determined using the Melenberg coefficient which did not higher than 1.2–2.4 score, it was lower on 6,0–9,3 unit than in control group. Using the supplement allowed to increase the intensity of growth of piglets by 3.7% (45 day) and 9.4% (75 day) respectively. Concentration of total protein, albumines and globulins increased on 8.66%, 9.90%, 7.3% respectively. The activity of alaninaminotransphaerasae in the blood serum of experimental animals were lower on 3.45% than in control piglets. Lysocym and bacterial activity of blood serum were higher in experimental group of piglets. Feeding piglets with addition supplement Press Acid promotes higher grow rates in experimental animals, that can be explained increases of oxidative-reducing processes in them organism. Feeding piglets with acidifier «Press-Acid», «Nifulin», zinc oxide, probiotics «Lactisan Complex» promotes growth energy, increase of morphological, biochemical and immunological parameters which indicates a higher level of oxidative reaction in the body. A low coefficient of Melenberg is recorded in the piglets at the age of 1 day. Safety was within 92.1–97.0%. That indicates a more successful elimination of complexes of protein, mineral substances, humoral protection in blood of experimental group of animals.

scholarly journals Application of ultrasonic treatment and synthesized collagen hydrolyzate in the surgical treatment of experimental osteomyelitis

2017 ◽  
Vol 10 (2) ◽  
pp. 137-144
Author(s):  
Natalya Alexandrovna Malkina ◽  
Alexander Alekseevich Andreev ◽  
Anton Petrovich Ostroushko
Keyword(s):  
Bone Tissue ◽  
General Condition ◽  
Ultrasonic Treatment ◽  
Chronic Osteomyelitis ◽  
Mineral Metabolism ◽  
Free Radical Oxidation ◽  
Control Group ◽  
Complex Method ◽  
White Rats ◽  
Experimental Group

Chronic osteomyelitis is one of their complex health problems, since it accounts for up to 6% of cases in the structure of the pathology of the musculoskeletal system, relapses of the disease occur in 30%, and disability is 90% of cases.The aim of the study was to improve the results of treatment of experimental chronic osteomyelitis by applying ultrasound sanation and synthesized collagen hydrolyzate.Materials and methods. The study was carried out on 210 white rats, which were divided into 5 groups: 2 control rats and 3 experimental ones. In the 1st control group, no treatment was performed. In the 2nd control and experimental groups, the hearth was surgically sanitized. In the 1 st experimental group, the focus was performed by ultrasonic treatment. In the 2nd experimental group, the bone cavity was filled with a synthesized hydrolyzate of collagen. In the third experimental group, the combined use of ultrasound sanitation of the focus and filling it with synthesized collagen hydrolyzate was carried out. To assess the dynamics of treatment, the analysis of the general condition of animals, hematological and morphometric studies was performed.Results and their discussions.The dynamics of the general state of animals, the antioxidant defense system, the mineral metabolism, and the evaluation of the histoarchitectonics of bone tissue have been studied. It was noted that against the backdrop of the inflammatory process in chronic osteomyelitis, all the studied indicators indicate a reduced regenerative capacity. Using the developed treatment methods based on ultrasound sanitation, synthesized collagen hydrolyzate, as well as their combination, accelerated relief of inflammatory phenomena, normalization of AOS, mineral metabolism, and regenerative abilities of bone tissue were observed.Conclusions. The developed complex method of treatment based on combined application of ultrasound sanation and synthesized collagen hydrolyzate allowed to shorten the healing period of the wound defect, normalize the general condition of animals, free radical oxidation and mineral metabolism parameters, and accelerate the regenerative capabilities of bone tissue.

scholarly journals ВПЛИВ ІНТРАВАСКУЛЯРНОГО ЛАЗЕРНОГО ОПРОМІНЕННЯ НА ПОКАЗНИКИ КРОВІ У КОРІВ ЗА ЛІКУВАННЯ ГНІЙНО-ЗАПАЛЬНИХ ПРОЦЕСІВ ДИСТАЛЬНОГО ВІДДІЛУ КІНЦІВОК

2014 ◽  
pp. 134-137
Author(s):  
І. І. Юрченко
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Biochemical Parameters ◽  
Inflammatory Process ◽  
Control Group ◽  
Laboratory Results ◽  
Before And After ◽  
Inflammatory Processes ◽  
Hematological And Biochemical Parameters ◽  
Experimental Group

Висвітлені результати лабораторних дослідженькрові (сироватки) за низкою гематологічних і біохі-мічних показників від корів, хворих на гнійно-запальніпроцеси в дистальному відділі кінцівок, до та післявикористання інтраваскулярного лазерного опромі-нення крові. Встановлено, що використання ІЛОК ілокально порошку сульфату міді сприяє нормалізаціїзапального процесу. До того ж у сироватці крові ре-єструється вірогідне зниження активності аспара-гінової трансамінази та збільшення (в контрольнійгрупі) лужної фосфатази. Крім того у дослідній групівідмічали зниження, а в контрольній збільшення від-сотка лімфоцитів, а також збільшення гемоглобіну вдослідній та зменшення його в контролі. Laboratory results of blood (serum) for a range of hematological and biochemical parameters of cows sick on inflammatory processes in the distal extremities before and after use of intravascular laser irradiation of blood have been lit. It has been established that the use of locally ILOK and copper sulfate powder contributes to the normalization of the inflammatory process. In the blood serum is achieved a significant reduction of the activity of aspartic trans a minase and alkaline phosphatase is increased (in the control group).Also in the experimental group we saw a decreasing of the percentage of lymphocytes, and in control one - increasing the percentage of lymphocytes, as well as an increasing of hemoglobin in the experimental group and in control group – its reduction.

Biochemical parameters of blood of calves when using a probiotic based on the strain enterococcus faecium l-3

2020 ◽  
Vol 1 ◽  
pp. 88-92
Author(s):  
M.N. Lebedev ◽  
◽  
S.P. Kovalev ◽  
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Alanine Aminotransferase ◽  
Enterococcus Faecium ◽  
Biochemical Parameters ◽  
Control Group ◽  
Amylase Level ◽  
Serum Amylase Level ◽  
Positive Effect ◽  
Experimental Group

In this paper, we studied the effect of a probiotic, based on the Enterococcus Faeci-um L-3 strain on 20 black-motley calves. The effect of the drug on their biochemical parameters of blood serum were studied. The authors found that this drug, which was fed to calves up to 45 days of age, had a positive effect on the biochemical parameters of blood and on the total animal organism.. So, at 14 days of age, blood serum parame-ters in calves, not receiving the drug, such as: bilirubin, alkaline phosphatase, alanine aminotransferase and aspartate aminotrans-ferase levels were significantly higher than in calves that received probiotic, which indi-cates possible dystrophic changes in the liv-er. Also, at 14 days of age in cows, reciev-ing the drug , the blood serum amylase level was significantly higher than of the calves, not receiving the drug. By the age of 30 days, the tendency to a higher content of bilirubin, alkaline phosphatase, alanine ami-notransferase and aspartate aminotransferase in the blood serum, persisted in the calves of the control group, compared with the ani-mals of the experimental group. By the age of 45 days, the levels of bilirubin, alanine aminotransferase and alkaline phosphatase were also significantly higher in control group of animals, than in experimental group and were 5.0 ± 0.56 μmol / L and 3.72 ± 0.4 μmol / L , 30.66 ± 2.9 IU / L and 16.52 ± 1.2 IU / L, 345.7 ± 30.7 and 226.6 ± 24.1, respectively.The results obtained, indicated that regular feeding of a probiotic based on Enterococcus Faecium L-3 to calves of the milky period, contributes to the normaliza-tion of biochemical parameters and greater resistance to gastrointestinal disorders.

INFLUENCE OF STOCK HOUSE LIGHT ON DAIRY PRODUCTIVITY OF COWS

2020 ◽  
Keyword(s):  
Blood Serum ◽  
Physiological State ◽  
Light Level ◽  
Inorganic Phosphorus ◽  
Control Group ◽  
Hemoglobin Content ◽  
Milk Productivity ◽  
Reproductive Ability ◽  
The Cost ◽  
Experimental Group

The article is devoted to the solution of an urgent problem- influence of different lighting modes on the dairy productivity of cows. 2 groups of cows with 20 heads each were formed. In control group, light in the cowshed was 50-75 Lux for a light period of 7.5 h in January to 16.5 h in June, and in experimental group - 150-200 Lux and 16 h, respectively. It was found that the intensity and duration of illumination affects physiological state, reproductive ability and milk productivity of cows. In the experimental group of cows, compared with the control group, hemoglobin content in blood increased by 4.6% (P < 0.01), red blood cells - by 20.6% (P < 0.05), total protein - by 11.2% (P < 0.001), glucose - by 39.1% (P < 0.05). There was a tendency to increase the total calcium and inorganic phosphorus in blood serum of cows of the experimental group. The level of alkaline phosphatase in blood serum of cows in the control group was 71.5% (P < 0.01) higher than that of cows in the experimental group. Milk yield per 1 cow in the experimental cowshed was 433 kg more than in the control. The cost of 1 kg of milk in the experimental group was 0.94 rubles lower, and the profitability of milk production and sales is 9.42% higher than in the control group. To increase the milk productivity of cows, it is recommended to increase light level in barns for tethered keeping to 150-200 Lux, with the duration of lighting in the winter and transition periods of year up to 16 hours per day.

Reproductive traits and blood biochemical parameters of sows when using a vitamin feed additive “LipoCar”

2020 ◽  
pp. 25-31
Author(s):  
I. A. Pushkaryev ◽  
N. M. Kostomakhin ◽  
S. V. Burtseva ◽  
N. A. Novikov
Keyword(s):  
Blood Serum ◽  
Biochemical Parameters ◽  
Total Duration ◽  
Reproductive Traits ◽  
Feed Additive ◽  
Control Group ◽  
Large White ◽  
Basic Diet ◽  
Blood Biochemical ◽  
Experimental Group

The reproductive traits of sows are of paramount importance in the production of high-quality pork. The world experience of pig farming shows the need to solve first of all the feed problem. Diets unbalanced in the content of vitamins restrain the manifestation of genetically inherent high prolificacy of sows. The purpose of the research was to study the influence of feeding the vitamin feed additive “LipoCar” on the reproductive traits of sows and the biochemical parameters of blood serum. The results of research on the introduction of vitamin feed additive “LipoCar” into the diet of pregnant sows have been presented in the article. The experiment has been carried out in the production conditions in JSC “Linevsky pedigree farm” on sows of Large White breed. Pregnant sows have received a basic diet, balanced for all the normalized elements of nutrition. Sows of the experimental group in the composition of the basic diet fed the feed additive “LipoCar”. The dosage of the drug “Lipocar” was 2,1 g/head/day. “Lipocar” has been fed for 20 days. The total duration of the experiment was 3 months. When introducing the vitamin feed additive “LipoCar” into the main diet of sows in the second half of pregnancy the increase in the number of piglets in the litter by 1,6-6,8 % and the weight of the litter – by 5,6-56,2 % (P ≤ 0,001) have been revealed in comparison with the control group of animals. There was a higher content of carotene in the blood serum of sows of the experimental group by 50,0 % (P ≤ 0,01), vitamin A - by 48,7 % (P ≤ 0,05), and total protein – by 4,9 % (P ≤ 0,05).

scholarly journals The cell protection of weated pigs for probiotics

2020 ◽  
pp. 111-119
Author(s):  
L. Bondarenko
Keyword(s):  
Immune System ◽  
Blood Serum ◽  
Phagocytic Activity ◽  
Lysozyme Activity ◽  
The State ◽  
Natural Resistance ◽  
Phagocytic Index ◽  
Control Group ◽  
Stressful Situation ◽  
Experimental Group

The immune system is central to ensuring the consistency of the body's homeostasis. The state of the body's natural resistance is determined by a set of non-specific protective mechanisms. Lymphocytes and phagocytes are actively involved in maintaining immunity. Lymphocytes recognize the antigens of pathogenic microorganisms, and phagocytes absorb and destroy the pathogens themselves. During the weaning of piglets from sows there is a decrease in the protective forces of their body. During this period, the natural resistance of the piglets is reduced due to the stressful situation caused by changing conditions of confinement, the transition to full feed and lack of sows. The immune system of weaning pigs is relatively weak, so when exposed to environmental and technological stressors, they become susceptible to various diseases. The use of probiotic drugs stimulates the activity of the immune system, prevents stress and immunodeficiency. One of these probiotics is the probiotic of domestic production Protecto-active. It w observed the the influence of the probiotic Protecto-active on the indices of nonspecific resistance of the young pigs organism to the growth. An increase in bactericidal activity of blood serum by 12.10% (P <0.05) and lysozyme activity of blood in the piglets of the experimental group was increased by 3.71% compared to control, which indicates the activation of the body's defenses and the increase in adaptive capacity. An important step in the study of the influence of the probiotic Protekto-active on the state of the immune system is to determine the phagocytic activity of neutrophils, phagocytic index and phagocytic number. In the experimental group of piglets that were fed the probiotic Protecto-active, we found an increase in leukocyte phagocytic activity by 9.0% (P <0.001), a phagocytic index by 51.7% (P<0.001) and a phagocytic number by 24.8% ( P <0.01) compared with the control group. Thus, using a probiotic Protecto-active, all indicators of phagocytosis increase: the number of phagocytes increases, their ability to capture microorganisms and increases their digestive capacity, it increases the bacterial and lysozyme activity of blood serum, which is positively reflected in the immunobiosity. Key words: probiotic, phagocytosis, phagocytic index, phagocytic number, phagocytic activity of leukocytes, cellular immunity, piglets

scholarly journals Healing of abdominal wall aponeurosis of rats after incision with either cold scalpel or electrocautery

2007 ◽  
Vol 22 (suppl 1) ◽  
pp. 12-15 ◽  
Author(s):  
Ademar Garcia ◽  
José Eduardo Aguilar-Nascimento ◽  
Eduardo Mulinari Darold ◽  
Rodolfo Edson de Franco Pimentel ◽  
Eloísa Almeida Curvo ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Histological Study ◽  
Control Group ◽  
Hydroxyproline Content ◽  
Tissue Samples ◽  
Significant Difference ◽  
Experimental Group

PURPOSE: To compare the healing of abdominal wall aponeurosis of rats after incision with either cold scalpel or electrocautery. METHODS: Twelve male adults Wistar inbreed rats weightin 258-362g entered the experiment. They were randomly divided in two groups, control group (N= 6) and experimental group (N= 6). All animals underwent anesthesia followed abdominal wall incision with either cold scalpel (controls) or electrocautery (experimental group). The animals were killed on the 10th day and necropsied. Tissue samples containig the abdominal wall aponeurosis were sent for both histological study and hydroxyproline content assay. RESULTS: The histological study showed no significant difference between the two groups. Hydroxiproline content in experimenral group (128,56 [78,98-198,92] µg /100m) was not significantly different from control group (140,27 [116,20-166,59] mg /100ml; p = 0.53). CONCLUSION: Healing of abdominal wall after dieresis with either cold scalpel or electrocautery are equivalent and do not differ.

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