Application of ultrasonic treatment and synthesized collagen hydrolyzate in the surgical treatment of experimental osteomyelitis

Chronic osteomyelitis is one of their complex health problems, since it accounts for up to 6% of cases in the structure of the pathology of the musculoskeletal system, relapses of the disease occur in 30%, and disability is 90% of cases.The aim of the study was to improve the results of treatment of experimental chronic osteomyelitis by applying ultrasound sanation and synthesized collagen hydrolyzate.Materials and methods. The study was carried out on 210 white rats, which were divided into 5 groups: 2 control rats and 3 experimental ones. In the 1st control group, no treatment was performed. In the 2nd control and experimental groups, the hearth was surgically sanitized. In the 1 st experimental group, the focus was performed by ultrasonic treatment. In the 2nd experimental group, the bone cavity was filled with a synthesized hydrolyzate of collagen. In the third experimental group, the combined use of ultrasound sanitation of the focus and filling it with synthesized collagen hydrolyzate was carried out. To assess the dynamics of treatment, the analysis of the general condition of animals, hematological and morphometric studies was performed.Results and their discussions.The dynamics of the general state of animals, the antioxidant defense system, the mineral metabolism, and the evaluation of the histoarchitectonics of bone tissue have been studied. It was noted that against the backdrop of the inflammatory process in chronic osteomyelitis, all the studied indicators indicate a reduced regenerative capacity. Using the developed treatment methods based on ultrasound sanitation, synthesized collagen hydrolyzate, as well as their combination, accelerated relief of inflammatory phenomena, normalization of AOS, mineral metabolism, and regenerative abilities of bone tissue were observed.Conclusions. The developed complex method of treatment based on combined application of ultrasound sanation and synthesized collagen hydrolyzate allowed to shorten the healing period of the wound defect, normalize the general condition of animals, free radical oxidation and mineral metabolism parameters, and accelerate the regenerative capabilities of bone tissue.

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The impact of humic acid additives on mineral metabolism of rabbits in the postnatal period of ontogenesis

Regulatory Mechanisms in Biosystems ◽

10.15421/022044 ◽

2020 ◽

Vol 11 (2) ◽

pp. 289-293

Author(s):

M. A. Rybalka ◽

L. M. Stepchenko ◽

O. O. Shuleshko ◽

L. V. Zhorina

Keyword(s):

Alkaline Phosphatase ◽

Humic Acid ◽

Blood Serum ◽

Bone Tissue ◽

Mineral Metabolism ◽

Histological Study ◽

Postnatal Period ◽

Control Group ◽

Copper And Zinc ◽

Experimental Group

Humic acids are characterized by chelating properties due to which they are able to influence the mineral metabolism in animals. Rabbits have species-specific characteristics of mineral metabolism, which distinguishes them from most species of mammals. For the experiment, 16 rabbits of 45-day-old of Hyplus breed were divided into the experimental and control group of 8 rabbits in each. The animals were 15 days in the preparatory period. The rabbits of the experimental group received humic acid additive individually with water in the amount of 5 mg/kg, the rabbits of the control group received pure water in the same amount. The ability of humic acid additive to increase the content of calcium, ionized calcium, iron, to correct the content of copper and zinc, and also to increase the activity of alkaline phosphatase in the blood serum of rabbits of the experimental group has been proved. We observed the early effect of the humic acid additive on increasing the content of iron and calcium ionized and a later effect on the activity of alkaline phosphatase and an increase in the content of copper in the blood serum. The influence of humic acid additive on the distribution of calcium, phosphorus, manganese, copper and zinc in bone tissue is determined. According to a histological study, there was a significant increase in the number of layers of osteons and osteoblasts in the bone tissue of the femur, an increase in the number of osteons and osteoblasts in the bone tissue of the sternum and an increase in the number of columns of chondrocytes and an increase in the number of chondrocytes in the column in the cartilaginous tissue of the sternum. The results of a histological study of bone tissue, together with an increase in structural macroelements in it and redistribution of osteotropic trace elements in the blood and bone tissue suggest the possibility of using a humic acid additive to intensify the growth and mineralization of bone tissue, which can improve the state of bone tissue of rabbits in the postnatal period ontogenesis.

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The impact of humic acid additives on mineral metabolism of rabbits in the postnatal period of ontogenesis

Regulatory Mechanisms in Biosystems ◽

10.15421/022043 ◽

2020 ◽

Vol 11 (2) ◽

pp. 289-293

Author(s):

M. A. Rybalka ◽

L. M. Stepchenko ◽

O. O. Shuleshko ◽

L. V. Zhorina

Keyword(s):

Alkaline Phosphatase ◽

Humic Acid ◽

Blood Serum ◽

Bone Tissue ◽

Mineral Metabolism ◽

Histological Study ◽

Postnatal Period ◽

Control Group ◽

Copper And Zinc ◽

Experimental Group

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COMPARATIVE ASSESSMENT OF THE FUNCTIONAL PROPERTIES OF THE FOOD ADDITIVE «TOMATNAYA» OBTAINED BY VARIOUS TECHNOLOGIES

Известия вузов. Пищевая технология ◽

10.26297/0579-3009.2021.2-3.14 ◽

2021 ◽

Author(s):

Е.П. ВИКТОРОВА ◽

Е.В. ЛИСОВАЯ ◽

М.В. ЛУКЬЯНЕНКО ◽

Н.Н. КОРНЕН ◽

Е.В. КУЗЬМИНОВА ◽

...

Keyword(s):

Functional Properties ◽

Antioxidant Properties ◽

Food Additive ◽

The Body ◽

Innovative Technology ◽

Control Group ◽

Standard Diet ◽

Tomato Pomace ◽

White Rats ◽

Experimental Group

Для получения препаратов и добавок, обладающих антиоксидантными свойствами, представляют интерес выжимки томатов, образующиеся при производстве сока прямого отжима с предварительным отделением семян. Проведена сравнительная оценка эффективности функциональных свойств образцов пищевой добавки, полученных из выжимок томатов по различным технологиям: образец добавки 1 получен по технологии, включающей ИК-сушку выжимок, их охлаждение и измельчение; образец добавки 2 – по инновационной технологии, включающей дополнительную предварительную обработку выжимок в ЭМП СВЧ перед ИК-сушкой. Исследования проводили в течение 30 дней на трех группах лабораторных белых крыс: контрольной, животные которой получали стандартный рацион, и двух опытных, в которых животные помимо основного рациона получали при кормлении индивидуально 1 раз в сутки 2 г добавки образца 1 (1-я опытная группа) или образца 2 (2-я опытная группа). Установлено, что степень снижения концентрации ферментов печени АсАт и АлАт в крови животных 2-й опытной группы соответственно на 10,6 и 11,0% выше, чем в крови животных 1-й опытной группы. Степень снижения содержания диеновых коньюгатов, кетодиенов и малонового диальдегида в крови животных 2-й опытной группы была соответственно на 22,1; 10,1 и 12,7% выше по сравнению с аналогичными показателями животных 1-й опытной группы. Таким образом, доказано, что пищевая добавка «Томатная», выработанная по инновационной технологии, проявляет ярко выраженные антиоксидантные и гепатопротекторные свойства, что обеспечивает нормализацию биохимических процессов в организме, повышает его антиоксидантную защиту и препятствует разрушению мембран клеток. For the obtaining of preparations and additives with antioxidant properties, tomato pomace, formed during the production of direct-pressed juice with preliminary separation of seeds, is of interest. A comparative evaluation of the effectiveness of the functional properties of food additive samples obtained from tomato pomace using various technologies was carried out. The sample of additive 1 was obtained using a technology that includes IR drying of pomace, cooling and grinding; the sample of additive 2 was obtained using a technology that includes additional pretreatment of pomace in microwave EMF before IR drying. The studies were carried out for 30 days on three groups of laboratory white rats: a control group, the animals of which were given a standard diet, and two experimental ones, in which the animals, in addition to the main diet, were given individually once a day with 2 g of an additive of sample 1 (1st experimental group) or sample 2 (2nd experimental group). It was found that the degree of decrease in the concentration of liver enzymes AsAt and AlAt in the blood of animals of the 2nd experimental group was respectively 10,6 and 11,0% higher, than in the blood of animals of the 1st experimental group. The degree of decrease in the content of diene conjugates, ketodienes and malondialdehyde in the blood of animals of the 2nd experimental group was higher respectively by 22,1; 10,1 and 12,7% compared with similar indicators of animals of the 1st experimental group. Thus, it is proved that the food additive «Tomatnaya», developed by innovative technology, shows pronounced antioxidant and hepatoprotective properties, which ensures the normalization of biochemical processes in the body, increases its antioxidant protection and prevents the destruction of cell membranes.

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The effect of endodontic filling agents on the activity of the regeneration processes of bone tissue in the experiment

Journal of Medical Science ◽

10.20883/jms.2018.260 ◽

2018 ◽

Vol 87 (1) ◽

pp. 11-17

Author(s):

Iryna Han ◽

Khristina Musij-Sementsiv ◽

Volodymyr Zubachyk

Keyword(s):

Bone Tissue ◽

Antioxidant System ◽

Mineral Metabolism ◽

Blood Clot ◽

Malonic Dialdehyde ◽

Endodontic Treatment ◽

Bone Tissue Regeneration ◽

White Rats ◽

Apical Area ◽

Regeneration Processes

Introduction. Highly important is to regenerate the inflammatory destructive processes of bone tissue outside the apical area of the tooth that would optimize reparative bone formation, mineralization of tooth tissues, restoration of periodontal function and stability of tooth to occlusive loads, especially with acquired extensive root apex. Low efficiency of endodontic treatment led to the searching of the new osteotropic drugs of osteoconductive action and biorevitalization to stimulate the repairing and regeneration of tissues outside the apical area and dense obturation of the apex of the tooth root.Aim. of our research was to determine the dynamics of reparative processes in the bone tissue under the influence of drugs and compositions for endodontic treatment based on an analysis of indicators of a mineral metabolism, marker enzymes and an activity of antioxidant system and lipid peroxidation.Material and Methods. An experiment was conducted on 120 white rats. It was created a bone defect, which was filled with studied biomaterials or left with a blood clot. From the experiment the rats were taken out on 14 and 90 day. In homogenates of bone tissue it was examined the activity of lysosomal enzymes — alkaline (ALP) and acid (AP) phosphatases and the content of microelements — calcium and phosphorus. In the blood of rats it was studied the concentration of general protein, lipid peroxidation products — malonic dialdehyde (MDA) and the enzyme activity of antioxidant system — catalase (CAT) and superoxide dismutase (SOD).Results and conclusions. Results of conducted experimental research of the effect of endodontic filling materials on the activity of the regeneration processes of bone tissue show that proposed compositions based on hydroxyapatite and beta-tricalcium phosphate, due to their osteoconductive and biorevitalization qualities, promote more active stimulation of bone tissue regeneration processes compared to generally accepted drugs.

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Mineral substances in the bone tissue and blood of patients with osteoporosis

Medicinski pregled ◽

10.2298/mpns0910402a ◽

2009 ◽

Vol 62 (9-10) ◽

pp. 402-406 ◽

Cited By ~ 1

Author(s):

Zorica Ajdukovic ◽

Aleksandar Dimic ◽

Sasa Stankovic ◽

Nebojsa Krunic ◽

Ljiljana Aleksov ◽

...

Keyword(s):

Bone Tissue ◽

Mineral Content ◽

Alveolar Bone ◽

Mineral Metabolism ◽

Control Group ◽

Endocrine Disorders ◽

Metabolic Disturbances ◽

Dental Prostheses ◽

Mineral Substances ◽

Biochemical Analyses

Introduction. Osteoporosis is a very common disorder of skeleton, which can be caused by various metabolic disturbances, malnutrition, immobilization, endocrine disorders, bone marrow diseases, connective tissue disorders, and, in some cases, by inappropriate therapy. The aim of this investigation was to evaluate the relationships between the mineral content in the alveolar bone and serum osteoporosis in chosen patients. Material and methods. The study included an experimental and a control group of patients who were submitted to internal and prosthodontic therapy. The plan of therapy was to extract anticipated parodonthopathic teeth, taking samples of the alveolar bone and its analysis by spectrometry. At the same time the biochemical analyses of the blood were performed. Results. Before the therapy, due to negative remodeling, values of mineral content in decalcified bone tissue and serum were significantly lower than in the control group. After the therapy, the quality of the bone tissue was improved, with increased contents of mineral substances. Discussion and conclusion. Disorders of mineral metabolism are very important for osteoporosis. This disorder may involve entire skeleton, especially stomatoghnatic complex. Therefore osteoporosis is one of very important illnesses in dentistry, especially in prosthodontics, because of the supporting nature of bone tissue for all types of dental prostheses.

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A Discrimination Based on Punishment

Quarterly Journal of Experimental Psychology ◽

10.1080/17470215208416601 ◽

1952 ◽

Vol 4 (1) ◽

pp. 27-45 ◽

Cited By ~ 31

Author(s):

James A. Dinsmoor

Keyword(s):

The Other ◽

Control Group ◽

Experimental Session ◽

Group 4 ◽

White Rats ◽

The Difference ◽

Avoidance Reactions ◽

Experimental Group ◽

Rate Of Response

Twelve white rats learned to press a bar or lever when this act was intermittently followed by pellets of food. Once a stable rate of pressing had been established, the animals were subjected to electric shock as a punishment for each response during alternate five minute periods within the experimental session. A difference in rates during the safe and punished phases was manifested both by the experimental group (8 rats), who were provided with a light as a signal when it was safe to respond, and also, contrary to expectation, by the control group (4 rats), who continued in darkness throughout the session. The differential responding by the control group was greatly reduced, however, when the duration of each phase was reduced from five minutes to one. The investigation was then continued to determine the role of some of the other factors in the situation. Increasing the severity of the shock increased the difference between the light and the dark rates. Withholding the pellets of food reduced the overall rates but did not affect the light-dark discrimination. Withholding the shock, on the other hand, permitted an increase in rate and caused the animals to lose their discrimination; when food had previously been withheld, however, so that the rate of response was relatively low, this deterioration in the discrimination was not as rapid as before. Restoration of the discrimination training under a lower hunger drive confirmed the finding that the formation of the discrimination was quite rapid and showed that the level of drive did not affect the proportionality between the rates in the light and in the darkness. These findings were interpreted by comparing the current procedure with those previously used in studies of avoidance reactions and conflict.

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Radiographic, macromorphological and hematological evaluation of hydroxyapatite ceramics with diﬀ erent physicochemical properties

Naukovij vìsnik veterinarnoï medicini ◽

10.33245/2310-4902-2020-154-1-140-152 ◽

2020 ◽

pp. 140-152

Author(s):

V. Chemerovsky

Keyword(s):

Composite Materials ◽

Bone Tissue ◽

Blood Clot ◽

Hematological Parameters ◽

Indirect Evidence ◽

External Jugular Vein ◽

Control Group ◽

Reparative Osteogenesis ◽

Hydroxyapatite Ceramics ◽

Experimental Group

Treatments of fragmentary fractures in case of loss regenerative potential of bone tissue require usingdiﬀ erent composite materials. Among all of them, ceramics based on synthetic hydroxyapatite and tricalcium phosphate are considered promising. The reparative osteogenesis in spongy and compact rabbit’s bone tissueusing 3 composite materials with diﬀ erent physicochemical propertieswas studied. A reparative osteogenesiscontrolled by monitoring clinical, radiologic and macromorphologic parameters. Composite materials inﬂ uence on the organism was studied by determining the dynamics of hematological parameters throughout the study period. To realize this scheme was formed a 3 experimental groups and one control group of rabbits. The created defects of animals of ﬁ rst experimental group were ﬁ lled out with GT + α-TKF-500, thedefects of second oneanimals group ﬁ lled out with GTlKl-2, and defects ofthird oneanimals group ﬁ lled out with GTlKg-700. The GTlKl-700materials were doped with silicon. In control animals, bone defects were allowed to heal under a blood clot. During the studies, all animals were located in the same environment conditions. A blood samples for hematological investigation was taken from the external jugular vein before anesthesia and at the 3rd, 7th, 14th, 21st and 42nd day after surgery. X-ray examination was performed on 14th, 21st, and 42nddays.The animals were removed from the experiment on 21stand 42nd days. The samples were examined by macromorphologic method. By radiologic examination was found that composite materials had osteoconductive properties except the silicondoped example which hadosteoinductive properties. The samples of compact bone tissue of 1st and 3rd experimental groups characterized by formation of punctate osteosclerosis with a compacted contour of the periosteumon the 42nd day. But the bone samples of 2nd experimental group had compacted contours of the periosteum only. However, in the cancellous bone tissue on the 42nd day in the animals of the 1st experimental group developed point osteosclerosis, which visualized as individual granules of the composite.But in the animals of 2nd group thewhite spot which was outlined shape and homogeneous structure at the defect was found. The bone samples of 3rd animals group shoved a clearly limited point osteosclerosisforming were was found ceramic granules. In case using ofGT + α-TKF-500, a strong connection of granules from the formed bone tissue and without any periosteal growthswas noted. Using GTlKl-2 places of defects were identical to not injured sites of radial bones. Using GTlKg-700 shows that bone defect is ﬁ lled to the level of the plane of the bone surface and covered with a periosteum without visible growths. The granules of the composite are evenly distributed in the regenerate and associated with bone tissue. The analysis of hematological parameters did not reveal any fundamental diﬀ erences, but the use of hydroxyapatite implants is not accompanied, in contrast to spontaneous reparative osteogenesis, the development of leukocytosis, which indicates a moderate course of its inﬂ ammatory reaction. But the latter is accompanied by a platelet reaction, the most pronounced when using implants, which is probably due to the inﬂ uence of platelet factors and is indirect evidence of early osteoblastic reaction. Key words: fractures, rabbits, erythrocytes, leukocytes, platelets, hemoglobin.

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Influence of the supplement «Press-Acid» on protein-mineral metabolism and resistance of piglets

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.15421/nvlvet8364 ◽

2018 ◽

Vol 20 (83) ◽

pp. 320-324 ◽

Cited By ~ 1

Author(s):

N.V. Chеrniy ◽

E.V. Matsenko ◽

Yu.A. Shchepetilnikov ◽

Yu. V. Maslak ◽

O.S. Machula ◽

...

Keyword(s):

Blood Serum ◽

Mineral Metabolism ◽

The Body ◽

Control Group ◽

Immunological Parameters ◽

Oxidative Reaction ◽

Experimental Animals ◽

Growth Energy ◽

First Time ◽

Experimental Group

The aim of this study was to determine the effect of supplement «Press-Acid» on some parameters of mineral and protein metabolism and resistance of piglets. The study was done in condition «Meta» on two groups of piglets of the F-1 (BWхL) genotype. There were determined indexes of bacterial and lysocim activity of blood serum, calcium and phosphorus in piglets at the age of 21–30–45–75 days. Effect of supplement on the growth rate, morbidity and safety of pigs were revealed. We used hygienic, zootechnical, hematological, biochemical, immunological and statistical methods of analysis. The influence of «Press-Acid», which consist of formic, lactic, citric and fumaric acids, was established for the first time. The concentration of calcium in blood serum of experimental group of piglets were lower than in animals of control group. The level of phosphorus increased in blood serum of experimental animals on 11.83% (30 day), on 3.74% (45%), on 17.65% (75 day) respectively. Concentration of hemoglobin and the amount of erythrocytes increased on 11.8% and on 1.82% (30 day) in animals that feed the supplement. There were established the increase of viability of the pigs with gastro-intestinal diseases which were given the supplement in comparison with that, who did not feed it. Severity of diseases was determined using the Melenberg coefficient which did not higher than 1.2–2.4 score, it was lower on 6,0–9,3 unit than in control group. Using the supplement allowed to increase the intensity of growth of piglets by 3.7% (45 day) and 9.4% (75 day) respectively. Concentration of total protein, albumines and globulins increased on 8.66%, 9.90%, 7.3% respectively. The activity of alaninaminotransphaerasae in the blood serum of experimental animals were lower on 3.45% than in control piglets. Lysocym and bacterial activity of blood serum were higher in experimental group of piglets. Feeding piglets with addition supplement Press Acid promotes higher grow rates in experimental animals, that can be explained increases of oxidative-reducing processes in them organism. Feeding piglets with acidifier «Press-Acid», «Nifulin», zinc oxide, probiotics «Lactisan Complex» promotes growth energy, increase of morphological, biochemical and immunological parameters which indicates a higher level of oxidative reaction in the body. A low coefficient of Melenberg is recorded in the piglets at the age of 1 day. Safety was within 92.1–97.0%. That indicates a more successful elimination of complexes of protein, mineral substances, humoral protection in blood of experimental group of animals.

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DYNAMICS OF HAEMATOLOGICAL AND FUNCTIONAL PARAMETERS OF PEKIN DUCK BLOOD DURING ADMINISTRATION OF ORGANIC SELENIUM PREPARATION

Veterinary Science Today ◽

10.29326/2304-196x-2018-2-25-64-68 ◽

2018 ◽

pp. 64-68

Author(s):

Ye. O. Anisimova ◽

V. V. Pronin ◽

L. V. Kletikova ◽

N. N. Yakimenko

Keyword(s):

Total Protein ◽

Color Index ◽

Mineral Metabolism ◽

Pekin Duck ◽

Control Group ◽

Axillary Vein ◽

Functional Parameters ◽

Organic Selenium ◽

Age Related ◽

Experimental Group

Age-related dynamics of hematological and functional parameters of Pekin duck blood during administration of organic selenium preparation DAFS-25k (at a 1.3 mg/kg dose) was presented. In order to study morphological parameters from the age of one day old to 120 days old blood was collected from axillary vein of birds prior to feeding each 15 days in the morning. To assess hematological parameters RBC and WBC count was performed, hemoglobin contents and hematocrit value were determined and color index of blood was calculated. The experiment demonstrated gradual increase in these parameters in the experimental and control groups. Birds from the experimental group demonstrated higher parameters than those of the control group. It was determined that hemoglobin contents was 9.86% higher in the experimental group in comparison with the control group due to selenium administration. The blood color index in this group was also higher (р ≤ 0.05). Biochemical blood analysis included the following parameters: total protein, albumin, uric acid, glucose, calcium, phosphorus, ALT and AST transaminase. The test results showed that during all age periods total protein and albumin contents in ducks of the experimental group exceeded those of the control group. The selenium-containing preparation had a positive influence on the carbohydrate metabolism ensuring high level of metabolic processes in birds, contributed to optimal calcium to phosphorus ratio which positively influenced mineral metabolism and development of the locomotor system. Obtained AST and ALT values in both groups are not beyond reference values which indirectly confirms absence of toxic effect on ducks’ organism if selenium is administered at the specified doses. It was determined that DAFS-25k has not affected hematological and functional parameters of Pekin duck blood and it contributed to mineral metabolism correction, reduction of stress factor effect within critical periods of development and improvement of the excretory system function.

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Influence of the preparation “Bendamin” on the indicators of antioxidant protection of rat myocardium in experimental modeling of heart failure

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.32718/nvlvet9518 ◽

2019 ◽

Vol 21 (95) ◽

pp. 98-101

Author(s):

I. S. Varkholiak ◽

B. V. Gutyj

Keyword(s):

Intraperitoneal Administration ◽

Clinical Signs ◽

Free Radical Oxidation ◽

Feed Additives ◽

Control Group ◽

Standard Diet ◽

Antioxidant Protection ◽

Diene Conjugates ◽

Radical Oxidation ◽

Experimental Group

The aim of this study was to investigate the effect of Bendamin on the antioxidant status of rats in experimental doxorubicin-induced cardiomyopathy. The studies were performed on white sexually mature young male of Vistar line rats weighing 180–200 g, which were kept on a standard diet of the Institute Vivarium of the State Research Institute of Veterinary Drugs and Feed Additives. Animals were divided into 3 groups of 6 animals in each: control group – intact animals; experimental group R1, in which animals were simulated with doxorubicin-induced cardiomyopathy by intraperitoneal administration of doxorubicin at a dose of 2.5 mg/kg 3 times a week for two weeks; experimental group R2, in which animals were injected with the drug “Bendamin” at a dose of 20 mg/kg after injection of doxorubicin. The data obtained indicate that the introduction into the experimental animals of the experimental group of doxorubicin is accompanied by intensification in the myocardium of the processes of free radical oxidation. The increase in diene conjugates was found to be 32.7% and TBK-active products increased by 37.6% compared to the control group of rats. Doxorubicin was also found to cause inhibition of the glutathione system of the animal's antioxidant protection after administration to rats. After administration of the drug “Bendamin”, rats of the second experimental group were suppressed with lipid peroxidation under the conditions of doxorubicin intoxication. In the rats of the second experimental group, the level of intermediates and end products is likely to decrease, so in the rat myocardial homogenate the level of diene conjugates decreased by 16.8% and the level of TBA-active products by 20.8% compared to the first experimental group. who had clinical signs of cardiomyopathy caused by doxorubicin administration. The use of the drug “Bendamin” in rats of the second experimental group contributed to the enhancement of the enzymatic and non-enzymatic element of the antioxidant system, protecting the structural and functional integrity of cell bioembranes. The results of the studies enrich the pharmacological characteristics of the drug “Bendamin”, indicate its sufficiently clear protective effect on the myocardium in experimental doxorubicin cardiomyopathy and is a convincing proof of the feasibility of the drug in veterinary practice.

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