HEMATOLOGICAL PARAMETERS PUREBRED AND CROSSBRED CALVES

2016 ◽  
Vol 1 (3) ◽  
pp. 45-48
Author(s):  
Исхаков ◽  
Rishat Iskhakov

The purpose of research is scientifically and practically prove the possibility of increasing the level of meat productivity purebred for young calves at the crossing of Black-motley cows with bulls of the breed Obrac. Research objects are the bulls of Black-motley breed and their hybrids I generation with Obrac breed. For the experience 4 groups of animals were formed: I and III – Black and white breed, II and IV – gobies Оbrac crossbreeds ½ x ½ black-and-white. Bulls of groups III and IV in the 2 months of age were castrated by open way. All experimental animals identical conditions of detention and feed have been established. To monitor the physiological state of an organism of young animals in the winter and in the blood of the summer, blood was taken from the jugular vein before feeding and watering, measured hemoglobin, alkaline reserve, the number of white blood cells, red blood cells, serum – total protein, protein fractions, calcium, phosphorus, , the activity of AST and ALT. Deviations from the physiological norm morphological parameters of blood in the experimental groups of calves during the experiment was not set. Total protein content in serum was higher in summer compared to winter. Increasing the total protein content have purebred calves was 4.1 g/l (5.3%), crossbred steers 3.3 g/l (4.2%), respectively castrates at 4.2 g/l (5.6%) and 4.9 g/l (6.5%). There is a trend increase of enzyme activity in the summer, due to a more active course of metabolism in the body during this season of the year. Purebred and crossbred young are characterized by high adaptive plasticity, as evidenced by morphological and biochemical indices of blood. All quantitative and qualitative changes in the blood were largely seasonal in nature and due to the influence of environmental conditions. Thus all interior change indicators do not go beyond the limits of the physiological norm.

2016 ◽  
Vol 18 (3(70)) ◽  
pp. 196-200
Author(s):  
A.N. Petrenko ◽  
L.L. Kushch

Probiotic «Evitaliya» is freeze–dried, specific strains of lactic acid microorganisms (Lactococcus lactis, Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus helveticus, Propionibacterium fredenreichii ssp shernanii), they contain vitamins B1, B2, B6, B12, A, E, C iron minerals, calcium, magnesium.The purpose of the study was to clarify the effect of the probiotic ferment «Evitaliya» on nonspecific resistance of kids zaanovskoy breed microclimate conditions according VNTP (for sheep and goat breeding enterprises): the room temperature 12–14 °C, relative humidity of 55 – 70%, the carbon dioxide content – 1.5 – 1.8 l/m3 and ammonia – 10 – 15mg/m3 air bacterial contamination 15 – 20 tys. KOE/m3. Implementation of this goal was decided to use hygienic (temperature, relative humidity, air velocity); hematological (red blood cells, white blood cells, hemoglobin), biochemical (total protein, albumin, globulin); immune (humoral factors – lysozyme and bactericidal activity of blood serum, cell – the neutrophil phagocytic activity), statisticsGetting results and their novelty: for the first time tested the new probiotic yeast «Evitaliya», with its goats orally at a dose of – 200 ml/head in the morning and evening, 100 ml.It was found that the use of «Evitaliya» promotes activation of redox processes in the body kids, stimulation of erythropoiesis: increase in hemoglobin concentration of 0.56% (P ≤ 0.05), the number of red blood cells to – 10.2% (P ≤ 0.05).Use of the drug is moderately activates metabolic processes in the body of kids: increases total protein content by 7.14% (P ≤ 0.05), globulin – by 1.76% (P ≤ 0.05). Use of the drug «Evitaliya» at the indicated doses helps stimulate natural resistance olrganіzmu kids: humoral protection indicators (BASK) rose by 1.52% (LASK) by 1.81%, the cell indices (FA) compared to the control were higher – 4.97%. 


Author(s):  
A. Abilov ◽  
A. Azhmyakov ◽  
I. Novgorodova ◽  
N. Bogolyubova

Purpose: to study hematological parameters of blood in bulls-producers of dairy breeds on the day of semen collection in the Udmurt Republic after a long winter period of operation, depending on the breeds, age and place of selection.Materials and methods. The work was performed at the Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst on the basis of AO "Udmurtplem" of the Udmurt Republic in the period from 2020 to 2021 on dairy bulls (n=20) aged 15-69 months, including a purebred Holstein breed of domestic selection (n=6), a Holstein black-and-white breed of European selection (the Netherlands, n=6), a black-and-white breed with blood transfusion on Holsteins on at the level of 94-98% (n=8). The content of white blood cells, red blood cells, hemoglobin and hematocrit, depending on age and breed, was studied on the ABC VET hematological analyzer on the day of taking the seed.Results. It was found that on average, in 20 bulls aged 15-69 months, the level of white blood cells was at the level of reference values of 8.8±0.25 x 109/l, red blood cells 10.3 x 1012/l, which is 50% more than the reference values. The concentration of hemoglobin is 128.0±2.92 g/l, hematocrit is on average 54.3%, with a norm of 24-46%. Breeding bulls at a reliable level, differing in age, showed that some animals had high indicators for red blood cells of 10.3±0.26 x 102, for hematocrit of 54.3% against 24-46% of reference values. Also, according to hematological indicators, there was a tendency to increase the concentration of hemoglobin and hematocrit in European-bred bulls.Conclusion. The study of the variability in hematological parameters depending on the selection showed that there is no significant difference in leukocytes and all indicators are at the level of reference values, and in erythrocytes more than 50% than the highest indicators. The highest hemoglobin values were at the level of 141-156 g/l instead of 128 g/l according to the highest reference values. Hematocrit also showed high max values in all groups in comparison with the reference values of 59-66% versus 46% in the norm. It is necessary when analyzing hematological blood parameters in addition to the average statistical indicators (M+m) also, monitor the variability (min-max) in order to obtain more objective information.


2016 ◽  
Vol 1 (2) ◽  
pp. 60-63
Author(s):  
Полищук ◽  
Sergey Polishchuk ◽  
Молянова ◽  
Galina Molyanova

The purpose of research is to increase protective and adaptive reactions, the service and the working potential of an organism of dogs through the use of DHQ. The article presents the results of an experiment to the effect of DHQ on the dynamics of hematological parameters of dogs blood. Dihydroquercetin – an active antioxidant, a natural scavenger of oxygen free radicals, hepatoprotective, has anti-inflammatory action due to limitations of the formalin edema and histamine, and inhibits the formation of serous fluid, pain killers, immunocorrectional properties. Due to the high complexing properties it displays the body of heavy metals, including radionuclides, it helps to restore the tone of blood vessels, normalization of the lipid spectrum of the blood and slows the development of atherosclerotic plaques. Investigations were carried out in Dogs State Service Ministry of I A of Russia zonal center in Samara Region by clinically healthy of dog breed German Shepherd, age 2-4 years with an average body weight of 30 kg on the background conditions and feeding, adopted by the enterprise. Dogs experimental group received Dihydroquercetin to 0.001 g/kg body weight dose once a day with food. When added to the basic diet Dihydroquercetin dog experimental group show an increase in indicators such as red blood cells – by 18.3% (p<0.01), hemoglobin – by 11.7% (p <0.01) hematocrit – 7.1% (p<0.01), white blood cells – by 9.1% (p<0.05) compared to control data. The research results give reason to believe that the use of dietary supplements in Dihydroquercetin in dogs diet dose 0.001 g/kg allows significantly increase the morphological and physiological indicators of animal organism. In this case a significant increase erythrocytes and hemoglobin and hematocrit indicates increased oxidative metabolism and function of the intensity of the blood in dogs treated with the drug, and increase in the blood indicates an increase in immune status.


Author(s):  
Volodymyr Zadorozhnyi

The aim of the study was to study the effect of convulsive syndrome in patients with delirium tremens (DT) on the charac teristics of the metabolic status of the body, the severity of clinical manifes tations, and survival of people of different ages. The study involved 753 patients with DT at the age of 20 to 76 years. The metabolic status was studied. One indicator was the urea-creatinine serum index; its low level was considered a sign of anabolic trends in metabolism. Another indicator was the determination of the total protein content in the body by daily excretion of creatinine. It was found that patients with DT with convulsive syndrome had a higher score according to the severity of the disease according to the DRS scale, a longer period of mental disorders, the average number of somatic complications was higher. However, the survival rate of patients with DT with convulsive syndrome and patients with DT without convulsive manifestations was the same. To determine the causes of this, the metabolic status and age of surviving and deceased patients with DT were compared. The highest total protein content in the body is observed in surviving patients with DT with manifestations of convulsive syndrome. Surviving patients with DT with manifestations of convulsive syndrome had a significantly lower level of urea-creatinine index compared with survivors with DT without convulsive syndrome. Among all patients with DT, the average age of survivors with convulsive syndrome was the lowest, and the average age of deceased patients with convulsive syndrome was the highest. In patients with DT without convulsive manifestations, the age of surviving and dead patients did not differ. The age of surviving patients with DT with convulsive syndrome is significantly lower than that of surviving patients without convulsive manifestations. The presence of convulsive syndrome and associated metabolic changes in the body in patients with DT contributes to the survival of a younger part of patients. Key words: alcohol delirium, seizures, aggravation of mental and somatic disorders, anabolic state of metabolism, primary survival of young patients


2020 ◽  
Vol 384 (2) ◽  
pp. 22-28 ◽  
Author(s):  
N. V. Gaponov ◽  
S. V. Svistunov ◽  
N. N. Bondarenko ◽  
I. A. Romanenko

The use of water with a modified isotopic composition in the diet of humans and animals, for example, with a reduced deuterium content, affects the rate of biological processes. In this regard, the targeted formation of the isotopic D / H gradient in the body can be used to increase its adaptive properties to external influences. Primates have a high anatomical and physiological similarity with humans, therefore, are an indispensable model for reproducing various pathological and toxic conditions in humans. The aim of the study was to study the effect of water with a low deuterium content on the hematological and biochemical blood parameters of rhesus monkeys and on the digestibility of nutrients of the diet. In the control group, primates consumed water with a natural regional content of deuterium equal to 150 mg / l, and in the experimental group - with reduced to 50 mg/l. The duration of the experiment was 35 days. The material for the study was venous blood, serum and feces of primates. Blood was drawn from monkeys before the use of deuterium depleted water and after the completion of the experiment. All blood samples (2.5-3.0 ml) were taken from the cubital or femoral veins of animals on an empty stomach and stabilized with a heparin solution. Laboratory studies of animal blood were performed on an automatic hematological analyzer (Beckman Coulter, USA) of the CoulterAcT 5diffCP brand. In order to study the effect of water with a reduced deuterium content on hematological parameters, we determined the number of red blood cells, white blood cells, platelets, hemoglobin concentration, hematocrit, the average volume of red blood cells, red blood cell anisocytosis, and erythrocyte sedimentation rate (ESR). The metabolism trial was divided into two periods. Preliminary - to exclude the influence of previous feeding and accustoming primates to cage conditions of keeping. Experimental - conducting a thorough accounting of consumed feed, excreted excrement. Feces were collected daily at the same time (morning and evening), weighed and ground in a mortar. At each collection, 50% of the homogenized mass was taken for analysis. The collected portions were stored in the refrigerator. After the end of the experimental (accounting) period in the collected feces, the initial moisture was determined by drying at 60-70 ℃ to constant weight. The resulting air-dry mass was thoroughly ground and analyzed. It has been found that the introduction of water with a reduced deuterium content into the diet of primates has a positive effect on feed conversion rate and blood biochemical parameters.


Author(s):  
Y. N. Kurbatova ◽  
V. M. Mykhalskaia ◽  
L. V. Malyuga ◽  
L. V. Gayova

<p align="center"> </p><p>The results of studies of the effect of different concentrations of sulfonamides in the aquarium water on the activity of enzymes and protein metabolism in the hepatopancreas of carp yearlings were presented. It was found that the sulfanilamide added to the aquarium water at a concentration of 1.10; 3.15 and 6.30 mg / dm3 and 3 had no effect on the total protein content in the fish hepatopancreas during three days of exposure at first, second and third experimental groups compared with the control. It is proved that when the concentration of water in the aquarium sulfanilamide at a dose of 1.10 and 3.15 mg / dm3 in the hepatopancreas urea content compared with the control is not changed, while a dose of 6,30 mg / dm3 it had increased by 4%. Probably, such an increase in urea levels in the hepatopancreas of fish third test group associated with the activation of regulatory mechanisms in the body of carp to maintain a positive nitrogen balance and reduce the toxic effects of ammonia. We also did not reveal significant difference between the indices of glucose concentration in the hepatopancreas of carp experimental groups compared with the control. We founded that the activity of alanine aminotransferase and aspartate aminotransferase in hepatopancreas of carp experimental groups did not differ from controls, suggesting there was no effect of low concentrations of sulfonamides in the water after brief operation of the xenobiotic on the functional state of the hepatopancreas. Conducted experiments did not revealed changes in lipase activity as α-amylase and fish hepatopancreas in first, second and third experimental groups compared with the control. Their activity remained within the normal characteristic of the species and indicates that sulfanilamide virtually had no effect on the intensity of the hydrolytic processes in the intestines of fish. The performed investigations proved that carp yearlings were capable of adapting to minor concentrations of sulfonamides in water, as evidenced by its lack of effect on the total protein content, the concentration of glucose, urea and the activity of several enzymes in the hepatopancreas of fish.</p>


2021 ◽  
Vol 44 (3) ◽  
pp. 210-221
Author(s):  
M. D. Olumide ◽  
A. O. Akinsoyinu ◽  
R. A. Hamzat

Competition exists between man and his livestock for conventional feed ingredients like maize. This has necessitated the search for alternative ingredients. Large quantities of cocoa bean shell are produced and wasted annually by farmers and associated processing industries in Nigeria. A total of one hundred and fifty day-old Anak broiler chicks were randomly allotted to five dietary treatments with 30 birds per treatment, replicated thrice, in a completely randomised design. The treatments were: A (0% CBS-control diet); B (5% RCBS); C (10% RCBS); D (15% RCBS) and E (20% RCBS). Birds were fed ad libitum throughout the experimental period of eight weeks. The feed intake and weight gained were monitored. Carcass analysis, serum and hematological parameters such as total protein, albumin, globulin, cholesterol, glucose, creatinine, red blood cell, white blood cells, hemoglobin and packed cell volume were determined. Significant differences were observed in the feed intake, the birds fed with the control diet A had the highest feed intake 4556.50gm compared with birds fed diet D and E with mean values of 4177.50gm and 4097.00gm respectively. The highest weight gain was obtained from birds on control diet (1952.00gm) and the lowest from birds with 20% RCBS (1550.00gm, diet E). As the level of inclusion of RCBS increased the feed conversion ratio value increased. The dressed out percentage ranged from 73.67% - 67.00%. Apparent variations in the values obtained for total protein, creatinine, red blood cells, hemoglobin and packed cell volume of the birds fed graded levels of RCBS were not significant. However, white blood cells and globulin values increased with increase in the level of CBS in the diet. RCBS could effectively replace up to 10% maize in the diets of broilers without a deleterious effect on broiler chicken.


2020 ◽  
Vol 36 (2) ◽  
pp. 215-223
Author(s):  
O.M. Altynbekov ◽  
◽  
A.V. Andreeva ◽  

The article reflects the results of changes in the biochemical and morphological parameters of blood, as well as the content of total protein and its fractions under the influence of immunostimulators in black-and-white cows and calves obtained from them. The aim of the study was to study the effect of the immunostimulators “Recombinant Bovine Interferon” and “Immunate” on the morphological and immunobiochemical blood parameters of pregnant cows and newborn calves. Studies were conducted on 45 pregnant cows divided into three groups based on the principle of analogues, and, subsequently, on 45 calves obtained from mother cows and divided into nine groups. Maternal cows during pregnancy and newborn calves were given immunostimulators in various combinations. In the blood of cows of the experimental groups, five days before calving, higher indicators of red blood cells and hemoglobin were registered in comparison with the control indicators. Also, in experimental cows, the content of total protein and globulins exceeded the indicators of the control group. Calves received from cows immunostimulated with “Recombinant Bovine Interferon” and who were given “Immunate” after birth had the smallest decrease in the number of red blood cells and white blood cells on the tenth day. After drinking colostrum in calves of experimental groups, the total amount of protein increased, especially there was an increase in the gamma-globulin fraction. For all periods of the study, the content of immunoglobulins A, M, G in the blood serum of calves of the control group was lower than that of calves of the experimental groups. The maximum difference between these indicators and the control was found in animals of the sixth and ninth groups.


2020 ◽  
Vol 12 ◽  
pp. 52-60
Author(s):  
O.A. Knyazhеchenko ◽  
◽  
I.A. Semenova ◽  
A.A. Mosolov ◽  
M.V. Frolova ◽  
...  

Aim. To study the feasibility of using feed additives containing powdered feed preparations with different lactulose content in the amount of 5 g/kg of extruded compound feed together with the main diet in terms of the effect on the quality indicators of raw materials and the natural resistance of the body of experimental rabbits. Materials and Methods. The effectiveness was evaluated after the control slaughter at the age of 150 days in the number of 5 heads from each group. The change in live weight was determined when the rabbits reached the age of 75, 105, 135 and 150 days by weighing on an electronic scale. The physiological state and resistance of rabbits were evaluated by hematological parameters. The obtained materials were processed by the methods of variation statistics. Results. The analysis of dynamics of changes of alive weight of animals showed that feed supplementation with lactulose had a positive impact on the growth of animals the live weight of rabbits in groups I and II were greater at 3.8 and 6.2% absolute increase in live weight during the period of experience increased by 5.3 and 7.9%, respectively, compared to control. At the same time, the effect of supplements on blood parameters should also be noted. In our studies, the rabbits of the experimental groups had an advantage over the rabbits of the control group in terms of the level of red blood cells by 0.94·1012 g/l (18.7%) and 1.12·1012 g/l; white blood cells - by 0.50·109 (7.2%) g/l and 0.59·109 g/l (8.5%), respectively. The level of hemoglobin in the blood of rabbits increased by the end of the experiment in the control group by 3.7%, in I – by 12.5%, in II – by 15.9%. Conclusion. Based on the presented results, it can be concluded that the data on quantitative changes in blood were due to the influence of feed additives, which contributed to an increase in the natural resistance of rabbits, and the best indicators were achieved by rabbits of the II experimental group.


1972 ◽  
Vol 14 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Zofia Osińska ◽  
Aleksandra Ziołecka

SUMMARYData on the protein content of the empty body were obtained for 44 young Polish Black-and-White Lowland bulls ranging from 10-day- old calves to bulls more than 400 kg live weight. The percentage of protein of the empty body was found to be practically constant (19–19·5%) over the weight range investigated. Less than a half (up to 48 %) of the total protein content was found in the edible parts of the carcass.


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