scholarly journals АКТИВНІСТЬ ФЕРМЕНТІВ У ГЕПАТОПАНКРЕАСІ КОРОПА (CYPRINUS CARPIO L.) ЗА ДІЇ СУЛЬФАНІЛАМІДУ

2016 ◽  
Vol 6 (3) ◽  
pp. 377-382
Author(s):  
Y. N. Kurbatova ◽  
V. M. Mykhalskaia ◽  
L. V. Malyuga ◽  
L. V. Gayova
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Test Group ◽  
The Body ◽  
Total Protein Content ◽  
Urea Content ◽  
Low Concentrations ◽  
Significant Difference ◽  
Aquarium Water ◽  
Activity Of Enzymes

<p align="center"> </p><p>The results of studies of the effect of different concentrations of sulfonamides in the aquarium water on the activity of enzymes and protein metabolism in the hepatopancreas of carp yearlings were presented. It was found that the sulfanilamide added to the aquarium water at a concentration of 1.10; 3.15 and 6.30 mg / dm3 and 3 had no effect on the total protein content in the fish hepatopancreas during three days of exposure at first, second and third experimental groups compared with the control. It is proved that when the concentration of water in the aquarium sulfanilamide at a dose of 1.10 and 3.15 mg / dm3 in the hepatopancreas urea content compared with the control is not changed, while a dose of 6,30 mg / dm3 it had increased by 4%. Probably, such an increase in urea levels in the hepatopancreas of fish third test group associated with the activation of regulatory mechanisms in the body of carp to maintain a positive nitrogen balance and reduce the toxic effects of ammonia. We also did not reveal significant difference between the indices of glucose concentration in the hepatopancreas of carp experimental groups compared with the control. We founded that the activity of alanine aminotransferase and aspartate aminotransferase in hepatopancreas of carp experimental groups did not differ from controls, suggesting there was no effect of low concentrations of sulfonamides in the water after brief operation of the xenobiotic on the functional state of the hepatopancreas. Conducted experiments did not revealed changes in lipase activity as α-amylase and fish hepatopancreas in first, second and third experimental groups compared with the control. Their activity remained within the normal characteristic of the species and indicates that sulfanilamide virtually had no effect on the intensity of the hydrolytic processes in the intestines of fish. The performed investigations proved that carp yearlings were capable of adapting to minor concentrations of sulfonamides in water, as evidenced by its lack of effect on the total protein content, the concentration of glucose, urea and the activity of several enzymes in the hepatopancreas of fish.</p>

scholarly journals Investigating chemical parameters in hot dog sausages from Novi Sad market

2011 ◽  
Vol 65 (5-6) ◽  
pp. 385-397
Author(s):  
Nadezda Prica ◽  
Milan Baltic ◽  
Milica Zivkov-Balos ◽  
Zeljko Cupic ◽  
Zeljko Mihaljev
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Fat Content ◽  
Total Protein Content ◽  
Average Content ◽  
Chemical Parameters ◽  
Significant Difference ◽  
The Difference ◽  
Novi Sad ◽  
Hot Dog

Meat products play an important role in the modern diet. The production of cooked sausages comprises an important segment in the complex meat industry. Hot dogs are prepared according to production specifications and differences in the quality of products can therefore be expected. This paper presents a comparative analysis of chemical parameters in products of five different manufacturers at the Novi Sad market. Water content, fat, protein, sodium chloride, the relative protein content of connective tissue in meat protein (RCP) and ash were analyzed. The difference between the average contents of chemical quality parameters in hot dogs was examined. The average water content ranged from 56.29?3.30% to 61.44?4.08%. The average total fat content ranged from 17.60?3.07% to 20.66?4.65%. No statistically significant difference (p?0.05) was detected in the fat content of the products of manufacturers D and B, as well as C and B. Between the average fat content of the producers E and D the difference in fat content was found to be p?0.01. In all other cases, the difference between the average fat content was p?0.001. The average total protein content in hot dogs ranged from 11.77?1.08% and 15.41?1.82. No statistically significant difference (p?0.05) was detected between the average total protein content in hot dogs of manufacturers A and C. Between the average protein content of D and E meat manufacturers the statistically significant difference had a value of p?0.01, and the average protein content in hot dogs of all other manufacturers had a significant difference (p?0.001). The total protein content in all five manufacturers of hot dogs met the prescribed standard (10%). RCP content in the hot dogs of different producers ranged from 17.34?0.95% to 22.33?2.08%. No statistically significant difference (p?0.05) was detected in the average contents of RCP in hot dogs of manufacturers D and E and A and B. but a statistically significant difference was detected (p?0.001). between the average content of RCP in all other manufacturers of hot dogs. The average chloride content in hot dogs from different manufacturers ranged from 1.30?0.10% to 2.55%?0.16%. The average ash content in different hot dog producers ranged from 1.99?0.18% to 3.46?0.22%. The average content of chloride and ash in all five manufacturers was significantly different.

scholarly journals The effect of convulsive syndrome on the metabolic status and survival of patients with alcoholic delirium

2020 ◽  
pp. 47-50
Author(s):  
Volodymyr Zadorozhnyi
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Delirium Tremens ◽  
Young Patients ◽  
The Body ◽  
Metabolic Status ◽  
Total Protein Content ◽  
Daily Excretion ◽  
Somatic Disorders ◽  
Severity Of The Disease

The aim of the study was to study the effect of convulsive syndrome in patients with delirium tremens (DT) on the charac teristics of the metabolic status of the body, the severity of clinical manifes tations, and survival of people of different ages. The study involved 753 patients with DT at the age of 20 to 76 years. The metabolic status was studied. One indicator was the urea-creatinine serum index; its low level was considered a sign of anabolic trends in metabolism. Another indicator was the determination of the total protein content in the body by daily excretion of creatinine. It was found that patients with DT with convulsive syndrome had a higher score according to the severity of the disease according to the DRS scale, a longer period of mental disorders, the average number of somatic complications was higher. However, the survival rate of patients with DT with convulsive syndrome and patients with DT without convulsive manifestations was the same. To determine the causes of this, the metabolic status and age of surviving and deceased patients with DT were compared. The highest total protein content in the body is observed in surviving patients with DT with manifestations of convulsive syndrome. Surviving patients with DT with manifestations of convulsive syndrome had a significantly lower level of urea-creatinine index compared with survivors with DT without convulsive syndrome. Among all patients with DT, the average age of survivors with convulsive syndrome was the lowest, and the average age of deceased patients with convulsive syndrome was the highest. In patients with DT without convulsive manifestations, the age of surviving and dead patients did not differ. The age of surviving patients with DT with convulsive syndrome is significantly lower than that of surviving patients without convulsive manifestations. The presence of convulsive syndrome and associated metabolic changes in the body in patients with DT contributes to the survival of a younger part of patients. Key words: alcohol delirium, seizures, aggravation of mental and somatic disorders, anabolic state of metabolism, primary survival of young patients

Cytotoxic Effects of Paracetamol in Vitro: The Effects of an S9 Fraction

1996 ◽  
Vol 24 (5) ◽  
pp. 715-725
Author(s):  
Katarina Ruppová ◽  
Darina Slameñová ◽  
Ladislave Wsølová ◽  
Alena Gábelová ◽  
Maria Vargová
Keyword(s):  
Protein Content ◽  
Hela Cells ◽  
Total Protein ◽  
Total Protein Content ◽  
Leucine Incorporation ◽  
Short Term ◽  
Cytotoxic Effects ◽  
Significant Difference ◽  
S9 Fraction

The cytotoxic effects of short-term and long-term exposure of HeLa cells to paracetamol (acetaminophen) were assayed by total protein content reduction (microprotein assay) and by [14C]-L-leucine incorporation into macromolecular acid-insoluble cell fraction. The level of total protein content was followed over 72 hours and the level of [14C]-L-leucine incorporation over 24 hours, after paracetamol treatment. Statistical evaluation did not show a significant difference between results obtained by these two methods. In addition, the influence of S9 fraction on [14C]-L-leucine incorporation and the growth activity of paracetamol-treated HeLa cells were assayed. In these experiments, short-term paracetamol treatments (2 hours in phosphate buffered saline), were used. Statistical analysis of the data did not show an increase in paracetamol-induced cytotoxicity in the presence of the S9 fraction; on the contrary, a protective effect of S9 fraction on paracetamol-treated cells was found.

HEMATOLOGICAL PARAMETERS PUREBRED AND CROSSBRED CALVES

2016 ◽  
Vol 1 (3) ◽  
pp. 45-48
Author(s):  
Исхаков ◽  
Rishat Iskhakov
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Physiological State ◽  
White Blood Cells ◽  
The Body ◽  
Adaptive Plasticity ◽  
Total Protein Content ◽  
Black And White

The purpose of research is scientifically and practically prove the possibility of increasing the level of meat productivity purebred for young calves at the crossing of Black-motley cows with bulls of the breed Obrac. Research objects are the bulls of Black-motley breed and their hybrids I generation with Obrac breed. For the experience 4 groups of animals were formed: I and III – Black and white breed, II and IV – gobies Оbrac crossbreeds ½ x ½ black-and-white. Bulls of groups III and IV in the 2 months of age were castrated by open way. All experimental animals identical conditions of detention and feed have been established. To monitor the physiological state of an organism of young animals in the winter and in the blood of the summer, blood was taken from the jugular vein before feeding and watering, measured hemoglobin, alkaline reserve, the number of white blood cells, red blood cells, serum – total protein, protein fractions, calcium, phosphorus, , the activity of AST and ALT. Deviations from the physiological norm morphological parameters of blood in the experimental groups of calves during the experiment was not set. Total protein content in serum was higher in summer compared to winter. Increasing the total protein content have purebred calves was 4.1 g/l (5.3%), crossbred steers 3.3 g/l (4.2%), respectively castrates at 4.2 g/l (5.6%) and 4.9 g/l (6.5%). There is a trend increase of enzyme activity in the summer, due to a more active course of metabolism in the body during this season of the year. Purebred and crossbred young are characterized by high adaptive plasticity, as evidenced by morphological and biochemical indices of blood. All quantitative and qualitative changes in the blood were largely seasonal in nature and due to the influence of environmental conditions. Thus all interior change indicators do not go beyond the limits of the physiological norm.

Seromucoid Determination: Variables and Modifications

1960 ◽  
Vol 6 (3) ◽  
pp. 189-198 ◽  
Author(s):  
T M Asher ◽  
G R Cooper
Keyword(s):  
Environmental Factors ◽  
Protein Content ◽  
Perchloric Acid ◽  
Total Protein ◽  
Standard Procedure ◽  
Total Protein Content ◽  
Inflammatory Condition ◽  
Freezing And Thawing ◽  
Significant Difference

Abstract The technic of Winzler (1) for the determination of seromucoid has been studied to measure its reproducibility and the effect of certain environmental factors. Values obtained are fairly reproducible (a difference of 45 mg./l00 ml. BSAP between two specimens indicates a significant difference at the P = 0.05 level). Variables such as temperature, freezing and thawing, speed of addition of perchloric acid, and total protein content of serum have no practical influence on the final SM values. A modification in technic involving determinations on three different dilutions of a specimen gives differences greater than those found with the standard procedure for sera from patients with an inflammatory condition.

scholarly journals Influence of vitamin-mineral supplement on protein metabolism in rabbits’ organisms

2021 ◽  
Vol 4 (1) ◽  
pp. 3-6
Author(s):  
M. Fedorchenko
Keyword(s):  
Blood Plasma ◽  
Protein Content ◽  
Biochemical Parameters ◽  
The Body ◽  
Control Group ◽  
Total Protein Content ◽  
Mineral Supplement ◽  
Significant Difference ◽  
Positive Effect ◽  
Alt Activity

The purpose of the study is to study the influence of different vitamin-mineral supplement “Tekro” doses on biochemical parameters in blood plasma and liver of rabbits of New Zealand breed. The research methodology is based on research according to generally accepted research methods. Biochemical parameters were studied in rabbits’ organisms at 45, 60, 75 and 90 days of rearing with feeding of different doses of vitamin-mineral supplement “Tekro”, namely 3 %, 3.5 %, 4 %. The academic novelty is that nowadays the problem of the effect of different vitamin-mineral supplement “Tekro” doses on biochemical parameters in the blood and liver of rabbits has not been studied. Analyzing the indicators of the total protein content in the blood plasma of rabbits of the experimental groups and the control one for 90 days, it was noted that there was no significant difference in the indicators. In the liver of rabbits of the age from 45 to 90 days there was a tendency to increase the protein content due to the effects on the body of various doses of vitamin and mineral supplement “Tekro”. It has been established the positive effect of vitamin-mineral supplement “Tekro” on ALT activity of rabbits in experimental groups. At the age of 75 days, significantly higher ALT activity was recorded in rabbits of the 3rd experimental group by 35.8 % compared to animals in the control group.

Prediction of Human Acute Toxicity by the Hep G2/24-hour/Total Protein Assay, with Protein Measurement by the CBQCA Method

2005 ◽  
Vol 33 (3) ◽  
pp. 207-213 ◽  
Author(s):  
Paul J. Dierickx
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Human Toxicity ◽  
Hep G2 ◽  
Vitro Assay ◽  
Protein Assay ◽  
Lowry Method ◽  
Total Protein Assay

In our previously described Hep G2/24-hour/total protein assay, protein levels were measured by using the Lowry method. This assay was the best acute in vitro assay for the prediction of human toxicity within the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) study. In order to increase the MEIC data-base with a wider range of chemicals, we were interested in introducing the more practical 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde (CBQCA) method for the quantification of the total protein content. Therefore, we investigated whether the same good results for the prediction of acute human toxicity would be obtained with the CBQCA method. The cells were treated for 24 hours, then cytotoxicity was determined by measuring the total protein content with CBQCA. The results were quantified by using the PI50c: the concentration (in mM) of test compound required to reduce the total protein content measured with the CBQCA-method by 50% as compared to the control cells. The results were compared with the PI50, the corresponding value when the Lowry method was used. A relatively low correlation was observed between PI50 and PI50c, reflecting the large and unexpected, differences when using the two protein assays. However, when comparing the log PI50c with the human toxicity, a correlation coefficient of r2 = 0.761 ( n = 44) was obtained for exactly the same series of MEIC chemicals. This value is clearly higher than that for the Lowry method ( r2 = 0.695). Compared to the Lowry method originally used, the Hep G2/24-hour/CBQCA total protein assay has the additional important advantage that it can be very easily adapted for large-scale analyses with robotic systems, including the on-line calculation of the results.

Total protein content and protein synthesis within pre-elongation stage bovine embryos

1998 ◽  
Vol 50 (2) ◽  
pp. 139-145 ◽  
Author(s):  
J.G. Thompson ◽  
A.N.M. Sherman ◽  
N.W. Allen ◽  
L.T. McGowan ◽  
H.R. Tervit
Keyword(s):  
Protein Synthesis ◽  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Bovine Embryos

scholarly journals Surface topography of hydroxyapatite affects ROS17/2.8 cells response

2002 ◽  
Vol 16 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Adalberto Luiz Rosa ◽  
Márcio Mateus Beloti ◽  
Richard van Noort ◽  
Paul Vincent Hatton ◽  
Anne Jane Devlin
Keyword(s):  
Cell Proliferation ◽  
Protein Content ◽  
Surface Topography ◽  
Cell Morphology ◽  
Total Protein ◽  
Cell Attachment ◽  
Maxillofacial Surgery ◽  
Total Protein Content ◽  
Alp Activity ◽  
Powder Pressing

Hydroxyapatite (HA) has been used in orthopedic, dental, and maxillofacial surgery as a bone substitute. The aim of this investigation was to study the effect of surface topography produced by the presence of microporosity on cell response, evaluating: cell attachment, cell morphology, cell proliferation, total protein content, and alkaline phosphatase (ALP) activity. HA discs with different percentages of microporosity (< 5%, 15%, and 30%) were confected by means of the combination of uniaxial powder pressing and different sintering conditions. ROS17/2.8 cells were cultured on HA discs. For the evaluation of attachment, cells were cultured for two hours. Cell morphology was evaluated after seven days. After seven and fourteen days, cell proliferation, total protein content, and ALP activity were measured. Data were compared by means of ANOVA and Duncan’s multiple range test, when appropriate. Cell attachment (p = 0.11) and total protein content (p = 0.31) were not affected by surface topography. Proliferation after 7 and 14 days (p = 0.0007 and p = 0.003, respectively), and ALP activity (p = 0.0007) were both significantly decreased by the most irregular surface (HA30). These results suggest that initial cell events were not affected by surface topography, while surfaces with more regular topography, as those present in HA with 15% or less of microporosity, favored intermediary and final events such as cell proliferation and ALP activity.

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