Diversity of <i>Bacillus thuringiensis</i> strains isolated from coffee plantations infested with the coffee berry borer <i>Hypothenemus hampei</i>

Coffea spp. are tropical plants used for brewing beverages from roasted and grounded seeds, the favorite drink in the world. It is the most important commercial crop plant and the second most valuable international commodity after oil. Global coffee trade relies on two Coffea species: C. arabica L. (arabica coffee) comprising 60% and C. canephora (robusta) comprising the remaining 40%. Arabica coffee has lower productivity and better market price than robusta. Arabica coffee is threatened by disease (i.e., coffee leaf rust), pests [i.e., Hypothenemus hampei or coffee berry borer (CBB) and nematodes], and susceptibility to climate change (i.e., drought and aluminum toxicity). Plant biotechnology by means of tissue culture inducing somatic embryogenesis (SE) process, genetic transformation, and genome editing are tools that can help to solve, at least partially, these problems. This work is the continuation of a protocol developed for stable genetic transformation and successful plant regeneration of arabica coffee trees expressing the Bacillus thuringiensis (Bt) toxin Cry10Aa to induce CBB resistance. A highly SE line with a high rate of cell division and conversion to plants with 8-month plant regeneration period was produced. To validate this capability, gene expression analysis of master regulators of SE, such as BABY BOOM (BBM), FUS3, and LEC1, embryo development, such as EMB2757, and cell cycle progression, such as ETG1 and MCM4, were analyzed during induction and propagation of non-competent and highly competent embryogenic lines. The particle bombardment technique was used to generate stable transgenic lines after 3 months under selection using hygromycin as selectable marker, and 1 month in plant regeneration. Transgenic trees developed fruits after 2 years and demonstrated expression of the Bt toxin ranging from 3.25 to 13.88 μg/g fresh tissue. Bioassays with transgenic fruits on CBB first instar larvae and adults induced mortalities between 85 and 100% after 10 days. In addition, transgenic fruits showed a seed damage lower than 9% compared to 100% of control fruits and adult mortality. This is the first report on stable transformation and expression of the Cry10Aa protein in coffee plants with the potential to control CBB.

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Capture of hypothenemus hampei ferrari (coleoptera, scolytidae) in response to trap characteristics

Scientia Agricola ◽

10.1590/s0103-90162006000600010 ◽

2006 ◽

Vol 63 (6) ◽

pp. 567-571 ◽

Cited By ~ 15

Author(s):

Flávia Cloclet da Silva ◽

Maurício Ursi Ventura ◽

Lauro Morales

Keyword(s):

Environmental Conditions ◽

Field Experiments ◽

The State ◽

Low Density ◽

Hypothenemus Hampei ◽

Coffee Berry ◽

Release Rates ◽

Coffee Berry Borer ◽

Coffee Plantations ◽

Coffee Oil

Traps lured with semiochemical have been proposed to manage the coffee berry borer, coffee planting is however made under several environmental conditions that may affect the efficacy of traps. Several trap designs and variations were proposed and, therefore, a series of field experiments was carried out to capture coffee berry bores in traps disposed in a low density coffee plantations in the Northern part of the State of Paraná, Brazil. Traps were composed of plastic bottles (2 L) with a window (13 × 18 cm). Ethanol (E), Methanol (M) and coffee oil alone did not improve the capture when transparent traps with a 2 mm hole in the vial dispenser were used; traps lured with E : M mixtures (1:1, 1:2, 1:3) caught similar and higher number of insects than the control. There was synergism with the mixing of E and M; the addition of coffee oil to the mixtures did not improve the capture. Trap lured with E : M (1:1) mixtures at 342, 400, 428 and 710 mg caught similar numbers of insects. Transparent green trap, transparent trap and red trap lured with 642 mg day-1 of the E + M (1:1) mixture caught similar numbers of insects. Interaction between colors (transparent green, transparent and red) and semiochemical release rates (540, 720 and 1100 mg day-1) was observed. Transparent green trap, when lured with 720 mg day-1 of the 1 : 1 E : M mixture caught 2.3 and 4.4 times more insects than 540 and 1100 mg day-1; and caught 3.2 times more insects than transparent and red trap at the same release rates.

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Parasitismo na população da broca-do-café Hypothenemus hampei (Ferrari) (Coleoptera: Scolytidae), pelo parasitoide Cephalonomia stephanoderis Betrem (Hymenoptera: Bethylidae)

EntomoBrasilis ◽

10.12741/ebrasilis.v7i3.402 ◽

2014 ◽

Vol 7 (3) ◽

pp. 178-182 ◽

Cited By ~ 1

Author(s):

Moisés Santos De Souza ◽

Alexandre Almeida e Silva ◽

César Augusto Domingues Teixeira ◽

José Nilton Medeiros Costa

Keyword(s):

Biological Control ◽

Hypothenemus Hampei ◽

Coffee Berry ◽

Coffee Berry Borer ◽

Coffee Plantations ◽

Pest Infestation ◽

Coffee Beans ◽

Coffee Plants ◽

Cephalonomia Stephanoderis ◽

America Latina

A broca-do-café, Hypothenemus hampei (Ferrari) é responsável por grandes prejuízos em plantios de café, causando perdas de produtividade aos cafeicultores e danos quantitativos e qualitativos aos grãos. O controle biológico da praga tem sido principalmente à base de fungos entomopatogênicos. Em Rondônia, Brasil, as informações acerca dos parasitoides de H. hampei são escassas. O parasitoide Cephalonomia stephanoderis Betrem é um inimigo natural da broca do café e já é utilizado como agente de controle biológico em países da África e da América Latina. Constatou-se a presença desse parasitoide em cafezais de Rondônia. O objetivo do presente estudo foi investigar os níveis de ocorrência e parasitismo do parasitóide em uma plantação de café no município de Ouro Preto D’Oeste, Estado de Rondônia. Foram analisados semanalmente frutos de café coletados diretamente das plantas e caídos sobre o solo, de janeiro a dezembro de 2004. Observou-se a ocorrência do parasitoide durante os meses de maior infestação da praga. Durante o período estudado, a taxa de parasitismo nos diferentes microambientes variou de 2 a 24%. Parasitism in the Population of the Coffee Berry Borer Hypothenemus hampei (Ferrari) (Coleoptera: Scolytidae) by the Parasitoid Cephalonomia stephanoderis Betrem (Hymenoptera: Bethylidae) Abstract. The coffee berry borer, Hypothenemus hampei (Ferrari) attacks coffee plantations, greatly decreasing the production of coffee plants, causing quantitative and qualitative damage to the grains. Biological control of this pest has focused mostly on entomopathogenic fungi. In the state of Rondonia, studies on parasitism levels of the parasitoids of H. hampei are scarce. The parasitoid Cephalonomia stephanoderis Betrem is a natural enemy of the coffee berry borer and is already used as an agent of biological control in countries of Africa and Latin America. The objective of the present study was to investigate the occurrence and parasitism levels of the parasitoid in a coffee plantation in the municipality of Ouro Preto D’Oeste, State of Rondonia. Coffee beans collected directly from the plants and on the ground were analyzed weekly from January to December 2004. Parasitoid occurred in months of the highest pest infestation. During the studied period, parasitism rate in different microenvironments ranged from 2 to 24%.

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Three Species of Weeds Enhance The Population of Predator and Parasitoid of Coffee Berry Borers

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v10i1.12076 ◽

2018 ◽

Vol 10 (1) ◽

pp. 229-236

Author(s):

Cucu Daniati ◽

Sri Karindah ◽

Retno Puspitarini

Keyword(s):

Natural Enemies ◽

Single Species ◽

Hypothenemus Hampei ◽

Coffee Berry ◽

Ageratum Conyzoides ◽

Coffee Plantation ◽

Coffee Berry Borer ◽

Coffee Tree ◽

Coffee Plantations ◽

Arachis Pintoi

Flowering weeds can serve as refugia for natural enemies. In coffee plantation, they might has the potential to attract predator and parasitoid of Coffee Berry Borer (CBB), Hypothenemus hampei Ferr. (Coleoptera: Scolytidae). The aim of this research was to elucidate the influence of weeds to the presence of predator dan parasitoid and their impact to the population of CBB. The research was conducted at Ngantang, Malang Regency and in Laboratory of Pest, Faculty of Agriculture, Brawijaya University. Three types of weed used were: Ageratum conyzoides L. (Asteraceae), Synedrela nodiflora(L.) Gaertn. (Asteraceae), and Arachis pintoi Krap. & Greg. (Fabaceae). The research consisted of two experiments (1) coffee plot with single species of weed and (2) coffee plot with two species of weeds. The results of experiment I and II showed that A. conyzoides, S. nodiflora, A. pintoi and its combination in coffee plantation significantly attracted the predator and parasitoid of CBB. The population of CBB was not significantly different between coffee plot with and without weed. The existence of A. conyzoides, S. nodiflora and A. pintoi in coffee plantations could increase the number of predator and parasitoid of CBB around coffee tree. This result showed that the presence of weeds in coffee plantation is an important factor in maintaining the predators and parasitoid of CBB population.

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CAN COFFEE VARIETY AFFECT THE POPULATION DYNAMICS OF COFFEE BERRY BORER (HYPOTHENEMUS HAMPEI) ON SAO TOME ISLAND

International Journal of Advanced Research ◽

10.21474/ijar01/12487 ◽

2021 ◽

Vol 9 (02) ◽

pp. 592-603

Author(s):

Miclay Carvalho ◽

◽

Alex Lopes ◽

Albino Bento ◽

Luis Santos ◽

...

Keyword(s):

Population Level ◽

Sampling Period ◽

Developmental Rate ◽

Control Measures ◽

Limited Information ◽

Hypothenemus Hampei ◽

Coffee Berry ◽

Coffee Berry Borer ◽

Coffee Plantations ◽

And Control

The coffee berry borer, Hypothenemus hampei, is serious limitation in organic coffee production in Sao Tome Island in the Gulf of Guinea, limited information regarding this species seasonal phenology on the islands limits the implementation of biological control.In the present study, seven coffee plantations were monitored over two years to describe infestation patterns, penetration rates into coffee berries, and mortality by the entomopathogenic fungus,Beauveria bassiana in two coffea species (C. arabica L.and C. canephoraPierre ex A. Froehner), according to 30-tree sampling of fruits following the CENICAFE method. The results revealed that the borer position within the berries, infestation, and green berries with Beauveria bassianawere significantly affected by coffee variety. The infestation mean across all seven sites was (11.5ÃƒÂ‚Ã‚Â±3.5% mean ÃƒÂ‚Ã‚Â± standard error (SE)), with two sites showing mean infestation <5%. The highest infestation and Beauveria bassianarates per branch monthly were observed in C. arabica L. (Red Caturra, Bourbon and Red Catuai), compared with C. canephoraPierre ex A. Froehner (Robusta) in the months of lower rainfall. Borer immature stages (eggs, larvae and pupae) and adults were found throughout the sampling period. No CBB predator and parasitoids were observed in the survey. The population level and developmental rate indicated that the coffee borer met favorable environmental conditions in the studied areas and control measures are required for reducing damage.

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