scholarly journals Determination of the parameters of acute toxicity of «Brovermectin-granulate» on earlings carp

2017 ◽  
Vol 19 (74) ◽  
pp. 20-23
Author(s):  
Yu. V. Loboiko ◽  
M. M. Danko ◽  
O. V. Krushelnytska ◽  
S. I. Kravets
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Oral Administration ◽  
General Condition ◽  
Lethal Dose ◽  
Toxic Substances ◽  
Starch Solution ◽  
Method Of Calculation ◽  
Anterior Intestine

The paper presents the results of research to determine the parameters of acute toxicity of «Brovermectin-granulate». The material for the study of acute toxicity were of carp earlings. In experiment used drug «Brovermectin-granulate» (1 g of the drug contains: active actiion substance ivermectin – 3.5 mg, tocopherol acetate – 20 mg). The introduction of the drug  was carried out orally using a probe in anterior intestine of fish. The drug was administered to fish in the form of a homogeneous suspension produced 1 % starch solution individually, in doses of 1000, 4000, 8000, 12000, 16000 and 20000 mg/kg of body weight. For settings acute toxicity determined the general condition and death of fish; for a dose of the drug (DL50) calculated by methods G. Kerber, H. Pershyn, least squares analysis of probit mortality curves for V.B. Prozorovskyj, means three points for B.M. Shtabskyj. At this stage of research it was found lethal (DL100) and maximum tolerated (DL0) dose of «Brovermectin-granulate» for earlings carp. As a result of studies found that 100% death of fish following the dose of 20000 mg/kg (DL100), and for the drug at a dose of 4000 mg/kg (DL0) set to survival of 100% fish. Established that the value of average lethal dose of «Brovermectin-granulate» for fish regardless of the method of calculation mainly coincided and were 10932,8–11200 mg/kg for earlings carp. Thus, the drug «Brovermectin-granulate» for oral administration to fish belongs to grade 4 toxicity – low toxic substances.

ACUTE TOXICITY OF OCTENIDINE HEXAFLUOROSILICATE

2021 ◽  
Author(s):  
I. O. Shyshkin ◽  
V. Yu. Anisimov ◽  
A. V. Nikitin ◽  
V. O. Gelmboldt
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Least Squares Method ◽  
Lethal Dose ◽  
Statistical Processing ◽  
Toxic Substances ◽  
White Rats ◽  
Relative Safety ◽  
Lethal Doses

The aim of the work. Determination of toxicometric characteristics of octenidine hexafluorosilicate (OHFS), characterized by significant pharmaceutical potential, in an acute experiment on rats by oral administration. Materials and Methods. A study of the acute toxicity of octenidine hexafluorosilicate was carry out on 42 male Wistar rats weighing 180–200 grams. The main criterion for quantifying the toxicity of octenidine hexafluorosilicate was LD50, which was determined using the least squares method. In addition, the following hazard indicators were calculated: 1/LD50 – median lethal dose (absolute toxicity), LD84/LD16 – the range of lethal doses (zone of acute toxic effect), 1/(LD50-S) – the total toxicity index and the S-function angle of inclination (variability of lethal doses). Statistical processing of the results was carry out using the «StatPlus 2009» software (AnalystSoft, USA, 2009). Results and Discussion. The results of the acute toxicity determination of octenidine hexafluorosilicate show that this compound, in the oral route of administration, belongs to the III class of toxicity for the human (slightly hazardous) and to the IV class of toxicity for the animals (white rats). Based on the variability of lethal doses, the studied hexafluorosilicate can be attribute to compounds that do not pose a high potential risk of the onset and development of poisoning. The calculated toxicity and hazard values of octenidine hexafluorosilicate show that it does not pose a particular danger to humans. Extrapolation to humans of acute toxicity parameters obtained in animals was determined using the coefficient of resistance to the species and is 132.15 mg/kg body weight. Conclusions. The results of determining the toxicometric characteristics of octenidine hexafluorosilicate in rats by oral administration allow to classify this compound as moderately toxic substances (LD50 = 555.05 mg / kg, toxicity class IV). The determined parameter of acute toxicity of OHFS is close to the LD50 values of other hexafluorosilicates known from the literature; relative safety and high caries-prophylactic and periodontal-protective efficacies of OHFS indicate the prospects for further studies of this compound.

scholarly journals Study of acute toxicity of the drug «Kolidev 8M» with a single intragastric injection in laboratory animals

2021 ◽  
pp. 44-48
Author(s):  
Roman Sachuk ◽  
Yaroslav Stravskyy ◽  
Bogdan Gutyj ◽  
Tetiana Velesyk ◽  
Orest Katsaraba ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Oral Administration ◽  
Veterinary Drug ◽  
Control Group ◽  
Intragastric Administration ◽  
Toxic Substances ◽  
Class Iii ◽  
Absolute Weight ◽  
Class Iv

«Kolidev 8M» (powder for oral use) is a veterinary drug used to treat ornamental birds (pheasants, peacocks) in diseases of the digestive tract caused by microorganisms sensitive to colistin. In the study of the drug «Kolidev 8M» for oral administration, along with the confirmation of therapeutic properties, it is necessary to determine the LD50 obtained in the process of studying acute toxicity. The aim of research. The aim of research was to determine the acute toxicity of the veterinary drug «Kolidev 8M» (powder for oral administration) under the conditions of intragastric administration to white mice. Materials and methods of research. To achieve this aim, an experiment was conducted on 114 males of nonlinear white mice kept under optimal conditions in the vivarium of DEVIE LLC (Rivne, Ukraine). In the first series of experiments on the principle of analogues was formed control and three experimental groups of 6 animals each (n=6). The drug in the form of a solution was administered once orally using a esophageal gastric tube in doses of 500,0; 2000,0 and 4000,0 mg/kg body weight by absolute weight of the drug. The animals of the control group were injected with distilled water. After taking into account the results of the first experiment in the next experiment, 6 experimental groups were formed – mice, which were administered the drug «Kolidev 8M» in the form of a solution in doses (by absolute weight of the drug) – 500,0; 1000,0; 1500,0; 2000.0; 2500,0; 3000,0; 3500 and 4000,0 mg/kg body weight, as well as the control group – animals that were injected with distilled water with a volume of 0.5 cm3 according to similar regulations (Zapadniuk, 1983; Kotsiumbas, 2005; Karkyshchenko & Hrachev, 2010). There were 6 animals in each group (n=6). After their death, a pathological autopsy was performed (Zharov A. et al., 2003). The average lethal dose of LD50 was calculated by the method of probit analysis by Prozorovsky V.B. Research results. According to the results of research, it was found that the LD50 of the drug «Kolidev 8M» (powder for oral administration) under the conditions of its single intragastric administration to male mice is 2024,72±232,45 mg/kg, LD10 – 392,87 mg/kg, LD16 – 751,56 mg/kg, LD84 – 3297,88 mg/kg, LD90 – 3656,57 mg/kg, LD100 – 3934,47 mg/kg body weight, respectively. According to the results of an acute toxicological experiment with intragastric administration of the drug «Kolidev 8M» to white male mice, LD50 was 2024,72±232,45 mg/kg body weight. This allows, according to toxicity, to refer this drug to class IV – low-toxic substances (LD50 501,0-5000,0 mg/kg body weight), and the degree of danger to class III – moderately safe substances (LD50 151,0-5000,0 mg/kg body weight). Conclusions and prospects for further research. The drug «Kolidev 8M» in terms of toxicity belongs to class IV – low-toxic substances, and the degree of danger to class III – moderately safe substances. Further studies will be the next stage of pre-registration trials aimed at studying the subacute toxicity of the drug «Kolidev 8M»

scholarly journals The determination of the minimal lethal dose of various toxic substances and its relationship to the body weight warm-blooded animals, together with considerations bearing on the dosage of drugs

1914 ◽  
Vol 87 (595) ◽  
pp. 319-330 ◽  
Keyword(s):  
Body Weight ◽  
Blood Volume ◽  
Body Surface ◽  
Lethal Dose ◽  
The Body ◽  
Toxic Substances ◽  
Production Distribution ◽  
Close Relationship ◽  
Series Of Experiments

In the course of investigations on the production, distribution, and rate of disappearance in the body of immune substances, we were occupied in 1908 and previous years with a series of experiments on agglutinins, and we arrived at conclusions pointing to their close relationship to the blood and blood-forming organs (1, 2). In association with these inquiries, one of us (G. D.), together with W. Ray, published a communication on the relation­ship between the blood volume and the distribution of agglutinins within the circulation (3). It was there shown that the concentration of this substance (agglutinin) in the blood after inoculation into an animal was proportional to the body surface of the animal concerned, and was thus approximately proportional to the two-thirds power of the weight. Hence was deduced the conclusion that the blood volume of the animals examined was proportional to their body surface.

scholarly journals Determination of toxicological characteristics of a complex antiemeric drug based on maduramycin and nicarbazine

2021 ◽  
pp. 37-43
Author(s):  
Roman Dotsenko ◽  
Yevheniia Vashchyk ◽  
Andriy Zakhariev ◽  
Andrii Zemlianskyi ◽  
Ekaterina Dotsenko ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Oral Administration ◽  
Adult Male ◽  
Guinea Pigs ◽  
Male Rats ◽  
Agent Based ◽  
White Rats ◽  
Single Oral Administration

The aim: to determine the parameters of acute toxicity of the preparative form of an antiemeric agent based on maduramycin and nicarbazine for white mice, white rats and guinea pigs with a single oral administration. Materials and methods. Determination of acute toxicity of the formulation by oral administration was performed on 48 adult male mice, 48 adult nonlinear male rats, 48 adult male guinea pigs. To conduct an experiment on the principle analogues were formed seven experimental and one control group, 6 animal each. The dose of the formulation was calculated individually based on body weight values. It should be noted that the total volume of the emulsion of the formulation administered orally is not exceeded 1.0 cm3per 100 gram b. w. Results. Toxicometric parameters of the formulation were calculated by the method of least squares for probit analysis of mortality curves. It was found that the LD50 of the preparative form of antiemeric agent for white mice for a single oral administration is 238.05±28.08 mg/kg, LD16 – 128.71 mg/kg, LD84 – 347.39 mg/kg, LD100 - 402, 06 mg/kg body weight, respectively. LD50 of the preparative form of antiemeric agent for white rats with a single oral administration is 260.51±28.83 mg/kg, LD16 – 148.39 mg/kg, LD84 – 372.65 mg/kg, LD100 – 428.71 mg/kg body weight, respectively. LD50 of the preparative form of antiemeric agent for guinea pigs for a single oral administration is 275±21.12 mg/kg, LD16 – 201.74 mg/kg, LD84 – 348.25 mg/kg, LD100 – 384.88 mg/kg body weight body respectively. Conclusions. According to SOU 85.2-37-736: 2011 "Veterinary drugs. Determination of acute toxicity” preparative form of a complex antiemeric agent based on maduramycin and nicarbazine on the degree of toxicity can be attributed to moderately dangerous substances (3rd hazard class)

scholarly journals Determination of acute toxicity parameters of “Zoodizin” disinfectant

2019 ◽  
Vol 2 (2) ◽  
pp. 41-44
Author(s):  
O. L. Nechyporenko ◽  
A. V. Berezovskyy ◽  
H. A. Fotina ◽  
R. V. Petrov ◽  
T. I. Fotina
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Oral Administration ◽  
White Male ◽  
Lethal Dose ◽  
Well Being ◽  
Female Rats ◽  
Male Rats ◽  
Laboratory Animals ◽  
Intragastric Administration

An important element in ensuring the epizootic well-being of the poultry industry is disinfection. Modern poultry farming requires a large number of effective disinfectants. It is known that the resistance of microorganisms to the effects of disinfectants is based on a genotypic mechanism. The nature of the formation of resistance to disinfectants and antiseptics is different than antibiotics. With regard to disinfectants, resistance is formed more slowly and the proportion of resistant strains in the population of microorganisms may not be high for a long time. This is due to different mechanisms of formation of resistance to antibiotics and disinfectants, in the first case – plasmid mechanism, in the second – chromosomal. However, increasing the resistance to the active substance in disinfectants can be widespread, so it is necessary to periodically rotate disinfectants. The goal of the work – to investigate the parameters of acute toxicity of the disinfectant biocide “Zodizin”. The studies were conducted in the laboratory of Veterinary Pharmacy and the Vivarium of Sumy National Agrarian University. The drug “Zodizine” contains: polyhexamethyleneguanidine hydrochloride – 21.0 %, alkylldimethylbenzylammonium chloride – 3.0 %. For toxicological examination of the disinfectant, healthy white male rats and white female rats weighing 200 ± 10 g 1.5 years of age were used. In the study of acute toxicity of animals observed daily, noted the general condition of the animals, features of their behavior. Studies have found that the toxic effect of the disinfectant “Zodizin” clinically manifested almost equally in both males and females. The average lethal dose for the rat female was 1000.0 ± 35.0 mg/kg body weight, males 1033.0 ± 34.3 mg/kg. Therefore, according to the classification of substances by toxicity, the drug by intragastric administration can be attributed to low-toxic substances. Observations on animals revealed that 1–3 hours after oral administration of the drug in a subtoxic dose in laboratory animals, shortness of breath and inhibition of the central nervous system were noted. Most of them died during the first day. Subsequent observations of the surviving animals indicated that their motor response was suppressed over the next 24–72 hours. Conclusions and prospects for further research: 1. It was found that the average lethal dose of the drug “Zodizin” with oral administration to rats-females was 1000.0 ± 35.0 mg/kg body weight, males – 1033.0 ± 34.3 mg/kg. 2. Experimental studies have proved that the disinfectant “Zodizin” according to GOST 12.1.007-76, belongs to the IV class of danger, that is, to the low-dangerous compounds, and according to GOST 12.1.07 – to the III class of hazard of substances and can be used for disinfection premises where animals and poultry are kept. Further, the sporoсide and corrosion properties of the “Zoodizin” biocide will be studied.

scholarly journals In Vivo Antiplasmodial Activities and Acute Toxicity Assessment of Two Plant Cocktail Extracts Commonly Used Among Southwestern Nigerians.

2021 ◽  
Author(s):  
Rachel Omagha ◽  
E.T. Idowu ◽  
C.G. Alimba ◽  
A.O Otubanjo ◽  
E.O. Agbaje
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Plasmodium Berghei ◽  
Lethal Dose ◽  
Toxicity Assessment ◽  
High Dose ◽  
Test Models ◽  
Level Of Significance

Abstract Discovering and developing the desired antimalarials continue to be a necessity especially due to treatment failures, drug resistance, limited availability and affordability of pharmaceutical antimalarials, costs and logistical problems especially in poor malarious countries. This study investigated the efficacies of two plant cocktails; CtA and CtB, selected based on their traditional usage. Activities of the cocktail extracts, chloroquine and pyrimethamine against Plasmodium berghei berghei were evaluated using the suppressive, curative and prophylactic test models, after oral and intraperitoneal acute toxicity determination of the plant cocktails in accordance with Lorke method. Data was analyzed using SPSS software version 23.0 with level of significance set at P<0.05. The median lethal dose was determined to be higher than 5000 mg/kg body weight orally for both CtA and CtB; and 316.23 mg/kg body weight intraperitoneally for CtA. Each cocktail exhibited high dose dependent Plasmodium berghei berghei inhibition which was 96.95%, 99.13% in the CtA800 mg/kg, CtB800 mg/kg doses in the curative groups respectively, 96.46%, 78.62% for CtA800mg/kg, CtB800mg/kg doses in the suppressive groups respectively, and 65.05%, 88.80% for CtA800mg/kg, CtB800mg/kg doses in the prophylactic groups respectively. Throughout the observation periods, the standard drugs, chloroquine phosphate and pyrimethamine maintained higher inhibitions up to 100%. These findings demonstrate that CtA and CtB possess good antimalarial abilities and calls for their development and standardization as effective and readily available antimalarial options. The acute toxicity results obtained underscore the importance of obtaining information on toxicities of medicinal plant remedies before their administration in both humans and animals.

scholarly journals Determination of acute toxicity parameters of the drug ‘MEGASTOP for dogs’ on white rats and mice

2020 ◽  
Vol 6 (2) ◽  
pp. 20-26
Author(s):  
O. L. Orobchenko ◽  
M. Ye. Romanko ◽  
M. O. Yaroshenko ◽  
I. O. Gerilovych ◽  
N. A. Zhukova ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Clinical Symptoms ◽  
Liver Volume ◽  
Male Rats ◽  
Toxic Substances ◽  
Main Experiment ◽  
Polyethylene Glycol 400 ◽  
White Rats ◽  
Rats And Mice

The experiments were performed on 58 males of nonlinear white rats 3–4 months old and weighing 180–200 g and 64 females of nonlinear white mice 2.5–3 months old and weighing 18–22 g. In the main experiment on rats, six experimental groups were formed, the animals of which were injected intragastrically with the drug ‘MEGASTOP for dogs’ (by absolute weight) in doses of 1,000.0, 2,000.0, 3,000.0, 4,000.0, 5,000.0, and 6,000.0 mg/kg body weight; in the main experiment on mice, seven experimental groups were formed, the animals of which were administered the drug in doses of 100.0, 500.0, 1,000.0, 1,500.0, 2,000.0, 2,500.0, and 3,000.0 mg/kg body weight. Control rats and mice were injected with 2.0 cm3 and 0.2 cm3 of polyethylene glycol-400, respectively. Clinical symptoms of poisoning with the drug ‘MEGASTOP for dogs’ of white rats (at doses of 2,000.0–6,000.0 mg/kg body weight) and mice (at doses of 1,000.0–3,000.0 mg/kg body weight) were refusals of food and water, loss of coordination, sitting in one place, a dose-dependent increase in depression with subsequent complete depression, lack of response to external stimuli and death on the first or fourth day after administration. During autopsy in rats and mice that died as a result of poisoning with the drug ‘MEGASTOP for dogs’, we recorded pallor of the mucous membranes of the mouth, trachea, pharynx, and esophagus; increase in heart volume, atrial blood supply; pulmonary hyperemia; uncoagulated blood; increase in liver volume, dark cherry color, flabby consistency; catarrhal inflammation of the mucous membrane of the small intestine. According to the results of determining the parameters of acute toxicity of the drug ‘MEGASTOP for dogs’ in the case of a single intragastric injection, LD50 for male rats is 3,384.98 ± 444.94 mg/kg, and for female mice — 2,025.88 ± 279.46 mg/kg body weight, which allows to classify it to class IV by the toxicity — low-toxic substances (LD50 — 501–5,000 mg/kg) and by the degree of danger to class III— moderately dangerous substances (LD50 — 151–5,000 mg/kg)

scholarly journals TOXICIDADE AGUDA DO INSETICIDA PARATION METÍLICO E DO BIOPESTICIDA AZADIRACTINA DE FOLHAS DE NEEM (Azadirachta indica) PARA ALEVINO E JUVENIL DE PACU (Piaractus mesopotamicus)

2004 ◽  
Vol 14 ◽  
Author(s):  
CLAUDINEI CRUZ ◽  
JOAQUIM G. MACHADO-NETO ◽  
MANOEL LIMA DE MENEZES
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Environmental Risk ◽  
Azadirachta Indica ◽  
Methyl Parathion ◽  
Juvenile Fish ◽  
Aquatic Environments ◽  
Piaractus Mesopotamicus ◽  
Neem Leaves

Os objetivos deste trabalho foram calcular a CL (I) 50-96h do inseticida organofosforado paration metílico e do biopesticida azadiractina para alevinos e juvenis de pacu ( P. mesopotamicus). Também pretendeu-se avaliar o efeito do peso corpóreo sobre a toxicidade aguda do paration metílico e do neem para o pacu, bem como classificar o risco ambiental do uso de paration metílico e do neem para o controle de parasitas e patógenos de pacu. Foram realizados dois experimentos, em condições laboratoriais, para a determinação da concentração letal (CL (I) 50-96h). A CL (I) 50-96h calculada do paration metílico foi de 3,97 mg/L para os alevinos e de 9,89 mg/L para os juvenis. Para a azadiractina foi de 1,20 mg/L para os alevinos e de 1,18 mg/L para os juvenis. As concentrações de 1,0 mg/L de paration metílico para os alevinos e de 7,5 mg/L para os juvenis e as de 0,29 e 0,59 mg/L de azadiractina não provocaram mortalidade nos animais expostos e podem ser utilizadas como referência em estudos de controle de parasitas em pacu . O paration metílico foi menos tóxico para os alevinos e para os juvenis de pacu do que a azadiractina, indicando a necessidade de cuidados com a utilização de extratos aquosos de neem no ambiente aquático. ACUTE TOXICITY OF THE INSECTICIDE METHYL PARATHION AND OF THE BIOPESTICIDE AZADIRACHTIN FROM NEEM LEAVES (Azadirachta indica) TO ALEVINE AND JUVENILE PACU (Piaractus mesopotamicus) Abstract The objectives of the present study were: to calculate the lethal concentration LC (I) 50-96 h of methyl parathion and of the biopesticide azadirachtin to alevine and juvenile pacu ( P. mesopotamicus). Also to determine the effect of body weight on acute toxicity of methyl parathion and of neem for pacu, as well as to classify the environmental risk of the methyl parathion and neem uses for the control of pacu parasites and pathogens. Two experiments were performed under laboratory conditions for the determination of the LC (I) 50-96 h. The LC (I) 50-96 h of methyl parathion was 3.97 mg/L for alevine and 9.89 mg/L for juvenile fish. The LC (I) 50-96 h of azadirachtin was 1.20 mg/L for alevine and 1.18 mg/L for juvenile pacu. Concentrations of 1.0 and 7.5 mg/L of methyl parathion and of 0.29 and 0.59 mg/L of azadirachtin for alevine and juvenile pacu, respectively, did not cause mortality in the exposed animals and can be used as references in studies on the control of parasites in pacu. Methyl parathion was less toxic to alevine and juvenile pacu than azadirachtin, indicating the need for careful utilization of aqueous neem extracts in aquatic environments.

Acute Oral Safety Study of Sodium Caseinate Glycosylated via Maillard Reaction with Galactose in Rats

2014 ◽  
Vol 77 (3) ◽  
pp. 472-479
Author(s):  
ARTURO ANADÓN ◽  
MARIA A. MARTÍNEZ ◽  
IRMA ARES ◽  
VICTOR CASTELLANO ◽  
MARIA R. MARTÍNEZ-LARRAÑAGA ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Maillard Reaction ◽  
Lethal Dose ◽  
Sodium Caseinate ◽  
Biological Properties ◽  
Female Rats ◽  
Weight Changes ◽  
Safety Study ◽  
Male And Female Rats

In order to potentially use sodium caseinate (SC) glycated with galactose (Gal) in the food industry as a new functional ingredient with proved technological and biological properties, an evaluation of oral acute toxicity has been carried out. An acute safety study with SC-Gal glycoconjugates in the Wistar rat with a single oral gavage dose of 2,000 mg/kg of body weight was conducted. The SC-Gal glycoconjugates were well tolerated; no adverse effects or mortality was observed during the 2-week observation period. No abnormal signs, behavioral changes, body weight changes, or alterations in food and water consumption occurred. After this period, no changes in hematological and serum chemistry parameters, organ weights, or gross pathology or histopathology were detected. It was concluded that SC-Gal glycoconjugates obtained via the Maillard reaction were well tolerated in rats at an acute oral dose of 2,000 mg/kg of body weight. The SC-Gal glycoconjugates have a low order of acute toxicity, and the oral 50% lethal dose for male and female rats is in excess of 2,000 mg/kg of body weight.

scholarly journals Research of acute toxicity, allergizing and local-irritative action of the veterinary drug “Yodozol”

2019 ◽  
pp. 54-58
Author(s):  
R. M. Sachuk ◽  
S. V. Zhyhalyuk ◽  
I. M. Lukyanik ◽  
M. S. Mandyhra ◽  
Ya. S. Stravsky ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Behavioral Responses ◽  
Physiological Parameters ◽  
Laboratory Animals ◽  
Veterinary Drug ◽  
Intragastric Administration ◽  
Toxic Substances ◽  
Treatment And Prevention ◽  
Mucous Membranes

The purpose of the work was to determine, in experiments on rodents, the parameters of acute toxicity, allergenic and locally irritative effects of iodine-containing uterine drug for the treatment and prevention of intrauterine infections of animals. Materials and methods. Preclinical studies of acute toxicity of “Yodosol” containing iodine and potassium iodide were performed on 90 white mice, 30 white outbred rats and 6 rabbits. Clinical, pharmacotoxicological and statistical methods were used. Results of work. It has been found that at intragastric administration in experimental rats and mice, DL50 values exceed 8,000 mg/kg body weight and have no effect on the behavioral responses and physiological parameters of laboratory animals. It has been investigated that “Yodosol” aerosol has no local toxic and irritant effects on the skin and mucous membranes of laboratory animals (rabbits). Conclusions. The use of the drug «Yodosol», in doses above 8,000 mg/kg body weight, does not affect the behavioral responses and physiological parameters of laboratory animals. The drug has no local toxic and irritant effects on the skin and mucous membranes. According to the requirements of SOU 85.2-37-736:2011 and GOST 12.1.007-76, the newly developed drug “Yodosol” belongs to low-toxic substances — 4 toxicity classes

Sign in / Sign up

Export Citation Format

Share Document