Influence of preparation of silver nanoparticles in carriers based on polymer/inorganic hybrids on the mineral composition of chicken eggs

The solution to the problem of reducing the use of antibiotics in the production of edible eggs is possible through the development and use of alternative bactericidal preparations, including those based on nanosilver. Obtaining biocompatible and biodegradable polymer/inorganic carriers of nanosilver provides for the study of its cumulative qualities which determine the safety of edible chicken eggs. The study investigated the mineral composition of edible eggs when feeding Hy-Line W36 laying hens solutions of the nanosilver preparation in carriers based on polymer/inorganic hybrids (AgNPs/SPH) given in the concentrations of 0, 1.0, and 2.0 mg/L of water (0, 0.2 and 0.4 mg/hen per day) three times at 10 day intervals. Oral administration to laying hens of an aqueous solution of silver nanoparticles in carriers based on polymer/inorganic hybrids at doses of 0.2 and 0.4 mg per hen per day in a dose-dependent manner increased the silver content and did not significantly affect the content of copper, zinc, iron, and lead in the eggshell. The preparation of silver nanoparticles did not affect the content of silver, copper, zinc, iron and lead in the albumen and yolk of chicken eggs after the first and second application, and after the third treatment of laying hens contributed to an increase in the silver content in the egg albumen and yolk but did not affect the content in them of copper, zinc, iron and lead. A single feeding of a solution of a preparation of nanosilver in carriers based on polymer/inorganic hybrids to hens at doses of 0.2 and 0.4 mg per hen per day after 10 days, contributed to a significant increase in the yolks of chicken eggs due to a decrease in the albumen and eggshell. The second and third application of nanosilver to poultry in the indicated doses contributed to a decrease in its proportion in the albumen and in the yolk due to a significant increase in its proportion in the shell. Selective accumulation of in-shell silver can be a promising means of improving the safety and security of chicken eggs when they are microbially contaminated. The results of using nanosilver based on polymer/inorganic hybrids in laying hens can be the basis for the development of methods for increasing the bactericidal properties of the shell and the safety of edible eggs.

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Incurred Arsenic Residues in Chicken Eggs

Journal of Food Protection ◽

10.4315/0362-028x-57.3.218 ◽

1994 ◽

Vol 57 (3) ◽

pp. 218-223 ◽

Cited By ~ 12

Author(s):

DAN J. DONOGHUE ◽

HERMAN HAIRSTON ◽

CAROL V. COPE ◽

MARY J. BARTHOLOMEW ◽

DAVID D. WAGNER

Keyword(s):

Laying Hens ◽

Dependent Manner ◽

Refrigerated Storage ◽

Withdrawal Period ◽

Arsanilic Acid ◽

Elemental Arsenic ◽

Egg Components ◽

Chicken Eggs ◽

Dose Dependent ◽

Whole Egg

Arsanilic acid and roxarsone were fed to laying hens at elemental arsenic concentrations of 14, 28, 56 or 112 ppm for 10 weeks followed by a 2-week withdrawal period. Arsenic residues in egg components of laying hens that were fed either control or diets treated with organic arsenicals were determined weekly by atomic absorption. Arsenic concentrations in eggs were also determined after either 0, 2 or 4 weeks of refrigerated storage (4°C). Arsenic residues in both yolk and albumen increased in a dose-dependent manner although the amount of arsenic was much higher (95% of total) in yolk. Arsenic concentrations increased within 1 week of treatment, and the highest amounts were obtained between the second and fourth week for yolk samples and by the first week for albumen samples, except in the 14-ppm doses where highest amounts were reached by the middle of the treatment period. Hens treated with 112 ppm arsenic from arsanilic acid produced eggs with arsenic residues exceeding the 500 ppb Food and Drug Administration whole egg tolerance level. Eggs subjected to refrigerated storage did not have increased arsenic concentrations in yolk, although, for a few treatments, residues increased in albumen.

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Biofabricated Fatty Acids-Capped Silver Nanoparticles as Potential Antibacterial, Antifungal, Antibiofilm and Anticancer Agents

Pharmaceuticals ◽

10.3390/ph14020139 ◽

2021 ◽

Vol 14 (2) ◽

pp. 139

Author(s):

Mohammad Azam Ansari ◽

Sarah Mousa Maadi Asiri ◽

Mohammad A. Alzohairy ◽

Mohammad N. Alomary ◽

Ahmad Almatroudi ◽

...

Keyword(s):

Fatty Acids ◽

Silver Nanoparticles ◽

Anticancer Agents ◽

Sem Analysis ◽

Dependent Manner ◽

Anticancer Activities ◽

Anticancer Efficacy ◽

Hct 116 ◽

Hct 116 Cells ◽

Dose Dependent

The current study demonstrates the synthesis of fatty acids (FAs) capped silver nanoparticles (AgNPs) using aqueous poly-herbal drug Liv52 extract (PLE) as a reducing, dispersing and stabilizing agent. The NPs were characterized by various techniques and used to investigate their potent antibacterial, antibiofilm, antifungal and anticancer activities. GC-MS analysis of PLE shows a total of 37 peaks for a variety of bio-actives compounds. Amongst them, n-hexadecanoic acid (21.95%), linoleic acid (20.45%), oleic acid (18.01%) and stearic acid (13.99%) were found predominately and most likely acted as reducing, stabilizing and encapsulation FAs in LIV-AgNPs formation. FTIR analysis of LIV-AgNPs shows some other functional bio-actives like proteins, sugars and alkenes in the soft PLE corona. The zone of inhibition was 10.0 ± 2.2–18.5 ± 1.0 mm, 10.5 ± 2.5–22.5 ± 1.5 mm and 13.7 ± 1.0–16.5 ± 1.2 against P. aeruginosa, S. aureus and C. albicans, respectively. LIV-AgNPs inhibit biofilm formation in a dose-dependent manner i.e., 54.4 ± 3.1%—10.12 ± 2.3% (S. aureus), 72.7 ± 2.2%–23.3 ± 5.2% (P. aeruginosa) and 85.4 ± 3.3%–25.6 ± 2.2% (C. albicans), and SEM analysis of treated planktonic cells and their biofilm biomass validated the fitness of LIV-AgNPs in future nanoantibiotics. In addition, as prepared FAs rich PLE capped AgNPs have also exhibited significant (p < 0.05 *) antiproliferative activity against cultured HCT-116 cells. Overall, this is a very first demonstration on employment of FAs rich PLE for the synthesis of highly dispersible, stable and uniform sized AgNPs and their antibacterial, antifungal, antibiofilm and anticancer efficacy.

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Synthesis, Characterization, and Optimization of Green Silver Nanoparticles Using Neopestalotiopsis clavispora and Evaluation of Its Antibacterial, Antibiofilm, and Genotoxic Effects

The EuroBiotech Journal ◽

10.2478/ebtj-2021-0020 ◽

2021 ◽

Vol 5 (3) ◽

pp. 109-122

Author(s):

Tuğba Kahraman ◽

Safiye Elif Korcan ◽

Recep Liman ◽

İbrahim Hakkı Ciğerci ◽

Yaser Acikbas ◽

...

Keyword(s):

Silver Nanoparticles ◽

Nuclear Ribosomal Dna ◽

Diffusion Method ◽

Dependent Manner ◽

Transmission Electron ◽

Infrared Spectrophotometer ◽

Average Size ◽

Novel Applications ◽

Dose Dependent

Abstract Silver nanoparticles (AgNPs) have been used in a variety of biomedical applications in the last two decades, including antimicrobial, anti-inflammatory, and anticancer treatments. The present study highlights the extracellular synthesis of silver nanoparticles AgNPs using Neopestalotiopsis clavispora MH244410.1 and its antibacterial, antibiofilm, and genotoxic properties. Locally isolated N. clavispora MH244410.1 was identified by Internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA. Optimization of synthesized AgNPs was performed by using various parameters (pH (2, 4, 7, 9 and 12), temperature (25, 35 and 45 °C), and substrate concentration (0.05, 0.1, 0.15, 0.2 and 0.25 mM)). After 72 hours of incubation in dark conditions, the best condition for the biosynthesis of AgNPs was determined as 0.25 mM metal concentration at pH 12 and 35 °C. Fungal synthesized AgNPs were characterized via spectroscopic and microscopic techniques such as Fouirer Transform Infrared Spectrophotometer (FTIR), UV-Visible Spectroscopy, and Transmission Electron Microscopy (TEM). The average size of the AgNPs was determined less than 60 nm using the TEM and Zetasizer measurement system (measured in purity water suspension). The characteristic peak of AgNPs was observed at ~414 nm from UV-Vis results. Antibacterial and genotoxic activity of synthesized AgNPs (0.1, 1, and 10 ppm) were also determined by using the agar well diffusion method and in vivo Somatic Mutation and Recombination Test (SMART) in Drosophila melanogaster. AgNPs exhibited potential antimicrobial activity against all the tested bacteria (Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa) except Escherichia coli in a dose-dependent manner. AgNPs did not induce genotoxicity in the Drosophila SMART assay. 79.33, 65.47, and 41.95% inhibition of biofilms formed by P. aeruginosa were observed at 10, 1, and 0.1 ppm of AgNPs, respectively. The overall results indicate that N. clavispora MH244410.1 is a good candidate for novel applications in biomedical research.

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Toxicity, bioaccumulation and biotransformation of glucose capped silver nanoparticles in green microalgae Chlorella vulgaris

10.21203/rs.3.rs-33984/v1 ◽

2020 ◽

Author(s):

Stefania Mariano ◽

Elisa Panzarini ◽

Maria Dias Inverno ◽

Nikolaos Voulvoulis ◽

Luciana Dini

Keyword(s):

Silver Nanoparticles ◽

Chlorella Vulgaris ◽

Waste Treatment ◽

Sea Urchins ◽

Aquatic Organism ◽

Consumer Products ◽

Toxic Substances ◽

Dependent Manner ◽

Green Microalgae ◽

Dose Dependent

Abstract BackgroundSilver nanoparticles (AgNPs) are one of the most widely used nanomaterials in consumer products. When discharged into the aquatic environment AgNPs can cause toxicity to aquatic biota, through mechanisms that are still under debate, thus rendering the NPs effects evaluation a necessary step. Different aquatic organism models, i.e. microalgae, mussels, Daphnia magna, sea urchins and Danio rerio, etc. have been largely exploited for NPs toxicity assessment. On the other hand, alternative biological microorganisms abundantly present in nature, i.e. microalgae, are nowadays exploited as a potential sink for removal of toxic substances from the environment. Indeed, the green microalgae Chlorella vulgaris is one of the most used microorganisms for waste treatment.ResultsWith the aim to verify the possible involvement of C. vulgaris not only as a model microorganism of NPs toxicity but also for the protection toward NPs pollution, we used these microalgae to measure the AgNPs biotoxicity and bioaccumulation. In particular, to exclude any toxicity derived by Ag+ ions release, green chemistry synthesised and Glucose coated AgNPs (AgNPs-G) were used. C. vulgaris actively internalised AgNPs-G whose amount increases in a time and dose-dependent manner. The internalised NPs, found inside large vacuoles, were not released back into the medium, even after 1 week, and did not undergo biotransformation since AgNPs-G maintained their crystalline nature. Biotoxicity of AgNPs-G causes an exposure time and AgNPs-G dose-dependent growth reduction and a decrease in chlorophyll-a amount.ConclusionsThese results confirm C. vulgaris as a biomonitoring organism and also suggest it as a bioaccumulating microalgae for possible use in the environment protection.

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Silver Nanoparticles Decrease the Viability of Cryptosporidium parvum Oocysts

Applied and Environmental Microbiology ◽

10.1128/aem.02806-15 ◽

2015 ◽

Vol 82 (2) ◽

pp. 431-437 ◽

Cited By ~ 21

Author(s):

Pamela Cameron ◽

Birgit K. Gaiser ◽

Bidha Bhandari ◽

Paul M. Bartley ◽

Frank Katzer ◽

...

Keyword(s):

Silver Nanoparticles ◽

Cryptosporidium Parvum ◽

Silver Ions ◽

Protozoan Parasite ◽

Dependent Manner ◽

Oocyst Wall ◽

Content Type ◽

Chlorine Disinfection ◽

High Concentrations ◽

Dose Dependent

ABSTRACTOocysts of the waterborne protozoan parasiteCryptosporidium parvumare highly resistant to chlorine disinfection. We show here that both silver nanoparticles (AgNPs) and silver ions significantly decrease oocyst viability, in a dose-dependent manner, between concentrations of 0.005 and 500 μg/ml, as assessed by an excystation assay and the shell/sporozoite ratio. For percent excystation, the results are statistically significant for 500 μg/ml of AgNPs, with reductions from 83% for the control to 33% with AgNPs. For Ag ions, the results were statistically significant at 500 and 5,000 μg/ml, but the percent excystation values were reduced only to 66 and 62%, respectively, from 86% for the control. The sporozoite/shell ratio was affected to a greater extent following AgNP exposure, presumably because sporozoites are destroyed by interaction with NPs. We also demonstrated via hyperspectral imaging that there is a dual mode of interaction, with Ag ions entering the oocyst and destroying the sporozoites while AgNPs interact with the cell wall and, at high concentrations, are able to fully break the oocyst wall.

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Anticancer Potential of L-Histidine-Capped Silver Nanoparticles against Human Cervical Cancer Cells (SiHA)

Nanomaterials ◽

10.3390/nano11113154 ◽

2021 ◽

Vol 11 (11) ◽

pp. 3154

Author(s):

Rajmohamed Mohammed Asik ◽

Chidhambaram Manikkaraja ◽

Karuppusamy Tamil Surya ◽

Natarajan Suganthy ◽

Archunan Priya Aarthy ◽

...

Keyword(s):

Cervical Cancer ◽

Silver Nanoparticles ◽

Microscopic Analysis ◽

Dependent Manner ◽

Apoptotic Pathway ◽

Ic50 Value ◽

Cervical Cancer Cells ◽

Microscopic Studies ◽

Human Cervical Cancer Cells ◽

Dose Dependent

This study reports the synthesis of silver nanoparticles using amino acid L-histidine as a reducing and capping agent as an eco-friendly approach. Fabricated L-histidine-capped silver nanoparticles (L-HAgNPs) were characterized by spectroscopic and microscopic studies. Spherical shaped L-HAgNPs were synthesized with a particle size of 47.43 ± 19.83 nm and zeta potential of −20.5 ± 0.95 mV. Results of the anticancer potential of L-HAgNPs showed antiproliferative effect against SiHa cells in a dose-dependent manner with an IC50 value of 18.25 ± 0.36 µg/mL. Fluorescent microscopic analysis revealed L-HAgNPs induced reactive oxygen species (ROS) mediated mitochondrial dysfunction, leading to activation of apoptotic pathway and DNA damage eventually causing cell death. To conclude, L-HAgNPs can act as promising candidates for cervical cancer therapy.

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Madhuca longifolia embedded silver nanoparticles attenuates diethylnitrosamine (DEN) -induced renal cancer via regulating oxidative stress

Current Drug Delivery ◽

10.2174/1567201817666200910154301 ◽

2020 ◽

Vol 17 ◽

Author(s):

Deepika Singh ◽

Ekta Yadav ◽

Vikas Kumar ◽

Amita Verma

Keyword(s):

Silver Nanoparticles ◽

Renal Cancer ◽

Safety Concern ◽

Treated Group ◽

Dependent Manner ◽

Protective Effects ◽

Antineoplastic Effect ◽

Tnf Α ◽

Madhuca Longifolia ◽

Dose Dependent

Objective: Madhuca longifolia has been used for the treatment of renal cancer. Therefore, the current study describes the protective effects of biofabricated silver nanoparticles (MLAgNPs) using Madhuca longifolia aqueous leaves extract against diethylnitrosamine (DEN) induced renal cell carcinoma (RCC) in rats. Methods: Animals were categorized into five groups and treated with doses of silver nanoparticles for 16 weeks. Antineoplastic effect in renal cancer was dose dependent to control the macroscopical variations when compared to DEN induced group. Significant changes were observed in biochemical parameters and dose graded improvement in the level of antioxidants parameters were accountable for its protective nature. Result: Silver nanoparticles in dose dependent manner was effective to modify the raised levels of pro-inflammatory cytokines and inflammatory mediators during renal cancer. Alteration in renal histopathology were also detected in the silver nanoparticles treated group, which show its safety concern. Biofabricated silver nanoparticles (MLAgNPs) using Madhuca longifolia can convey significant chemo-protective effect against renal cancer by suppressing the IL-6, TNF-α and IL-1β by nuclear factor-kappa B (NF-κB) pathway. Conclusion: Our outcomes implicates that biofabricated MLAgNPs exhibited a chemoprotective potential in the prevention and intervention of RCC.

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The in Vitro Effect of Polyvinylpyrrolidone and Citrate Coated Silver Nanoparticles on Erythrocytic Oxidative Damage and Eryptosis

Cellular Physiology and Biochemistry ◽

10.1159/000493460 ◽

2018 ◽

Vol 49 (4) ◽

pp. 1577-1588 ◽

Cited By ~ 6

Author(s):

Zannatul Ferdous ◽

Sumaya Beegam ◽

Saeed Tariq ◽

Badreldin H Ali ◽

Abderrahim Nemmar

Keyword(s):

Oxidative Stress ◽

Silver Nanoparticles ◽

Antimicrobial Agents ◽

Annexin V ◽

Drug Carriers ◽

Cellular Protein ◽

Dependent Manner ◽

Vitro Effect ◽

Dose Dependent

Background/Aims: Silver nanoparticles (AgNPs) are increasingly used as antimicrobial agents and drug carriers in various biomedical fields. AgNPs can encounter erythrocytes either directly following intravenous injection, or indirectly via translocation from the site of administration. However, information regarding the pathophysiological effects and possible mechanism of action of AgNPs on the erythrocytes are still inadequately studied. Thus, the aim of our study was to investigate the mechanism underlying the effect of coating and concentration of AgNPs on mouse erythrocytes in vitro. Methods: We studied the interaction of polyvinylpyrrolidone (PVP) and citrate (CT) coated AgNPs (10 nm) at various concentrations (2.5, 10, 40 µg/ml) with mouse erythrocytes in vitro using various techniques including transmission electron microscopy (TEM), hemolysis, and colorimetric measurement of markers of oxidative stress comprising malondialdehyde (MDA), reduced glutathione (GSH), and catalase (CAT). Intracellular calcium (Ca2+) was determined using Fura 2AM fluorescence. Annexin V was quantified using ELISA and the caspase 3 was determined both flurometrically and by western blot technique. Results: Following incubation of the erythrocytes with AgNPs, both PVP- and CT- AgNPs induced significant and dose - dependent increase in hemolysis. TEM revealed that both PVP- and CT- AgNPs were taken up by erythrocytes. The erythrocyte susceptibility to lipid peroxidation measured by MDA was significantly increased in both PVP-and CT- AgNPs. The concentration of GSH and CAT activity were significantly decreased by both types of AgNPs. Additionally, PVP- and CT- AgNPs significantly increased intracellular Ca2+ in a dose -dependent manner. Likewise, the concentration of the cellular protein annexin V was significantly and dose - dependently enhanced by both types of AgNPs. Furthermore, PVP- and CT- AgNPs induced significant increase in calpain activity in incubated erythrocytes. Conclusion: We conclude that both PVP- and CT- AgNPs causes hemolysis, and are taken up by erythrocytes. Moreover, we demonstrated that AgNPs induces oxidative stress and eryptosis. These findings provide evidence for the potential pathophysiological effect of PVP-and CT- AgNPs on erythrocyte physiology.

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Toxicity, Bioaccumulation and Biotransformation of Glucose-Capped Silver Nanoparticles in Green Microalgae Chlorella vulgaris

Nanomaterials ◽

10.3390/nano10071377 ◽

2020 ◽

Vol 10 (7) ◽

pp. 1377 ◽

Cited By ~ 1

Author(s):

Stefania Mariano ◽

Elisa Panzarini ◽

Maria D. Inverno ◽

Nick Voulvoulis ◽

Luciana Dini

Keyword(s):

Silver Nanoparticles ◽

Chlorella Vulgaris ◽

Waste Treatment ◽

Sea Urchins ◽

Aquatic Organism ◽

Consumer Products ◽

Toxic Substances ◽

Dependent Manner ◽

Green Microalgae ◽

Dose Dependent

Silver nanoparticles (AgNPs) are one of the most widely used nanomaterials in consumer products. When discharged into the aquatic environment AgNPs can cause toxicity to aquatic biota, through mechanisms that are still under debate, thus rendering the nanoparticles (NPs) effects evaluation a necessary step. Different aquatic organism models, i.e., microalgae, mussels, Daphnia magna, sea urchins and Danio rerio, etc. have been largely exploited for NPs toxicity assessment. On the other hand, alternative biological microorganisms abundantly present in nature, i.e., microalgae, are nowadays exploited as a potential sink for removal of toxic substances from the environment. Indeed, the green microalgae Chlorella vulgaris is one of the most used microorganisms for waste treatment. With the aim to verify the possible involvement of C. vulgaris not only as a model microorganism of NPs toxicity but also for the protection toward NPs pollution, we used these microalgae to measure the AgNPs biotoxicity and bioaccumulation. In particular, to exclude any toxicity derived by Ag+ ions release, green chemistry-synthesised and glucose-coated AgNPs (AgNPs-G) were used. C. vulgaris actively internalised AgNPs-G whose amount increases in a time- and dose-dependent manner. The internalised NPs, found inside large vacuoles, were not released back into the medium, even after 1 week, and did not undergo biotransformation since AgNPs-G maintained their crystalline nature. Biotoxicity of AgNPs-G causes an exposure time and AgNPs-G dose-dependent growth reduction and a decrease in chlorophyll-a amount. These results confirm C. vulgaris as a bioaccumulating microalgae for possible use in environmental protection.

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A Comprehensive Evaluation of Peripheral Analgesic and Antipyretic Activities of Divalent Metal Complexes of Indomethacin

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v16i2.35254 ◽

2018 ◽

Vol 16 (2) ◽

pp. 173-178

Author(s):

Abhijit Sukul ◽

Sabiha Chowdhury ◽

Saikat Kumar Poddar ◽

Sajal Kumar Saha ◽

SM Abdur Rahman

Keyword(s):

Acetic Acid ◽

Comprehensive Evaluation ◽

Cobalt Complex ◽

Divalent Metal ◽

Manganese Complexes ◽

Dependent Manner ◽

Antipyretic Activity ◽

Copper Zinc ◽

Dose Dependent ◽

Divalent Metal Complexes

Comparative peripheral analgesic and antipyretic activities of copper, zinc, cobalt and manganese complexes of indomethacin were studied. Peripheral analgesic effect of the complexes was evaluated by following acetic acid-induced writhing inhibition method in mice whereas antipyretic activity was assessed by Brewer’s yeastinduced pyrexia in rat model. At 10- and 20-mg/kg doses, the zinc-indomethacin complex produced significant writhing inhibition (p<0.001) by 67.35% and 77.55% whereas cobalt-indomethacin showed writhing inhibition by 61.22% and 64.63% in a dose dependent manner respectively, which were prominent compared to that of standard indomethacin (61.90% and 72.27% inhibition respectively). Moreover, the zinc-indomethacin complex exerted significant antipyretic activity at both 10- and 20-mg/kg dose by reducing body temperature from 100.38°F to 97.8°F (4th hour) and 100.36°F to 98.21°F (4th hour), respectively that were comparable to standard paracetamol. From this study, it can be said that, with further pharmacological and toxicological profiling of the zinc and cobalt complex of indomethacin, it may pave the way of discovering new drug entity.Dhaka Univ. J. Pharm. Sci. 16(2): 173-178, 2017 (December)

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