scholarly journals The cell protection of weated pigs for probiotics

2020 ◽  
pp. 111-119
Author(s):  
L. Bondarenko
Keyword(s):  
Immune System ◽  
Blood Serum ◽  
Phagocytic Activity ◽  
Lysozyme Activity ◽  
The State ◽  
Natural Resistance ◽  
Phagocytic Index ◽  
Control Group ◽  
Stressful Situation ◽  
Experimental Group

The immune system is central to ensuring the consistency of the body's homeostasis. The state of the body's natural resistance is determined by a set of non-specific protective mechanisms. Lymphocytes and phagocytes are actively involved in maintaining immunity. Lymphocytes recognize the antigens of pathogenic microorganisms, and phagocytes absorb and destroy the pathogens themselves. During the weaning of piglets from sows there is a decrease in the protective forces of their body. During this period, the natural resistance of the piglets is reduced due to the stressful situation caused by changing conditions of confinement, the transition to full feed and lack of sows. The immune system of weaning pigs is relatively weak, so when exposed to environmental and technological stressors, they become susceptible to various diseases. The use of probiotic drugs stimulates the activity of the immune system, prevents stress and immunodeficiency. One of these probiotics is the probiotic of domestic production Protecto-active. It w observed the the influence of the probiotic Protecto-active on the indices of nonspecific resistance of the young pigs organism to the growth. An increase in bactericidal activity of blood serum by 12.10% (P <0.05) and lysozyme activity of blood in the piglets of the experimental group was increased by 3.71% compared to control, which indicates the activation of the body's defenses and the increase in adaptive capacity. An important step in the study of the influence of the probiotic Protekto-active on the state of the immune system is to determine the phagocytic activity of neutrophils, phagocytic index and phagocytic number. In the experimental group of piglets that were fed the probiotic Protecto-active, we found an increase in leukocyte phagocytic activity by 9.0% (P <0.001), a phagocytic index by 51.7% (P<0.001) and a phagocytic number by 24.8% ( P <0.01) compared with the control group. Thus, using a probiotic Protecto-active, all indicators of phagocytosis increase: the number of phagocytes increases, their ability to capture microorganisms and increases their digestive capacity, it increases the bacterial and lysozyme activity of blood serum, which is positively reflected in the immunobiosity. Key words: probiotic, phagocytosis, phagocytic index, phagocytic number, phagocytic activity of leukocytes, cellular immunity, piglets

scholarly journals THE EFFECT OF «ARCUSIT» ON BIOCHEMICAL AND IMMUNOLOGICAL PARAMETERS BLOOD SERA IN SILVER-BLACK FOX (SILVER-BLACK FOX)

2021 ◽  
Vol 247 (3) ◽  
pp. 192-197
Author(s):  
I.I. Okulova ◽  
◽  
I.A. Domsky ◽  
Yu.A. Berezina ◽  
Z.N. Beltyukova ◽  
...  
Keyword(s):  
Experimental Data ◽  
Blood Serum ◽  
Bactericidal Activity ◽  
Lysozyme Activity ◽  
The Body ◽  
Phagocytic Index ◽  
Control Group ◽  
Immunological Parameters ◽  
Nonspecific Resistance ◽  
Experimental Group

The use of «Arkusit» at a dose of 20 μg / kg of body weight indicates a beneficial effect on the factors of nonspecific resistance of the silver-black fox. The concentration of alkaline phosphatase in the blood serum in the experimental group decreased by 1.8 times (P <0.001), the ALT index - by 1.2 (P <0.05). The concentration of glucose in the experimental group increased 2 times (P <0.05) compared with the control group. In animals of the experimental group, the lysozyme activity of blood serum increased 1.3 times (P <0.05), the OPR reaction and the phagocytic index - 1.4 times (P <0.05), the bactericidal activity of blood serum - 1.8 times (P <0.05). «Arcusit» stimulates the synthesis of gamma globulins, increases the opsonophagocytic, bactericidal activity of blood serum. Thus, the analysis of experimental data indicates that the drug «Arcusit» has a pronounced immunostimulating effect, providing the activation of factors of nonspecific resistance of the body of the silver-black fox.

scholarly journals NATURAL RESISTANCE AND DEVELOPMENT OF EGG-TYPE REPAIR YOUNG CHICKS

2021 ◽  
Vol 246 (2) ◽  
pp. 132-136
Author(s):  
А.А. Menkova ◽  
◽  
V.N. Shilov ◽  
Е.М. Tsygankov ◽  
◽  
...  
Keyword(s):  
Blood Serum ◽  
Phagocytic Activity ◽  
Lysozyme Activity ◽  
Natural Resistance ◽  
Poultry Farm ◽  
Immune Protection ◽  
Poultry House ◽  
Blood Neutrophils ◽  
And Control ◽  
Experimental Group

Blood and lymph make up the circulatory link of the bird's immune system. It is necessary to note the humoral factor of immune protection-lysozyme. The purpose of scientific research is to study the level of natural resistance and development of egg-type chickens after aerosol application of the disinfectant Argodez. Research and production experiments were conducted in the conditions of the poultry farm "snow", Bryansk region, p. Voucher. Aerosol disinfection was performed in the poultry house before settling in with day-old chickens. Test shop were treated once Arcadetm of 0.01%, the rate of 2 ml/m3, and control Desalina - f - 5 ml/m3., with the generator cold fog IGEBA -UNIPRO-5. At the age of 24 hours, two groups of egg - type chickens, the Lohmann-brown cross, were formed using the method of analogous groups. Blood was taken from the underwing vein and by decapitation, before feeding, at the daily, 30-day, 60-day age. Bactericidal, lysozyme, and phagocytic activity of blood serum was determined according to generally accepted methods. Control weighing of chickens of both groups was performed on days 30 and 60 of the study. A significant increase in phagocytic activity of blood neutrophils was found in the experimental group of chickens on the 30th-21.40 % and 60th day of research-25.40 %, as well as a tendency to increase the bactericidal activity of blood serum by 1.90 % and 7.90 % and the lysozyme activity of blood serum by 8.46 % and 9.17 %. It was also found to increase safety by age periods. The highest safety was observed at the age of 1-30 days and was 99.50 %, then 30-60 – 99.10 %.

scholarly journals Intestinal histostructure and immune protection activity of age -1+ carp after consumption of a prebiotic feed additive

2021 ◽  
Vol 4 (2) ◽  
pp. 31-37
Author(s):  
O. P. Dobrianska ◽  
M. I. Zhyla ◽  
O. I. Vishchur ◽  
O. V. Deren ◽  
M. Z. Koryliak
Keyword(s):  
The Body ◽  
Natural Resistance ◽  
Feed Additive ◽  
Phagocytic Index ◽  
Control Group ◽  
Average Weight ◽  
Histological Structure ◽  
Nonspecific Resistance ◽  
Positive Effects ◽  
Experimental Group

Currently, it is important in aquaculture to study and use products and additives, which have positive effects on the digestibility of nutrients by helping to normalize the intestinal microflora, thereby modulating fish immune response. The aim of the study was to assess the effect of a prebiotic based on mannan oligosaccharides produced from the outer walls of Saccharomyces cerevisiae cells on the histological structure, morphometric state of the intestine and activity of cellular and humoral components of nonspecific resistance of age -2+ carp. The experiment was conducted in four analogous ponds, which were stocked with age-1 scaly carp with an average weight of 55–58 g with a stocking density of 1000 fish/ha. Carp had been fed balanced compound feed for 60 days. Experimental groups of fish were fed with a prebiotic as a feed additive in the amount of: 0.025 % – Experiment 1, 0.05 % – Experiment 2 and 0.075 % – Experiment 3. Use of the studied prebiotic did not damage the intestinal histological structure of carp of the experimental groups. The villi height and intestinal crypt depth of carp in Experiment 1 (P < 0.05) and Experiment 2 (P < 0.01) were larger compared to the control group. Experiment 3 showed destructive changes in intestinal mucosa. The results of histological examination indicated the effectiveness of the use of the studied prebiotic in the amount of 0.025 and 0.05 % in the diet of age -1+ carp due to the absence of pathological changes and active morphofunctional state of the intestine, which, in turn, had a positive effect on feed absorption and metabolic processes in fish body. Analysis of the indicators of cellular and humoral links of natural resistance showed that in carp of the second and third experimental groups lysozyme activity of serum was 5 and 11 % (P < 0.001) higher than in the control group. Similar changes, though found in a greater extent, were recorded in the study of bactericidal activity of blood serum, especially in individuals of the second and third experimental groups (by 6.8 (P < 0.01) and 15.2 %). While with carps of the third experimental group, which used a prebiotic drug in the amount of 0.075 %, the content of CEC was 20.5 % higher than in the control group, which indicates an additional antigenic load on the body and is an unfavorable diagnostic factor. At the same time, with the fish of the second experimental group, which received, respectively, 0.05 % of the supplement of the drug “Actigen” to the main diet, probably higher phagocytic activity of blood neutrophils was recorded. A direct dependence of the growth of the phagocytic index and the phagocytic number of neutrophils on the dose of the studied prebiotic was detected. Immunological studies indicated a dose-dependent effect of the prebiotic on the activity of cellular and humoral components of non-specific resistance of carp. Given this, for the effective functioning of innate protective mechanisms, for the increase of the body's immune potential and productivity, it is most appropriate to apply to the diet of carp prebiotic supplement “Actigen” in the amount of 0.025 and 0.05 %.

scholarly journals Interconnection of the immune system and the intensity of the oxidative processes under conditions of prolonged exposure to small doses of radiation

2018 ◽  
Vol 9 (2) ◽  
pp. 167-176 ◽  
Author(s):  
V. L. Sokolenko ◽  
S. V. Sokolenko ◽  
V. I. Sheiko ◽  
O. V. Kovalenko
Keyword(s):  
Oxidative Stress ◽  
Immune System ◽  
Phagocytic Activity ◽  
Prolonged Exposure ◽  
Stress Index ◽  
Control Group ◽  
Oxidative Stress Index ◽  
Small Doses ◽  
Oxidative Processes ◽  
Experimental Group

This research studied the interrelationship between the immune and oxidative-antioxidant systems in a group of individuals who had lived for a long time in areas contaminated with radionuclides after the Chernobyl catastrophe and as a result experienced prolonged exposure to small doses of ionizing radiation. We have examined a group of 100 students aged 18–24, where 50 of them formed the control group and the remaining 50 belonged to the experimental group as they arrived from the territories of enhanced radioecological control (IV radiation zone, density of soil contamination by isotope 137Cs 3.7 x 104 – 18.5 x 104 Bq/m2). Here we determined the level of cortisol, leukocytes and their populations, the levels of lymphocyte subpopulations with phenotypes CD3+, CD5+, CD4+, CD8+, CD16+, CD72+, immunoregulatory index CD4+/CD8+, indicators of phagocytic activity of neutrophils and monocytes, IgG (H), IgM (H), IgA (H), malondialdehyde (MDA), ceruloplasmin (CP), transferrin (Tr), sulfhydryl (SH); and also calculated the oxidative stress index (OSI). We performed the analysis twice: in the absence/presence of additional emotional stress such as an examination session. The studies showed an increase in the oxidative stress index in the group examined from the experimental cluster, especially in terms of emotional stress. At the same time, the neutrophil level increased, but phagocytic activity of neutrophils and monocytes, the relative and absolute number of lymphocytes with phenotypes CD3+, CD5+, CD4+, CD4+/CD8+, and IgG levels decreased. Consequently we revealed the negative correlation between the indexes of oxidative stress in the group of examined (the oxidative stress index (ISO)/the level of malonic dialdehyde (MDA)) and the parameters of phagocytic activity of monocytes, the immunoregulatory index CD4+/CD8+, and the number of lymphocytes with the CD16+ phenotype. In this study we demonstrated the decrease in the participation of ceruloplasmin (CP) as an important antioxidant factor in maintaining the immune homeostasis of the group examined from radiation-contaminated areas compared with control group. The evidence of this is the lack of reliable correlation between ceruloplasmin level and immune system parameters. Moreover we found that radiation-induced intensification of oxidative processes in the experimental group grew in conditions of additional stresses of an emotional nature. Besides, it was accompanied by a significant correlation in the level of oxidative stress and phagocytic activity parameters. Reducing phagocytic activity and the CD4+/CD8+ index on the background of oxidative stress increase can be considered as a sign of immune system ageing, while a decrease in the number of lymphocytes with the CD16+ phenotype is a sign of antitumor defense inhibition. Thus, we draw the conclusion that the inhabitants of the territories of strengthened radioecological control, undergoing exposure to small doses of ionizing radiation from birth, show a significant imbalance of redox homeostasis, which creates the preconditions for immunoreactivity pathology development at the level of both innate and acquired immunity.

scholarly journals Stress state and immunological status of young pigs

2019 ◽  
pp. 95-98
Author(s):  
M. V. Chornyi
Keyword(s):  
Stress State ◽  
Blood Serum ◽  
Bactericidal Activity ◽  
Total Protein ◽  
Live Weight ◽  
Natural Resistance ◽  
Control Group ◽  
Immunological Status ◽  
Young Pigs ◽  
Experimental Group

The paper presents the results of studies on the effect of regrouping of piglets at different ages on the resistance of their organisms, immunological status, productive qualities and safety of young pigs. The work was performed at “Stas” LLC on large white breed pigs and their crossbreeds. For the experiment, three groups of piglets were formed from suckling sows — analogues. In the experiment 60 animals from birth up to two months of age were used, 20 animals in each experimental group. The control group of piglets was raised from birth up to 60 days of age in nests, and then they were moved to the rearing workshop; Experimental 1 group was kept up to 10 days old in nests, and then a one-time rearrangement was performed according to live weight. Piglets from experimental group 2 were regrouped according to live weight at 5, 10, 15, 21, 30 days of age. To assess the natural resistance of piglets due to the above groups, hematological, biochemical (total protein, protein fractions) methods, immunological (immunoglobulins of classes IgA, IgM, IgG), and natural resistance (bactericidal activity of blood serum) were used, serum lysozyme activity (LASK), phagocytic neutrophil activity (FAN), phagocytic index (FI), ethological, zootechnical, mathematical. To assess the natural resistance of piglets the following research methods were used: generally accepted zoo veterinary methods (live weight, morbidity, safety), hygienic (microclimate conditions, sanitary regime), hematological (morphological blood composition), biochemical (total protein, protein fractions), immunological (immunoglobulins of classes IgA, IgM, IgG), natural resistance (bactericidal activity of blood serum), ethological and mathematical methods. Results of work. It was found that when growing piglets, nesting, without moving, caused their growth and development without any noticeable physiological disturbances, both from the blood and ethology. In animals (Experimental-1 groups) which underwent a single regrouping in a 10 day reward, their lag in live weight by 12.65% was recorded, compared with the control, with two and three-time movement (Experimental-2) — by 16.10%. Their SSPs were 28% less and 14.7% lower than in the control piglets. Patients with symptoms of diarrhea were identified in the control: in 3–4 daily reimbursement — 1%, in Experimental-1 — 5%, Experimental-2 — 10–15%. The resistance of young animals to gastrointestinal diseases according to the Melenberg coefficient in the Experimental-2 group was 6.13–7.48, in control group it was 0.34, and the safety did not exceed 80.2%. According to the level of immune status, animals from Experimental-2 group were inferior: by BASK — by 25.15% (28 days of age), by LASK — by 7.56%, by FAN — by 10.7% compared with peers from Experimental-1 groups. By the number of eosinophils (Experimental-2 group), characterizing the stress state of piglets, their decrease was observed within 10–12 days, not more than 5 days — in animals from Experimental-1 group and up to 3 days — from control

scholarly journals Nonspecific reactivity of the rabbits organism when exposed to cysticercosis

2019 ◽  
Vol 21 (94) ◽  
pp. 132-135
Author(s):  
Y. V. Duda
Keyword(s):  
Blood Serum ◽  
Bactericidal Activity ◽  
Immune Complexes ◽  
Research Group ◽  
Phagocytic Activity ◽  
Lysozyme Activity ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
E Coli ◽  
Test Culture

One of the actual rabbits breeding problem is the reduction of their resistance, which is caused by the spread of individual invasive diseases, especially cysticercosis, which is caused by the larval Cysticercus pisiformis stage of the mature cestode Taenia pisiformis. The study was conducted on 38 male rabbits age 3–4 months, weighing 3.5–4.0 kg of the Californian breed, selected by analogy. Animal were separated into two groups: healthy animals (control group) and sick animals (research group). After blood collection, slaughter was conducted and the presence of cysticercus cysts was visually determined in the organisms of the rabbits. Control animals received a balanced standard granular feed and water without limit; research group in addition to the standard granulated feed with water received flaccid hay. The animals were kept in mesh single-tier cages in the room, according to the current veterinary and sanitary standards. The definition of phagocytic activity of neutrophils was carried out with the addition of standardized to 2500000000/ml suspension of daily culture of E. coli 055K59№3912/41. The bactericidal activity of blood serum was determined by the method Smirnova A.V. and Kuzmina T.A. in relation to the E. coli microbial test-culture 055K59№3912/41. The serum lysozyme activity was determined by the Nephelometric method using the Dorofachuk V.G. method to the microbial test culture Micrococcus luteus ATSS9341. Circulating immune complexes were determined using polyethylene glycol in borate buffer (pH 8.4). It was found that in blood of sick animals phagocytic activity is lower than in blood of healthy ones (respectively, 41.82 ± 1.51% versus 47.38 ± 1.10%, P < 0.01). The main cells involved in phagocytosis are leukocytes, in particular neutrophils and eosinophils. A low indicator of phagocytic activity shows depressed phagocytosis in the organism of animals suffering from cysticercosis of rabbits. The phagocytic number in the blood of rabbits of the experimental group was significantly lower by 0.51 units (P < 0.05) as compared to the control group and correlated with the index of phagocytic activity. An important element of immunity are indicators of bactericidal and lysozyme activity of blood serum. Low bactericidal activity of serum by 12.88% (P < 0.01) and lysozyme activity in rabbits with Cysticercus pisiformis also indicates a weakening of the factors of nonspecific natural resistance of the organism. Analyzing the level of circulating immune complexes, we found a high level of medium (9.14 ± 0.40 vs 5.58 ± 0.50) and small (9.21 ± 1.35 vs 4.64 ± 0.68) CIC for cysticercosis, respectively, 1.64 (P < 0.01) and 1.98 times (P < 0.001) against the control. This indicates the inhibition of the immunobiological activity in the organism of rabbits as a result of the combination of specific antibodies with the products of the exchange of helminths.

scholarly journals The state of the immune system of dogs in experimental toxocariasis

2020 ◽  
Vol 3 (3) ◽  
pp. 20-24
Author(s):  
W. S. Said ◽  
V. V. Stybel ◽  
B. V. Gutyj ◽  
O. B. Pryima ◽  
A. G. Sobolta ◽  
...  
Keyword(s):  
Immune System ◽  
Cellular Immunity ◽  
Phagocytic Activity ◽  
Physiological State ◽  
Toxocara Canis ◽  
The State ◽  
Phagocytic Index ◽  
Humoral Immune ◽  
National University ◽  
High Level

The aim of the study was to determine the state of the dogs' immune system in experimental toxocariasis. The work was performed during 2017-2020 at the Department of Parasitology and Ichthyopathology in Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv. The 12 dogs aged 2 to 4 months were used for research studies. Two groups were formed of six animals in each: control and experimental. Puppies of the experimental group (E) were infected with the causative agent of toxocariasis at a dose of 5000 invasive eggs of Toxocara canis per kg of body weight. The control puppies were clinically healthy. At the clinical display of toxocariasis invasion at dogs the cellular, humoral and nonspecific links of the immune system are suppressed and the secondary immunodeficiency comes. Suppression of cellular immunity was accompanied by a decrease in the T- and B-lymphocytes count in the blood of dogs of the (E) group, which indicates the suppression of the lymphoid immune system and a reduction in the resistance of animals. Along with the fall in cellular immunity in infected puppies, suppression of the nonspecific immune system was established, which is manifested by a decline in the phagocytic activity of neutrophils and a decrease in the phagocytic index. Thus, on the 15th and 20th days of the test, the phagocytic activity of neutrophils reduced to 1.6 and 3.9 %, while the phagocytic index – to 5.4 and 6.9 %, respectively. In the study of antimicrobial activity of serum of infected dogs, T. canis found inhibition of bactericidal and lysozyme activity, which reflects the suppression of the physiological state of the humoral immune system of animals with the development of toxocariasis. On the 25th day of the research, a deccline in BABS to 24.0 ± 2.7 %, while in the (C) group of dogs this figure was 30.6 ± 1.9 %. The lowest LABS was on the 25th day of the (E) in animals that were experimentally infected with the pathogen toxocariasis, where compared with the (C) group, this figure decreased by 6.0 %, respectively. The high level of CIC in the serum of dogs infected with T. canis indicates the suppression of the immune system of their body due to the accession of specific antibodies to the products of metabolism of toxocara, which act as antigens.

scholarly journals Phagocytosis in Staphylococcus aureus bacteriaemia in dogs

2007 ◽  
Vol 61 (1-2) ◽  
pp. 43-52 ◽  
Author(s):  
Tsvetan Chaprazov ◽  
Ivan Borissov ◽  
Evgeni Slavov ◽  
Petco Dzhelebov
Keyword(s):  
Reactive Oxygen Species ◽  
Staphylococcus Aureus ◽  
Innate Immunity ◽  
Phagocytic Activity ◽  
Reactive Oxygen ◽  
Phagocytic Index ◽  
Control Group ◽  
Oxygen Species ◽  
Species Production ◽  
Experimental Group

Staphylococcus aureus is the leading pathogenic cause of nosocomial infections, especially in bacteriaemia and sepsis. Phagocytosis is known to be an essential factor of innate immunity. The aim of this study was to evaluate phagocytic activity of leucocytes in experimental bacteriaemia. Bacteriaemia was induced in six adult male mongrel dogs (experimental group) by intravenous injection of Staphylococcus aureus isolate (1.2x109 cells/ml). Phagocytic activity was evaluated before infection (0 h.), and on the 2nd, 6th, 24th, 48th hour, and on the 7th, 14th and 21st day after infection. Six control animals were tested in the same dynamics. Phagocytic activity was evaluated by using the Nitrotetrazolium blue reduction (NBT) test, and the immune fluorescence method (Samnaliev et al., 1995) for detection of phagocytic index and percentage of phagocyting leucocytes (FITC marked Staphylococcus aureus were used). Percentage of phagocyting leucocytes showed an increase in experimental animals, compared to control animals, on the 24th h. Production of reactive oxygen species, evaluated by NBT, showed changes within experimental group as follows: an increase on the 24th h. compared to the 2nd h., 6th h., and the 14th day after infection; and an increase on 48th h., compared to the 2nd h. and 14th day. Comparison of reactive oxygen species production between groups, revealed an increase in the experimental group, as compared to the control group, on the 24th h. and on the 48th h. In conclusion cell elements of innate immunity are mostly activated on the first two days after inducing Staphylococcus aureus bacteriaemia in dogs.

scholarly journals Use of probiotic lactobacilli to increase non-specific resistance and productivity of rabbits

2020 ◽  
Author(s):  
A.N. Ovcharova ◽  
Keyword(s):  
Growth Rate ◽  
Bactericidal Activity ◽  
Phagocytic Activity ◽  
Specific Resistance ◽  
Live Weight ◽  
Phagocytic Index ◽  
Control Group ◽  
Control Parameters ◽  
Probiotic Lactobacilli ◽  
Experimental Group

The article presents the results of the effectiveness of probiotic lactobacilli on the productivity and non-specific resistance of rabbits. The phagocytic activity of neutrophils increased by 10 %, which turns out to be statistically significant. The bactericidal activity of blood increased by 8 % in comparison with the control group; the phagocytic index was also higher in the experimental group by 2.02. The content of lysozyme in the blood serum of rabbits in the experimental group was significantly higher than the control parameters by 18.6 micrograms/ml. As tentative results, we report an increase in the live weight and growth rate in the experimental group of rabbits.

scholarly journals Influence of vitamins E and C on the indices of pseudoeozinofiles fagocytosis in chickens broilers blood of cross ROSS-308

2018 ◽  
Vol 20 (92) ◽  
pp. 169-171
Author(s):  
L. V. Romanovych ◽  
B. M. Kurtyak ◽  
M. S. Romanovych ◽  
O. I. Vishchur ◽  
D. I. Mudrak
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Phagocytic Activity ◽  
Broiler Chickens ◽  
Coli Strain ◽  
Phagocytic Index ◽  
Control Group ◽  
Free Access ◽  
Vitamins E And C ◽  
Experimental Group

The purpose of the research was to clarify the effect of chicken broilers feeding in the composition of mixed fodder with Vitamins E and C on the cellular link of non-specific resistance of the organism and, in particular, the parameters of pseudoiesinophils phagocytosis in blood. The research was carried out on chickens broiler – 308 grown in the farm “Fedyuk M” in the Zolochiv district of Lviv region. The keeping of the chickens was cellular with free access to feed and water. Experiments were conducted in 4 groups of broiler chickens of 100 chicks in each according to the scheme: the control group was fed with the standard feed (SC) according to the existing norms recommended for the cross ROSS-308; the first experimental group in addition to the SC received vitamin Е 1g/10 kg of mixed fodder; the second experimental group received vitamin C 2.5 g/10 kg of mixed fodder; the third experimental groups in addition to the ration received Vitamin C 2.5 g/10 kg and Vitamin E 1 g/10 of mixed fodder. For carrying out of immunological researches in chickens blood was taken in different age periods: 27-, 34- and 41-day-old age. In the whole blood, the phagocytic activity of pseudoiesinophils (FA), phagocytic index (FI) and phagocytic number (FF) were determined. For research, the daily culture of Escherichia coli (strain VKM-125) was used. The stimulatory effect of vitamins E and C in the composition of mixed fodder for chicken broiler broilers on the indices of pseudoiesinophils phagocytosis of blood was determined. As evidenced by a significantly higher phagocytic activity, phagocytic index and phagocytic count of pseudoiselinophils in blood of chickens in the experimental groups by comparison with the control group. At the same time there was a direct correlation between phagocytic activity and phagocytic index and index in blood of chickens broiler in experimental groups. At the same time, the ability of blood pseudoiesinophils to phagocytosis of microbial cells was higher in chicken broilers, which were fed with vitamin E and C.

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